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The map label for this gene is ligA

Identifier: 83592275

GI number: 83592275

Start: 1118665

End: 1120761

Strand: Reverse

Name: ligA

Synonym: Rru_A0939

Alternate gene names: 83592275

Gene position: 1120761-1118665 (Counterclockwise)

Preceding gene: 83592276

Following gene: 83592274

Centisome position: 25.75

GC content: 66.95

Gene sequence:

>2097_bases
ATGATTCCCGTCGCCGATCTCACCGAGGCCGAGGCCGCCCGCGAACTCGAACGGCTTGCCGCCGAGATCGCCCGCCACGA
CCGGCTCTATCACCAGCAGGACGCGCCGGAAATCACCGACGGCGAGTACGACGCCCTGGTACGGCGCAATAACGAGATCG
AGGCCGCCTTCCCCGAACTGGTGCGCGGCGACAGCCCCTCGGCCCATGTCGGCGCCGCTCCGGCCGAAGGCTTCGCCAAG
GTCACCCACGCCCTGCCGATGCTGTCGCTGGGCAATGTGTTCAGCGACGACGAGGCCCGCGAGTTCGACCAGCGCATCCG
CCGCTTCCTCAGCCTGGATGGCGAAACGACGATCAGCTATGTCGCCGAGCCCAAGATCGATGGCCTGTCGTTTTCGGCGC
GCTACGAGAACGGCGTCTATCGCCGCGCCGCCACCCGGGGTGATGGCACCACCGGCGAGGACATCACCGCCAATCTGGCG
ACCCTGACCGATCTGCCCCAGCGCCTGACAGGCGACGGGCCTGTGCCCGAGGTGCTGGAGGTGCGCGGCGAGGTGTTCAT
GACCAAGGCCGATTTCCGCGCCCTCAACCAACGCCAGATCGACGCCGGCGCCAAGCCCTTCGCCAATCCGCGCAATGCGG
CGGCCGGGTCGCTGCGCCAGCTTGATCCCGAAATCACCCGCTCGCGCTCGTTGAGCCTGTTCGCCTATGCCTCTGGCGAG
GTCAAGGGGCTGGAGGTGGAGAGCCATCACGCCTTTCTAGAGCGCCTGAAAACCTGGGGGTTCCCGGTCAATCCCCAAAC
CCGCCCCTGCCAGGGGGTGGAAGACCTGATCGAAGCCACCCACGCGTTGGCTTTGCAACGGGCGGCGCTGGATTATGATA
TCGACGGCGTCGTCTATAAGGTCGACCGCGTCGATTGGCAAAAGCGCCTGGGCTTCGTCAGCCGCGCCCCGCGCTGGGCG
ATCGCCCGCAAATTCCCCGCCGAGCGCGCCCAAACCACCGTCAAGGCCATCCAGATCCAGGTCGGCCGCACCGGCACCCT
GACCCCGGTCGCCATTCTTGAACCGGTCACCGTCGGCGGCGTCGTCGTTGGCCGGGCGACGCTGCATAACGAGGACGAGA
TCGCCCGCAAGGATGTGCGCATCGGCGATATGGTCACCATCCAGCGCGCCGGCGATGTCATTCCCCAGGTGGTCGAGGTT
CTGCTCGACCAGCGTCCGGCCGATAGCGCGCCCTTTGTCTTCCCCGAGACCTGCCCGGTCTGCGGAAGCCACGCGGTGCG
CCAAGAGGGCGAGGTCGCCCGGCGCTGCGCCGGCGGGCTGATCTGCTCGGCCCAGGCAGTCGAGCGCCTCAAGCATTTCG
TCTCGCGCGACGCCTTCGATATCGAAGGGCTGGGCGGCAAGCATATCGAGACCTTCCATAATGAGGGCCTGATCACCCAA
CCGGCCGATATTTTCGCGCTGGAAGCCAAGGACAACGAACCCGGCGCCTTGCAGCACCTGCGCTCGCGCGAGGGCTGGGG
GCCGAAATCGGCGGAAAACCTGTTCAAGGCGATCACCGCCCGCAAGACCATCCCCTTGGACCGTTTCATCTACGCCCTGG
GCATCCGTCAGGTTGGTCAGGCCACCGCCCGCCTGCTCGCCGGTCATTACGCCAGCTTCGCCGCCTGGCGCAAGGCGATG
GACGAGGCGCAAACCGAAGGCTCCGAAGCCTTCGCCGATCTTCAAAACATCGAAAGCATCGGCCCCTCGGTCGCCCGCGA
CCTTGTCGCCTTTTTTGGCGAAGAGCATAACCGCGCGGCCTTGGACGCCCTGATCGACGCCGGGGTCGAGGTTCTTGATT
TCGCCCGCCCCCAGGCGGCCGGCGACAGCCCGCTGGCCGGCAAGACCGTGGTTTTCACCGGCACCCTTGATACCATGAGC
CGGGGCGAAGCCAAGGCCCGCGCCCTGGCGCTCGGCGCCAAGGTCACCGGCTCGGTCTCGGCCAAGACCGATTACGTGGT
GGTCGGAGCGGACGCCGGATCCAAGGAGAAGAAGGCCCGCGAACTCGGCCTGACCGTCCTCAGCGAGGCCGAGTTCCGGG
CGATGAGCGGTGGCTGA

Upstream 100 bases:

>100_bases
GAGCGGACCGGGTACGCGCGCCGCCAAAGCACCGCCCTCCGCCCCCCGCTCGTCCTGACCCCGCCCCCCCGTCGCCCCAG
ACCAAACCGGACCCCGCGCC

Downstream 100 bases:

>100_bases
CCGGCAAGGGGCGGAGGATGTGACATCGCCTTCGCCTGTTCGCATCTTTGCGATAAAAAGACCCTACCCTTAGGTGTAAG
ATCACCGCCTTCGCCCGCGG

Product: DNA ligase (NAD+)

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MIPVADLTEAEAARELERLAAEIARHDRLYHQQDAPEITDGEYDALVRRNNEIEAAFPELVRGDSPSAHVGAAPAEGFAK
VTHALPMLSLGNVFSDDEAREFDQRIRRFLSLDGETTISYVAEPKIDGLSFSARYENGVYRRAATRGDGTTGEDITANLA
TLTDLPQRLTGDGPVPEVLEVRGEVFMTKADFRALNQRQIDAGAKPFANPRNAAAGSLRQLDPEITRSRSLSLFAYASGE
VKGLEVESHHAFLERLKTWGFPVNPQTRPCQGVEDLIEATHALALQRAALDYDIDGVVYKVDRVDWQKRLGFVSRAPRWA
IARKFPAERAQTTVKAIQIQVGRTGTLTPVAILEPVTVGGVVVGRATLHNEDEIARKDVRIGDMVTIQRAGDVIPQVVEV
LLDQRPADSAPFVFPETCPVCGSHAVRQEGEVARRCAGGLICSAQAVERLKHFVSRDAFDIEGLGGKHIETFHNEGLITQ
PADIFALEAKDNEPGALQHLRSREGWGPKSAENLFKAITARKTIPLDRFIYALGIRQVGQATARLLAGHYASFAAWRKAM
DEAQTEGSEAFADLQNIESIGPSVARDLVAFFGEEHNRAALDALIDAGVEVLDFARPQAAGDSPLAGKTVVFTGTLDTMS
RGEAKARALALGAKVTGSVSAKTDYVVVGADAGSKEKKARELGLTVLSEAEFRAMSGG

Sequences:

>Translated_698_residues
MIPVADLTEAEAARELERLAAEIARHDRLYHQQDAPEITDGEYDALVRRNNEIEAAFPELVRGDSPSAHVGAAPAEGFAK
VTHALPMLSLGNVFSDDEAREFDQRIRRFLSLDGETTISYVAEPKIDGLSFSARYENGVYRRAATRGDGTTGEDITANLA
TLTDLPQRLTGDGPVPEVLEVRGEVFMTKADFRALNQRQIDAGAKPFANPRNAAAGSLRQLDPEITRSRSLSLFAYASGE
VKGLEVESHHAFLERLKTWGFPVNPQTRPCQGVEDLIEATHALALQRAALDYDIDGVVYKVDRVDWQKRLGFVSRAPRWA
IARKFPAERAQTTVKAIQIQVGRTGTLTPVAILEPVTVGGVVVGRATLHNEDEIARKDVRIGDMVTIQRAGDVIPQVVEV
LLDQRPADSAPFVFPETCPVCGSHAVRQEGEVARRCAGGLICSAQAVERLKHFVSRDAFDIEGLGGKHIETFHNEGLITQ
PADIFALEAKDNEPGALQHLRSREGWGPKSAENLFKAITARKTIPLDRFIYALGIRQVGQATARLLAGHYASFAAWRKAM
DEAQTEGSEAFADLQNIESIGPSVARDLVAFFGEEHNRAALDALIDAGVEVLDFARPQAAGDSPLAGKTVVFTGTLDTMS
RGEAKARALALGAKVTGSVSAKTDYVVVGADAGSKEKKARELGLTVLSEAEFRAMSGG
>Mature_698_residues
MIPVADLTEAEAARELERLAAEIARHDRLYHQQDAPEITDGEYDALVRRNNEIEAAFPELVRGDSPSAHVGAAPAEGFAK
VTHALPMLSLGNVFSDDEAREFDQRIRRFLSLDGETTISYVAEPKIDGLSFSARYENGVYRRAATRGDGTTGEDITANLA
TLTDLPQRLTGDGPVPEVLEVRGEVFMTKADFRALNQRQIDAGAKPFANPRNAAAGSLRQLDPEITRSRSLSLFAYASGE
VKGLEVESHHAFLERLKTWGFPVNPQTRPCQGVEDLIEATHALALQRAALDYDIDGVVYKVDRVDWQKRLGFVSRAPRWA
IARKFPAERAQTTVKAIQIQVGRTGTLTPVAILEPVTVGGVVVGRATLHNEDEIARKDVRIGDMVTIQRAGDVIPQVVEV
LLDQRPADSAPFVFPETCPVCGSHAVRQEGEVARRCAGGLICSAQAVERLKHFVSRDAFDIEGLGGKHIETFHNEGLITQ
PADIFALEAKDNEPGALQHLRSREGWGPKSAENLFKAITARKTIPLDRFIYALGIRQVGQATARLLAGHYASFAAWRKAM
DEAQTEGSEAFADLQNIESIGPSVARDLVAFFGEEHNRAALDALIDAGVEVLDFARPQAAGDSPLAGKTVVFTGTLDTMS
RGEAKARALALGAKVTGSVSAKTDYVVVGADAGSKEKKARELGLTVLSEAEFRAMSGG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=689, Percent_Identity=48.1857764876633, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI87082305, Length=538, Percent_Identity=24.5353159851301, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_RHORT (Q2RVV5)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426027.1
- ProteinModelPortal:   Q2RVV5
- STRING:   Q2RVV5
- GeneID:   3834396
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0939
- NMPDR:   fig|1085.1.peg.3433
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q2RVV5
- ProtClustDB:   CLSK745212
- BioCyc:   RRUB269796:RRU_A0939-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75626; Mature: 75626

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 125-125 BINDING 123-123 BINDING 146-146 BINDING 184-184 BINDING 300-300 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVADLTEAEAARELERLAAEIARHDRLYHQQDAPEITDGEYDALVRRNNEIEAAFPEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHH
VRGDSPSAHVGAAPAEGFAKVTHALPMLSLGNVFSDDEAREFDQRIRRFLSLDGETTISY
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VAEPKIDGLSFSARYENGVYRRAATRGDGTTGEDITANLATLTDLPQRLTGDGPVPEVLE
EECCCCCCCEEEEHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
VRGEVFMTKADFRALNQRQIDAGAKPFANPRNAAAGSLRQLDPEITRSRSLSLFAYASGE
CCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHCHHHHCCHHHHHCCCEEEEEEECCC
VKGLEVESHHAFLERLKTWGFPVNPQTRPCQGVEDLIEATHALALQRAALDYDIDGVVYK
EEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VDRVDWQKRLGFVSRAPRWAIARKFPAERAQTTVKAIQIQVGRTGTLTPVAILEPVTVGG
ECHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHEEEEECCCCCCCHHHHHCCCEECC
VVVGRATLHNEDEIARKDVRIGDMVTIQRAGDVIPQVVEVLLDQRPADSAPFVFPETCPV
EEEEHHHCCCCHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCC
CGSHAVRQEGEVARRCAGGLICSAQAVERLKHFVSRDAFDIEGLGGKHIETFHNEGLITQ
CCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCEEC
PADIFALEAKDNEPGALQHLRSREGWGPKSAENLFKAITARKTIPLDRFIYALGIRQVGQ
CCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ATARLLAGHYASFAAWRKAMDEAQTEGSEAFADLQNIESIGPSVARDLVAFFGEEHNRAA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHH
LDALIDAGVEVLDFARPQAAGDSPLAGKTVVFTGTLDTMSRGEAKARALALGAKVTGSVS
HHHHHHCCHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHHCCHHHHHHHHCCCCCCCCCC
AKTDYVVVGADAGSKEKKARELGLTVLSEAEFRAMSGG
CCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MIPVADLTEAEAARELERLAAEIARHDRLYHQQDAPEITDGEYDALVRRNNEIEAAFPEL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHH
VRGDSPSAHVGAAPAEGFAKVTHALPMLSLGNVFSDDEAREFDQRIRRFLSLDGETTISY
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VAEPKIDGLSFSARYENGVYRRAATRGDGTTGEDITANLATLTDLPQRLTGDGPVPEVLE
EECCCCCCCEEEEHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
VRGEVFMTKADFRALNQRQIDAGAKPFANPRNAAAGSLRQLDPEITRSRSLSLFAYASGE
CCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHCHHHHCCHHHHHCCCEEEEEEECCC
VKGLEVESHHAFLERLKTWGFPVNPQTRPCQGVEDLIEATHALALQRAALDYDIDGVVYK
EEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VDRVDWQKRLGFVSRAPRWAIARKFPAERAQTTVKAIQIQVGRTGTLTPVAILEPVTVGG
ECHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHEEEEECCCCCCCHHHHHCCCEECC
VVVGRATLHNEDEIARKDVRIGDMVTIQRAGDVIPQVVEVLLDQRPADSAPFVFPETCPV
EEEEHHHCCCCHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCC
CGSHAVRQEGEVARRCAGGLICSAQAVERLKHFVSRDAFDIEGLGGKHIETFHNEGLITQ
CCCHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCEEC
PADIFALEAKDNEPGALQHLRSREGWGPKSAENLFKAITARKTIPLDRFIYALGIRQVGQ
CCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ATARLLAGHYASFAAWRKAMDEAQTEGSEAFADLQNIESIGPSVARDLVAFFGEEHNRAA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHH
LDALIDAGVEVLDFARPQAAGDSPLAGKTVVFTGTLDTMSRGEAKARALALGAKVTGSVS
HHHHHHCCHHHHHCCCCCCCCCCCCCCCEEEEEECHHHHHCCHHHHHHHHCCCCCCCCCC
AKTDYVVVGADAGSKEKKARELGLTVLSEAEFRAMSGG
CCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA