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The map label for this gene is hyfD [H]

Identifier: 83591654

GI number: 83591654

Start: 382129

End: 383646

Strand: Direct

Name: hyfD [H]

Synonym: Rru_A0314

Alternate gene names: 83591654

Gene position: 382129-383646 (Clockwise)

Preceding gene: 83591653

Following gene: 83591655

Centisome position: 8.78

GC content: 65.55

Gene sequence:

>1518_bases
ATGCTTGATGATGTTGTGACCCGGGTCCTGCCCGCGGGCGGCGCCGCCGTCCCTGGTTCGGGCCATGGGCCGGGCGCCGT
GCCCCGCTGGAACGGGGCGGGCCATCGGCCGGCTTCCCCCCGGGCCGGTGCCAATGGCGTGATTTTCCTGGCCTCGCTTA
TCTTCTCGGGGCTGGCCCTGGCCGGCGCTATCGCCGCCATTCTGGCCGTTTTCCCCGGTCCCTGGACCCTCGGCCTGGGG
CGTCTGCCCTGGCTTGCCAGCGATCAGGCCCTGTTCGGGCTGGCGCTTGATCCGCTGTCGTGCCTGATGCTGGTGGTGAT
CACGCTCTGCGGCTTCCTGGTGGTGCTCTACTCGGGCGGTTACATGAGCCTGGGCAACGTCGAGCACCCCTCCGACGCCA
ATCTGGCGCGCTATTACTTCTTTGTTTGCCTGTTCATCACGGCGATGGTCGGGCTGGTGTCGTCGCCCAATTACTTCCAG
CTTTTCCTGTTCTGGGAACTGACGACGCTGTGTTCCTGGGGGCTGATCTCGTTCTATGGCGATGCCGAGGCCCTGAAGTC
GGCCTATAAGGCGCTGCTCATCACCCATGGCGCCGGGCTGTTCCTCGCCGCGGCCGTGGTGCTGATCTATGTCGAGACCG
GCTCTTTCGCGTTCGACGCGATCAATCTGCTGACCCCGGGCCTGAAAAGTCTGGTGATCGCCTTCATGGGCATCGCCGCC
CTGGCCAAGGCCGCCCAATTCCCCTTCTTCACCTGGCTGCCCACCGCCATGGCCGCCCCGACCCCGGCCAGTTCCTATCT
CCACGCCGCGGCGATGGTGAAGGCCGGCATCTATCTGCTGGCGCGGCTGGCGCTCAGCGTCGACGCCTTCCCGCCCGAAC
TCGGGCTGGCGCTCAGCGTCATCGCCATCGTCACCATGTGTCTGGCCGTCGTCCTTTATTTCCCGCAAGATGATCTGAAG
CGGCTGCTCGCCTTCTCGACCATCGCCAATCTTGGCTACATGGTTCTGGGCATCGCCATGGGCGCCATGGGCTCGCGGAC
GGCGCTGGAAGGCGGGATGCTTCATCTCGTTGGCCATTCCTTCACCAAAAGCCTGCTGTTCCTCGCCGTCGGCGCCATTT
CCTACGCCACCGGCACCCGCAAGATCGGCCTGCTCAGCGGTCTGGGCCGCAAGATGCCGGTCACCGCGCTGGCCTTCGTG
CTGGGCGCCATGGCGATCAGCGGCATGCCGCCCTTCAACATGTTCTGGTCCAAATACCTGATCGTTTCGGGCGCCATCGA
GCTCAGCTCGACCTGGGGCTGGACGCTGGCCGTCCTCACCCTGGCCGAGAGCGTCGCCGGCTTCGCCTGGTTCTTGCACA
TCGTCCACAAGGTCTTCTTCGGCCCCGTCTCCCCGACCGCCGAGAATGCGCGCGACCCCGGCGCGGCCATGCTGATCCCC
CTCATCGTCCTGATGGCCTTCAGCGTGCTCTCCACCGTTTTCGCGCTGCCCATGATCCAACAGTCGTTCGGGGGGTGA

Upstream 100 bases:

>100_bases
GGCTTTGTCTGCGACGCCGACACCCTGGCCCGCCTGCTGCGGACCGCCACCGGGATCCCCGCCACCGCTTCGACCATCCT
GTCCATCGGGGGACACCAAA

Downstream 100 bases:

>100_bases
GATCATCATGAGCACAACGCAAGTTTTCCTCGGCGCCTTCGCCGTCCTCGCCGCCCTTCCGGTCCTCACCCTGCTGGCCG
GAAACCGCAAGTCCCTGGCG

Product: hydrogenase 4 subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MLDDVVTRVLPAGGAAVPGSGHGPGAVPRWNGAGHRPASPRAGANGVIFLASLIFSGLALAGAIAAILAVFPGPWTLGLG
RLPWLASDQALFGLALDPLSCLMLVVITLCGFLVVLYSGGYMSLGNVEHPSDANLARYYFFVCLFITAMVGLVSSPNYFQ
LFLFWELTTLCSWGLISFYGDAEALKSAYKALLITHGAGLFLAAAVVLIYVETGSFAFDAINLLTPGLKSLVIAFMGIAA
LAKAAQFPFFTWLPTAMAAPTPASSYLHAAAMVKAGIYLLARLALSVDAFPPELGLALSVIAIVTMCLAVVLYFPQDDLK
RLLAFSTIANLGYMVLGIAMGAMGSRTALEGGMLHLVGHSFTKSLLFLAVGAISYATGTRKIGLLSGLGRKMPVTALAFV
LGAMAISGMPPFNMFWSKYLIVSGAIELSSTWGWTLAVLTLAESVAGFAWFLHIVHKVFFGPVSPTAENARDPGAAMLIP
LIVLMAFSVLSTVFALPMIQQSFGG

Sequences:

>Translated_505_residues
MLDDVVTRVLPAGGAAVPGSGHGPGAVPRWNGAGHRPASPRAGANGVIFLASLIFSGLALAGAIAAILAVFPGPWTLGLG
RLPWLASDQALFGLALDPLSCLMLVVITLCGFLVVLYSGGYMSLGNVEHPSDANLARYYFFVCLFITAMVGLVSSPNYFQ
LFLFWELTTLCSWGLISFYGDAEALKSAYKALLITHGAGLFLAAAVVLIYVETGSFAFDAINLLTPGLKSLVIAFMGIAA
LAKAAQFPFFTWLPTAMAAPTPASSYLHAAAMVKAGIYLLARLALSVDAFPPELGLALSVIAIVTMCLAVVLYFPQDDLK
RLLAFSTIANLGYMVLGIAMGAMGSRTALEGGMLHLVGHSFTKSLLFLAVGAISYATGTRKIGLLSGLGRKMPVTALAFV
LGAMAISGMPPFNMFWSKYLIVSGAIELSSTWGWTLAVLTLAESVAGFAWFLHIVHKVFFGPVSPTAENARDPGAAMLIP
LIVLMAFSVLSTVFALPMIQQSFGG
>Mature_505_residues
MLDDVVTRVLPAGGAAVPGSGHGPGAVPRWNGAGHRPASPRAGANGVIFLASLIFSGLALAGAIAAILAVFPGPWTLGLG
RLPWLASDQALFGLALDPLSCLMLVVITLCGFLVVLYSGGYMSLGNVEHPSDANLARYYFFVCLFITAMVGLVSSPNYFQ
LFLFWELTTLCSWGLISFYGDAEALKSAYKALLITHGAGLFLAAAVVLIYVETGSFAFDAINLLTPGLKSLVIAFMGIAA
LAKAAQFPFFTWLPTAMAAPTPASSYLHAAAMVKAGIYLLARLALSVDAFPPELGLALSVIAIVTMCLAVVLYFPQDDLK
RLLAFSTIANLGYMVLGIAMGAMGSRTALEGGMLHLVGHSFTKSLLFLAVGAISYATGTRKIGLLSGLGRKMPVTALAFV
LGAMAISGMPPFNMFWSKYLIVSGAIELSSTWGWTLAVLTLAESVAGFAWFLHIVHKVFFGPVSPTAENARDPGAAMLIP
LIVLMAFSVLSTVFALPMIQQSFGG

Specific function: Unknown

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=403, Percent_Identity=29.5285359801489, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI251831116, Length=297, Percent_Identity=23.9057239057239, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1788829, Length=419, Percent_Identity=44.3914081145585, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI1788614, Length=419, Percent_Identity=33.890214797136, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1788827, Length=420, Percent_Identity=30.7142857142857, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI1788831, Length=354, Percent_Identity=32.7683615819209, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI2367154, Length=354, Percent_Identity=27.683615819209, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788613, Length=419, Percent_Identity=26.0143198090692, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI145693160, Length=351, Percent_Identity=24.2165242165242, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53005; Mature: 53005

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDVVTRVLPAGGAAVPGSGHGPGAVPRWNGAGHRPASPRAGANGVIFLASLIFSGLAL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGAIAAILAVFPGPWTLGLGRLPWLASDQALFGLALDPLSCLMLVVITLCGFLVVLYSGG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YMSLGNVEHPSDANLARYYFFVCLFITAMVGLVSSPNYFQLFLFWELTTLCSWGLISFYG
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCC
DAEALKSAYKALLITHGAGLFLAAAVVLIYVETGSFAFDAINLLTPGLKSLVIAFMGIAA
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LAKAAQFPFFTWLPTAMAAPTPASSYLHAAAMVKAGIYLLARLALSVDAFPPELGLALSV
HHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IAIVTMCLAVVLYFPQDDLKRLLAFSTIANLGYMVLGIAMGAMGSRTALEGGMLHLVGHS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
FTKSLLFLAVGAISYATGTRKIGLLSGLGRKMPVTALAFVLGAMAISGMPPFNMFWSKYL
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IVSGAIELSSTWGWTLAVLTLAESVAGFAWFLHIVHKVFFGPVSPTAENARDPGAAMLIP
HHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LIVLMAFSVLSTVFALPMIQQSFGG
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDDVVTRVLPAGGAAVPGSGHGPGAVPRWNGAGHRPASPRAGANGVIFLASLIFSGLAL
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AGAIAAILAVFPGPWTLGLGRLPWLASDQALFGLALDPLSCLMLVVITLCGFLVVLYSGG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
YMSLGNVEHPSDANLARYYFFVCLFITAMVGLVSSPNYFQLFLFWELTTLCSWGLISFYG
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCC
DAEALKSAYKALLITHGAGLFLAAAVVLIYVETGSFAFDAINLLTPGLKSLVIAFMGIAA
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
LAKAAQFPFFTWLPTAMAAPTPASSYLHAAAMVKAGIYLLARLALSVDAFPPELGLALSV
HHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
IAIVTMCLAVVLYFPQDDLKRLLAFSTIANLGYMVLGIAMGAMGSRTALEGGMLHLVGHS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHH
FTKSLLFLAVGAISYATGTRKIGLLSGLGRKMPVTALAFVLGAMAISGMPPFNMFWSKYL
HHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IVSGAIELSSTWGWTLAVLTLAESVAGFAWFLHIVHKVFFGPVSPTAENARDPGAAMLIP
HHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
LIVLMAFSVLSTVFALPMIQQSFGG
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]