Click here to switch to the map view.

The map label for this gene is folD

Identifier: 83591896

GI number: 83591896

Start: 658159

End: 659049

Strand: Direct

Name: folD

Synonym: Rru_A0557

Alternate gene names: 83591896

Gene position: 658159-659049 (Clockwise)

Preceding gene: 83591890

Following gene: 83591899

Centisome position: 15.12

GC content: 66.78

Gene sequence:

>891_bases
ATGGGTGCGCATTTGATCGATGGTAAGGCCATCGCCCAGCAGCTTCGCGAGCGCATCGCCCAGGAGGTCGCCGATTTGAA
GGCCCATCGCGGCTTGGTGCCCGGCTTGGCGGTGGTGCTGGTCGGCGAGGATGCCGCCAGTCAGGTCTATGTCGGCTCGA
AAGAGAAGATGGCCGTCGCCGTCGGCATGCATTCGGTGGTCTGCCGCCTGGCGGCCACGACCTCGGAGGCCGAGGTGCTG
GCCAAAGTGGAGGCGCTCAACGCCGATCCGACGATCGACGGTATCTTGGTGCAATTGCCGCTGCCCGCCCATATCGACGA
GGACAAGATCCTGCTCGCCATCGATCCCGACAAGGATGTCGATGGCTTCCATCCGGTCAATGTCGGGCGGCTGTCGATCG
GCCAGTCGAATGGCGAGCACGGCATGGTGCCCTGCACCCCCAAGGGCTGCCTGATGCTGCTCAAGGGCGTGCTCGGCGAT
TTGACCGGCAAGACCGCCCTTGTCATCGGCCGCTCCAATATCGTGGGCAAGCCGATGGCCCAATTGCTGCTGTCGGCCAA
TTGCACCGTCACCCTCGCCCATTCGCGCAGCCGCGATCTGCCCGATCTCTGCCGGCGCATGGATATCGTCGTCGCCGCCG
TCGGCCGGCCCGAGATGGTCAAGGGCGACTGGCTGAAGCCGGGCGCCGTGGTGATCGATGTCGGGATGAACCGCTTGCCC
GCGGCCCCGGGGGCCGAGAAGGGGAAGTTGGTCGGCGATGTCGATTATGCCTCGGCCGCCGCCGTGGCCGGGGCGATCAC
CCCGGTGCCGGGCGGCGTTGGGCCGATGACCATCGCCTGTTTGCTGGCCAATACCCTGGAGGCCTGCAAGGCGCGGGGCG
CCGGCGCCTGA

Upstream 100 bases:

>100_bases
TGCGGGGTGGACTTTGCGCGGCTTTACCGGGCCCGACTTGCGGAGTATCGTGCGCCCCTTTCCGAAAATGGGCCGTTTTG
GCCGAGCAGGGAGACCGCCG

Downstream 100 bases:

>100_bases
GAGCCCGGCTCCGCTCACAGCATACGGGCGAAGTCCTCGGGCGGCAGCGGCCGGCCGAACAGATAACCCTGGATCAGATC
GCAATAGTGCTCGCGCAGAA

Product: methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MGAHLIDGKAIAQQLRERIAQEVADLKAHRGLVPGLAVVLVGEDAASQVYVGSKEKMAVAVGMHSVVCRLAATTSEAEVL
AKVEALNADPTIDGILVQLPLPAHIDEDKILLAIDPDKDVDGFHPVNVGRLSIGQSNGEHGMVPCTPKGCLMLLKGVLGD
LTGKTALVIGRSNIVGKPMAQLLLSANCTVTLAHSRSRDLPDLCRRMDIVVAAVGRPEMVKGDWLKPGAVVIDVGMNRLP
AAPGAEKGKLVGDVDYASAAAVAGAITPVPGGVGPMTIACLLANTLEACKARGAGA

Sequences:

>Translated_296_residues
MGAHLIDGKAIAQQLRERIAQEVADLKAHRGLVPGLAVVLVGEDAASQVYVGSKEKMAVAVGMHSVVCRLAATTSEAEVL
AKVEALNADPTIDGILVQLPLPAHIDEDKILLAIDPDKDVDGFHPVNVGRLSIGQSNGEHGMVPCTPKGCLMLLKGVLGD
LTGKTALVIGRSNIVGKPMAQLLLSANCTVTLAHSRSRDLPDLCRRMDIVVAAVGRPEMVKGDWLKPGAVVIDVGMNRLP
AAPGAEKGKLVGDVDYASAAAVAGAITPVPGGVGPMTIACLLANTLEACKARGAGA
>Mature_295_residues
GAHLIDGKAIAQQLRERIAQEVADLKAHRGLVPGLAVVLVGEDAASQVYVGSKEKMAVAVGMHSVVCRLAATTSEAEVLA
KVEALNADPTIDGILVQLPLPAHIDEDKILLAIDPDKDVDGFHPVNVGRLSIGQSNGEHGMVPCTPKGCLMLLKGVLGDL
TGKTALVIGRSNIVGKPMAQLLLSANCTVTLAHSRSRDLPDLCRRMDIVVAAVGRPEMVKGDWLKPGAVVIDVGMNRLPA
APGAEKGKLVGDVDYASAAAVAGAITPVPGGVGPMTIACLLANTLEACKARGAGA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=45.0171821305842, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=44.2567567567568, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=41.2162162162162, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI36796743, Length=212, Percent_Identity=26.8867924528302, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786741, Length=289, Percent_Identity=53.9792387543253, Blast_Score=281, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=301, Percent_Identity=41.8604651162791, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=46.6887417218543, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=44.8275862068966, Blast_Score=252, Evalue=5e-68,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=48.6206896551724, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=48.6206896551724, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=48.6206896551724, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=48.6206896551724, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI17136816, Length=300, Percent_Identity=45, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17136818, Length=300, Percent_Identity=45, Blast_Score=234, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_RHORT (Q2RWY4)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_425648.1
- ProteinModelPortal:   Q2RWY4
- SMR:   Q2RWY4
- STRING:   Q2RWY4
- GeneID:   3834235
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0557
- NMPDR:   fig|1085.1.peg.1783
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- PhylomeDB:   Q2RWY4
- BioCyc:   RRUB269796:RRU_A0557-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30448; Mature: 30317

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAHLIDGKAIAQQLRERIAQEVADLKAHRGLVPGLAVVLVGEDAASQVYVGSKEKMAVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCEEEE
VGMHSVVCRLAATTSEAEVLAKVEALNADPTIDGILVQLPLPAHIDEDKILLAIDPDKDV
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCC
DGFHPVNVGRLSIGQSNGEHGMVPCTPKGCLMLLKGVLGDLTGKTALVIGRSNIVGKPMA
CCCCCCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
QLLLSANCTVTLAHSRSRDLPDLCRRMDIVVAAVGRPEMVKGDWLKPGAVVIDVGMNRLP
HHHHCCCCEEEEECCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEECCCCCCC
AAPGAEKGKLVGDVDYASAAAVAGAITPVPGGVGPMTIACLLANTLEACKARGAGA
CCCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GAHLIDGKAIAQQLRERIAQEVADLKAHRGLVPGLAVVLVGEDAASQVYVGSKEKMAVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCEEEE
VGMHSVVCRLAATTSEAEVLAKVEALNADPTIDGILVQLPLPAHIDEDKILLAIDPDKDV
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCC
DGFHPVNVGRLSIGQSNGEHGMVPCTPKGCLMLLKGVLGDLTGKTALVIGRSNIVGKPMA
CCCCCCCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
QLLLSANCTVTLAHSRSRDLPDLCRRMDIVVAAVGRPEMVKGDWLKPGAVVIDVGMNRLP
HHHHCCCCEEEEECCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCEEEECCCCCCC
AAPGAEKGKLVGDVDYASAAAVAGAITPVPGGVGPMTIACLLANTLEACKARGAGA
CCCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA