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The map label for this gene is guaA [C]

Identifier: 83591639

GI number: 83591639

Start: 366429

End: 367151

Strand: Direct

Name: guaA [C]

Synonym: Rru_A0299

Alternate gene names: 83591639

Gene position: 366429-367151 (Clockwise)

Preceding gene: 83591636

Following gene: 83591640

Centisome position: 8.42

GC content: 70.26

Gene sequence:

>723_bases
ATGTCCATCGGTATTCTTGAAACCGGCCGCCCCGCGCCCGCGCTTGCCAGTCGCCATGGCGATTATGTCGAGATGGCCGC
CGGCCTGCTTGCGGCGGGCGCGCCGACGCCCCTGCCCGAGGTCGCCCGCTTCGCCGTGATCGACGGCGAGTTCCCCGACT
CCGCCACCCGTTGTGATGCCTGGGTGGTGACCGGTTCGCGCCATAGCGCCACCGAGGAAGCCCCCTGGATGCTGCGCCTG
GAAGCCCTGCTGCGCGAGGCGGTGGCCGCCGGGCGGCCGGTGATCGGGCTGTGCTTTGGCCATCAGATCCTGGCCAAGGC
CCTGGGCGGACGGGTCGAACCCTCGCCCTTCGGCTGGCAATTGGGCCTTCAGGACTACCGGGTGGTCGATGCCCCCGACT
GGCTGGCCGGTCTAGGGCCGACGATCGGGCTCAACGCCATTCACCAAGACCATGTCAGCGTCGTGCCGCCGGGGGCCCGC
CTGCTGGCCACCGCGCCCCACTGTCCCAATGCCGCCCTGGTCTATGGCAAGGCGGCGATCAGCTTCCAGGGCCACCCCGA
ATTCACCCGCGATTTCGAGCGGGACCTGCTTGCCCTTTATGGCGGCGACACCTTGCCGGCGGACCGCGCCGGCCAAGCCC
TGGCGGCGATGGAGGGCGGCGGCCTGACCGCCGACGCGCTGACCATCGCCGACGCGCTGCGCCGTTTCATCGCAGAGAAA
TAG

Upstream 100 bases:

>100_bases
GCGTTGACGGGGCGCCTTGGTTGGGCGAACCTTGGCCCCTCCCAGCCCCCGACCGGTTTCGCACCCGGGCATCCCCCCAG
CCAAGGCAGTCGTCGTCGCC

Downstream 100 bases:

>100_bases
GCGCTTTTTTTACCCCTCCGACGGATAGAGCAATGAGCACACCGCGTAAGACCTGGACATATTATAAGGGCGATTGGCAC
GAGGGCGATATTCGCATCCT

Product: glutamine amidotransferase class-I

Products: AMP; L-Glutamate; GMP; H+; Diphosphate [C]

Alternate protein names: Glutamine Amidotransferase Class I; Glutamine Amidotransferase; Amidotransferase; GMP Synthase; Class I Glutamine Amidotransferase; GMP Synthase Glutamine Amidotransferase Subunit; Class I Glutamine Amidotransferase Family Protein; Glutamine Amidotransferase Class-I Family Protein; GMP Synthase Subunit A; Glutamine Amidotransferase Class-I Family

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSIGILETGRPAPALASRHGDYVEMAAGLLAAGAPTPLPEVARFAVIDGEFPDSATRCDAWVVTGSRHSATEEAPWMLRL
EALLREAVAAGRPVIGLCFGHQILAKALGGRVEPSPFGWQLGLQDYRVVDAPDWLAGLGPTIGLNAIHQDHVSVVPPGAR
LLATAPHCPNAALVYGKAAISFQGHPEFTRDFERDLLALYGGDTLPADRAGQALAAMEGGGLTADALTIADALRRFIAEK

Sequences:

>Translated_240_residues
MSIGILETGRPAPALASRHGDYVEMAAGLLAAGAPTPLPEVARFAVIDGEFPDSATRCDAWVVTGSRHSATEEAPWMLRL
EALLREAVAAGRPVIGLCFGHQILAKALGGRVEPSPFGWQLGLQDYRVVDAPDWLAGLGPTIGLNAIHQDHVSVVPPGAR
LLATAPHCPNAALVYGKAAISFQGHPEFTRDFERDLLALYGGDTLPADRAGQALAAMEGGGLTADALTIADALRRFIAEK
>Mature_239_residues
SIGILETGRPAPALASRHGDYVEMAAGLLAAGAPTPLPEVARFAVIDGEFPDSATRCDAWVVTGSRHSATEEAPWMLRLE
ALLREAVAAGRPVIGLCFGHQILAKALGGRVEPSPFGWQLGLQDYRVVDAPDWLAGLGPTIGLNAIHQDHVSVVPPGARL
LATAPHCPNAALVYGKAAISFQGHPEFTRDFERDLLALYGGDTLPADRAGQALAAMEGGGLTADALTIADALRRFIAEK

Specific function: Catalyzes The Synthesis Of Gmp From Xmp. [C]

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Saccharomyces cerevisiae, GI6323155, Length=219, Percent_Identity=29.6803652968037, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.5.2 [C]

Molecular weight: Translated: 25200; Mature: 25069

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIGILETGRPAPALASRHGDYVEMAAGLLAAGAPTPLPEVARFAVIDGEFPDSATRCDA
CCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCCCCCCCCE
WVVTGSRHSATEEAPWMLRLEALLREAVAAGRPVIGLCFGHQILAKALGGRVEPSPFGWQ
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCCEE
LGLQDYRVVDAPDWLAGLGPTIGLNAIHQDHVSVVPPGARLLATAPHCPNAALVYGKAAI
ECCCCCEEECCCHHHHHCCCCCCCCCCCCCCCEEECCCHHEEEECCCCCCCEEEEEEEEE
SFQGHPEFTRDFERDLLALYGGDTLPADRAGQALAAMEGGGLTADALTIADALRRFIAEK
EECCCCHHHHHHHHHHHHHHCCCCCCCHHCCCEEEHCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIGILETGRPAPALASRHGDYVEMAAGLLAAGAPTPLPEVARFAVIDGEFPDSATRCDA
CCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHHHHHEEEEECCCCCCCCCCCCE
WVVTGSRHSATEEAPWMLRLEALLREAVAAGRPVIGLCFGHQILAKALGGRVEPSPFGWQ
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCCCCCCCCCEE
LGLQDYRVVDAPDWLAGLGPTIGLNAIHQDHVSVVPPGARLLATAPHCPNAALVYGKAAI
ECCCCCEEECCCHHHHHCCCCCCCCCCCCCCCEEECCCHHEEEECCCCCCCEEEEEEEEE
SFQGHPEFTRDFERDLLALYGGDTLPADRAGQALAAMEGGGLTADALTIADALRRFIAEK
EECCCCHHHHHHHHHHHHHHCCCCCCCHHCCCEEEHCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 2 {1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine} 0.058 {XMP}} 0.029 {XMP}} 1 {NH4+}} 0.42 {6-thioXMP}} 0.35 {8-azaXMP}} 0.53 {ATP}} [C]

Substrates: ATP; L-Glutamine; H2O; Xanthosine 5'-phosphate [C]

Specific reaction: ATP + L-Glutamine + H2O + Xanthosine 5'-phosphate --> AMP + L-Glutamate + GMP + (2) H+ + Diphosphate [C]

General reaction: Amination [C]

Inhibitor: 1, N6-Ethenoadenosine; 1-Methyl adenosine 5'-phosphate 5'-phosphate; 2', 3'-Dihydroadenosine; 2'-Deoxyadenosine; 2, 6-Diaminopurine ribonucleoside; 2-Amino-6-methyl thiopurine ribonucleoside; 2-Fluoroadenosine; 3'-Deoxyadenosine; 5'-Azido-5'-deoxyadenosin

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA