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The map label for this gene is hyfF [H]

Identifier: 83591660

GI number: 83591660

Start: 389490

End: 391085

Strand: Direct

Name: hyfF [H]

Synonym: Rru_A0320

Alternate gene names: 83591660

Gene position: 389490-391085 (Clockwise)

Preceding gene: 83591659

Following gene: 83591661

Centisome position: 8.95

GC content: 64.79

Gene sequence:

>1596_bases
ATGGCGAATTCATCCTTACTACTTCTGTTATTCCTGATTCCCGCCCTGGGGGCGGTCGTGGAATATCCCTTGGGCCGCGC
CCGCTCGGCCCTGGCCGGCGCGCCTTCGGTGGCCGGGGCGCTTGGCGCCTTCGCCGTCGCCGTGACCATGGCCTTCCGTA
TCGCCGAAAGCGGCGACCTCTTCGCCCTGGGCGGCTGGTTCCATGCCGATGCCCTGTCGGCGGTGGTGGCGATCGTCGTC
ACCTTCGTCGGCTTGGCCGCCACCTTGTTCTCGATCGGCTACCTGCGCCACGAGGTCGAGGCCGGGCAGTATTCCCAAAC
GCGGCTGCCGGTCTATTTCGTGCTGAACTCGGTGTTCATCGCCACCATGGCGGCGGTCACCGTCACCAACAACGTCATCA
TGATGTATGTGGTGGTCGAGGCGACGACCCTGGCCTCGGCCCTGCTGGTCACCTACTACCGCAAGCCGGGTGCCTGGGAA
GCGGGCTATAAGTACCTGCTTCTGTGCTCGGTCGGCATCACCATGGGCCTGCTGGGCTGCGTCATTCTGTATTCGGCGGC
GGTGCCGCTGCTCGGCGGCCACCGGGCGATGCAGATCAGCGAACTGGCCACCGTCGCCGACCAATTGCCGCCGACCGCCG
TGGTCGTCGGCTGCGTGCTGATCATCATCGGCTTTGGCTCCAAGGCCGGTCTGATCCCCTTCCACGCCTGGCTGCCCGAC
GCCCATTCCCAATCGCCAAGCCCGGTCAGCGCCCTGCTGTCGGGGGTGACCCTGAAGGTCGCCATTTACGCCATCGCCCG
CATCGCCAGCCTGTTCTATGCCGGTCACGGTGCTTTGGGCGTCTTTTGCATCGTCCTTGGCGCGATCACCATGCTGGGCG
GCATCATCGCCGCCTTCAGCCAGACCGACTTGAAACGCCTGCTCGCCTACAGCTCGGTCAGTCAGGTCGGCTATATCATC
ATGGGCCTGGGCATCGGCTCGTACCTGGGCTTTTACGGCGCGGTTTATCACCTGATGAACCACGCCCTTGATAAGGCCAT
GCTGTTCCTGTGCACCGGCCTGCTGCTCTATTCCTGCGGCACCACCTCGATCGAGGAATTGGGCAAGCGCAAGCATTCGC
CGCTGGTCGCCCTCTGCTTCTTCATCGGCGCCCTGGCGATCAGCGGCGTGCCGCCGCTCAACGGCTTCTGGAGCAAATTC
GCCATCTACACCGCCGCCGCCGAGGCCCATCTGTGGTGGGCTCTGGGCGTGGCGCTGCTCACCAGCCTGATCACCATCGC
CGTGCTGGTGCGCGCGGGCTATCTGATCTTCCTGCGCAACACCGACCACGACCACGGTTCCACCGACGTTTCCGGCGACG
CCGCCCTGGCGTTTGGCGATGGCGCCGCAACTCCGACTTTTGACGGCTCCGCCGGCACCCCGGCTTTTGACGGCTCCGCC
GTCCTGGCTGTGGGCGGTCGGGTTCCCCACCAGGGCTATCCGGTGATGATGATCGGCGTCGTTGTCACGATGACCGTTCT
GGTCGTCGTCACCGGTCTCAACCTCAGTGTCTTCAACCGTCTCATCGACCTATCGGTCGGCGTGCTGCTGCGCTAG

Upstream 100 bases:

>100_bases
GTGTGGATGATGCTTTACGTCGTCCGCTCGATCTACGGCGCCGTCGGCTCCACCGACACCACCAATATTTCGCAGCTGAA
TGGCTGAGGGAGCCGGGACA

Downstream 100 bases:

>100_bases
CGCGCCCGGAGAAACGGCCATGTCCCTTTATGATTTCATCATGCGCAAGGTCTTCAAGCGCTCGCTGTGGGTCTTTCATG
CGAACGCCGGAGCTTGCAAC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MANSSLLLLLFLIPALGAVVEYPLGRARSALAGAPSVAGALGAFAVAVTMAFRIAESGDLFALGGWFHADALSAVVAIVV
TFVGLAATLFSIGYLRHEVEAGQYSQTRLPVYFVLNSVFIATMAAVTVTNNVIMMYVVVEATTLASALLVTYYRKPGAWE
AGYKYLLLCSVGITMGLLGCVILYSAAVPLLGGHRAMQISELATVADQLPPTAVVVGCVLIIIGFGSKAGLIPFHAWLPD
AHSQSPSPVSALLSGVTLKVAIYAIARIASLFYAGHGALGVFCIVLGAITMLGGIIAAFSQTDLKRLLAYSSVSQVGYII
MGLGIGSYLGFYGAVYHLMNHALDKAMLFLCTGLLLYSCGTTSIEELGKRKHSPLVALCFFIGALAISGVPPLNGFWSKF
AIYTAAAEAHLWWALGVALLTSLITIAVLVRAGYLIFLRNTDHDHGSTDVSGDAALAFGDGAATPTFDGSAGTPAFDGSA
VLAVGGRVPHQGYPVMMIGVVVTMTVLVVVTGLNLSVFNRLIDLSVGVLLR

Sequences:

>Translated_531_residues
MANSSLLLLLFLIPALGAVVEYPLGRARSALAGAPSVAGALGAFAVAVTMAFRIAESGDLFALGGWFHADALSAVVAIVV
TFVGLAATLFSIGYLRHEVEAGQYSQTRLPVYFVLNSVFIATMAAVTVTNNVIMMYVVVEATTLASALLVTYYRKPGAWE
AGYKYLLLCSVGITMGLLGCVILYSAAVPLLGGHRAMQISELATVADQLPPTAVVVGCVLIIIGFGSKAGLIPFHAWLPD
AHSQSPSPVSALLSGVTLKVAIYAIARIASLFYAGHGALGVFCIVLGAITMLGGIIAAFSQTDLKRLLAYSSVSQVGYII
MGLGIGSYLGFYGAVYHLMNHALDKAMLFLCTGLLLYSCGTTSIEELGKRKHSPLVALCFFIGALAISGVPPLNGFWSKF
AIYTAAAEAHLWWALGVALLTSLITIAVLVRAGYLIFLRNTDHDHGSTDVSGDAALAFGDGAATPTFDGSAGTPAFDGSA
VLAVGGRVPHQGYPVMMIGVVVTMTVLVVVTGLNLSVFNRLIDLSVGVLLR
>Mature_530_residues
ANSSLLLLLFLIPALGAVVEYPLGRARSALAGAPSVAGALGAFAVAVTMAFRIAESGDLFALGGWFHADALSAVVAIVVT
FVGLAATLFSIGYLRHEVEAGQYSQTRLPVYFVLNSVFIATMAAVTVTNNVIMMYVVVEATTLASALLVTYYRKPGAWEA
GYKYLLLCSVGITMGLLGCVILYSAAVPLLGGHRAMQISELATVADQLPPTAVVVGCVLIIIGFGSKAGLIPFHAWLPDA
HSQSPSPVSALLSGVTLKVAIYAIARIASLFYAGHGALGVFCIVLGAITMLGGIIAAFSQTDLKRLLAYSSVSQVGYIIM
GLGIGSYLGFYGAVYHLMNHALDKAMLFLCTGLLLYSCGTTSIEELGKRKHSPLVALCFFIGALAISGVPPLNGFWSKFA
IYTAAAEAHLWWALGVALLTSLITIAVLVRAGYLIFLRNTDHDHGSTDVSGDAALAFGDGAATPTFDGSAGTPAFDGSAV
LAVGGRVPHQGYPVMMIGVVVTMTVLVVVTGLNLSVFNRLIDLSVGVLLR

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=375, Percent_Identity=29.0666666666667, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI251831116, Length=271, Percent_Identity=26.9372693726937, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788831, Length=358, Percent_Identity=40.5027932960894, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788827, Length=352, Percent_Identity=32.9545454545455, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1788829, Length=406, Percent_Identity=33.0049261083744, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1788614, Length=348, Percent_Identity=34.7701149425287, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI2367154, Length=346, Percent_Identity=30.3468208092486, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788613, Length=185, Percent_Identity=37.2972972972973, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI145693160, Length=375, Percent_Identity=27.7333333333333, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 55348; Mature: 55217

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSSLLLLLFLIPALGAVVEYPLGRARSALAGAPSVAGALGAFAVAVTMAFRIAESGDL
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
FALGGWFHADALSAVVAIVVTFVGLAATLFSIGYLRHEVEAGQYSQTRLPVYFVLNSVFI
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ATMAAVTVTNNVIMMYVVVEATTLASALLVTYYRKPGAWEAGYKYLLLCSVGITMGLLGC
HHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHH
VILYSAAVPLLGGHRAMQISELATVADQLPPTAVVVGCVLIIIGFGSKAGLIPFHAWLPD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEHHHCCC
AHSQSPSPVSALLSGVTLKVAIYAIARIASLFYAGHGALGVFCIVLGAITMLGGIIAAFS
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QTDLKRLLAYSSVSQVGYIIMGLGIGSYLGFYGAVYHLMNHALDKAMLFLCTGLLLYSCG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TTSIEELGKRKHSPLVALCFFIGALAISGVPPLNGFWSKFAIYTAAAEAHLWWALGVALL
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TSLITIAVLVRAGYLIFLRNTDHDHGSTDVSGDAALAFGDGAATPTFDGSAGTPAFDGSA
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCE
VLAVGGRVPHQGYPVMMIGVVVTMTVLVVVTGLNLSVFNRLIDLSVGVLLR
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANSSLLLLLFLIPALGAVVEYPLGRARSALAGAPSVAGALGAFAVAVTMAFRIAESGDL
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
FALGGWFHADALSAVVAIVVTFVGLAATLFSIGYLRHEVEAGQYSQTRLPVYFVLNSVFI
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ATMAAVTVTNNVIMMYVVVEATTLASALLVTYYRKPGAWEAGYKYLLLCSVGITMGLLGC
HHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHH
VILYSAAVPLLGGHRAMQISELATVADQLPPTAVVVGCVLIIIGFGSKAGLIPFHAWLPD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEHHHCCC
AHSQSPSPVSALLSGVTLKVAIYAIARIASLFYAGHGALGVFCIVLGAITMLGGIIAAFS
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
QTDLKRLLAYSSVSQVGYIIMGLGIGSYLGFYGAVYHLMNHALDKAMLFLCTGLLLYSCG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TTSIEELGKRKHSPLVALCFFIGALAISGVPPLNGFWSKFAIYTAAAEAHLWWALGVALL
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TSLITIAVLVRAGYLIFLRNTDHDHGSTDVSGDAALAFGDGAATPTFDGSAGTPAFDGSA
HHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCE
VLAVGGRVPHQGYPVMMIGVVVTMTVLVVVTGLNLSVFNRLIDLSVGVLLR
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]