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The map label for this gene is norM [H]

Identifier: 83592036

GI number: 83592036

Start: 826246

End: 827652

Strand: Direct

Name: norM [H]

Synonym: Rru_A0697

Alternate gene names: 83592036

Gene position: 826246-827652 (Clockwise)

Preceding gene: 83592035

Following gene: 83592040

Centisome position: 18.98

GC content: 69.37

Gene sequence:

>1407_bases
GTGATTTCCTCATCGGTTTCCTTGCCACGGTCGGGCCATGGAGCATGGCGGACCGAATTGGGCGCCACCTTGTCGCTCGC
TTGGCCGCTGATCCTGACCAATCTGGCCCAGGCGGCGCTGACCACCACCGATCTTTTGCTGATCGGCAGCCTGGGGTCGC
GGTCCCTGGCCGCCGCCGCCATGGCCGCCGGCCTGTTTCAGGCGCTGATGCTGTTCGGCCTGGGGCTGGTCTCGGCGACC
TTGCCGCTGCTGGCCACGGCTTTGGGCCGCAACCGTCATAGCGTGCGCGATGTGCGGCGCACCGTGCGCCAGGGCCTATG
GTCGGCGGGGCTGATCAGCCTGCCGCTGTGGCTGATCCTTTGGCATACGGAGGCGGTGCTGATCGCCTTTGGTCAGGAGC
CCGCCCTGGCGGCCGAGGCGGCCGGTTTCATGCATACCCTGCAATGGGGGCTGCTGCCCTTCCTCGGCTATATCGTGCTG
CGCGCCTTTCTTTCGGCGTTGGAACGGCCGATGTGGCCCTTGCTGGTCGCCGGATTGGCCATCGGCTTCAACGCCCTGGC
GGCCTGGGCGCTGATCTTCGGCCACCTTGGCCTGCCGGCCCTGGGGCTGCCCGGGGCCGGGGTGTCGAGCAGCCTGTCGA
GCCTGTTCATGGTTGCCGGGCTGGCCCTGGTCATCACCCGCCAGCGCCGCCTGAAGCGCTACCGGCTGTTTGGCCGCTTC
TGGCGGCCCGATTGGCCACGTCTGGTCACCCTGTGGCGCCTGGGTTTGCCGATGGCGATCAGTTTCGCCTTCGAGGTGTC
GATCTTCCAGGCCTCGATGATGATGATGGGCATTCTGGGGGCGGACGCCCTGGCCGCCCATACCATCGCCCTGCAAATCG
CCACGCTGACCTTCATGGTGCCCCTGGGGCTGGGGCAGGCGGCGACGGTGCGGGTCGGGCGGGCGCTGGGAGCGGGCACC
ATTCCGGCCCTGCGCCGCGCCGGCTGGGTGGCCTATGGCGCCGGCGTCGCCTTCATGGGCGGGGCGGCCCTGATGATGCT
CAGCGTGCCCCGGTTGCTGATCGGCGCTTTCCTGGATGCCGATCTGGCCGAAAACGCCCCGGTGATCGATCTGGCGGTGG
TGCTGATGGCCCTGGCCGCGCTGTTCCAGATCGCCGATGGCGCCCAGGCGGTGTGCAACGGCATGCTGCGCGGCCTGCAT
GACACCAAGGTGCCGATGCTGCTGGCCGCCCTGGGCTATTGGGGCTTGGGCCTGCCCTTGGGCGCCTTGCTCGCCTTCCC
TTTGGGGGTGGGCGCGCCCGGCGTGTGGATCGGGCTGGCCGGCGGGCTTTCGGTGGTGGCCGTGCTGATGACCCGGCGCT
GGATCCGTCTGGTCGCCGCCCTGGAACGCGCCAGCGTGACCGCTTAG

Upstream 100 bases:

>100_bases
TGGTCAAGGAATTGCGCCACGGCCGGGTCGAACGCGCCCAACCGCTGCCGGTTGGCGTTGTCCAACCGCTGTCGGTTGGC
GTTGTGGAAGATGGTGCGGG

Downstream 100 bases:

>100_bases
GCGGTCGCTCCGGGGGTAGAACAGAGAGCGTGAAACAGGATCCCCGCTCTCTGCTCTAAGATATTGATAGTTAAGCAAAT
CGTCGTCGCAATCTGGTCCA

Product: multi anti extrusion protein MatE

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MISSSVSLPRSGHGAWRTELGATLSLAWPLILTNLAQAALTTTDLLLIGSLGSRSLAAAAMAAGLFQALMLFGLGLVSAT
LPLLATALGRNRHSVRDVRRTVRQGLWSAGLISLPLWLILWHTEAVLIAFGQEPALAAEAAGFMHTLQWGLLPFLGYIVL
RAFLSALERPMWPLLVAGLAIGFNALAAWALIFGHLGLPALGLPGAGVSSSLSSLFMVAGLALVITRQRRLKRYRLFGRF
WRPDWPRLVTLWRLGLPMAISFAFEVSIFQASMMMMGILGADALAAHTIALQIATLTFMVPLGLGQAATVRVGRALGAGT
IPALRRAGWVAYGAGVAFMGGAALMMLSVPRLLIGAFLDADLAENAPVIDLAVVLMALAALFQIADGAQAVCNGMLRGLH
DTKVPMLLAALGYWGLGLPLGALLAFPLGVGAPGVWIGLAGGLSVVAVLMTRRWIRLVAALERASVTA

Sequences:

>Translated_468_residues
MISSSVSLPRSGHGAWRTELGATLSLAWPLILTNLAQAALTTTDLLLIGSLGSRSLAAAAMAAGLFQALMLFGLGLVSAT
LPLLATALGRNRHSVRDVRRTVRQGLWSAGLISLPLWLILWHTEAVLIAFGQEPALAAEAAGFMHTLQWGLLPFLGYIVL
RAFLSALERPMWPLLVAGLAIGFNALAAWALIFGHLGLPALGLPGAGVSSSLSSLFMVAGLALVITRQRRLKRYRLFGRF
WRPDWPRLVTLWRLGLPMAISFAFEVSIFQASMMMMGILGADALAAHTIALQIATLTFMVPLGLGQAATVRVGRALGAGT
IPALRRAGWVAYGAGVAFMGGAALMMLSVPRLLIGAFLDADLAENAPVIDLAVVLMALAALFQIADGAQAVCNGMLRGLH
DTKVPMLLAALGYWGLGLPLGALLAFPLGVGAPGVWIGLAGGLSVVAVLMTRRWIRLVAALERASVTA
>Mature_468_residues
MISSSVSLPRSGHGAWRTELGATLSLAWPLILTNLAQAALTTTDLLLIGSLGSRSLAAAAMAAGLFQALMLFGLGLVSAT
LPLLATALGRNRHSVRDVRRTVRQGLWSAGLISLPLWLILWHTEAVLIAFGQEPALAAEAAGFMHTLQWGLLPFLGYIVL
RAFLSALERPMWPLLVAGLAIGFNALAAWALIFGHLGLPALGLPGAGVSSSLSSLFMVAGLALVITRQRRLKRYRLFGRF
WRPDWPRLVTLWRLGLPMAISFAFEVSIFQASMMMMGILGADALAAHTIALQIATLTFMVPLGLGQAATVRVGRALGAGT
IPALRRAGWVAYGAGVAFMGGAALMMLSVPRLLIGAFLDADLAENAPVIDLAVVLMALAALFQIADGAQAVCNGMLRGLH
DTKVPMLLAALGYWGLGLPLGALLAFPLGVGAPGVWIGLAGGLSVVAVLMTRRWIRLVAALERASVTA

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=434, Percent_Identity=26.7281105990783, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI153792564, Length=429, Percent_Identity=25.4079254079254, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI38261960, Length=464, Percent_Identity=23.7068965517241, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI48994890, Length=452, Percent_Identity=32.9646017699115, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI226510954, Length=443, Percent_Identity=25.0564334085779, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320545, Length=436, Percent_Identity=22.7064220183486, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49235; Mature: 49235

Theoretical pI: Translated: 12.00; Mature: 12.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSSVSLPRSGHGAWRTELGATLSLAWPLILTNLAQAALTTTDLLLIGSLGSRSLAAAA
CCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHH
MAAGLFQALMLFGLGLVSATLPLLATALGRNRHSVRDVRRTVRQGLWSAGLISLPLWLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
WHTEAVLIAFGQEPALAAEAAGFMHTLQWGLLPFLGYIVLRAFLSALERPMWPLLVAGLA
HHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IGFNALAAWALIFGHLGLPALGLPGAGVSSSLSSLFMVAGLALVITRQRRLKRYRLFGRF
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRPDWPRLVTLWRLGLPMAISFAFEVSIFQASMMMMGILGADALAAHTIALQIATLTFMV
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
PLGLGQAATVRVGRALGAGTIPALRRAGWVAYGAGVAFMGGAALMMLSVPRLLIGAFLDA
HCCCCCHHHHHHHHHHCCCCHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLAENAPVIDLAVVLMALAALFQIADGAQAVCNGMLRGLHDTKVPMLLAALGYWGLGLPL
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GALLAFPLGVGAPGVWIGLAGGLSVVAVLMTRRWIRLVAALERASVTA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISSSVSLPRSGHGAWRTELGATLSLAWPLILTNLAQAALTTTDLLLIGSLGSRSLAAAA
CCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHH
MAAGLFQALMLFGLGLVSATLPLLATALGRNRHSVRDVRRTVRQGLWSAGLISLPLWLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
WHTEAVLIAFGQEPALAAEAAGFMHTLQWGLLPFLGYIVLRAFLSALERPMWPLLVAGLA
HHHCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
IGFNALAAWALIFGHLGLPALGLPGAGVSSSLSSLFMVAGLALVITRQRRLKRYRLFGRF
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WRPDWPRLVTLWRLGLPMAISFAFEVSIFQASMMMMGILGADALAAHTIALQIATLTFMV
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
PLGLGQAATVRVGRALGAGTIPALRRAGWVAYGAGVAFMGGAALMMLSVPRLLIGAFLDA
HCCCCCHHHHHHHHHHCCCCHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLAENAPVIDLAVVLMALAALFQIADGAQAVCNGMLRGLHDTKVPMLLAALGYWGLGLPL
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
GALLAFPLGVGAPGVWIGLAGGLSVVAVLMTRRWIRLVAALERASVTA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]