Click here to switch to the map view.

The map label for this gene is folD

Identifier: 83313473

GI number: 83313473

Start: 4778640

End: 4779527

Strand: Reverse

Name: folD

Synonym: amb4374

Alternate gene names: 83313473

Gene position: 4779527-4778640 (Counterclockwise)

Preceding gene: 83313474

Following gene: 83313472

Centisome position: 96.22

GC content: 67.0

Gene sequence:

>888_bases
ATGACTGACGCGAAGCTGATCGACGGCAACCTGTTTTCCGCCAAACTGCGCGAGACCATCACGGCCCGCGTGGCCGAGAT
GAAGGCGGCCCATCACATCACCCCCGGCTTGGCGGTGGTGCTGGTGGGCGAGGACCCGGCCAGCCAGGTCTATGTGCGCA
CCAAGGGCAAGCGCACCGTCGAGACCGGCATGCGCTCGTTCGAGCACAAGCTGCCCGCCGACACCCCCGAGGACGCACTG
CTGCTGCTGATCGAGAGCCTGAATAAGGACCCCGAGGTGCACGGCATCCTGGTGCAGTTGCCGGTGCCCAAGCACATCGA
TACCCAGAAGGTGATCGAGGCCATCGACCCGGCCAAGGACGTGGACGGCTTCCATCCCGTCAATGTGGGCCGTCTGGCCG
CCGGCGGCGATGCGCTGGTGCCCTGCACGCCGCTGGGCTCGCTGCTGCTGCTCAAGGACACGCTGGGCAAGCTGGGGGGC
CTGGACGCCGTGGTCATCGGCCGCTCCAACATCGTCGGCAAGCCCATGGCCCAATTGCTGATCAAGGAAAGCTGCACGGT
CACCGTCGCCCATTCCCAGACCAAGGACCTGCCCGGCGTGGTGCGTCGCGCCGACATCGTCATCGCCGCGGTGGGGCGGC
CGGAGATGATCAAGGGCGACTGGATTAAGCCGGGCGCCACGGTCATCGATGTGGGCATCAACCGCATCGAGCTGCCCGAC
GGCACCAAGAAGCTGGTGGGCGACGTGGACTTCGCCTCGGCGGTCAAGGTGGCGGGGGCCATCACGCCGGTGCCCGGCGG
CGTCGGTCCCATGACCATCGCCTGCCTGCTTCGCAACACCCTGGTGGCTTGTGCCCGCCTGCACAAACTGCCCGAGGTGG
AGTTCTAG

Upstream 100 bases:

>100_bases
GATATCGTCCTGGGCGCCACCGACCGGCGCAAGGTGATCTTGATTTCCGGCGAGACCGAGGATTTGCGGCATCGCCTCGA
CGAGTGGATGGCAAGCAACC

Downstream 100 bases:

>100_bases
AGCATGATTCGACCCCTTCGAATCGTGCTCGCCGGCCATCGCGGCGGCGAGTGTTCGGCGAGGGTGGGCCGGCAATGACT
TTCACCGGCGGCGTCGGCCA

Product: 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTDAKLIDGNLFSAKLRETITARVAEMKAAHHITPGLAVVLVGEDPASQVYVRTKGKRTVETGMRSFEHKLPADTPEDAL
LLLIESLNKDPEVHGILVQLPVPKHIDTQKVIEAIDPAKDVDGFHPVNVGRLAAGGDALVPCTPLGSLLLLKDTLGKLGG
LDAVVIGRSNIVGKPMAQLLIKESCTVTVAHSQTKDLPGVVRRADIVIAAVGRPEMIKGDWIKPGATVIDVGINRIELPD
GTKKLVGDVDFASAVKVAGAITPVPGGVGPMTIACLLRNTLVACARLHKLPEVEF

Sequences:

>Translated_295_residues
MTDAKLIDGNLFSAKLRETITARVAEMKAAHHITPGLAVVLVGEDPASQVYVRTKGKRTVETGMRSFEHKLPADTPEDAL
LLLIESLNKDPEVHGILVQLPVPKHIDTQKVIEAIDPAKDVDGFHPVNVGRLAAGGDALVPCTPLGSLLLLKDTLGKLGG
LDAVVIGRSNIVGKPMAQLLIKESCTVTVAHSQTKDLPGVVRRADIVIAAVGRPEMIKGDWIKPGATVIDVGINRIELPD
GTKKLVGDVDFASAVKVAGAITPVPGGVGPMTIACLLRNTLVACARLHKLPEVEF
>Mature_294_residues
TDAKLIDGNLFSAKLRETITARVAEMKAAHHITPGLAVVLVGEDPASQVYVRTKGKRTVETGMRSFEHKLPADTPEDALL
LLIESLNKDPEVHGILVQLPVPKHIDTQKVIEAIDPAKDVDGFHPVNVGRLAAGGDALVPCTPLGSLLLLKDTLGKLGGL
DAVVIGRSNIVGKPMAQLLIKESCTVTVAHSQTKDLPGVVRRADIVIAAVGRPEMIKGDWIKPGATVIDVGINRIELPDG
TKKLVGDVDFASAVKVAGAITPVPGGVGPMTIACLLRNTLVACARLHKLPEVEF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=295, Percent_Identity=45.0847457627119, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI94721354, Length=304, Percent_Identity=43.75, Blast_Score=211, Evalue=4e-55,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=42.5675675675676, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI36796743, Length=200, Percent_Identity=27.5, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=53.6842105263158, Blast_Score=274, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=43.9862542955326, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=292, Percent_Identity=45.8904109589041, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=41.0774410774411, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6322933, Length=320, Percent_Identity=27.5, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI62472483, Length=304, Percent_Identity=46.7105263157895, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI45551871, Length=304, Percent_Identity=46.7105263157895, Blast_Score=248, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24645718, Length=304, Percent_Identity=46.7105263157895, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI17137370, Length=304, Percent_Identity=46.7105263157895, Blast_Score=248, Evalue=4e-66,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=48.6394557823129, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=48.6394557823129, Blast_Score=244, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_MAGSA (Q2VYZ7)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_423737.1
- ProteinModelPortal:   Q2VYZ7
- SMR:   Q2VYZ7
- STRING:   Q2VYZ7
- GeneID:   3804411
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb4374
- NMPDR:   fig|342108.5.peg.3930
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLERISP
- PhylomeDB:   Q2VYZ7
- BioCyc:   MMAG342108:AMB4374-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31174; Mature: 31043

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAKLIDGNLFSAKLRETITARVAEMKAAHHITPGLAVVLVGEDPASQVYVRTKGKRTV
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCHHH
ETGMRSFEHKLPADTPEDALLLLIESLNKDPEVHGILVQLPVPKHIDTQKVIEAIDPAKD
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHH
VDGFHPVNVGRLAAGGDALVPCTPLGSLLLLKDTLGKLGGLDAVVIGRSNIVGKPMAQLL
CCCCCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
IKESCTVTVAHSQTKDLPGVVRRADIVIAAVGRPEMIKGDWIKPGATVIDVGINRIELPD
HHCCCEEEEECCCCCCCHHHHHCCCEEEEECCCCCEECCCCCCCCCEEEEECCCEEECCC
GTKKLVGDVDFASAVKVAGAITPVPGGVGPMTIACLLRNTLVACARLHKLPEVEF
CHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDAKLIDGNLFSAKLRETITARVAEMKAAHHITPGLAVVLVGEDPASQVYVRTKGKRTV
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCHHH
ETGMRSFEHKLPADTPEDALLLLIESLNKDPEVHGILVQLPVPKHIDTQKVIEAIDPAKD
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHH
VDGFHPVNVGRLAAGGDALVPCTPLGSLLLLKDTLGKLGGLDAVVIGRSNIVGKPMAQLL
CCCCCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHH
IKESCTVTVAHSQTKDLPGVVRRADIVIAAVGRPEMIKGDWIKPGATVIDVGINRIELPD
HHCCCEEEEECCCCCCCHHHHHCCCEEEEECCCCCEECCCCCCCCCEEEEECCCEEECCC
GTKKLVGDVDFASAVKVAGAITPVPGGVGPMTIACLLRNTLVACARLHKLPEVEF
CHHHHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA