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The map label for this gene is bioA [C]

Identifier: 83592590

GI number: 83592590

Start: 1475185

End: 1476579

Strand: Reverse

Name: bioA [C]

Synonym: Rru_A1254

Alternate gene names: 83592590

Gene position: 1476579-1475185 (Counterclockwise)

Preceding gene: 83592591

Following gene: 83592589

Centisome position: 33.92

GC content: 64.66

Gene sequence:

>1395_bases
ATGACCGTCGTCGACCGCGGAACCGCCCACTGGCAGAAGCTCGATGCCGATCACCATTGGCATCCCTTCACCAACACCAA
AGCGCTCAACCAGAAGGGCGCGCGGGTCATCGTGCGCGGCGAGGGCGTTTATCTGTGGGATTCCGAGGGCAACCGCATTC
TCGACGGCATGGCCGGGCTGTGGTGCGTCAATCTGGGCTATGGCCGCACCGAACTGGCCGAGGCGGCGGCGGCGCAATTC
GCCACTTTGCCCTTTTACAACACCTTCTTCCAGACCACCCACACCCCCGCCGTCGAACTGGCCCATCTGATCGCCTCGGT
CACCCCGGGCGATCTCAACCATGTGTTCTTCGCCAGTTCCGGTTCGGAAGCCGTCGATACGGCGCTGCGCATGGCCCGGG
CCTATTGGGTGGTGGCCGGCAAGCCCAGCAAGACCTGGGTCATCGGCCGCAAGAACGCCTATCACGGCTCGACCCTGGCC
GGCGCCTCGCTCAGCGGCATGAGCGGCATGTGGAAGTATGGCGCGCCTTTGGTGCCCGATATCGCCCATATCGCCCAGCC
CTATTGGTATGGCGAGGCCGACGCGGCCGAGACCGACCCCGAGGCCTTCGGGCTGGCCCGCGCCCGCCTGCTTGAAGCCA
AGATCCTGGAACTGGGCGCCGAAAACGTCGCCGCCTTCATCGGCGAGCCGATCCAGGGCGCGGGCGGCGTCATCGTGCCG
CCGGCCAGCTATTGGCCCGAGATCGAGCGCATCTGCCGCAAATACGATGTGCTGCTGATCGCCGACGAGGTCATCTGCGG
CTTTGGTCGCACCGGCTCCTGGTTCGGCTCGCAAACCTTTGGCATCACGCCCGATATCATGACCATGGCCAAGGGGCTGT
CGTCGGGCTATCTGCCGATTTCGGCGGTGGCCGTGGGCAACCGGGTGGCCGACGAGTTGATCAATGGCGGGGGCGAATTC
ACCCACGGCTTCACCTATTCGGGCCATCCGGTCTCGGCCGCCGTGGCCAAACGCACAATCGAAATTCTGCGCGATGAAAA
GATCATCGAGCGCGTTCACGACGATATCGGCCCCTATTTCCAAGAGCGCTTGCGCACGCTGGAGGATCACCCGCTGGTCG
GCAAGGTCGATGGCGTCGGCTTGATCGCCGGCATAGCCCTGGTCGCCTCGAAATCGCCCAAACGCTTTTTCGAAAAGGGC
GACGAGGTCGGCCTGATCTGCCGCGACCATTGCTTCGCCGAGGGGTTGGTGATGCGGGCGGTGGGATCGCGCATGGTCTG
CGCCCCGCCGCTGATCATCAGCCGTGACGAGGTCGATGAGCTGGTTCGTCTGGCCCGCCGCTGCTTCGATCTGACCCTGC
GCGATGTCGGCCCCCTGATCGAGACGGCCAAATGA

Upstream 100 bases:

>100_bases
AAAATGTGATCATATTTTTGTGATCACAATTGAGGCATGCCAGATCCTTGCCTAAACCCTGACCTGCCTGTCCCGTCCTT
TCTTTTTGCGGAGTTGGCCC

Downstream 100 bases:

>100_bases
CGGTTCTTCCCCCCGCCGATGGCGGCGCCTTGTTGCGCGAAGAGGTGCGGCGTTTCCGCGAGGCCCATCCCGATATCGCC
TATGTCGATCTGCTGTCGCT

Product: putative aminotransferase

Products: S-Adenosyl-4-methylthio-2-oxobutanoate; 7,8-Diaminononanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTVVDRGTAHWQKLDADHHWHPFTNTKALNQKGARVIVRGEGVYLWDSEGNRILDGMAGLWCVNLGYGRTELAEAAAAQF
ATLPFYNTFFQTTHTPAVELAHLIASVTPGDLNHVFFASSGSEAVDTALRMARAYWVVAGKPSKTWVIGRKNAYHGSTLA
GASLSGMSGMWKYGAPLVPDIAHIAQPYWYGEADAAETDPEAFGLARARLLEAKILELGAENVAAFIGEPIQGAGGVIVP
PASYWPEIERICRKYDVLLIADEVICGFGRTGSWFGSQTFGITPDIMTMAKGLSSGYLPISAVAVGNRVADELINGGGEF
THGFTYSGHPVSAAVAKRTIEILRDEKIIERVHDDIGPYFQERLRTLEDHPLVGKVDGVGLIAGIALVASKSPKRFFEKG
DEVGLICRDHCFAEGLVMRAVGSRMVCAPPLIISRDEVDELVRLARRCFDLTLRDVGPLIETAK

Sequences:

>Translated_464_residues
MTVVDRGTAHWQKLDADHHWHPFTNTKALNQKGARVIVRGEGVYLWDSEGNRILDGMAGLWCVNLGYGRTELAEAAAAQF
ATLPFYNTFFQTTHTPAVELAHLIASVTPGDLNHVFFASSGSEAVDTALRMARAYWVVAGKPSKTWVIGRKNAYHGSTLA
GASLSGMSGMWKYGAPLVPDIAHIAQPYWYGEADAAETDPEAFGLARARLLEAKILELGAENVAAFIGEPIQGAGGVIVP
PASYWPEIERICRKYDVLLIADEVICGFGRTGSWFGSQTFGITPDIMTMAKGLSSGYLPISAVAVGNRVADELINGGGEF
THGFTYSGHPVSAAVAKRTIEILRDEKIIERVHDDIGPYFQERLRTLEDHPLVGKVDGVGLIAGIALVASKSPKRFFEKG
DEVGLICRDHCFAEGLVMRAVGSRMVCAPPLIISRDEVDELVRLARRCFDLTLRDVGPLIETAK
>Mature_463_residues
TVVDRGTAHWQKLDADHHWHPFTNTKALNQKGARVIVRGEGVYLWDSEGNRILDGMAGLWCVNLGYGRTELAEAAAAQFA
TLPFYNTFFQTTHTPAVELAHLIASVTPGDLNHVFFASSGSEAVDTALRMARAYWVVAGKPSKTWVIGRKNAYHGSTLAG
ASLSGMSGMWKYGAPLVPDIAHIAQPYWYGEADAAETDPEAFGLARARLLEAKILELGAENVAAFIGEPIQGAGGVIVPP
ASYWPEIERICRKYDVLLIADEVICGFGRTGSWFGSQTFGITPDIMTMAKGLSSGYLPISAVAVGNRVADELINGGGEFT
HGFTYSGHPVSAAVAKRTIEILRDEKIIERVHDDIGPYFQERLRTLEDHPLVGKVDGVGLIAGIALVASKSPKRFFEKGD
EVGLICRDHCFAEGLVMRAVGSRMVCAPPLIISRDEVDELVRLARRCFDLTLRDVGPLIETAK

Specific function: Biotin biosynthesis. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=429, Percent_Identity=29.8368298368298, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI4557809, Length=438, Percent_Identity=28.9954337899543, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI37574042, Length=367, Percent_Identity=31.0626702997275, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI226442705, Length=364, Percent_Identity=31.3186813186813, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI24119277, Length=371, Percent_Identity=30.7277628032345, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI226442709, Length=335, Percent_Identity=31.044776119403, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI284507298, Length=332, Percent_Identity=30.421686746988, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1786991, Length=441, Percent_Identity=31.9727891156463, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1788044, Length=424, Percent_Identity=31.8396226415094, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI145693181, Length=409, Percent_Identity=30.0733496332518, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789759, Length=405, Percent_Identity=29.1358024691358, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789016, Length=456, Percent_Identity=26.9736842105263, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787560, Length=413, Percent_Identity=29.7820823244552, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786349, Length=434, Percent_Identity=23.0414746543779, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144271, Length=409, Percent_Identity=29.0953545232274, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71992977, Length=440, Percent_Identity=30, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32564660, Length=442, Percent_Identity=30.0904977375566, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI25144274, Length=258, Percent_Identity=32.9457364341085, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17541228, Length=402, Percent_Identity=23.3830845771144, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=460, Percent_Identity=29.7826086956522, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324386, Length=465, Percent_Identity=28.3870967741935, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324432, Length=433, Percent_Identity=27.2517321016166, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21356575, Length=431, Percent_Identity=31.7865429234339, Blast_Score=166, Evalue=5e-41,
Organism=Drosophila melanogaster, GI21357415, Length=434, Percent_Identity=28.3410138248848, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI161085790, Length=451, Percent_Identity=28.6031042128603, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28574759, Length=382, Percent_Identity=30.3664921465969, Blast_Score=146, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62 [C]

Molecular weight: Translated: 50381; Mature: 50250

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVDRGTAHWQKLDADHHWHPFTNTKALNQKGARVIVRGEGVYLWDSEGNRILDGMAGL
CCCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEECCEEEEECCCCCEEHHCCCCE
WCVNLGYGRTELAEAAAAQFATLPFYNTFFQTTHTPAVELAHLIASVTPGDLNHVFFASS
EEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECC
GSEAVDTALRMARAYWVVAGKPSKTWVIGRKNAYHGSTLAGASLSGMSGMWKYGAPLVPD
CCHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
IAHIAQPYWYGEADAAETDPEAFGLARARLLEAKILELGAENVAAFIGEPIQGAGGVIVP
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEEEC
PASYWPEIERICRKYDVLLIADEVICGFGRTGSWFGSQTFGITPDIMTMAKGLSSGYLPI
CHHCCHHHHHHHHHHCEEEEECHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEE
SAVAVGNRVADELINGGGEFTHGFTYSGHPVSAAVAKRTIEILRDEKIIERVHDDIGPYF
EHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QERLRTLEDHPLVGKVDGVGLIAGIALVASKSPKRFFEKGDEVGLICRDHCFAEGLVMRA
HHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHH
VGSRMVCAPPLIISRDEVDELVRLARRCFDLTLRDVGPLIETAK
HCCCCEECCCCEECHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TVVDRGTAHWQKLDADHHWHPFTNTKALNQKGARVIVRGEGVYLWDSEGNRILDGMAGL
CCCCCCCHHHHHCCCCCCCCCCCCCHHHCCCCCEEEEECCEEEEECCCCCEEHHCCCCE
WCVNLGYGRTELAEAAAAQFATLPFYNTFFQTTHTPAVELAHLIASVTPGDLNHVFFASS
EEEECCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECC
GSEAVDTALRMARAYWVVAGKPSKTWVIGRKNAYHGSTLAGASLSGMSGMWKYGAPLVPD
CCHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
IAHIAQPYWYGEADAAETDPEAFGLARARLLEAKILELGAENVAAFIGEPIQGAGGVIVP
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEEEC
PASYWPEIERICRKYDVLLIADEVICGFGRTGSWFGSQTFGITPDIMTMAKGLSSGYLPI
CHHCCHHHHHHHHHHCEEEEECHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEE
SAVAVGNRVADELINGGGEFTHGFTYSGHPVSAAVAKRTIEILRDEKIIERVHDDIGPYF
EHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QERLRTLEDHPLVGKVDGVGLIAGIALVASKSPKRFFEKGDEVGLICRDHCFAEGLVMRA
HHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHH
VGSRMVCAPPLIISRDEVDELVRLARRCFDLTLRDVGPLIETAK
HCCCCEECCCCEECHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Amino-7-oxononanoate; S-Adenosyl-L-methionine [C]

Specific reaction: 8-Amino-7-oxononanoate + S-Adenosyl-L-methionine <==> S-Adenosyl-4-methylthio-2-oxobutanoate + 7,8-Diaminononanoate [C]

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]