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The map label for this gene is thiDE [H]

Identifier: 83592438

GI number: 83592438

Start: 1294737

End: 1295552

Strand: Direct

Name: thiDE [H]

Synonym: Rru_A1102

Alternate gene names: 83592438

Gene position: 1294737-1295552 (Clockwise)

Preceding gene: 83592436

Following gene: 83592439

Centisome position: 29.74

GC content: 67.03

Gene sequence:

>816_bases
ATGACGCAAGGGCGCGTATTGATCATAGCGGGATCGGATTCAAGCGGCGGTGCCGGAATCCAGGCCGACCTCAAGGCGGT
GATGGCCCTGGGTGGCTATGGCGCCACGGCGATCGCCGCCCTGACCGCCCAGAACACCCGGGGCGTCGAAGGCATCCATG
CGGTTCCGCCGGCCTTTGTCCGCCAGCAGATGGCTCTGTTCCTTGATGATATCGGTGCCGACGCCCTGAAGATCGGCATG
CTGGCGACCGCTGAGGTCATCGAGGCGGTGGCCGATGAAATCGAATTGCGCGCGGCGGGGCTTCCGCTGGTCGTCGATCC
GGTGATGGTCGCCAAGGGCGGCCACCGGCTGCTTGAGGAAAGCGCCACGGCGGCGTTGATCGCCCGCCTGTTGCCGATCG
CCCGGGTGCTGACCCCCAATCTGCCCGAGGCCGAGGTGCTGGTCGGCTTTTCTATCGCCGACGAGGCGGCGATGGAAAAG
GCCGGGCGCGCCCTGCTTGAACGCGGTCCGCAAGCCGTGCTGATGAAGGGTGGGCATCTGGAGGGCGACGATCTCGTCGA
TCTTCTGGTGACCGCCGAGGGCACCGTGCGCTTTCGCGCCTCGCGCTTGCAGACAACATCGACCCATGGCACCGGCTGCA
CCCTGGCCTCGGCCATCGCCTGCGGTCTGGCCCAGGGGCGGTCGGTCGAACAAGCCATCGCCCGCGCCCGCGCCTATGTG
CGCAAGGCCATGGAAACCGCGCCCGGTCTCGGTCACGGCCATGGCCCGCTCAATCACGGATTTACCGTTTCCCCCTTCGA
GGTGCCCTTGCCATGA

Upstream 100 bases:

>100_bases
TCGGAACCCGCTACCAGTTCCGAACGGTTTTCGGATGCGGTCTTGACGTAAGGCCGGGCGGCGGGCACCAGAGGGGCCCG
GAACAAGCGGAGGGAAGCCA

Downstream 100 bases:

>100_bases
CCGCCGCCTTTGCCGCCGGCCCCCGTTTGCGCGTGCCGTTCTTCCAGGTCGACGCCTTCGCCGATGTCGCCTTCGCCGGC
AATCCGGCGGCGGTCTGCCT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTQGRVLIIAGSDSSGGAGIQADLKAVMALGGYGATAIAALTAQNTRGVEGIHAVPPAFVRQQMALFLDDIGADALKIGM
LATAEVIEAVADEIELRAAGLPLVVDPVMVAKGGHRLLEESATAALIARLLPIARVLTPNLPEAEVLVGFSIADEAAMEK
AGRALLERGPQAVLMKGGHLEGDDLVDLLVTAEGTVRFRASRLQTTSTHGTGCTLASAIACGLAQGRSVEQAIARARAYV
RKAMETAPGLGHGHGPLNHGFTVSPFEVPLP

Sequences:

>Translated_271_residues
MTQGRVLIIAGSDSSGGAGIQADLKAVMALGGYGATAIAALTAQNTRGVEGIHAVPPAFVRQQMALFLDDIGADALKIGM
LATAEVIEAVADEIELRAAGLPLVVDPVMVAKGGHRLLEESATAALIARLLPIARVLTPNLPEAEVLVGFSIADEAAMEK
AGRALLERGPQAVLMKGGHLEGDDLVDLLVTAEGTVRFRASRLQTTSTHGTGCTLASAIACGLAQGRSVEQAIARARAYV
RKAMETAPGLGHGHGPLNHGFTVSPFEVPLP
>Mature_270_residues
TQGRVLIIAGSDSSGGAGIQADLKAVMALGGYGATAIAALTAQNTRGVEGIHAVPPAFVRQQMALFLDDIGADALKIGML
ATAEVIEAVADEIELRAAGLPLVVDPVMVAKGGHRLLEESATAALIARLLPIARVLTPNLPEAEVLVGFSIADEAAMEKA
GRALLERGPQAVLMKGGHLEGDDLVDLLVTAEGTVRFRASRLQTTSTHGTGCTLASAIACGLAQGRSVEQAIARARAYVR
KAMETAPGLGHGHGPLNHGFTVSPFEVPLP

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=262, Percent_Identity=46.1832061068702, Blast_Score=209, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6325378, Length=283, Percent_Identity=34.2756183745583, Blast_Score=142, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324517, Length=289, Percent_Identity=35.9861591695502, Blast_Score=126, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324997, Length=289, Percent_Identity=35.9861591695502, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27844; Mature: 27712

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQGRVLIIAGSDSSGGAGIQADLKAVMALGGYGATAIAALTAQNTRGVEGIHAVPPAFV
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
RQQMALFLDDIGADALKIGMLATAEVIEAVADEIELRAAGLPLVVDPVMVAKGGHRLLEE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCHHHHHH
SATAALIARLLPIARVLTPNLPEAEVLVGFSIADEAAMEKAGRALLERGPQAVLMKGGHL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
EGDDLVDLLVTAEGTVRFRASRLQTTSTHGTGCTLASAIACGLAQGRSVEQAIARARAYV
CCHHHEEEEEECCCCEEEEHHHHEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RKAMETAPGLGHGHGPLNHGFTVSPFEVPLP
HHHHHHCCCCCCCCCCCCCCCEECCCCCCCC
>Mature Secondary Structure 
TQGRVLIIAGSDSSGGAGIQADLKAVMALGGYGATAIAALTAQNTRGVEGIHAVPPAFV
CCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHH
RQQMALFLDDIGADALKIGMLATAEVIEAVADEIELRAAGLPLVVDPVMVAKGGHRLLEE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHCCCHHHHHH
SATAALIARLLPIARVLTPNLPEAEVLVGFSIADEAAMEKAGRALLERGPQAVLMKGGHL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
EGDDLVDLLVTAEGTVRFRASRLQTTSTHGTGCTLASAIACGLAQGRSVEQAIARARAYV
CCHHHEEEEEECCCCEEEEHHHHEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RKAMETAPGLGHGHGPLNHGFTVSPFEVPLP
HHHHHHCCCCCCCCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA