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The map label for this gene is ilvB [H]

Identifier: 83591624

GI number: 83591624

Start: 348329

End: 350059

Strand: Direct

Name: ilvB [H]

Synonym: Rru_A0284

Alternate gene names: 83591624

Gene position: 348329-350059 (Clockwise)

Preceding gene: 83591623

Following gene: 83591625

Centisome position: 8.0

GC content: 67.71

Gene sequence:

>1731_bases
ATGGGAAGTATTGGATCCAATATTCAAAAACACAGTCGGGGAGTGTGCGCCGTGGTCCAGTCTTCCGTTGCATCGCGTAC
GGGCGGTGCCTTGCTTGCCGACTCGTTGATCGCCCTCGGCGCCGATACGGTCTATTGCGTGCCCGGCGAAAGCTTCCTGC
CCTTCCTCGATGCCGTTCACGACCGCACCGACGCCTTGCGGCTGGTGGTCTGCCGGCAGGAGGGCGGGGCGTCCTATATG
GCCGATGCCCATGCCAAGATGACCGGCAAGCCCGGGGTGTGCTTCGTCACCCGGGGGCCCGGCGCCTGCAATGCGGCGAT
CGGCGTTCATACCGCCTTTCAAGATTGCACGCCGATGCTGCTGTTCATCGGTCAGGTCGACCGGCCGATGATCGGCCGCG
AAGCCTTTCAGGAAGTCGATATGACGGCGTTTTTCGCGCCCTTGGCCAAATTCAGCGCCACCGCCGACAGCGCCGCCCGC
CTGCCCGAGCTGGTGGCGCGGGCCTGGGCGGCGGCGACCTCGGGGCGACCGGGACCGGCGGTGGTGGCCTTGCCCGAGGA
TGTTCTGGCCGAGATGGCCTTTGCCCGCGATCCGCTTCCCCGTCCCCCGGCGCGTCCGGCGCCGCGCGCGGCCGATATGG
CCGATCTGGCAAGCCGGTTGGCGGCGGCCGAGCGGCCGCTGGTCATCGTCGGCGGCGGCACCTGGACGCCGCGCGCGGCC
AAGCTGGTCACCACCTGGGCCGAGGCCAGCGGCCTGCCGGTGGTCGCCGCCTTCCGCCGCCAGGACGTGGTCGACAACCT
CAGCCCGGTCTATATCGGCGAACTGGGCTTTTCGGCCAGCCCGCGCCTTAACGCCCGCATCAAGGCCGCCGATCTGATCG
TTTGCGTCGGCGATCGACTGGGCGAGGTCACGACCCAGGGCTATAGCCTGATCGAGTCGCCGGTCCCCAGCCAAAGCCTG
ATCCATATTTTCCCCGATGCCGGTGAAATCGGCCGGGTCTATGCCACCGATCTGGGGATTGTTTCGGCGATGGAGCCCTT
CGCCGAGATGCTGAGCCCGGTCGATGGCCGTCATTGGGCCGGCTGGCTGGCCCAGGGGCGGGCAGACTACGCCGCCAATC
TGCGCGCCGACCCCTGCCCGGGTAGCGTGGACATGGCCCAGGTGATGGCCGAGATCGAGGCCCGCTTGCCCGAGGACGCG
GTGATCACCAATGGCGCGGGCAATTATTCGGGCTGGGTCCACCGCTTCCATCGCTATCGCCACTTCCCCAGCCAGTTGGC
GCCGGGCAACGGGTCGATGGGCTATGGCCTGCCCGCCGCCATCGCCGCCAAGGTCGTCGCCCCCCGGCGGGTGGTGGTGG
CCTTCGCCGGCGACGGTTGTTTCCTGATGAACGGTCAGGAACTGGCGACCGCCGTCGACCAGGGGCTTGATCCCATCATC
ATCGTCATCAACAACGGCATGTATGGCACCATCCGCATGCATCAGGAGAAGGCCTATCCCGGCCGGGTCAGCGGCACCAG
CCTGACCAACCCGGATTTCGTCGCCCTCGCCCAGGCCTATGGCGCCCTGGGCTTGCGCGTGCGCCGCACGGCCGAATTCG
GCCCGGCTTTTGATCAGGCCCTGGTCGCCGGCCGTCCGGTGTTGATCGAGATCCATCAAGACCCCGAGGCGATCAGCCAC
CGCGTCAGCTTGACCGCCCTGCGGGAAAAGGCCTTGGCCACCAGCGCCTGA

Upstream 100 bases:

>100_bases
GCCCTTTGCCCTGGCCGGGCGCGGAGCCCGCCGCCGCCGCAAAGTATCCTCCCACCCCACGGCCTGAGACCGCCAGCCCC
CTTGACCGCGGGGCCGGGGA

Downstream 100 bases:

>100_bases
TCGTCTGCTACCCCTTCGTCCGTTCCTCCCCATCCGCCCCCGGCCCGCGTCGGGGCTCGACCCGGAGACTTTGCCCATGG
CTTCCGCCTATGCCGTCGCC

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MGSIGSNIQKHSRGVCAVVQSSVASRTGGALLADSLIALGADTVYCVPGESFLPFLDAVHDRTDALRLVVCRQEGGASYM
ADAHAKMTGKPGVCFVTRGPGACNAAIGVHTAFQDCTPMLLFIGQVDRPMIGREAFQEVDMTAFFAPLAKFSATADSAAR
LPELVARAWAAATSGRPGPAVVALPEDVLAEMAFARDPLPRPPARPAPRAADMADLASRLAAAERPLVIVGGGTWTPRAA
KLVTTWAEASGLPVVAAFRRQDVVDNLSPVYIGELGFSASPRLNARIKAADLIVCVGDRLGEVTTQGYSLIESPVPSQSL
IHIFPDAGEIGRVYATDLGIVSAMEPFAEMLSPVDGRHWAGWLAQGRADYAANLRADPCPGSVDMAQVMAEIEARLPEDA
VITNGAGNYSGWVHRFHRYRHFPSQLAPGNGSMGYGLPAAIAAKVVAPRRVVVAFAGDGCFLMNGQELATAVDQGLDPII
IVINNGMYGTIRMHQEKAYPGRVSGTSLTNPDFVALAQAYGALGLRVRRTAEFGPAFDQALVAGRPVLIEIHQDPEAISH
RVSLTALREKALATSA

Sequences:

>Translated_576_residues
MGSIGSNIQKHSRGVCAVVQSSVASRTGGALLADSLIALGADTVYCVPGESFLPFLDAVHDRTDALRLVVCRQEGGASYM
ADAHAKMTGKPGVCFVTRGPGACNAAIGVHTAFQDCTPMLLFIGQVDRPMIGREAFQEVDMTAFFAPLAKFSATADSAAR
LPELVARAWAAATSGRPGPAVVALPEDVLAEMAFARDPLPRPPARPAPRAADMADLASRLAAAERPLVIVGGGTWTPRAA
KLVTTWAEASGLPVVAAFRRQDVVDNLSPVYIGELGFSASPRLNARIKAADLIVCVGDRLGEVTTQGYSLIESPVPSQSL
IHIFPDAGEIGRVYATDLGIVSAMEPFAEMLSPVDGRHWAGWLAQGRADYAANLRADPCPGSVDMAQVMAEIEARLPEDA
VITNGAGNYSGWVHRFHRYRHFPSQLAPGNGSMGYGLPAAIAAKVVAPRRVVVAFAGDGCFLMNGQELATAVDQGLDPII
IVINNGMYGTIRMHQEKAYPGRVSGTSLTNPDFVALAQAYGALGLRVRRTAEFGPAFDQALVAGRPVLIEIHQDPEAISH
RVSLTALREKALATSA
>Mature_575_residues
GSIGSNIQKHSRGVCAVVQSSVASRTGGALLADSLIALGADTVYCVPGESFLPFLDAVHDRTDALRLVVCRQEGGASYMA
DAHAKMTGKPGVCFVTRGPGACNAAIGVHTAFQDCTPMLLFIGQVDRPMIGREAFQEVDMTAFFAPLAKFSATADSAARL
PELVARAWAAATSGRPGPAVVALPEDVLAEMAFARDPLPRPPARPAPRAADMADLASRLAAAERPLVIVGGGTWTPRAAK
LVTTWAEASGLPVVAAFRRQDVVDNLSPVYIGELGFSASPRLNARIKAADLIVCVGDRLGEVTTQGYSLIESPVPSQSLI
HIFPDAGEIGRVYATDLGIVSAMEPFAEMLSPVDGRHWAGWLAQGRADYAANLRADPCPGSVDMAQVMAEIEARLPEDAV
ITNGAGNYSGWVHRFHRYRHFPSQLAPGNGSMGYGLPAAIAAKVVAPRRVVVAFAGDGCFLMNGQELATAVDQGLDPIII
VINNGMYGTIRMHQEKAYPGRVSGTSLTNPDFVALAQAYGALGLRVRRTAEFGPAFDQALVAGRPVLIEIHQDPEAISHR
VSLTALREKALATSA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=567, Percent_Identity=26.4550264550265, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI21361361, Length=563, Percent_Identity=26.6429840142096, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI87081685, Length=541, Percent_Identity=32.1626617375231, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1790104, Length=543, Percent_Identity=29.4659300184162, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=29.014598540146, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1787096, Length=541, Percent_Identity=25.3234750462107, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788716, Length=569, Percent_Identity=25.4833040421793, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=545, Percent_Identity=26.4220183486239, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=545, Percent_Identity=26.4220183486239, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17542570, Length=555, Percent_Identity=23.2432432432432, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=30.6338028169014, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6320816, Length=545, Percent_Identity=23.1192660550459, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922626, Length=578, Percent_Identity=25.6055363321799, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60736; Mature: 60605

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSIGSNIQKHSRGVCAVVQSSVASRTGGALLADSLIALGADTVYCVPGESFLPFLDAVH
CCCCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DRTDALRLVVCRQEGGASYMADAHAKMTGKPGVCFVTRGPGACNAAIGVHTAFQDCTPML
CCCCEEEEEEEECCCCCCEEECCCCEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LFIGQVDRPMIGREAFQEVDMTAFFAPLAKFSATADSAARLPELVARAWAAATSGRPGPA
HHHCCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCE
VVALPEDVLAEMAFARDPLPRPPARPAPRAADMADLASRLAAAERPLVIVGGGTWTPRAA
EEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHH
KLVTTWAEASGLPVVAAFRRQDVVDNLSPVYIGELGFSASPRLNARIKAADLIVCVGDRL
HHEEEHHHCCCCCEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEEEECCCHH
GEVTTQGYSLIESPVPSQSLIHIFPDAGEIGRVYATDLGIVSAMEPFAEMLSPVDGRHWA
CHHHHHCHHHHCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHH
GWLAQGRADYAANLRADPCPGSVDMAQVMAEIEARLPEDAVITNGAGNYSGWVHRFHRYR
HHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHH
HFPSQLAPGNGSMGYGLPAAIAAKVVAPRRVVVAFAGDGCFLMNGQELATAVDQGLDPII
CCHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEEECCHHHHHHHHCCCCEEE
IVINNGMYGTIRMHQEKAYPGRVSGTSLTNPDFVALAQAYGALGLRVRRTAEFGPAFDQA
EEEECCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHH
LVAGRPVLIEIHQDPEAISHRVSLTALREKALATSA
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GSIGSNIQKHSRGVCAVVQSSVASRTGGALLADSLIALGADTVYCVPGESFLPFLDAVH
CCCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHH
DRTDALRLVVCRQEGGASYMADAHAKMTGKPGVCFVTRGPGACNAAIGVHTAFQDCTPML
CCCCEEEEEEEECCCCCCEEECCCCEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LFIGQVDRPMIGREAFQEVDMTAFFAPLAKFSATADSAARLPELVARAWAAATSGRPGPA
HHHCCCCCCHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCE
VVALPEDVLAEMAFARDPLPRPPARPAPRAADMADLASRLAAAERPLVIVGGGTWTPRAA
EEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHH
KLVTTWAEASGLPVVAAFRRQDVVDNLSPVYIGELGFSASPRLNARIKAADLIVCVGDRL
HHEEEHHHCCCCCEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEEEECCCHH
GEVTTQGYSLIESPVPSQSLIHIFPDAGEIGRVYATDLGIVSAMEPFAEMLSPVDGRHWA
CHHHHHCHHHHCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCHHH
GWLAQGRADYAANLRADPCPGSVDMAQVMAEIEARLPEDAVITNGAGNYSGWVHRFHRYR
HHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHH
HFPSQLAPGNGSMGYGLPAAIAAKVVAPRRVVVAFAGDGCFLMNGQELATAVDQGLDPII
CCHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCEEEECCHHHHHHHHCCCCEEE
IVINNGMYGTIRMHQEKAYPGRVSGTSLTNPDFVALAQAYGALGLRVRRTAEFGPAFDQA
EEEECCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHH
LVAGRPVLIEIHQDPEAISHRVSLTALREKALATSA
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]