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The map label for this gene is def1 [H]

Identifier: 83592380

GI number: 83592380

Start: 1236659

End: 1237168

Strand: Direct

Name: def1 [H]

Synonym: Rru_A1044

Alternate gene names: 83592380

Gene position: 1236659-1237168 (Clockwise)

Preceding gene: 83592378

Following gene: 83592381

Centisome position: 28.41

GC content: 65.1

Gene sequence:

>510_bases
ATGCCGGTTTTGGCGGGAATCGCCCGACCGGTCGAAGATCCCACCGACCCAAAGATCCACCGTCTGGTGGCCGATATGAT
CGAGACCCTGGCCGACTCGGGTGGGGTCGGCCTTGCCGCGCCCCAGGTCCATGTGCCCTTGCGGGTGATGATCTTCCACG
TTCCGGCCAACCGCTCGACCGAGGCCGAGGGGGCGGTGCCGCTGACCGTTCTGATCAATCCGCAACTGACGCCGCTGGGC
GAAGAGATGGTCGAGGATTGGGAGGGCTGCCTGTCGCTGCCCGGGCTCACCGGTCTGGTGCCCCGCTATCGGTCCTTGCG
CTATCGCGGCGTCGATCTGGACGGGCGGGAGGTCGAACGCGAAGCCCGCGATTTCCATGCCCGGGTCGTTCAGCACGAAT
ATGACCATCTTGACGGTGTGCTTTATCCGCGCCGTCTGCGCGATCCCAGCCAGTTCGGTTTCGTCGAAGAAATTCGCAAG
GCGCTGGCGGCCAACCGCGAGCAGCCATAG

Upstream 100 bases:

>100_bases
GGGTCGAGGCGGGCTTTTTAGCATGGAACGGCGGTTTACCCAAGAGGCCAGCGCGGGCATAATCGCCGCCATGGCTATTC
TGAAGATCGCAAGGATGGGA

Downstream 100 bases:

>100_bases
ATTTAAGGACGCGGGGATTTTCCGCGCCCACCGCTCGAGCCCCCTTTAACGCCGGGACCAGGGTCGATTTGGTCCCGGCC
CGCGCTTTTGGTCTGGAGAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MPVLAGIARPVEDPTDPKIHRLVADMIETLADSGGVGLAAPQVHVPLRVMIFHVPANRSTEAEGAVPLTVLINPQLTPLG
EEMVEDWEGCLSLPGLTGLVPRYRSLRYRGVDLDGREVEREARDFHARVVQHEYDHLDGVLYPRRLRDPSQFGFVEEIRK
ALAANREQP

Sequences:

>Translated_169_residues
MPVLAGIARPVEDPTDPKIHRLVADMIETLADSGGVGLAAPQVHVPLRVMIFHVPANRSTEAEGAVPLTVLINPQLTPLG
EEMVEDWEGCLSLPGLTGLVPRYRSLRYRGVDLDGREVEREARDFHARVVQHEYDHLDGVLYPRRLRDPSQFGFVEEIRK
ALAANREQP
>Mature_168_residues
PVLAGIARPVEDPTDPKIHRLVADMIETLADSGGVGLAAPQVHVPLRVMIFHVPANRSTEAEGAVPLTVLINPQLTPLGE
EMVEDWEGCLSLPGLTGLVPRYRSLRYRGVDLDGREVEREARDFHARVVQHEYDHLDGVLYPRRLRDPSQFGFVEEIRKA
LAANREQP

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=152, Percent_Identity=34.8684210526316, Blast_Score=94, Evalue=7e-20,
Organism=Escherichia coli, GI1789682, Length=140, Percent_Identity=41.4285714285714, Blast_Score=94, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24645728, Length=163, Percent_Identity=33.1288343558282, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24645726, Length=153, Percent_Identity=33.9869281045752, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18843; Mature: 18712

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLAGIARPVEDPTDPKIHRLVADMIETLADSGGVGLAAPQVHVPLRVMIFHVPANRST
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCC
EAEGAVPLTVLINPQLTPLGEEMVEDWEGCLSLPGLTGLVPRYRSLRYRGVDLDGREVER
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCEECCCCCCHHHHHH
EARDFHARVVQHEYDHLDGVLYPRRLRDPSQFGFVEEIRKALAANREQP
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PVLAGIARPVEDPTDPKIHRLVADMIETLADSGGVGLAAPQVHVPLRVMIFHVPANRST
CCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCC
EAEGAVPLTVLINPQLTPLGEEMVEDWEGCLSLPGLTGLVPRYRSLRYRGVDLDGREVER
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCEECCCCCCHHHHHH
EARDFHARVVQHEYDHLDGVLYPRRLRDPSQFGFVEEIRKALAANREQP
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]