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The map label for this gene is hyfI [H]

Identifier: 83591661

GI number: 83591661

Start: 391105

End: 391626

Strand: Direct

Name: hyfI [H]

Synonym: Rru_A0321

Alternate gene names: 83591661

Gene position: 391105-391626 (Clockwise)

Preceding gene: 83591660

Following gene: 83591662

Centisome position: 8.99

GC content: 66.67

Gene sequence:

>522_bases
ATGTCCCTTTATGATTTCATCATGCGCAAGGTCTTCAAGCGCTCGCTGTGGGTCTTTCATGCGAACGCCGGAGCTTGCAA
CGGCTGCGACATCGAAGAGGTCAACGCCTTCACCCCCTATTACGACGCCGAGCGCTTCGGCCTGAAGCTGGTTGGCTCGC
CCCGCCACGCCGATGTGCTGCTGATCTCGGGGCCGGTCACCCGCACCGCCGAACCCCGATTGCGCCGGCTTTATGAGCAG
ACCCCCAACCCCAAGGTGGTGATCGCCTGCGGGTCCTGCGCCGTGGGCGGCGGCATGTGGTTTGACAGCTACCACGTGTT
GGGCGGCGCCGACAAAGTGGTGCCCGTCGACTTCTACATCCCCGGCTGTCCGCCCCGCCCCGAAGCCATCCTGTGGGGGG
TGGCGGTGGCCATGGGGTTGGTCGAGGCCAAGGCCCAGCCCTACAGCTACCGCCAGGAACACGCCCTGCCGATCCCCGTG
CGCAAGCCGGCGGCCGAGCCCCCGCGCGAAACGGCCGAGTAG

Upstream 100 bases:

>100_bases
GTTCTGGTCGTCGTCACCGGTCTCAACCTCAGTGTCTTCAACCGTCTCATCGACCTATCGGTCGGCGTGCTGCTGCGCTA
GCGCGCCCGGAGAAACGGCC

Downstream 100 bases:

>100_bases
GGGCGGGCCATGACGATCTTCGATGACCTGGGCGGGCGCCTGAAGGGCCGGGTTCTGGTCGTGGGAGCGGGCAACCCCTT
GCGCGGTGATGACGGCGCCG

Product: NADH dehydrogenase (ubiquinone), 20 kDa subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSLYDFIMRKVFKRSLWVFHANAGACNGCDIEEVNAFTPYYDAERFGLKLVGSPRHADVLLISGPVTRTAEPRLRRLYEQ
TPNPKVVIACGSCAVGGGMWFDSYHVLGGADKVVPVDFYIPGCPPRPEAILWGVAVAMGLVEAKAQPYSYRQEHALPIPV
RKPAAEPPRETAE

Sequences:

>Translated_173_residues
MSLYDFIMRKVFKRSLWVFHANAGACNGCDIEEVNAFTPYYDAERFGLKLVGSPRHADVLLISGPVTRTAEPRLRRLYEQ
TPNPKVVIACGSCAVGGGMWFDSYHVLGGADKVVPVDFYIPGCPPRPEAILWGVAVAMGLVEAKAQPYSYRQEHALPIPV
RKPAAEPPRETAE
>Mature_172_residues
SLYDFIMRKVFKRSLWVFHANAGACNGCDIEEVNAFTPYYDAERFGLKLVGSPRHADVLLISGPVTRTAEPRLRRLYEQT
PNPKVVIACGSCAVGGGMWFDSYHVLGGADKVVPVDFYIPGCPPRPEAILWGVAVAMGLVEAKAQPYSYRQEHALPIPVR
KPAAEPPRETAE

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=122, Percent_Identity=45.0819672131148, Blast_Score=117, Evalue=4e-27,
Organism=Escherichia coli, GI1788834, Length=140, Percent_Identity=49.2857142857143, Blast_Score=153, Evalue=6e-39,
Organism=Escherichia coli, GI1789074, Length=137, Percent_Identity=51.0948905109489, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1788624, Length=139, Percent_Identity=39.568345323741, Blast_Score=108, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17509685, Length=106, Percent_Identity=49.0566037735849, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI18859983, Length=106, Percent_Identity=46.2264150943396, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24642371, Length=106, Percent_Identity=46.2264150943396, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24651058, Length=122, Percent_Identity=45.0819672131148, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 19090; Mature: 18959

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLYDFIMRKVFKRSLWVFHANAGACNGCDIEEVNAFTPYYDAERFGLKLVGSPRHADVL
CCHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHCCCCCCCCCCHHCCEEEECCCCCCEEE
LISGPVTRTAEPRLRRLYEQTPNPKVVIACGSCAVGGGMWFDSYHVLGGADKVVPVDFYI
EEECCCCCCCCHHHHHHHHHCCCCEEEEEECCEECCCCCEECCEEEECCCCCEEEEEEEE
PGCPPRPEAILWGVAVAMGLVEAKAQPYSYRQEHALPIPVRKPAAEPPRETAE
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLYDFIMRKVFKRSLWVFHANAGACNGCDIEEVNAFTPYYDAERFGLKLVGSPRHADVL
CHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHCCCCCCCCCCHHCCEEEECCCCCCEEE
LISGPVTRTAEPRLRRLYEQTPNPKVVIACGSCAVGGGMWFDSYHVLGGADKVVPVDFYI
EEECCCCCCCCHHHHHHHHHCCCCEEEEEECCEECCCCCEECCEEEECCCCCEEEEEEEE
PGCPPRPEAILWGVAVAMGLVEAKAQPYSYRQEHALPIPVRKPAAEPPRETAE
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]