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The map label for this gene is mdtK [H]

Identifier: 83591532

GI number: 83591532

Start: 233826

End: 235253

Strand: Direct

Name: mdtK [H]

Synonym: Rru_A0192

Alternate gene names: 83591532

Gene position: 233826-235253 (Clockwise)

Preceding gene: 83591527

Following gene: 83591534

Centisome position: 5.37

GC content: 64.92

Gene sequence:

>1428_bases
ATGACCCAAACCCCGACCGCAGGACCCGATGCAGGCCGCCCGGCGTCCGTCCTGCCACCCCTGCTGTTGCTTTCGGCTCC
CATCATCATCTCCCAATGCGCGCAGATGGCCAGCGGTGTGGTGGATGTGATTATGGCCGGCAACCTCGATGTGCTGACGT
TGGGCACGGTCGCCACCGGAGCCGCCCTCTGGGTGCCGATGATGATCTTTCTTCTGGGCCTGCTCTACGGCGTAACCCCG
ACGATCGGCAAGATGCTGGGGGAGAATGCGTCGGAGGCCGTGTCCGGCGTGGCGGCGCGCGGCTTGCTGGTCGGCCTCCT
CGGGGGGGCGTTGCTGGGAGCGCTGCTGTTTTGGATGTCCACGCCGTTGTTCCGCCTTTTCGGGGTGGCGCCGGAGCTGA
TTCCCGAGGCCTCCGCCTACCTGAACTGGATGGCCTTGGCCATGCCCGGCATGGGGCTTTTCCTGGCCTTTAGGTTCGTT
ATCGAAGCCCATCACGTCGGTTGGATCGTGACAGTGGTGGCCGTTTTGGGCGTGGCCGCCAAAATCTGGCTGAACGACGC
CCTGGTTCATGGACATGCCGGCCTGCCGGCCCTTGGAACCGGTGGGTTCGGCGCGGCCTCGGTGGCGCTGTATTGGGGGA
TGGCGGTGATGCTGGCGGGCGCCACTTTTCTGCACGGGGCGGTCAAGCCCTTGTGGACTCACCGTCTGTCGGGCGCCGAC
CTGTCGCTCGCCGCGATCGGCCGGTTTTTACGGTTCGGCTTGCCCATCGCCCTGAACTTCCTGTCCGACTACCTGATCAT
GGCGGTGGTGGCGGTGTTCATCGCCACGCTGGGCGCGGTTGCCATCGGCGCTCATCAGATCACCTTCAATATATTGATGG
TTTTGTTGATGATGCCCACCGGACTTAGCATGGCGGCGACGATTCTGATCTCGCGATCCCCGGGCGGCGGGCACACGGCC
ACCCTCGGCCCGGTGATGAGCTGGACGCTGGGGATTGGCGTTGCGTTTTCCATCGTCCTTGCCGTCCTGCTGGCGGCGTT
CGATGCGCCGGTGATCCGCCTTTATACCGACGATGCGGAGGTTATCGCCCTAGCCCTCGACCTGCTGGGGGTCGTCGTGT
TTATCCTCACCATCGACGTGGTGGCCATCGCCTTGGGATTTTTCCTGCGCGGGCTGGGTGATCCGGGCAGCCCCTTCCTG
ATTTTGCTGGCGGCGCATTGGCTGATCAGCCTGCCTGTGGGGTACGTGCTGTGCTTCACGGACTGGGTCGTGGCGCCGCA
AGGTCCTCTCGGCTGGTGGTACGGGCTGTCCACCGGTTTGCTGGTCGCATGCTGCCTCTTGGGGCTTCGTCTCCGGCAAA
GGCTAAGTCGTGCCGCCCAGCCGGTCTGTTCCCCGGCCGAGGCGACGGTGGCCGGGGTCGCGGAGTAG

Upstream 100 bases:

>100_bases
CATGCCTGCGTGAACGACATGCGGTAGGGGCTGTCCTTCGGTCTGGCTCCCGATACGAGAGTCCCATCATGAAAGACGAC
GTGAGGATGGAGGACGGTTC

Downstream 100 bases:

>100_bases
GGGGGTGGCGTGATTCCTGTTCCCGAACCCGCGCCCGTGGTCCGGCTCTGGTCCCTGGAGCCGTTCCCGATCCGCTTCCG
TCCGAATTTCGGCGATGTCT

Product: multi anti extrusion protein MatE

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTQTPTAGPDAGRPASVLPPLLLLSAPIIISQCAQMASGVVDVIMAGNLDVLTLGTVATGAALWVPMMIFLLGLLYGVTP
TIGKMLGENASEAVSGVAARGLLVGLLGGALLGALLFWMSTPLFRLFGVAPELIPEASAYLNWMALAMPGMGLFLAFRFV
IEAHHVGWIVTVVAVLGVAAKIWLNDALVHGHAGLPALGTGGFGAASVALYWGMAVMLAGATFLHGAVKPLWTHRLSGAD
LSLAAIGRFLRFGLPIALNFLSDYLIMAVVAVFIATLGAVAIGAHQITFNILMVLLMMPTGLSMAATILISRSPGGGHTA
TLGPVMSWTLGIGVAFSIVLAVLLAAFDAPVIRLYTDDAEVIALALDLLGVVVFILTIDVVAIALGFFLRGLGDPGSPFL
ILLAAHWLISLPVGYVLCFTDWVVAPQGPLGWWYGLSTGLLVACCLLGLRLRQRLSRAAQPVCSPAEATVAGVAE

Sequences:

>Translated_475_residues
MTQTPTAGPDAGRPASVLPPLLLLSAPIIISQCAQMASGVVDVIMAGNLDVLTLGTVATGAALWVPMMIFLLGLLYGVTP
TIGKMLGENASEAVSGVAARGLLVGLLGGALLGALLFWMSTPLFRLFGVAPELIPEASAYLNWMALAMPGMGLFLAFRFV
IEAHHVGWIVTVVAVLGVAAKIWLNDALVHGHAGLPALGTGGFGAASVALYWGMAVMLAGATFLHGAVKPLWTHRLSGAD
LSLAAIGRFLRFGLPIALNFLSDYLIMAVVAVFIATLGAVAIGAHQITFNILMVLLMMPTGLSMAATILISRSPGGGHTA
TLGPVMSWTLGIGVAFSIVLAVLLAAFDAPVIRLYTDDAEVIALALDLLGVVVFILTIDVVAIALGFFLRGLGDPGSPFL
ILLAAHWLISLPVGYVLCFTDWVVAPQGPLGWWYGLSTGLLVACCLLGLRLRQRLSRAAQPVCSPAEATVAGVAE
>Mature_474_residues
TQTPTAGPDAGRPASVLPPLLLLSAPIIISQCAQMASGVVDVIMAGNLDVLTLGTVATGAALWVPMMIFLLGLLYGVTPT
IGKMLGENASEAVSGVAARGLLVGLLGGALLGALLFWMSTPLFRLFGVAPELIPEASAYLNWMALAMPGMGLFLAFRFVI
EAHHVGWIVTVVAVLGVAAKIWLNDALVHGHAGLPALGTGGFGAASVALYWGMAVMLAGATFLHGAVKPLWTHRLSGADL
SLAAIGRFLRFGLPIALNFLSDYLIMAVVAVFIATLGAVAIGAHQITFNILMVLLMMPTGLSMAATILISRSPGGGHTAT
LGPVMSWTLGIGVAFSIVLAVLLAAFDAPVIRLYTDDAEVIALALDLLGVVVFILTIDVVAIALGFFLRGLGDPGSPFLI
LLAAHWLISLPVGYVLCFTDWVVAPQGPLGWWYGLSTGLLVACCLLGLRLRQRLSRAAQPVCSPAEATVAGVAE

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=226, Percent_Identity=24.7787610619469, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI48994890, Length=443, Percent_Identity=32.0541760722348, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49227; Mature: 49095

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TQTPTAGPDAGRPASVLPPLLLLSAPIIISQCAQMASGVVDVIMAGNLDVLTLGTVATG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
AALWVPMMIFLLGLLYGVTPTIGKMLGENASEAVSGVAARGLLVGLLGGALLGALLFWMS
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPLFRLFGVAPELIPEASAYLNWMALAMPGMGLFLAFRFVIEAHHVGWIVTVVAVLGVAA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIWLNDALVHGHAGLPALGTGGFGAASVALYWGMAVMLAGATFLHGAVKPLWTHRLSGAD
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSLAAIGRFLRFGLPIALNFLSDYLIMAVVAVFIATLGAVAIGAHQITFNILMVLLMMPT
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GLSMAATILISRSPGGGHTATLGPVMSWTLGIGVAFSIVLAVLLAAFDAPVIRLYTDDAE
CHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHH
VIALALDLLGVVVFILTIDVVAIALGFFLRGLGDPGSPFLILLAAHWLISLPVGYVLCFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DWVVAPQGPLGWWYGLSTGLLVACCLLGLRLRQRLSRAAQPVCSPAEATVAGVAE
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA