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The map label for this gene is ptsI [H]

Identifier: 83313492

GI number: 83313492

Start: 4795677

End: 4797467

Strand: Reverse

Name: ptsI [H]

Synonym: amb4393

Alternate gene names: 83313492

Gene position: 4797467-4795677 (Counterclockwise)

Preceding gene: 83313493

Following gene: 83313491

Centisome position: 96.58

GC content: 69.07

Gene sequence:

>1791_bases
ATGACGGCGAACGGCGGCAGCAAGGCGGTCGGACGAAGCAAGGCGACGGCCCGCGCCGAACTGGTCCGCGACGGGTTGGG
CGTGTCGCGGGGCATCGCCATCGGCACCCTCTATCTGCACGACGCCGGGACCATCACCGTCCCCGAGCTGCGCATTCCCG
CCGCCCGTCTGGATGCCGAATGCGCCCGCTTCAACGAGGCCTCGGCCAATGCGGGCCGTCAGGTCGAGCAGTTGCAGGAC
AAGGCGGGGCGCCTTGGCGGGGCCGCCGGCGAGGAACTGGGCTACCTGCTGGATGCCTATCGCCAGATGCTGCACGGCTC
GCGCCTGCTGCGGGGCGTCGAGGCCCGCATCCGCAACGACCGCATCAATGCCGAGGCCGCCGTTCAGCAGGAGATCAACG
AGATCGTCCGTGGCTTCGAGGCCATGGAGGACCCGTATCTCGCCGCCCGCGTGGCCGACATCAAGGATATCGGCCGCCGT
TTGCTGCGCGCCCTGACCAATACCGCCTATCGTCCCTTTACCGCCTTGCCCAGGAACGCGGTGATCGTCGCCGAGGAGAT
GACCCCCGCCGACACCGCCCTGCTTGATCCCAATCAGGTGGCCGGACTGGCCACCGTGATGGGCGGCAGCGAGGGCCATA
CCGCCATCATGGCGCGCTCCCTGGGGCTGCCCTCGGTGCTGGGCATCGCCGATCTGCTGACCGGCGTGAAGGGCGGCGAA
CAGGTCATCATCGACGGCACCAACGGCCTGGTGGTGATCAATCCCGGACCGGAGACCCTGGCCTTCTACCGGCGCGAACG
CGCCGCCTTCCTCAAGGCGCGCCGCGTGCTGTCCCGCCTCAAGGACGTGCCGGCCGTGACCCGCGACGGCGGACGCATCA
CGCTGCAGGCCAATATGGAGCTGCCCAACGAAGTGGGTGCCGTGCTGGACTCCGGGGCCGAGGGCATCGGCCTGCTGCGC
AGCGAGTTCCTGTACATGAACCGCGACGACGTCCCCAGCGAGGACGAGCAATACGCCATCCTCAAGGACGTGGTCGAGCG
CATGGGCGGCCGCACGGTCACGGTGCGGACCTTCGATGCCGGTGGCGACAAGCTGGCCCCCGCCCTGGGCTGTACCATCG
GCCCCAATCCCTCGCTGGGCCTGCGCGCCATCCGCCTGGGGCTGGCCCGCCAGGACCTGCTCGAGGCCCAGCTGGCCGCC
ATCCTGCGGGCGTCCGCCCATGGCCCGGTGCGCATCCTGATCCCGATGATCGCCACGGTGGGCGAGCTGCGCGCCACCCG
CGAGGTGATGAACCGGGTGATCCGCCGCCTGCGCAAGGCGGGCGTGAGCCTGCCAGATCCGCTGCCCCGGCTGGGCGCCA
TGATCGAGATTCCCGGCGCGGCGCTGTCGGCGGATTCCATCGCCTGGCATGCCGATTTCTTCGCCATCGGCACCAATGAC
CTGGTGCAGTACACCCTGGCCATCGACCGGTCCGACGAGGCGGTGGCTCATCTTTACAACCCGCTGCATCCGGCGGTGTT
GCGCCTGATCCAGTTCACCACCCAGGCGGCTCAGCGGGCCAATATCCCGGTCAGCGTCTGCGGCGAGATGGCGGGCGATC
CCCGCTACGCTCCCTTGCTGCTGGGGCTCGGCCTGCGCGACCTGTCCATGAGCACGTCGAACCTGCCGGTGGTGAAGAAC
CGCATCCGCTCGCTCGATCTGGCCGCCGCCGAGACCCTGGCCCGCACGGTCATGGAACAGACCGATTCTGGCAAGATCGG
CCAGTTGCTCGACGGCTTCCAGGGACATTGA

Upstream 100 bases:

>100_bases
TCCATCGAGCTGAAGGTGACCGGCGCCCAGGCCACCGAAGCCATGGACGCCCTGTTCGATCTCGTCGACCGCAAGTTCGA
CGAAGACTGAAAGCCCGGTC

Downstream 100 bases:

>100_bases
GACCAAAGAGGGGGGGAACTATGCGTATCCTGATCGTCGCCGCAATGGCGCTGCTGCTGGGCGGCTCCGCCCTGGCCGAG
GAGGCCTATCCCTCGGTTCA

Product: phosphoenolpyruvate-protein kinase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MTANGGSKAVGRSKATARAELVRDGLGVSRGIAIGTLYLHDAGTITVPELRIPAARLDAECARFNEASANAGRQVEQLQD
KAGRLGGAAGEELGYLLDAYRQMLHGSRLLRGVEARIRNDRINAEAAVQQEINEIVRGFEAMEDPYLAARVADIKDIGRR
LLRALTNTAYRPFTALPRNAVIVAEEMTPADTALLDPNQVAGLATVMGGSEGHTAIMARSLGLPSVLGIADLLTGVKGGE
QVIIDGTNGLVVINPGPETLAFYRRERAAFLKARRVLSRLKDVPAVTRDGGRITLQANMELPNEVGAVLDSGAEGIGLLR
SEFLYMNRDDVPSEDEQYAILKDVVERMGGRTVTVRTFDAGGDKLAPALGCTIGPNPSLGLRAIRLGLARQDLLEAQLAA
ILRASAHGPVRILIPMIATVGELRATREVMNRVIRRLRKAGVSLPDPLPRLGAMIEIPGAALSADSIAWHADFFAIGTND
LVQYTLAIDRSDEAVAHLYNPLHPAVLRLIQFTTQAAQRANIPVSVCGEMAGDPRYAPLLLGLGLRDLSMSTSNLPVVKN
RIRSLDLAAAETLARTVMEQTDSGKIGQLLDGFQGH

Sequences:

>Translated_596_residues
MTANGGSKAVGRSKATARAELVRDGLGVSRGIAIGTLYLHDAGTITVPELRIPAARLDAECARFNEASANAGRQVEQLQD
KAGRLGGAAGEELGYLLDAYRQMLHGSRLLRGVEARIRNDRINAEAAVQQEINEIVRGFEAMEDPYLAARVADIKDIGRR
LLRALTNTAYRPFTALPRNAVIVAEEMTPADTALLDPNQVAGLATVMGGSEGHTAIMARSLGLPSVLGIADLLTGVKGGE
QVIIDGTNGLVVINPGPETLAFYRRERAAFLKARRVLSRLKDVPAVTRDGGRITLQANMELPNEVGAVLDSGAEGIGLLR
SEFLYMNRDDVPSEDEQYAILKDVVERMGGRTVTVRTFDAGGDKLAPALGCTIGPNPSLGLRAIRLGLARQDLLEAQLAA
ILRASAHGPVRILIPMIATVGELRATREVMNRVIRRLRKAGVSLPDPLPRLGAMIEIPGAALSADSIAWHADFFAIGTND
LVQYTLAIDRSDEAVAHLYNPLHPAVLRLIQFTTQAAQRANIPVSVCGEMAGDPRYAPLLLGLGLRDLSMSTSNLPVVKN
RIRSLDLAAAETLARTVMEQTDSGKIGQLLDGFQGH
>Mature_595_residues
TANGGSKAVGRSKATARAELVRDGLGVSRGIAIGTLYLHDAGTITVPELRIPAARLDAECARFNEASANAGRQVEQLQDK
AGRLGGAAGEELGYLLDAYRQMLHGSRLLRGVEARIRNDRINAEAAVQQEINEIVRGFEAMEDPYLAARVADIKDIGRRL
LRALTNTAYRPFTALPRNAVIVAEEMTPADTALLDPNQVAGLATVMGGSEGHTAIMARSLGLPSVLGIADLLTGVKGGEQ
VIIDGTNGLVVINPGPETLAFYRRERAAFLKARRVLSRLKDVPAVTRDGGRITLQANMELPNEVGAVLDSGAEGIGLLRS
EFLYMNRDDVPSEDEQYAILKDVVERMGGRTVTVRTFDAGGDKLAPALGCTIGPNPSLGLRAIRLGLARQDLLEAQLAAI
LRASAHGPVRILIPMIATVGELRATREVMNRVIRRLRKAGVSLPDPLPRLGAMIEIPGAALSADSIAWHADFFAIGTNDL
VQYTLAIDRSDEAVAHLYNPLHPAVLRLIQFTTQAAQRANIPVSVCGEMAGDPRYAPLLLGLGLRDLSMSTSNLPVVKNR
IRSLDLAAAETLARTVMEQTDSGKIGQLLDGFQGH

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=38.7040280210158, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1789193, Length=515, Percent_Identity=34.1747572815534, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=35.6573705179283, Blast_Score=272, Evalue=6e-74,
Organism=Escherichia coli, GI1788726, Length=569, Percent_Identity=31.4586994727592, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1787994, Length=407, Percent_Identity=24.0786240786241, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI226510935, Length=155, Percent_Identity=25.1612903225806, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63749; Mature: 63618

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANGGSKAVGRSKATARAELVRDGLGVSRGIAIGTLYLHDAGTITVPELRIPAARLDAE
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEECCCCCCCHHHHHHH
CARFNEASANAGRQVEQLQDKAGRLGGAAGEELGYLLDAYRQMLHGSRLLRGVEARIRND
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RINAEAAVQQEINEIVRGFEAMEDPYLAARVADIKDIGRRLLRALTNTAYRPFTALPRNA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
VIVAEEMTPADTALLDPNQVAGLATVMGGSEGHTAIMARSLGLPSVLGIADLLTGVKGGE
EEEEECCCCCCCEECCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCC
QVIIDGTNGLVVINPGPETLAFYRRERAAFLKARRVLSRLKDVPAVTRDGGRITLQANME
EEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCC
LPNEVGAVLDSGAEGIGLLRSEFLYMNRDDVPSEDEQYAILKDVVERMGGRTVTVRTFDA
CCHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECC
GGDKLAPALGCTIGPNPSLGLRAIRLGLARQDLLEAQLAAILRASAHGPVRILIPMIATV
CCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
GELRATREVMNRVIRRLRKAGVSLPDPLPRLGAMIEIPGAALSADSIAWHADFFAIGTND
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEECCCCCCCCCCEEEECEEEEECCHH
LVQYTLAIDRSDEAVAHLYNPLHPAVLRLIQFTTQAAQRANIPVSVCGEMAGDPRYAPLL
HEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHH
LGLGLRDLSMSTSNLPVVKNRIRSLDLAAAETLARTVMEQTDSGKIGQLLDGFQGH
HHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
>Mature Secondary Structure 
TANGGSKAVGRSKATARAELVRDGLGVSRGIAIGTLYLHDAGTITVPELRIPAARLDAE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEECCCCCCCHHHHHHH
CARFNEASANAGRQVEQLQDKAGRLGGAAGEELGYLLDAYRQMLHGSRLLRGVEARIRND
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RINAEAAVQQEINEIVRGFEAMEDPYLAARVADIKDIGRRLLRALTNTAYRPFTALPRNA
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
VIVAEEMTPADTALLDPNQVAGLATVMGGSEGHTAIMARSLGLPSVLGIADLLTGVKGGE
EEEEECCCCCCCEECCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCC
QVIIDGTNGLVVINPGPETLAFYRRERAAFLKARRVLSRLKDVPAVTRDGGRITLQANME
EEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCC
LPNEVGAVLDSGAEGIGLLRSEFLYMNRDDVPSEDEQYAILKDVVERMGGRTVTVRTFDA
CCHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECC
GGDKLAPALGCTIGPNPSLGLRAIRLGLARQDLLEAQLAAILRASAHGPVRILIPMIATV
CCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHH
GELRATREVMNRVIRRLRKAGVSLPDPLPRLGAMIEIPGAALSADSIAWHADFFAIGTND
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEECCCCCCCCCCEEEECEEEEECCHH
LVQYTLAIDRSDEAVAHLYNPLHPAVLRLIQFTTQAAQRANIPVSVCGEMAGDPRYAPLL
HEEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHH
LGLGLRDLSMSTSNLPVVKNRIRSLDLAAAETLARTVMEQTDSGKIGQLLDGFQGH
HHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7686067; 9384377; 10568751; 2497294; 8449881; 1495386 [H]