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The map label for this gene is pmbA [H]

Identifier: 83313471

GI number: 83313471

Start: 4776487

End: 4777830

Strand: Reverse

Name: pmbA [H]

Synonym: amb4372

Alternate gene names: 83313471

Gene position: 4777830-4776487 (Counterclockwise)

Preceding gene: 83313472

Following gene: 83313461

Centisome position: 96.19

GC content: 68.3

Gene sequence:

>1344_bases
ATGCCCGGGATTTTGCCCCTCGATCTGCTGAGTGATCTGGTCGCCGATGCCCGCAAGGCCGGCGCCGAAGCCGCCGACGC
GGTGCTGATCGATTCCGCCTCGCTGTCCGTGGGCATGCGCCTGGGCGCGCTGGAGCGCCTGGAGCGGGCCGAATCCGGCG
ACGTGGGGCTGCGCGTGCTGATCGGCAAGCATCAGGCCTTCGTGTCCTCGTCGGACCGTTCCAAGGCGGCCCTGGGCCAA
TTGGTGGAGCGCGCCGTGGCCATGGCCCGCTCGATCCCCGAGGACCCCTATTGCGGCTTGGCCGAGCCCGGTGAACTGGC
CACGTCCTTTCCCGATCTGGATGTCTGTGACCCGCACGAGCCCTCGGCGGAACAGTTGATCGACATGGTCCGCAGTGCCG
AGGACGCTGCCCGTTCCGTGAACGGCGTCACCAATTCCGAGGGCGCCGAGGCGGGCTGGGGGCGGTCGGGCGTGGCCATC
GTCGCCTCCAATGGCTTTTCCCATGCCTATTCGGTGACCTCGTCGTCGCTGTCGGTATCGGTGCTGGCCGGCACCAATGC
GCAGGGCATGGAGCGCGACTACGATTATTCCTCGGCGGTGTTCCTGGCCGATCTGCGTGCGCCGGAGGAGATCGGCCACG
AGGCCGGGCGCCGCGCCGTTCATCGCCTGGGCGCCCGCAAGGTGGCCACCAAGCAGGTGCCGGTGATCTTCGACCCGCGG
GTGGCGCGTGGCCTGGTGTCCAGCTTGGCGGGTGCCATCAATGGCTCGGGGGTGGCACGCGGCACCAGCTTCCTCAAGGA
CAAGCTGGGTCAGGCCATCTTCCCCCGCGGCATCCGCATCATGGATGATCCCCACCGCCGCCGCGGCCTGCGCTCGCGCC
CCTGCGACGGCGAGGGCATCGCCACCCAGCCCCGCGCCATCGTCGAGGACGGCGTGCTCACCACTTGGCTGCTGGACCTG
CGCTCGGCCCGGCAGCTGGGGATGCGGTCCACCGGCCATGCCAGCCGGGGTACCTCGTCGCCACCCTCGCCCTCGGCCAG
CAATTTCTATCTGGAAGCCGGCCACATCACGCCGTCCGAGATGATCCACGACATCTCCGAAGGCTTCTACATCACTGATC
TGTCGGGCCAGGGGGTCAACGGCGTCACCGGCGATTACTCGCGCGGGGCCTCGGGCTTCTGGATCAAGGGAGGCGAGTTG
GCCTTCGCCGTCAACGAAGTGACGGTGGCGGGCAATCTCAAGGAGATGTTCCTCAACCTTACCCCGGCCAATGATCTGAC
GCTGCGCCATGGCATCGACTCGCCGACCCTGCGCATCGACGGGCTGATGGTGGCGGGGCGGTAG

Upstream 100 bases:

>100_bases
GCACCCCATGGTGATCGGACTGAGCCCCCTGCCGTGATCCCCCCTTTACGGCGGCCGCGAATCGGGGCAGCCTGAACGCT
CCCCTGTTGGAGCCGCCGCC

Downstream 100 bases:

>100_bases
GACGTCACGCGGGGCTCCCGCCCCGGTCCCCGGTTGGAGGCGATGCCTCCAAACCTCCCTTCATTTTGAGAATGAAAAGG
GGGCTTGGGGCATCGCCCCA

Product: Zn-dependent protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MPGILPLDLLSDLVADARKAGAEAADAVLIDSASLSVGMRLGALERLERAESGDVGLRVLIGKHQAFVSSSDRSKAALGQ
LVERAVAMARSIPEDPYCGLAEPGELATSFPDLDVCDPHEPSAEQLIDMVRSAEDAARSVNGVTNSEGAEAGWGRSGVAI
VASNGFSHAYSVTSSSLSVSVLAGTNAQGMERDYDYSSAVFLADLRAPEEIGHEAGRRAVHRLGARKVATKQVPVIFDPR
VARGLVSSLAGAINGSGVARGTSFLKDKLGQAIFPRGIRIMDDPHRRRGLRSRPCDGEGIATQPRAIVEDGVLTTWLLDL
RSARQLGMRSTGHASRGTSSPPSPSASNFYLEAGHITPSEMIHDISEGFYITDLSGQGVNGVTGDYSRGASGFWIKGGEL
AFAVNEVTVAGNLKEMFLNLTPANDLTLRHGIDSPTLRIDGLMVAGR

Sequences:

>Translated_447_residues
MPGILPLDLLSDLVADARKAGAEAADAVLIDSASLSVGMRLGALERLERAESGDVGLRVLIGKHQAFVSSSDRSKAALGQ
LVERAVAMARSIPEDPYCGLAEPGELATSFPDLDVCDPHEPSAEQLIDMVRSAEDAARSVNGVTNSEGAEAGWGRSGVAI
VASNGFSHAYSVTSSSLSVSVLAGTNAQGMERDYDYSSAVFLADLRAPEEIGHEAGRRAVHRLGARKVATKQVPVIFDPR
VARGLVSSLAGAINGSGVARGTSFLKDKLGQAIFPRGIRIMDDPHRRRGLRSRPCDGEGIATQPRAIVEDGVLTTWLLDL
RSARQLGMRSTGHASRGTSSPPSPSASNFYLEAGHITPSEMIHDISEGFYITDLSGQGVNGVTGDYSRGASGFWIKGGEL
AFAVNEVTVAGNLKEMFLNLTPANDLTLRHGIDSPTLRIDGLMVAGR
>Mature_446_residues
PGILPLDLLSDLVADARKAGAEAADAVLIDSASLSVGMRLGALERLERAESGDVGLRVLIGKHQAFVSSSDRSKAALGQL
VERAVAMARSIPEDPYCGLAEPGELATSFPDLDVCDPHEPSAEQLIDMVRSAEDAARSVNGVTNSEGAEAGWGRSGVAIV
ASNGFSHAYSVTSSSLSVSVLAGTNAQGMERDYDYSSAVFLADLRAPEEIGHEAGRRAVHRLGARKVATKQVPVIFDPRV
ARGLVSSLAGAINGSGVARGTSFLKDKLGQAIFPRGIRIMDDPHRRRGLRSRPCDGEGIATQPRAIVEDGVLTTWLLDLR
SARQLGMRSTGHASRGTSSPPSPSASNFYLEAGHITPSEMIHDISEGFYITDLSGQGVNGVTGDYSRGASGFWIKGGELA
FAVNEVTVAGNLKEMFLNLTPANDLTLRHGIDSPTLRIDGLMVAGR

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=437, Percent_Identity=37.9862700228833, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1789640, Length=424, Percent_Identity=25.7075471698113, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47083; Mature: 46951

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGILPLDLLSDLVADARKAGAEAADAVLIDSASLSVGMRLGALERLERAESGDVGLRVL
CCCCCHHHHHHHHHHHHHHCCCHHHCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEE
IGKHQAFVSSSDRSKAALGQLVERAVAMARSIPEDPYCGLAEPGELATSFPDLDVCDPHE
EECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
PSAEQLIDMVRSAEDAARSVNGVTNSEGAEAGWGRSGVAIVASNGFSHAYSVTSSSLSVS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEEEE
VLAGTNAQGMERDYDYSSAVFLADLRAPEEIGHEAGRRAVHRLGARKVATKQVPVIFDPR
EEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCH
VARGLVSSLAGAINGSGVARGTSFLKDKLGQAIFPRGIRIMDDPHRRRGLRSRPCDGEGI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHCCCCCEECCCHHHHCCCCCCCCCCCCC
ATQPRAIVEDGVLTTWLLDLRSARQLGMRSTGHASRGTSSPPSPSASNFYLEAGHITPSE
CCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
MIHDISEGFYITDLSGQGVNGVTGDYSRGASGFWIKGGELAFAVNEVTVAGNLKEMFLNL
HHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCEEEEEEEEEEECCCHHHHHEEC
TPANDLTLRHGIDSPTLRIDGLMVAGR
CCCCCCEEECCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
PGILPLDLLSDLVADARKAGAEAADAVLIDSASLSVGMRLGALERLERAESGDVGLRVL
CCCCHHHHHHHHHHHHHHCCCHHHCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEE
IGKHQAFVSSSDRSKAALGQLVERAVAMARSIPEDPYCGLAEPGELATSFPDLDVCDPHE
EECCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCC
PSAEQLIDMVRSAEDAARSVNGVTNSEGAEAGWGRSGVAIVASNGFSHAYSVTSSSLSVS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCEEEE
VLAGTNAQGMERDYDYSSAVFLADLRAPEEIGHEAGRRAVHRLGARKVATKQVPVIFDPR
EEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCH
VARGLVSSLAGAINGSGVARGTSFLKDKLGQAIFPRGIRIMDDPHRRRGLRSRPCDGEGI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHCCCCCEECCCHHHHCCCCCCCCCCCCC
ATQPRAIVEDGVLTTWLLDLRSARQLGMRSTGHASRGTSSPPSPSASNFYLEAGHITPSE
CCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
MIHDISEGFYITDLSGQGVNGVTGDYSRGASGFWIKGGELAFAVNEVTVAGNLKEMFLNL
HHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCEEEEEEEEEEECCCHHHHHEEC
TPANDLTLRHGIDSPTLRIDGLMVAGR
CCCCCCEEECCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]