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The map label for this gene is cheB1

Identifier: 83591862

GI number: 83591862

Start: 617675

End: 618838

Strand: Direct

Name: cheB1

Synonym: Rru_A0523

Alternate gene names: 83591862

Gene position: 617675-618838 (Clockwise)

Preceding gene: 83591861

Following gene: 83591863

Centisome position: 14.19

GC content: 66.15

Gene sequence:

>1164_bases
GTGACGATAACGACGTCCGAAGCGACCAGCCATACCCTCGGCGGACCGATCCGGGTCATGGTCGTTGATGATTCCGCCGT
GGTGCGGGGGCTGGAAACCCGCATGCTCGAAGAGGATCCGGCCATCCAGGTCGTCGCTTCGGTGGGCAATGGTCAGATGG
CGGTGCAAGCGCTCGACCGGCACGACATCGAGGTGGTGATCCTCGATATCGAGATGCCAGTGATGGACGGGTTGACCGCC
TTGCCCGAGTTATTGCGAAAGTCGCCCAATCTCAAGGTGATCATGGCCTCGACCCTGACCCTGCGCAACGCCGAGGTCAC
GCTCAAGGCCCTGCAGATGGGAGCCTCGGAATGTCTGGCCAAGCCGACGACGTCGCGCGAGATTTCGGGGGGGACGGATT
TCCGGCACGATCTGGTGGAGAAGGTCAAGGCTTTGGGCGGGGCGCGGCGCCGCGCCCTGGGACGGGCGGCGCCGACGGCG
CGGGCCGGCGGGGCCGTGGTCGAGCGCAAGGTCGGGGCTCTGCCCTCGCTGATGGCCCAGCGCGCCCAGCAGCCGATCTC
CCTGCGTCCGGCGGCCGAGGAGCGCCCCGATATCATCGCCATCGGCAGTTCGACCGGCGGTCCCCAGGCGCTGTTCACGG
TTTTTGGCGATATGCGCAAGGGCGGTTGGCCCAGTCAGCCGATCGTCGTCACCCAGCATATGCCCGCGACCTTCACCACC
ATTCTGGCCGGACATATCGAACGGGTGGCGGGGGTGCCGACGGCCGAGGCCAAGGATGGCGATCCCATTCGCGGCGGCCA
TATCTACATCGCGCCGGGCGACTATCACATGGTCGTGGAAACCCGGGGGACGGAAAAAATCCTGCGATTGAACCAGGACC
CACCGGAAAGCTTTTGTCGTCCCGCCGTCGATCCGCTGTTCCGCTCCGTCGCCAAGGCCTATGGCCGGCGGGTGCTGGCA
GTGGTGCTGACCGGCATGGGCGCCGACGGCTCCAAGGGAGGCAAGATCATCGCAGAGTCTGGGGGCACCGTGATCGCCCA
GGACGAGCCCAGCAGTGTGGTGTGGGGAATGCCCGGAGCGACGGCCCAGATCGGGGCCTGTTCCGCCGTTCTTCCCCTCA
AGGACATTGCGGCCTACGTTCTCCGATCCGCGAACAAGCGCTGA

Upstream 100 bases:

>100_bases
ATTATGAAACCTTTCGACAGCGAGATCCTTCAGGCCAAATTCCAGCAGGTCGGTCTTCTGGACGCATGAGCGTTTTCGGA
ACAGTGGGAAAGAGCAAAGC

Downstream 100 bases:

>100_bases
TGGCATGAAACCGGAAGATTTCGACTTCATCTCCAAGCTGCTGAAAGATAAATCCGGGCTCGTTCTGACCAAGGACAAGT
CCTACCTTATCGAAAGCCGC

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTITTSEATSHTLGGPIRVMVVDDSAVVRGLETRMLEEDPAIQVVASVGNGQMAVQALDRHDIEVVILDIEMPVMDGLTA
LPELLRKSPNLKVIMASTLTLRNAEVTLKALQMGASECLAKPTTSREISGGTDFRHDLVEKVKALGGARRRALGRAAPTA
RAGGAVVERKVGALPSLMAQRAQQPISLRPAAEERPDIIAIGSSTGGPQALFTVFGDMRKGGWPSQPIVVTQHMPATFTT
ILAGHIERVAGVPTAEAKDGDPIRGGHIYIAPGDYHMVVETRGTEKILRLNQDPPESFCRPAVDPLFRSVAKAYGRRVLA
VVLTGMGADGSKGGKIIAESGGTVIAQDEPSSVVWGMPGATAQIGACSAVLPLKDIAAYVLRSANKR

Sequences:

>Translated_387_residues
MTITTSEATSHTLGGPIRVMVVDDSAVVRGLETRMLEEDPAIQVVASVGNGQMAVQALDRHDIEVVILDIEMPVMDGLTA
LPELLRKSPNLKVIMASTLTLRNAEVTLKALQMGASECLAKPTTSREISGGTDFRHDLVEKVKALGGARRRALGRAAPTA
RAGGAVVERKVGALPSLMAQRAQQPISLRPAAEERPDIIAIGSSTGGPQALFTVFGDMRKGGWPSQPIVVTQHMPATFTT
ILAGHIERVAGVPTAEAKDGDPIRGGHIYIAPGDYHMVVETRGTEKILRLNQDPPESFCRPAVDPLFRSVAKAYGRRVLA
VVLTGMGADGSKGGKIIAESGGTVIAQDEPSSVVWGMPGATAQIGACSAVLPLKDIAAYVLRSANKR
>Mature_386_residues
TITTSEATSHTLGGPIRVMVVDDSAVVRGLETRMLEEDPAIQVVASVGNGQMAVQALDRHDIEVVILDIEMPVMDGLTAL
PELLRKSPNLKVIMASTLTLRNAEVTLKALQMGASECLAKPTTSREISGGTDFRHDLVEKVKALGGARRRALGRAAPTAR
AGGAVVERKVGALPSLMAQRAQQPISLRPAAEERPDIIAIGSSTGGPQALFTVFGDMRKGGWPSQPIVVTQHMPATFTTI
LAGHIERVAGVPTAEAKDGDPIRGGHIYIAPGDYHMVVETRGTEKILRLNQDPPESFCRPAVDPLFRSVAKAYGRRVLAV
VLTGMGADGSKGGKIIAESGGTVIAQDEPSSVVWGMPGATAQIGACSAVLPLKDIAAYVLRSANKR

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=367, Percent_Identity=34.3324250681199, Blast_Score=188, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_RHORT (Q2RX18)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_425614.1
- ProteinModelPortal:   Q2RX18
- SMR:   Q2RX18
- STRING:   Q2RX18
- GeneID:   3834491
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0523
- NMPDR:   fig|1085.1.peg.1815
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- PhylomeDB:   Q2RX18
- ProtClustDB:   PRK00742
- BioCyc:   RRUB269796:RRU_A0523-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 40794; Mature: 40663

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 204-204 ACT_SITE 233-233 ACT_SITE 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITTSEATSHTLGGPIRVMVVDDSAVVRGLETRMLEEDPAIQVVASVGNGQMAVQALDR
CEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCC
HDIEVVILDIEMPVMDGLTALPELLRKSPNLKVIMASTLTLRNAEVTLKALQMGASECLA
CCEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHHHCHHHHHC
KPTTSREISGGTDFRHDLVEKVKALGGARRRALGRAAPTARAGGAVVERKVGALPSLMAQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHH
RAQQPISLRPAAEERPDIIAIGSSTGGPQALFTVFGDMRKGGWPSQPIVVTQHMPATFTT
HHCCCCEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHH
ILAGHIERVAGVPTAEAKDGDPIRGGHIYIAPGDYHMVVETRGTEKILRLNQDPPESFCR
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHCCCCCCHHHHHH
PAVDPLFRSVAKAYGRRVLAVVLTGMGADGSKGGKIIAESGGTVIAQDEPSSVVWGMPGA
HHHHHHHHHHHHHHHHHEEEHHEECCCCCCCCCCEEEEECCCEEEECCCCCCEEECCCCC
TAQIGACSAVLPLKDIAAYVLRSANKR
CCCCCCCHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TITTSEATSHTLGGPIRVMVVDDSAVVRGLETRMLEEDPAIQVVASVGNGQMAVQALDR
EEECCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCC
HDIEVVILDIEMPVMDGLTALPELLRKSPNLKVIMASTLTLRNAEVTLKALQMGASECLA
CCEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHHHCHHHHHC
KPTTSREISGGTDFRHDLVEKVKALGGARRRALGRAAPTARAGGAVVERKVGALPSLMAQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHH
RAQQPISLRPAAEERPDIIAIGSSTGGPQALFTVFGDMRKGGWPSQPIVVTQHMPATFTT
HHCCCCEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHH
ILAGHIERVAGVPTAEAKDGDPIRGGHIYIAPGDYHMVVETRGTEKILRLNQDPPESFCR
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHCCCCCCHHHHHH
PAVDPLFRSVAKAYGRRVLAVVLTGMGADGSKGGKIIAESGGTVIAQDEPSSVVWGMPGA
HHHHHHHHHHHHHHHHHEEEHHEECCCCCCCCCCEEEEECCCEEEECCCCCCEEECCCCC
TAQIGACSAVLPLKDIAAYVLRSANKR
CCCCCCCHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA