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The map label for this gene is mraW

Identifier: 83592294

GI number: 83592294

Start: 1143679

End: 1144659

Strand: Reverse

Name: mraW

Synonym: Rru_A0958

Alternate gene names: 83592294

Gene position: 1144659-1143679 (Counterclockwise)

Preceding gene: 83592295

Following gene: 83592293

Centisome position: 26.3

GC content: 69.42

Gene sequence:

>981_bases
ATGAACGCCCTCACCCCCCATTTCCCGGTGATGCTCGACGAGGTGCTAAGCGCCCTCGAGCCCCATGCCGACGGCCTTTA
TGTCGATGGCACCTTCGGCAACGGCGGGTATAGCCGTGGCCTGCTCGAGCGCGCCGACTGCCGGGTGATCGCCATCGACC
GCGATCCGGCCGCCCGCGTGCGCGGCGCCGCCCTGGAAGCCGAGTTCCCCGGCCGCTTCACCCTGCTTTCGGGCTGCTTT
GGCGACATGGAACAGCTGCTGGCCGCCGAGGGCATCAGCCGGATCGACGGCGTCGCCCTGGATCTTGGCGTCTCGTCGAT
GCAGCTCGATCAGGCCGAGCGCGGCTTCAGCTTCCAGAAGGACGGCCCGCTCGACATGCGCATGGGCCAGCAGGGGCAGA
CGGCGGCCGATCTGGTCAATACCCTCGACGAGGACCGCCTCGCCGATATCCTCTACCACTACGGCGAAGAGCGGAAATCA
CGCTGGGTCGCCCATGCCATCGTCGAACGCCGCGCCGAGACGCCGTTCTCGCGCACCGCCGATCTGGCCACCGTCATCCG
CGGCGTCGTCCGCAAAAGCCGCGATGGCATCGATCCCGCCACCCGCAGCTTCCAGGCGCTGCGCATCGTCGTCAACGACG
AGCTGGGCGAGCTTGAACGCGCCCTTGGCGCCAGCGAACGCCTGCTGCGCGCCGGTGGCCGGCTGGTGGTCGTGGCCTTT
CATTCGCTTGAGGATCGCATCGTCAAGGCGTTCGTCCGCCAGCGCTGCGGCGAAAGCGAGGGCGTCAGCCGCCACCTGCC
CCAGGCCTCGAACGCCGGAGCGGGCAATCCTCCCCCCAGTTTCCAGGCCGTCTCGCGCCGCGCCGTCAAGCCGCTCGACG
CCGAGACCCGGGTCAACCCGCGGTCCCGCTCGGCCCGGCTGCGCGCCGTCGAACGCACCGAATTTCCCGCCTGGACGGTT
TCCCCCGGAGGTCAGCGATGA

Upstream 100 bases:

>100_bases
TCGTCGGTCTTGGCCGGGTCTTCCAGATCTGGAACCCAACGGCCTTGGAAGCCTCGCGCGGCCGCAAGCCGCGGGCCTCC
GCGTCCCAGGGTGCCGGCGC

Downstream 100 bases:

>100_bases
TCCGCCCCATCCACATCATCTGGGCCGCGTTGATCATGGGCATCGGCACCGCGCTGTTCATGGTCGCCTATGAGGTCGAT
GCCAAGGAAAAGGAACTGGC

Product: s-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNALTPHFPVMLDEVLSALEPHADGLYVDGTFGNGGYSRGLLERADCRVIAIDRDPAARVRGAALEAEFPGRFTLLSGCF
GDMEQLLAAEGISRIDGVALDLGVSSMQLDQAERGFSFQKDGPLDMRMGQQGQTAADLVNTLDEDRLADILYHYGEERKS
RWVAHAIVERRAETPFSRTADLATVIRGVVRKSRDGIDPATRSFQALRIVVNDELGELERALGASERLLRAGGRLVVVAF
HSLEDRIVKAFVRQRCGESEGVSRHLPQASNAGAGNPPPSFQAVSRRAVKPLDAETRVNPRSRSARLRAVERTEFPAWTV
SPGGQR

Sequences:

>Translated_326_residues
MNALTPHFPVMLDEVLSALEPHADGLYVDGTFGNGGYSRGLLERADCRVIAIDRDPAARVRGAALEAEFPGRFTLLSGCF
GDMEQLLAAEGISRIDGVALDLGVSSMQLDQAERGFSFQKDGPLDMRMGQQGQTAADLVNTLDEDRLADILYHYGEERKS
RWVAHAIVERRAETPFSRTADLATVIRGVVRKSRDGIDPATRSFQALRIVVNDELGELERALGASERLLRAGGRLVVVAF
HSLEDRIVKAFVRQRCGESEGVSRHLPQASNAGAGNPPPSFQAVSRRAVKPLDAETRVNPRSRSARLRAVERTEFPAWTV
SPGGQR
>Mature_326_residues
MNALTPHFPVMLDEVLSALEPHADGLYVDGTFGNGGYSRGLLERADCRVIAIDRDPAARVRGAALEAEFPGRFTLLSGCF
GDMEQLLAAEGISRIDGVALDLGVSSMQLDQAERGFSFQKDGPLDMRMGQQGQTAADLVNTLDEDRLADILYHYGEERKS
RWVAHAIVERRAETPFSRTADLATVIRGVVRKSRDGIDPATRSFQALRIVVNDELGELERALGASERLLRAGGRLVVVAF
HSLEDRIVKAFVRQRCGESEGVSRHLPQASNAGAGNPPPSFQAVSRRAVKPLDAETRVNPRSRSARLRAVERTEFPAWTV
SPGGQR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=332, Percent_Identity=40.0602409638554, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=44.3850267379679, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1786270, Length=311, Percent_Identity=44.3729903536978, Blast_Score=228, Evalue=6e-61,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=38.0664652567976, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=38.0664652567976, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_RHORT (Q2RVT6)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426046.1
- ProteinModelPortal:   Q2RVT6
- SMR:   Q2RVT6
- STRING:   Q2RVT6
- GeneID:   3833631
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0958
- NMPDR:   fig|1085.1.peg.3414
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q2RVT6
- ProtClustDB:   PRK00050
- BioCyc:   RRUB269796:RRU_A0958-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35629; Mature: 35629

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: BINDING 53-53 BINDING 80-80 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALTPHFPVMLDEVLSALEPHADGLYVDGTFGNGGYSRGLLERADCRVIAIDRDPAARV
CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCHHHCCEEEEEECCCCHHHH
RGAALEAEFPGRFTLLSGCFGDMEQLLAAEGISRIDGVALDLGVSSMQLDQAERGFSFQK
HCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCCC
DGPLDMRMGQQGQTAADLVNTLDEDRLADILYHYGEERKSRWVAHAIVERRAETPFSRTA
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DLATVIRGVVRKSRDGIDPATRSFQALRIVVNDELGELERALGASERLLRAGGRLVVVAF
HHHHHHHHHHHHHCCCCCHHHHHHHHEEEEECCCHHHHHHHHCHHHHHHHCCCCEEEEEH
HSLEDRIVKAFVRQRCGESEGVSRHLPQASNAGAGNPPPSFQAVSRRAVKPLDAETRVNP
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
RSRSARLRAVERTEFPAWTVSPGGQR
CHHHHHHHHHHHCCCCEEECCCCCCC
>Mature Secondary Structure
MNALTPHFPVMLDEVLSALEPHADGLYVDGTFGNGGYSRGLLERADCRVIAIDRDPAARV
CCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCHHHCCEEEEEECCCCHHHH
RGAALEAEFPGRFTLLSGCFGDMEQLLAAEGISRIDGVALDLGVSSMQLDQAERGFSFQK
HCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCCC
DGPLDMRMGQQGQTAADLVNTLDEDRLADILYHYGEERKSRWVAHAIVERRAETPFSRTA
CCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DLATVIRGVVRKSRDGIDPATRSFQALRIVVNDELGELERALGASERLLRAGGRLVVVAF
HHHHHHHHHHHHHCCCCCHHHHHHHHEEEEECCCHHHHHHHHCHHHHHHHCCCCEEEEEH
HSLEDRIVKAFVRQRCGESEGVSRHLPQASNAGAGNPPPSFQAVSRRAVKPLDAETRVNP
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
RSRSARLRAVERTEFPAWTVSPGGQR
CHHHHHHHHHHHCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA