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The map label for this gene is amtB [H]

Identifier: 83592465

GI number: 83592465

Start: 1330513

End: 1331808

Strand: Reverse

Name: amtB [H]

Synonym: Rru_A1129

Alternate gene names: 83592465

Gene position: 1331808-1330513 (Counterclockwise)

Preceding gene: 83592466

Following gene: 83592455

Centisome position: 30.6

GC content: 66.51

Gene sequence:

>1296_bases
ATGATGAGAGCCTTCACGTTCCTCCCCGCCGCCCTGGTCGCCCTTCTGGCGCCGGCCCTGGCCTATGCCCAGGATGCGGC
CCCCACCCTGGATTCGGGCAATACCGCCTGGATGCTGACCTCGACCGCGCTGGTCCTTCTGATGACCATCCCGGGCCTCG
CGCTTTTCTATGCCGGCATGGTCCGCAAGAAGAACGTGCTGGCGACCATGATGCAGAGCTTCATCATCACCGCGCTGGTC
AGCGTCGTGTGGATGGTGGCCGGCTACTCGATCGCCTTTGGCACCGGCAACGCCTATTTCGGTGACTTCTCGAAGTTCCT
GCTGTCGGACATCAGCATTGACAGCCTGAACGGCACCTTCCCCGAAACGGTGTTCGTCGTCTTCCAGATGACCTTCGCCA
TCATCACCCCGGCCCTGATCACCGGCGCCTTCGCCGAACGCATGAAGTTCTCGGCGATGCTGTGGTTCATCGGCCTGTGG
GTGCTGATCGTCTACGCGCCGATCTGCCATTGGGTGTGGAGTTCCTCGGGCTTCCTGTTCAACGCCGGCGTCCTTGATTA
CGCGGGCGGCACCGTGGTTCACATCAACGCCGGCGTGGCCGGTCTGGTCTGCGCCCTCGTCTTGCGTCGTCGCGCCGGCT
TCGGCAGCGAGAACATGGCCCCCTATAACCTGTCGCTGGCCGTGATCGGCGCCGCCCTGCTGTGGGTGGGCTGGTTCGGC
TTCAACGCCGGGTCCGCCGTCGCCGCCGATGGCCGTGCCGGCATGGCCATGCTGGCGACCCAGCTTGGCGCCGCCGGTGC
CACCTTGTCGTGGATCGCCGCCGAATGGCTGGTGCGCGGCAAGCCCAGCGTGCTGGGCGCGGTTTCGGGCGCCGTCGCCG
GTCTGGTCGCCATCACCCCGGCCTCGGGCTTCGTTCTGCCCGGTGGCGCCCTGGTCATCGGCCTGGTGGCCGGCGTCGTC
TGCTTCTGGGGCGCGACTTGGCTGAAGAAGACCCTGGGCTATGACGACAGCCTGGACGCCTTCGGCGTGCATGGCATCGG
CGGTCTGGTCGGCGCCCTGCTGACCGGCGTCTTCGCCGCCCAGAGCGTTGGTGGCGAAGCCGCCGTCGGCCTGCTCGAAG
GCAATCCCGGTCAGGTCCTCACCCAGGCCTGGGGCTGCCTTGCCACCATCGTGTGGACGGCCGTCGCCTCCTACGTGATC
TTGAAGGTCATTGATCTGGCCATCGGCCTGCGGGTTTCGCCCGAGCAGGAAAGCGAAGGCCTCGACATCTCGCTGCACGG
CGAGCGCCTGCAGTAA

Upstream 100 bases:

>100_bases
GCCGGGTGGGATGTGGGTCGTGGCACGGTTCTTGTTACGACACTCCCCGCCGGAGGGCGGAAGACCTCCCGATCAGCCAG
CTCCTGTGAGACAAACCACC

Downstream 100 bases:

>100_bases
GCGATCACACACATTCGATGGAAACGGGGGCGCTCCGCGGGGCGCCCCCTTTTTTTTCAGGACGACAGAGTGTGTGGTCC
CTGGGTGAGATGGTGCGGGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MMRAFTFLPAALVALLAPALAYAQDAAPTLDSGNTAWMLTSTALVLLMTIPGLALFYAGMVRKKNVLATMMQSFIITALV
SVVWMVAGYSIAFGTGNAYFGDFSKFLLSDISIDSLNGTFPETVFVVFQMTFAIITPALITGAFAERMKFSAMLWFIGLW
VLIVYAPICHWVWSSSGFLFNAGVLDYAGGTVVHINAGVAGLVCALVLRRRAGFGSENMAPYNLSLAVIGAALLWVGWFG
FNAGSAVAADGRAGMAMLATQLGAAGATLSWIAAEWLVRGKPSVLGAVSGAVAGLVAITPASGFVLPGGALVIGLVAGVV
CFWGATWLKKTLGYDDSLDAFGVHGIGGLVGALLTGVFAAQSVGGEAAVGLLEGNPGQVLTQAWGCLATIVWTAVASYVI
LKVIDLAIGLRVSPEQESEGLDISLHGERLQ

Sequences:

>Translated_431_residues
MMRAFTFLPAALVALLAPALAYAQDAAPTLDSGNTAWMLTSTALVLLMTIPGLALFYAGMVRKKNVLATMMQSFIITALV
SVVWMVAGYSIAFGTGNAYFGDFSKFLLSDISIDSLNGTFPETVFVVFQMTFAIITPALITGAFAERMKFSAMLWFIGLW
VLIVYAPICHWVWSSSGFLFNAGVLDYAGGTVVHINAGVAGLVCALVLRRRAGFGSENMAPYNLSLAVIGAALLWVGWFG
FNAGSAVAADGRAGMAMLATQLGAAGATLSWIAAEWLVRGKPSVLGAVSGAVAGLVAITPASGFVLPGGALVIGLVAGVV
CFWGATWLKKTLGYDDSLDAFGVHGIGGLVGALLTGVFAAQSVGGEAAVGLLEGNPGQVLTQAWGCLATIVWTAVASYVI
LKVIDLAIGLRVSPEQESEGLDISLHGERLQ
>Mature_431_residues
MMRAFTFLPAALVALLAPALAYAQDAAPTLDSGNTAWMLTSTALVLLMTIPGLALFYAGMVRKKNVLATMMQSFIITALV
SVVWMVAGYSIAFGTGNAYFGDFSKFLLSDISIDSLNGTFPETVFVVFQMTFAIITPALITGAFAERMKFSAMLWFIGLW
VLIVYAPICHWVWSSSGFLFNAGVLDYAGGTVVHINAGVAGLVCALVLRRRAGFGSENMAPYNLSLAVIGAALLWVGWFG
FNAGSAVAADGRAGMAMLATQLGAAGATLSWIAAEWLVRGKPSVLGAVSGAVAGLVAITPASGFVLPGGALVIGLVAGVV
CFWGATWLKKTLGYDDSLDAFGVHGIGGLVGALLTGVFAAQSVGGEAAVGLLEGNPGQVLTQAWGCLATIVWTAVASYVI
LKVIDLAIGLRVSPEQESEGLDISLHGERLQ

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=407, Percent_Identity=50.3685503685504, Blast_Score=387, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17550182, Length=434, Percent_Identity=28.3410138248848, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=428, Percent_Identity=27.1028037383178, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=438, Percent_Identity=26.4840182648402, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=431, Percent_Identity=25.9860788863109, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324187, Length=426, Percent_Identity=36.3849765258216, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=36.7788461538462, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=411, Percent_Identity=33.3333333333333, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI161078314, Length=412, Percent_Identity=30.5825242718447, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44777; Mature: 44777

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRAFTFLPAALVALLAPALAYAQDAAPTLDSGNTAWMLTSTALVLLMTIPGLALFYAGM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
VRKKNVLATMMQSFIITALVSVVWMVAGYSIAFGTGNAYFGDFSKFLLSDISIDSLNGTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
PETVFVVFQMTFAIITPALITGAFAERMKFSAMLWFIGLWVLIVYAPICHWVWSSSGFLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
NAGVLDYAGGTVVHINAGVAGLVCALVLRRRAGFGSENMAPYNLSLAVIGAALLWVGWFG
ECCHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
FNAGSAVAADGRAGMAMLATQLGAAGATLSWIAAEWLVRGKPSVLGAVSGAVAGLVAITP
CCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECC
ASGFVLPGGALVIGLVAGVVCFWGATWLKKTLGYDDSLDAFGVHGIGGLVGALLTGVFAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QSVGGEAAVGLLEGNPGQVLTQAWGCLATIVWTAVASYVILKVIDLAIGLRVSPEQESEG
HHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LDISLHGERLQ
CEEEECCCCCC
>Mature Secondary Structure
MMRAFTFLPAALVALLAPALAYAQDAAPTLDSGNTAWMLTSTALVLLMTIPGLALFYAGM
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
VRKKNVLATMMQSFIITALVSVVWMVAGYSIAFGTGNAYFGDFSKFLLSDISIDSLNGTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCC
PETVFVVFQMTFAIITPALITGAFAERMKFSAMLWFIGLWVLIVYAPICHWVWSSSGFLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
NAGVLDYAGGTVVHINAGVAGLVCALVLRRRAGFGSENMAPYNLSLAVIGAALLWVGWFG
ECCHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
FNAGSAVAADGRAGMAMLATQLGAAGATLSWIAAEWLVRGKPSVLGAVSGAVAGLVAITP
CCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEECC
ASGFVLPGGALVIGLVAGVVCFWGATWLKKTLGYDDSLDAFGVHGIGGLVGALLTGVFAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QSVGGEAAVGLLEGNPGQVLTQAWGCLATIVWTAVASYVILKVIDLAIGLRVSPEQESEG
HHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LDISLHGERLQ
CEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]