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The map label for this gene is trmD

Identifier: 83592523

GI number: 83592523

Start: 1404850

End: 1405569

Strand: Direct

Name: trmD

Synonym: Rru_A1187

Alternate gene names: 83592523

Gene position: 1404850-1405569 (Clockwise)

Preceding gene: 83592522

Following gene: 83592524

Centisome position: 32.27

GC content: 65.69

Gene sequence:

>720_bases
ATGAGTTTGCGCGCCGTCGTTCTGACCCTGTTTCCCGAGATGTTTCCCGGACCGCTTGGTCATTCCATCGCCGGCAAGGC
CTTGGAAACCGGCGCTTGGAGCATCGAGGCCGTCGATATCCGGTCGTTTGCCACCGACCGTCACCATACGGTGGACGACA
GTCCCTTTGGCGGTGGCGCCGGCATGGTCATGCGTCCCGACGTGGTGGATGCGGCGATCGCCGCATCCTGGCCGGGCGAT
GGTCCGCTGGTCTATCTGACCCCGCGGGGCCGGGTGCTTGATCAGGCCCTGGTGCGCGAGTTGGCGCAGGCCCCGTCGTT
GACCCTGCTGTGCGGACGCTATGAGGGCGTCGATCAGCGGGTGCTTGACGAACGGGGGATGCTGGAGATCAGCATCGGCG
ACTATGTCCTGTCCGGTGGAGAGGCGGCCGCCCAGGTCCTGCTTGACGCGGTGGTGCGGCTGCTTCCGGGAGTCGTCGGC
AAGACGGAATCCCTTGAAGACGAGAGTTTCGAGCGGGGACTCCTCGAGTATCCCCATTATACGCGGCCCGCCGAATGGCG
GGGCCGCACGGTACCCGAGGTTCTGACCTCGGGCCACCATCAGAAGGTTTCCGACTGGCGACAGGCCCGGTCGGAGGAGA
TCACCCAGACGCGGCGGCCGGACCTGTGGGCCCGCTATGTCGCCGCGCGGACTTCCCTTGATAGAGGATGCGGACAATGA

Upstream 100 bases:

>100_bases
TTCTTGACGACACCCGTCCGCCCGCAGGGGTCGAGGGCGAGGTCGAGGAAGATCCGGGTGTGGGGATCGATGAGGATGGG
GACGGCAAGGGGGGCGCGTC

Downstream 100 bases:

>100_bases
ACATTATCGAGCAGTTCGAGAAGGAGCAGATCGAGAAGCTCCTGGAACAGCGGGGCGTGCCCCAGTTTTCCCCGGGCGAC
ACCCTTCGCGTTCACGTGAA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSLRAVVLTLFPEMFPGPLGHSIAGKALETGAWSIEAVDIRSFATDRHHTVDDSPFGGGAGMVMRPDVVDAAIAASWPGD
GPLVYLTPRGRVLDQALVRELAQAPSLTLLCGRYEGVDQRVLDERGMLEISIGDYVLSGGEAAAQVLLDAVVRLLPGVVG
KTESLEDESFERGLLEYPHYTRPAEWRGRTVPEVLTSGHHQKVSDWRQARSEEITQTRRPDLWARYVAARTSLDRGCGQ

Sequences:

>Translated_239_residues
MSLRAVVLTLFPEMFPGPLGHSIAGKALETGAWSIEAVDIRSFATDRHHTVDDSPFGGGAGMVMRPDVVDAAIAASWPGD
GPLVYLTPRGRVLDQALVRELAQAPSLTLLCGRYEGVDQRVLDERGMLEISIGDYVLSGGEAAAQVLLDAVVRLLPGVVG
KTESLEDESFERGLLEYPHYTRPAEWRGRTVPEVLTSGHHQKVSDWRQARSEEITQTRRPDLWARYVAARTSLDRGCGQ
>Mature_238_residues
SLRAVVLTLFPEMFPGPLGHSIAGKALETGAWSIEAVDIRSFATDRHHTVDDSPFGGGAGMVMRPDVVDAAIAASWPGDG
PLVYLTPRGRVLDQALVRELAQAPSLTLLCGRYEGVDQRVLDERGMLEISIGDYVLSGGEAAAQVLLDAVVRLLPGVVGK
TESLEDESFERGLLEYPHYTRPAEWRGRTVPEVLTSGHHQKVSDWRQARSEEITQTRRPDLWARYVAARTSLDRGCGQ

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=219, Percent_Identity=46.5753424657534, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_RHORT (Q2RV57)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426275.1
- ProteinModelPortal:   Q2RV57
- SMR:   Q2RV57
- STRING:   Q2RV57
- GeneID:   3834697
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1187
- NMPDR:   fig|1085.1.peg.3179
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- PhylomeDB:   Q2RV57
- ProtClustDB:   PRK00026
- BioCyc:   RRUB269796:RRU_A1187-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26131; Mature: 26000

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRAVVLTLFPEMFPGPLGHSIAGKALETGAWSIEAVDIRSFATDRHHTVDDSPFGGGA
CCHHHHHHHHHHHHCCCCCCCHHHCHHHHCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCC
GMVMRPDVVDAAIAASWPGDGPLVYLTPRGRVLDQALVRELAQAPSLTLLCGRYEGVDQR
CCEECCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
VLDERGMLEISIGDYVLSGGEAAAQVLLDAVVRLLPGVVGKTESLEDESFERGLLEYPHY
HHCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
TRPAEWRGRTVPEVLTSGHHQKVSDWRQARSEEITQTRRPDLWARYVAARTSLDRGCGQ
CCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SLRAVVLTLFPEMFPGPLGHSIAGKALETGAWSIEAVDIRSFATDRHHTVDDSPFGGGA
CHHHHHHHHHHHHCCCCCCCHHHCHHHHCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCC
GMVMRPDVVDAAIAASWPGDGPLVYLTPRGRVLDQALVRELAQAPSLTLLCGRYEGVDQR
CCEECCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
VLDERGMLEISIGDYVLSGGEAAAQVLLDAVVRLLPGVVGKTESLEDESFERGLLEYPHY
HHCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCC
TRPAEWRGRTVPEVLTSGHHQKVSDWRQARSEEITQTRRPDLWARYVAARTSLDRGCGQ
CCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA