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The map label for this gene is sucB [H]

Identifier: 83592550

GI number: 83592550

Start: 1434288

End: 1435583

Strand: Direct

Name: sucB [H]

Synonym: Rru_A1214

Alternate gene names: 83592550

Gene position: 1434288-1435583 (Clockwise)

Preceding gene: 161777626

Following gene: 83592551

Centisome position: 32.95

GC content: 66.59

Gene sequence:

>1296_bases
ATGGCCACCGAAATCATCGTGCCCCAATTGGGCGAGTCCGTGTCCGAAGCGACGGTCGCCAAGTGGTTCAAGAAGGTTGG
CGACGCCGTCGCCGCCGATGAACCCCTTGTCGAGCTTGAGACCGATAAGGTCACCGTCGAGGTTCCGGCCCCGGCCGCTG
GCACGTTGAGCGAGATCATCGCCGCGGAAGGGGCCGAGGTGGCGGTGGGCGCCGTTCTCGCCCTGCTCGCCGGGGCCGGG
GAGGGTGCCGCTCCCGCCAAGCCCGCCGCCGCCCCGGCGGAGAAGAAGGCCGAGCCGGAGAAGAAGCCCGAAGCGGAAAA
GAAGGCGGGGCCGGAAAAGAAGGCCGAGCCGGTGGCCGCCGCCGCGCCCAAGGCCGCCAGCGCCCCTCTCGACTATCCGC
TGCCGCCGGCGGTGCGCAAGCTGGTGGATGACAACGCGCTTGATCCCGCGCGCATTCCCGCCACCGGCAAGGACGGCCGG
TTGACCCGCGACGACGTGGTGGCCTTCCTCAAGGCCGGCGCTCCGGCCGGCGCGCCCGCCAGCGCCCCGGCGCCCGCTAG
CGCTCCGGCGGCGGGTCCGGCCCGCGAGGCCGGTCCGCGCGAGGAAAAGGTCAAGATGACCCGCTTGCGTCGGCGCATCG
CCGAACGTCTGAAGGAGGCCCAGAACACCGCCGCCATCCTGACCACCTTTAACGAGGTGGACATGACCAATCTGATGGCC
CTGCGCAATCAGTACAAGGAAAGCTTCGAGAAGAAGCATGGCGTCAAGCTGGGCTTCATGTCGTTCTTCGTCAAAGCCTG
CGTCAAGGCCTTGCAGGAAATGCCGGCGGTCAATTCCGAGATCAGCGGTGATTCGCTGATCTACAAGAACTACTACGATA
TCGGCGTCGCCGTTGGCGGCGCCCAGGGTCTGGTGGTGCCGGTGGTGCGCGATTGCGACGCCAAGTCCTTCGCCACGATC
GAGGCCGATATCGCCGGCTATGGCAAGAAGGCCCGCGATGGCAGCCTGACCATGGAAGAAATGGCCGGCGGCACCTTCAC
CGTGTCCAACGGCGGGGTCTATGGCTCGCTGATGTCGACGCCGATCATCAACCCGCCGCAGTCGGCCATTCTCGGCATGC
ATAAAACCATGATGCGGCCGATGGTGATGCCCGACGGCTCGATCGCCGCCCGGCCGATGATGTATCTGGCGCTCAGCTAC
GATCACCGCATCGTTGACGGCAAGGAAGCGGTGACCTTCCTGGTCCGCGTCAAGGAATGCATCGAGGATCCGGCCCGCCT
GCTGCTCGACGCCTGA

Upstream 100 bases:

>100_bases
CCAGCCGTTCCGCCGCGCCACCAAGCTCAGCATCATCGGGCAGTGAATTCCCCCCGTTCCTTTCGTCCTGCCCTAGTGCC
ATTCCGGGAGACAGTCAGTC

Downstream 100 bases:

>100_bases
TCCGCAAGGGGAGGGGGGCTTTGGTCGTTCCCCTCCCCATTCCCGAAAATCCACAAGTGCCTGTCCGCCGGGAAGGCCAT
CCCCCGGCGCCTGCAAGGAA

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MATEIIVPQLGESVSEATVAKWFKKVGDAVAADEPLVELETDKVTVEVPAPAAGTLSEIIAAEGAEVAVGAVLALLAGAG
EGAAPAKPAAAPAEKKAEPEKKPEAEKKAGPEKKAEPVAAAAPKAASAPLDYPLPPAVRKLVDDNALDPARIPATGKDGR
LTRDDVVAFLKAGAPAGAPASAPAPASAPAAGPAREAGPREEKVKMTRLRRRIAERLKEAQNTAAILTTFNEVDMTNLMA
LRNQYKESFEKKHGVKLGFMSFFVKACVKALQEMPAVNSEISGDSLIYKNYYDIGVAVGGAQGLVVPVVRDCDAKSFATI
EADIAGYGKKARDGSLTMEEMAGGTFTVSNGGVYGSLMSTPIINPPQSAILGMHKTMMRPMVMPDGSIAARPMMYLALSY
DHRIVDGKEAVTFLVRVKECIEDPARLLLDA

Sequences:

>Translated_431_residues
MATEIIVPQLGESVSEATVAKWFKKVGDAVAADEPLVELETDKVTVEVPAPAAGTLSEIIAAEGAEVAVGAVLALLAGAG
EGAAPAKPAAAPAEKKAEPEKKPEAEKKAGPEKKAEPVAAAAPKAASAPLDYPLPPAVRKLVDDNALDPARIPATGKDGR
LTRDDVVAFLKAGAPAGAPASAPAPASAPAAGPAREAGPREEKVKMTRLRRRIAERLKEAQNTAAILTTFNEVDMTNLMA
LRNQYKESFEKKHGVKLGFMSFFVKACVKALQEMPAVNSEISGDSLIYKNYYDIGVAVGGAQGLVVPVVRDCDAKSFATI
EADIAGYGKKARDGSLTMEEMAGGTFTVSNGGVYGSLMSTPIINPPQSAILGMHKTMMRPMVMPDGSIAARPMMYLALSY
DHRIVDGKEAVTFLVRVKECIEDPARLLLDA
>Mature_430_residues
ATEIIVPQLGESVSEATVAKWFKKVGDAVAADEPLVELETDKVTVEVPAPAAGTLSEIIAAEGAEVAVGAVLALLAGAGE
GAAPAKPAAAPAEKKAEPEKKPEAEKKAGPEKKAEPVAAAAPKAASAPLDYPLPPAVRKLVDDNALDPARIPATGKDGRL
TRDDVVAFLKAGAPAGAPASAPAPASAPAAGPAREAGPREEKVKMTRLRRRIAERLKEAQNTAAILTTFNEVDMTNLMAL
RNQYKESFEKKHGVKLGFMSFFVKACVKALQEMPAVNSEISGDSLIYKNYYDIGVAVGGAQGLVVPVVRDCDAKSFATIE
ADIAGYGKKARDGSLTMEEMAGGTFTVSNGGVYGSLMSTPIINPPQSAILGMHKTMMRPMVMPDGSIAARPMMYLALSYD
HRIVDGKEAVTFLVRVKECIEDPARLLLDA

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=56.6929133858268, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI203098816, Length=459, Percent_Identity=28.5403050108932, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=28.5403050108932, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI31711992, Length=448, Percent_Identity=29.2410714285714, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=25.9090909090909, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=35.4037267080745, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=50.5827505827506, Blast_Score=393, Evalue=1e-111,
Organism=Escherichia coli, GI1786305, Length=435, Percent_Identity=32.4137931034483, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=431, Percent_Identity=43.8515081206497, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=30.6487695749441, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=27.122641509434, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=28.1045751633987, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=439, Percent_Identity=44.1913439635535, Blast_Score=335, Evalue=9e-93,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=28.0434782608696, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24645909, Length=238, Percent_Identity=55.8823529411765, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=31.3084112149533, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=32.4675324675325, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=32.4675324675325, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45155; Mature: 45024

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIIVPQLGESVSEATVAKWFKKVGDAVAADEPLVELETDKVTVEVPAPAAGTLSEII
CCCCEECHHHCCHHHHHHHHHHHHHHCCHHCCCCCCEEEECCEEEEEECCCCCHHHHHHH
AAEGAEVAVGAVLALLAGAGEGAAPAKPAAAPAEKKAEPEKKPEAEKKAGPEKKAEPVAA
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCCHHCCCCHH
AAPKAASAPLDYPLPPAVRKLVDDNALDPARIPATGKDGRLTRDDVVAFLKAGAPAGAPA
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
SAPAPASAPAAGPAREAGPREEKVKMTRLRRRIAERLKEAQNTAAILTTFNEVDMTNLMA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHH
LRNQYKESFEKKHGVKLGFMSFFVKACVKALQEMPAVNSEISGDSLIYKNYYDIGVAVGG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECEEEEEEEECC
AQGLVVPVVRDCDAKSFATIEADIAGYGKKARDGSLTMEEMAGGTFTVSNGGVYGSLMST
CCCEEEEHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCHHHHCCCEEEECCCCEEEHHHCC
PIINPPQSAILGMHKTMMRPMVMPDGSIAARPMMYLALSYDHRIVDGKEAVTFLVRVKEC
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEEECCCEEECCHHHHHHHHHHHHH
IEDPARLLLDA
HHHHHHHHCCC
>Mature Secondary Structure 
ATEIIVPQLGESVSEATVAKWFKKVGDAVAADEPLVELETDKVTVEVPAPAAGTLSEII
CCCEECHHHCCHHHHHHHHHHHHHHCCHHCCCCCCEEEECCEEEEEECCCCCHHHHHHH
AAEGAEVAVGAVLALLAGAGEGAAPAKPAAAPAEKKAEPEKKPEAEKKAGPEKKAEPVAA
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCCHHCCCCHH
AAPKAASAPLDYPLPPAVRKLVDDNALDPARIPATGKDGRLTRDDVVAFLKAGAPAGAPA
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
SAPAPASAPAAGPAREAGPREEKVKMTRLRRRIAERLKEAQNTAAILTTFNEVDMTNLMA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHH
LRNQYKESFEKKHGVKLGFMSFFVKACVKALQEMPAVNSEISGDSLIYKNYYDIGVAVGG
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECEEEEEEEECC
AQGLVVPVVRDCDAKSFATIEADIAGYGKKARDGSLTMEEMAGGTFTVSNGGVYGSLMST
CCCEEEEHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCHHHHCCCEEEECCCCEEEHHHCC
PIINPPQSAILGMHKTMMRPMVMPDGSIAARPMMYLALSYDHRIVDGKEAVTFLVRVKEC
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEEECCCEEECCHHHHHHHHHHHHH
IEDPARLLLDA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA