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The map label for this gene is slt [H]

Identifier: 83591607

GI number: 83591607

Start: 331970

End: 333694

Strand: Direct

Name: slt [H]

Synonym: Rru_A0267

Alternate gene names: 83591607

Gene position: 331970-333694 (Clockwise)

Preceding gene: 83591606

Following gene: 83591608

Centisome position: 7.63

GC content: 69.51

Gene sequence:

>1725_bases
ATGGGGATGTTTCCGGTCGGCCGCTGGGTTCGGACCGTGGCTTTGGGCTGCTGCCTGATGGCGGGCGCCGGATCGGCCGC
CGCCAAGCCGCCGGGGGCGGTGCTCGGCCAAGCCGATGTGGCGCGCTATCGCACCATCTTCGCCCTTCAGGACAAGGGGC
TGTGGTCGGATGCCGACCGCGAGATCCTTGGGCTTGAGGATCCGCTGCTGATGGGGCATGTCCGCTATCAGCGCCTGATG
CATCCCACCGCCTATCGGGCCAATGCCGACGAGTTGCGGACATGGCTGGCGACCTATGTCGACCATCCCTGCGGTCAACG
GCTGTTGCGGCTGGCCCAGCGCCGGGGCGTGGCGCCGCCGATCGATCCCGATGCCCCGCTGTTTGGCGATCTGGGCAGCG
GAGAGGTGGGCGACGGGCCGGGCTGGGGTTTGGAGGGCGTCTATCCGCCGGGCCAACGCCCCACCGCCCAGGAGCGCTGG
GAGATTTTCCTGCAAGCCCTGGGGGCGGGGCAGACCCTGCGGATGAAGGAGCTGATCACCGGGGCGCGCAATGGCGTGCT
CGCCGATATCGACCAGGACCGCATGAAGGCGGCGCTGGCCTTCGCCTATTTCGTCGAGGGCCGCGATGAGTGGGCGATCG
CGTGGGCGCGCGAGGCGATCGCCCGTTCGGGCAAGCGTTTGCCGATCGCCCATTGGGCCGGCGGTCTGGCGCTGTGGCGG
AGCGGCAAGCCGGCCGAGGCCCTGGGCCATTTCGAAGAGGTGATCCGCGCCCCCGGGGCGTCCCCCTGGCTACGCTCGGC
CGGAGCCTTCTGGGCGGCGCGCAGCGCCCTGCGGGCCCGCCATCCCGAGCGGGTCAGCCTTTTGCTTGAACGGGCGGCGG
CCGAGCCGCGCACGTTCTATGGGCTGGTGGCGCTGCGCGCCCTTGGCCGGCCGATGCCGTTTTCCTGGAACGCCCCAAAG
CTTTCGGAGCGCGCCTGGGAGCGGCTGGAGCGGTTGCCCGGGGCGCGGCGGGCGATGGCCCTGGTGCAGATCGGCCGATC
GCGCGAGGCGGCCGAGGAACTGACCCCGCTTTACGCCGCCGCCGGTCCCGAACTGCGCGATGCCCTGTTGGGGGCCACCC
TGGCCCTGGGCCTGCCCACTCTGGCGCTCGACATCGGCCGCACCGATCCCCGGGCCGGCGATGTCGCGCGCTTTCCCCTG
CCGCCCTGGTCGCCCGATGTCGGCTGGTCGCTTGACCGGGCGCTGATCTATGCCTTCGCCCGCCAGGAAAGCGCCTTCGA
TCCCCAGGCGGTCAGTCCGGCCGGGGCCTTGGGGCTGATGCAGTTGATGCCGGCCACCGCCTCGCATGTCGCCGGCGACC
GGGCTTTGGCCGATGCCGAAGGCGGCGAGGTTTTGTTCCAGCCGACGGTCAATCTCAGTTTGGGGCAGGCCTATCTGGCC
GAGCTTCTGGCCAGTTCGCAAATCCGGGGCAATCTTTTCTTTCTAGCCACCGCCTATAACAGCGGTCCGGGTCGCTTGGC
CGGCTGGCTGCGCGAAGGGCGCTATGACGAGGATCCGCTGCTGTTCATCGAAAGCATCGGCAGCCGAGAAACCAGACTGT
TCATCGAACGGGTTCTGACCAATTATTGGGCCTACCGCTCGCGTCTTGATCAATCGCTTTCCTCGCTTGACGCGGTCGCC
GCCGGGGACTGGCCGCGCTATCAGGCCGCCAAGCCGGCGCGCTGA

Upstream 100 bases:

>100_bases
GCCTGTCATAAGGACAAGGCCCGCGTCCCGGGGCTGACTTGGGGCCGGGGGACGGCTATAAACCGCTGCGGGGATTTCAG
GGGCGAAGGGGCTTTACCTC

Downstream 100 bases:

>100_bases
GTTCCCCTTCTTCTCCCCCGTCTGTAAGGACCTTCCCCCATGGCCGGTATCGACGAGTCCAAGCCTTTCCTGCCCGTGCG
CATCGCCATTTTGACGGTTT

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MGMFPVGRWVRTVALGCCLMAGAGSAAAKPPGAVLGQADVARYRTIFALQDKGLWSDADREILGLEDPLLMGHVRYQRLM
HPTAYRANADELRTWLATYVDHPCGQRLLRLAQRRGVAPPIDPDAPLFGDLGSGEVGDGPGWGLEGVYPPGQRPTAQERW
EIFLQALGAGQTLRMKELITGARNGVLADIDQDRMKAALAFAYFVEGRDEWAIAWAREAIARSGKRLPIAHWAGGLALWR
SGKPAEALGHFEEVIRAPGASPWLRSAGAFWAARSALRARHPERVSLLLERAAAEPRTFYGLVALRALGRPMPFSWNAPK
LSERAWERLERLPGARRAMALVQIGRSREAAEELTPLYAAAGPELRDALLGATLALGLPTLALDIGRTDPRAGDVARFPL
PPWSPDVGWSLDRALIYAFARQESAFDPQAVSPAGALGLMQLMPATASHVAGDRALADAEGGEVLFQPTVNLSLGQAYLA
ELLASSQIRGNLFFLATAYNSGPGRLAGWLREGRYDEDPLLFIESIGSRETRLFIERVLTNYWAYRSRLDQSLSSLDAVA
AGDWPRYQAAKPAR

Sequences:

>Translated_574_residues
MGMFPVGRWVRTVALGCCLMAGAGSAAAKPPGAVLGQADVARYRTIFALQDKGLWSDADREILGLEDPLLMGHVRYQRLM
HPTAYRANADELRTWLATYVDHPCGQRLLRLAQRRGVAPPIDPDAPLFGDLGSGEVGDGPGWGLEGVYPPGQRPTAQERW
EIFLQALGAGQTLRMKELITGARNGVLADIDQDRMKAALAFAYFVEGRDEWAIAWAREAIARSGKRLPIAHWAGGLALWR
SGKPAEALGHFEEVIRAPGASPWLRSAGAFWAARSALRARHPERVSLLLERAAAEPRTFYGLVALRALGRPMPFSWNAPK
LSERAWERLERLPGARRAMALVQIGRSREAAEELTPLYAAAGPELRDALLGATLALGLPTLALDIGRTDPRAGDVARFPL
PPWSPDVGWSLDRALIYAFARQESAFDPQAVSPAGALGLMQLMPATASHVAGDRALADAEGGEVLFQPTVNLSLGQAYLA
ELLASSQIRGNLFFLATAYNSGPGRLAGWLREGRYDEDPLLFIESIGSRETRLFIERVLTNYWAYRSRLDQSLSSLDAVA
AGDWPRYQAAKPAR
>Mature_573_residues
GMFPVGRWVRTVALGCCLMAGAGSAAAKPPGAVLGQADVARYRTIFALQDKGLWSDADREILGLEDPLLMGHVRYQRLMH
PTAYRANADELRTWLATYVDHPCGQRLLRLAQRRGVAPPIDPDAPLFGDLGSGEVGDGPGWGLEGVYPPGQRPTAQERWE
IFLQALGAGQTLRMKELITGARNGVLADIDQDRMKAALAFAYFVEGRDEWAIAWAREAIARSGKRLPIAHWAGGLALWRS
GKPAEALGHFEEVIRAPGASPWLRSAGAFWAARSALRARHPERVSLLLERAAAEPRTFYGLVALRALGRPMPFSWNAPKL
SERAWERLERLPGARRAMALVQIGRSREAAEELTPLYAAAGPELRDALLGATLALGLPTLALDIGRTDPRAGDVARFPLP
PWSPDVGWSLDRALIYAFARQESAFDPQAVSPAGALGLMQLMPATASHVAGDRALADAEGGEVLFQPTVNLSLGQAYLAE
LLASSQIRGNLFFLATAYNSGPGRLAGWLREGRYDEDPLLFIESIGSRETRLFIERVLTNYWAYRSRLDQSLSSLDAVAA
GDWPRYQAAKPAR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=132, Percent_Identity=37.8787878787879, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 62562; Mature: 62431

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMFPVGRWVRTVALGCCLMAGAGSAAAKPPGAVLGQADVARYRTIFALQDKGLWSDADR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCCCCCC
EILGLEDPLLMGHVRYQRLMHPTAYRANADELRTWLATYVDHPCGQRLLRLAQRRGVAPP
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
IDPDAPLFGDLGSGEVGDGPGWGLEGVYPPGQRPTAQERWEIFLQALGAGQTLRMKELIT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
GARNGVLADIDQDRMKAALAFAYFVEGRDEWAIAWAREAIARSGKRLPIAHWAGGLALWR
HHCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHEEC
SGKPAEALGHFEEVIRAPGASPWLRSAGAFWAARSALRARHPERVSLLLERAAAEPRTFY
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
GLVALRALGRPMPFSWNAPKLSERAWERLERLPGARRAMALVQIGRSREAAEELTPLYAA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AGPELRDALLGATLALGLPTLALDIGRTDPRAGDVARFPLPPWSPDVGWSLDRALIYAFA
CCHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHH
RQESAFDPQAVSPAGALGLMQLMPATASHVAGDRALADAEGGEVLFQPTVNLSLGQAYLA
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCHHHHHHHH
ELLASSQIRGNLFFLATAYNSGPGRLAGWLREGRYDEDPLLFIESIGSRETRLFIERVLT
HHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEHHHHCCCHHHHHHHHHHHH
NYWAYRSRLDQSLSSLDAVAAGDWPRYQAAKPAR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GMFPVGRWVRTVALGCCLMAGAGSAAAKPPGAVLGQADVARYRTIFALQDKGLWSDADR
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEEEECCCCCCCCCC
EILGLEDPLLMGHVRYQRLMHPTAYRANADELRTWLATYVDHPCGQRLLRLAQRRGVAPP
CCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
IDPDAPLFGDLGSGEVGDGPGWGLEGVYPPGQRPTAQERWEIFLQALGAGQTLRMKELIT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
GARNGVLADIDQDRMKAALAFAYFVEGRDEWAIAWAREAIARSGKRLPIAHWAGGLALWR
HHCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCHHEEC
SGKPAEALGHFEEVIRAPGASPWLRSAGAFWAARSALRARHPERVSLLLERAAAEPRTFY
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
GLVALRALGRPMPFSWNAPKLSERAWERLERLPGARRAMALVQIGRSREAAEELTPLYAA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
AGPELRDALLGATLALGLPTLALDIGRTDPRAGDVARFPLPPWSPDVGWSLDRALIYAFA
CCHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHH
RQESAFDPQAVSPAGALGLMQLMPATASHVAGDRALADAEGGEVLFQPTVNLSLGQAYLA
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEEEECCCCCHHHHHHHH
ELLASSQIRGNLFFLATAYNSGPGRLAGWLREGRYDEDPLLFIESIGSRETRLFIERVLT
HHHHHCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCEEHHHHCCCHHHHHHHHHHHH
NYWAYRSRLDQSLSSLDAVAAGDWPRYQAAKPAR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]