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The map label for this gene is pbuE [H]

Identifier: 83592136

GI number: 83592136

Start: 951704

End: 952939

Strand: Reverse

Name: pbuE [H]

Synonym: Rru_A0797

Alternate gene names: 83592136

Gene position: 952939-951704 (Counterclockwise)

Preceding gene: 83592137

Following gene: 83592135

Centisome position: 21.89

GC content: 68.37

Gene sequence:

>1236_bases
ATGACCTCTCTTTCCCATACCGAGCCCGTGAACAAACCGCTGCTCTGGGTCCTTGGGCTTGGCACCTTCGCCATCGGCAC
CGATGCCTTCATCGTCGCCGGCGTTCTACGCGAGATCGCCGACGACTTCGCGGTGTCGGGAGCCGCGGCGGGGCAGTTGA
TCACCCTTTTCGCCGTCTCCTACGCCGTGTTCGCCCCCGTCAGCGCCTGGCTTCTCGGCGGATGGGGGCGGCGACGATCG
CTGCTTTTCGCCCTCGGCGTCTTCGCGCTCGGCAATATCGTCTCGGCGGCGGCGCCCTCTTTCGCCCTCCTCGCCCTCAG
CCGGATCATCGCCGCCTTCGGCGCGGCCAGCTACACCCCCCAGGCGGTGGCCGTCGCCGCGCAGCTTGTGCCGCCCGAGC
GCAAGGGCTGGGCGTTGTCCATCGTGTATGGCGGCATGACGGTGGCGACGGCGCTGGGCATTCCCTTTGGCACCCTGATC
GGCCAGACCCTGGGTTGGCGGGCGACGTTCATCGGCGTCGTCGGCCTTGGCCTGCTGACCCTGGCCGGTCTGGCCGTGCT
GCTGCCCGCCCTGGCCGCGCCGGGCATCCATACCTTGCGCGAGCGCTTCGCCGCCGTTTCCCGCCCCGCCGTTCTCGCGC
CCCTTGGCATCACCTTCCTGGCGGTGCTGTCGGAACATACGGTCTACAGCTACATCAGCCTGATCCTGGCCGACACCCTG
TGGGACGGCCTGCCGGTTCTTCCCATCGCCCTGGTGTTTTTCGGGATCGGCGCGGTGGCCGGCAACGCCGCGTCGGGATA
TGGCAGCGATCGGTTTGGCACCCGCAGCGTGCTGATCTTCGCCATCAGCGCGCAGACGGCGGCCCTTCTTGGCGTCAGCC
TCGTTCAGCCGTCGCCGCTGCTGTCGTGCCTTGTCTTGCTGGTCTGGGGGATGGCCGGTTGGATGTATTTGGTGCCGATC
CAGCACCGGCTGCTCGGGCTGTCCAAAACCTATGGCAGCTTCACGGTGTCGCTCAACAGCTCGGTGCTCTACCTGGGCAT
CGGCGGCGGCGGCGCCCTGGGCGGGCTGTTCATCACGCTTTTTGGCCCGGCCAGTCTCGGCTTCCTTGGCTTCGCCCTCG
GCCTCCTCGCCCTGGCGCTCGCCCTCACCGCCTATGAGGCGGGCGATCGCGACCAGCCCCCCTCGCCGCCCCTGGCCAAT
GAGCCAGTCCCCCTGTCGCCGGCAAAGGATCGATGA

Upstream 100 bases:

>100_bases
GTCACACCGACCGCCGCCCATAGCGGGGACGACGTGAAGGCCTTGCTGTCGGCCTTCGACAAAGTCTGGAACACGGTGAC
ACCAGGAGTCGCATGAAACC

Downstream 100 bases:

>100_bases
TGAGCCCTCCTCGCCCCTTTCAGGCGCAGGCGGCGATATCGGTGGCTTCGATGGGATCAACCATGGATCGGTCCTTTGCG
AAGCGCGGCGTTCGGGTCGT

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTSLSHTEPVNKPLLWVLGLGTFAIGTDAFIVAGVLREIADDFAVSGAAAGQLITLFAVSYAVFAPVSAWLLGGWGRRRS
LLFALGVFALGNIVSAAAPSFALLALSRIIAAFGAASYTPQAVAVAAQLVPPERKGWALSIVYGGMTVATALGIPFGTLI
GQTLGWRATFIGVVGLGLLTLAGLAVLLPALAAPGIHTLRERFAAVSRPAVLAPLGITFLAVLSEHTVYSYISLILADTL
WDGLPVLPIALVFFGIGAVAGNAASGYGSDRFGTRSVLIFAISAQTAALLGVSLVQPSPLLSCLVLLVWGMAGWMYLVPI
QHRLLGLSKTYGSFTVSLNSSVLYLGIGGGGALGGLFITLFGPASLGFLGFALGLLALALALTAYEAGDRDQPPSPPLAN
EPVPLSPAKDR

Sequences:

>Translated_411_residues
MTSLSHTEPVNKPLLWVLGLGTFAIGTDAFIVAGVLREIADDFAVSGAAAGQLITLFAVSYAVFAPVSAWLLGGWGRRRS
LLFALGVFALGNIVSAAAPSFALLALSRIIAAFGAASYTPQAVAVAAQLVPPERKGWALSIVYGGMTVATALGIPFGTLI
GQTLGWRATFIGVVGLGLLTLAGLAVLLPALAAPGIHTLRERFAAVSRPAVLAPLGITFLAVLSEHTVYSYISLILADTL
WDGLPVLPIALVFFGIGAVAGNAASGYGSDRFGTRSVLIFAISAQTAALLGVSLVQPSPLLSCLVLLVWGMAGWMYLVPI
QHRLLGLSKTYGSFTVSLNSSVLYLGIGGGGALGGLFITLFGPASLGFLGFALGLLALALALTAYEAGDRDQPPSPPLAN
EPVPLSPAKDR
>Mature_410_residues
TSLSHTEPVNKPLLWVLGLGTFAIGTDAFIVAGVLREIADDFAVSGAAAGQLITLFAVSYAVFAPVSAWLLGGWGRRRSL
LFALGVFALGNIVSAAAPSFALLALSRIIAAFGAASYTPQAVAVAAQLVPPERKGWALSIVYGGMTVATALGIPFGTLIG
QTLGWRATFIGVVGLGLLTLAGLAVLLPALAAPGIHTLRERFAAVSRPAVLAPLGITFLAVLSEHTVYSYISLILADTLW
DGLPVLPIALVFFGIGAVAGNAASGYGSDRFGTRSVLIFAISAQTAALLGVSLVQPSPLLSCLVLLVWGMAGWMYLVPIQ
HRLLGLSKTYGSFTVSLNSSVLYLGIGGGGALGGLFITLFGPASLGFLGFALGLLALALALTAYEAGDRDQPPSPPLANE
PVPLSPAKDR

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=331, Percent_Identity=32.6283987915408, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786595, Length=261, Percent_Identity=27.5862068965517, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787808, Length=233, Percent_Identity=28.755364806867, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693198, Length=338, Percent_Identity=25.7396449704142, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323735, Length=261, Percent_Identity=27.2030651340996, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42193; Mature: 42062

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSHTEPVNKPLLWVLGLGTFAIGTDAFIVAGVLREIADDFAVSGAAAGQLITLFAVS
CCCCCCCCCCCCCEEHEEEHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YAVFAPVSAWLLGGWGRRRSLLFALGVFALGNIVSAAAPSFALLALSRIIAAFGAASYTP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
QAVAVAAQLVPPERKGWALSIVYGGMTVATALGIPFGTLIGQTLGWRATFIGVVGLGLLT
HHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHH
LAGLAVLLPALAAPGIHTLRERFAAVSRPAVLAPLGITFLAVLSEHTVYSYISLILADTL
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WDGLPVLPIALVFFGIGAVAGNAASGYGSDRFGTRSVLIFAISAQTAALLGVSLVQPSPL
HCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCHH
LSCLVLLVWGMAGWMYLVPIQHRLLGLSKTYGSFTVSLNSSVLYLGIGGGGALGGLFITL
HHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHH
FGPASLGFLGFALGLLALALALTAYEAGDRDQPPSPPLANEPVPLSPAKDR
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSLSHTEPVNKPLLWVLGLGTFAIGTDAFIVAGVLREIADDFAVSGAAAGQLITLFAVS
CCCCCCCCCCCCEEHEEEHHHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
YAVFAPVSAWLLGGWGRRRSLLFALGVFALGNIVSAAAPSFALLALSRIIAAFGAASYTP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
QAVAVAAQLVPPERKGWALSIVYGGMTVATALGIPFGTLIGQTLGWRATFIGVVGLGLLT
HHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHH
LAGLAVLLPALAAPGIHTLRERFAAVSRPAVLAPLGITFLAVLSEHTVYSYISLILADTL
HHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WDGLPVLPIALVFFGIGAVAGNAASGYGSDRFGTRSVLIFAISAQTAALLGVSLVQPSPL
HCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHCCCCHH
LSCLVLLVWGMAGWMYLVPIQHRLLGLSKTYGSFTVSLNSSVLYLGIGGGGALGGLFITL
HHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCHHHHHHHHHH
FGPASLGFLGFALGLLALALALTAYEAGDRDQPPSPPLANEPVPLSPAKDR
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]