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The map label for this gene is aes [H]

Identifier: 83592037

GI number: 83592037

Start: 827771

End: 828802

Strand: Reverse

Name: aes [H]

Synonym: Rru_A0698

Alternate gene names: 83592037

Gene position: 828802-827771 (Counterclockwise)

Preceding gene: 83592038

Following gene: 83592033

Centisome position: 19.04

GC content: 70.74

Gene sequence:

>1032_bases
ATGTCCGATCCGTTGACCCTGTTCACGCGGGGCGGTTCCAGTCTCCCCACCATCGCTCGGCCGACGTCCACCCTGGCCGG
ACTGCATCCCGAAGCCGCCGCGCTGTTTGACTCCCTTGAGCGCTCGGGAATCTTGTCGGGGCGGCCGGAAAGTCAGCCCC
TGGCCACGGCGCGCGCCCGGGCCCAATCGTTCTGGTATCCCTGGAACACCGGCGGTCCGCGCATGCGGCTGCGCCGCGAC
CTCAGCTTGGACGGGTCCGATGGCCCGGTGGCGGCCCGGCTGTTCATTCCCTGTTCCGCCAGCAGCGAACCGCCGGTGAT
CGTCTATTTCCACGGCGGCGGCTATGCCCTCAACGGCCTGGATACCCATGATCGGCTGATGCGGACCCTGGCCGACGCAT
CGGGCGCGGCGGTGCTTGGCGTCGGCTATGCCCTGGCGCCCGAGCATCCCTTCCCCACCCAAACCGCCCAGGCGCTGTCG
GCCATCGACTGGCTGTTGCGCCGGGGACGTCGCGACTTCGCCATCGATGCCCGGCGCATGGCCCTGGCCGGCGACTCGGC
CGGCGCCCACCTCGCCCTGTGGACGGCCCAGTCGATGCGCGAAGCCGATTGGCCGGCCGCCCGCATGCTGCTGTTGGCCT
ATGGCATGATGGTGCCGCGCTTCGACAGCGCCTCCCATGCCCTTTATGGCGGCGGCGCCTATGGCCTGACCACCGAACGC
ATGCGCTGGTTCTGGAACGCGGTGACCCCGGCCCAGGGAAGGCCCTTCACGCCGCTGACCGCCGGGCTCGACGATCTGCC
GTCCTGTCTGGTGGTCGGCGCCAGCGCCGATTGCCTGCGCGATGACAGCCGCGCCCTGGCCGAAGCCCTGGAGCTGCGCG
ACGCGCCGGTCCGCTTCATCGAAGCCGAAGGCCTACCCCATTCCTTCTTGCAATACGCCCCGGCGCTCGAGCCGGCGCGC
CGCCTGCTGGGCGATCTCGCCTCCACGCTGGCGCGCAGCCTGGAAAGCGGCGCCCCGGTCCGCTGGTCCTAG

Upstream 100 bases:

>100_bases
CGGAAATCGGACGCAGAGGGGTTGCCATGCCGAACACGCCTGTCCTTGGCCGCCTTGGATCCCCCAGCCCTTGCGGGCAC
CCCAAAGGGGAGGAACGCTG

Downstream 100 bases:

>100_bases
AGCAAATCCCGAGCAATCTGGACCAGATTGCGACGACGATTTGCTTAACTATCAATATCTTAGAGCAGAGAGCGGGGATC
CTGTTTCACGCTCTCTGTTC

Product: lipolytic protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSDPLTLFTRGGSSLPTIARPTSTLAGLHPEAAALFDSLERSGILSGRPESQPLATARARAQSFWYPWNTGGPRMRLRRD
LSLDGSDGPVAARLFIPCSASSEPPVIVYFHGGGYALNGLDTHDRLMRTLADASGAAVLGVGYALAPEHPFPTQTAQALS
AIDWLLRRGRRDFAIDARRMALAGDSAGAHLALWTAQSMREADWPAARMLLLAYGMMVPRFDSASHALYGGGAYGLTTER
MRWFWNAVTPAQGRPFTPLTAGLDDLPSCLVVGASADCLRDDSRALAEALELRDAPVRFIEAEGLPHSFLQYAPALEPAR
RLLGDLASTLARSLESGAPVRWS

Sequences:

>Translated_343_residues
MSDPLTLFTRGGSSLPTIARPTSTLAGLHPEAAALFDSLERSGILSGRPESQPLATARARAQSFWYPWNTGGPRMRLRRD
LSLDGSDGPVAARLFIPCSASSEPPVIVYFHGGGYALNGLDTHDRLMRTLADASGAAVLGVGYALAPEHPFPTQTAQALS
AIDWLLRRGRRDFAIDARRMALAGDSAGAHLALWTAQSMREADWPAARMLLLAYGMMVPRFDSASHALYGGGAYGLTTER
MRWFWNAVTPAQGRPFTPLTAGLDDLPSCLVVGASADCLRDDSRALAEALELRDAPVRFIEAEGLPHSFLQYAPALEPAR
RLLGDLASTLARSLESGAPVRWS
>Mature_342_residues
SDPLTLFTRGGSSLPTIARPTSTLAGLHPEAAALFDSLERSGILSGRPESQPLATARARAQSFWYPWNTGGPRMRLRRDL
SLDGSDGPVAARLFIPCSASSEPPVIVYFHGGGYALNGLDTHDRLMRTLADASGAAVLGVGYALAPEHPFPTQTAQALSA
IDWLLRRGRRDFAIDARRMALAGDSAGAHLALWTAQSMREADWPAARMLLLAYGMMVPRFDSASHALYGGGAYGLTTERM
RWFWNAVTPAQGRPFTPLTAGLDDLPSCLVVGASADCLRDDSRALAEALELRDAPVRFIEAEGLPHSFLQYAPALEPARR
LLGDLASTLARSLESGAPVRWS

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=122, Percent_Identity=33.6065573770492, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157041239, Length=120, Percent_Identity=32.5, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786682, Length=274, Percent_Identity=33.9416058394161, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI72001146, Length=154, Percent_Identity=32.4675324675325, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24656084, Length=111, Percent_Identity=35.1351351351351, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24656076, Length=111, Percent_Identity=35.1351351351351, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20130169, Length=111, Percent_Identity=35.1351351351351, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 36868; Mature: 36737

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPLTLFTRGGSSLPTIARPTSTLAGLHPEAAALFDSLERSGILSGRPESQPLATARAR
CCCCEEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AQSFWYPWNTGGPRMRLRRDLSLDGSDGPVAARLFIPCSASSEPPVIVYFHGGGYALNGL
HHHCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCEEECCC
DTHDRLMRTLADASGAAVLGVGYALAPEHPFPTQTAQALSAIDWLLRRGRRDFAIDARRM
CHHHHHHHHHHCCCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEECHHHH
ALAGDSAGAHLALWTAQSMREADWPAARMLLLAYGMMVPRFDSASHALYGGGAYGLTTER
EEECCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHH
MRWFWNAVTPAQGRPFTPLTAGLDDLPSCLVVGASADCLRDDSRALAEALELRDAPVRFI
HHHHHHCCCCCCCCCCCHHHCCHHHCCCCEEECCCHHHHCCHHHHHHHHHHHCCCCEEEE
EAEGLPHSFLQYAPALEPARRLLGDLASTLARSLESGAPVRWS
ECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDPLTLFTRGGSSLPTIARPTSTLAGLHPEAAALFDSLERSGILSGRPESQPLATARAR
CCCEEEEECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AQSFWYPWNTGGPRMRLRRDLSLDGSDGPVAARLFIPCSASSEPPVIVYFHGGGYALNGL
HHHCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCEEECCC
DTHDRLMRTLADASGAAVLGVGYALAPEHPFPTQTAQALSAIDWLLRRGRRDFAIDARRM
CHHHHHHHHHHCCCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEECHHHH
ALAGDSAGAHLALWTAQSMREADWPAARMLLLAYGMMVPRFDSASHALYGGGAYGLTTER
EEECCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHH
MRWFWNAVTPAQGRPFTPLTAGLDDLPSCLVVGASADCLRDDSRALAEALELRDAPVRFI
HHHHHHCCCCCCCCCCCHHHCCHHHCCCCEEECCCHHHHCCHHHHHHHHHHHCCCCEEEE
EAEGLPHSFLQYAPALEPARRLLGDLASTLARSLESGAPVRWS
ECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA