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The map label for this gene is rlmI [H]

Identifier: 83313461

GI number: 83313461

Start: 4764512

End: 4766974

Strand: Reverse

Name: rlmI [H]

Synonym: amb4362

Alternate gene names: 83313461

Gene position: 4766974-4764512 (Counterclockwise)

Preceding gene: 83313471

Following gene: 83313460

Centisome position: 95.97

GC content: 64.76

Gene sequence:

>2463_bases
GTGCGACAGCGAGTTCACCGCCTTGATCGACATCATCGGACCTTCGAAGGTCGACATGCCGTAGAACGCCACCGAGGAGA
CCAGGAAACGCATGACCGGATCGGTCCGCAGCTTGTCCCAGGCACCCGACAGGGTCATCAGGCCGTTGATCATGCCGCCC
CAAGAGGGCATCCACAGCATCACCGAGAAGGTCATGCCCAGGGTCTGCGCCCAATCGGGCAGAGCGGTGTAGTGCAGGTG
GTGCGGACCGGCCCAGATGTACAGGAAGATCAGCGCCCAGAAGTGCACGATCGACAGACGGTAGGAGTACACCGGACGCT
CGGCCCGCTTCGGCACGAAGTAGTACATGATGCCGAGGAAGCCGGCGGTCAGGAAGAAGCCCACCGCATTGTGGCCGTAC
CACCACTGGGTCATGGCGTTCTGCACGCCACCGAACACGTTGTAGCTCTTCATCCAGCTGCCGCCGAACAGGGCGGCCGG
AACCGCGATGTTGTTGCCGAGGTGCAGCATGGCGATGGTCAGGATCATGGCGAAGAAGAACCAGTTGGCCACATAGATGT
GGGGTTCCTTACGCTTCATGATGGTGCCGGCGAAGGCGATCAGGTAGGCCACCCAAATCACGGTGAGCCACAGGTCGATC
CACCATTCCGGCTCCGCGTATTCCTGGCCCTGGCTGAAGCCCAGAACATAGGACAGAGCGGCGACGACGATCAGCGCCTG
GAAATTCCAGAAAATGAAACCACCAAGGCCGGAACCGCCGAACAGGCTGGCACGGCAGGTCCGCTGCACGATGTACAGAG
AGGTGGAGAAAAGAACGTTACCGCCGAACGCAAAGATCACCGCGGAAGTATGAACGGGACGCAGGCGCCCGAACGAAGTC
CACTCGAGATCGAGGTTCAGAACGGGAAAGGCCAACTGCAGGGCGATGAAGTCGCCCGCCAGGAAGCCCACGACGCCCCA
GAAAATCGCGGCGATCGTGAACTTCTTGATCACGTCGTCGCGATAGGGCGTGGCTTCAGTGTTGATGCTCATGCCGAACT
CCTGTTTCGACCGGAAAAGGGTCCACCTGAAGCATCCGGCCGGATCCATGGCGACACCATGCCCCCGGGGCCGATGGCGG
CCGGGTGGTTCTTTGCTTCAAGTGTGCGCACTATGCCGACGGAAGCACGGGCCGCCATTGACATATATCAAAGTATGGCG
ATTTTCAGGCGTTTCAGGCCCTTGGCGATAATGATTCCCCGCCGTAAAGTGCCGGCCATGACACAGACACATCCAGACCA
GCCCGCCGCCCGTCCCGTGATCCGCCTGGCCAAGGGGCGCTCGAAGCGCCTTCGGGCCGGTCATCCCTGGGTCTTCTCCA
ATGAGATCGAGATGGGGGCCGAGGCCAAGGCCCTGCCGCCCGGCTCTCTCGTCACCCTGATGGATGCCGGGGACGAGCGT
CTGGGCGTCGCCACCTTCAACCCCCATTCCCTGATCGCCGCCCGCGTGCTGTCGCGCCGCGCCGCCGACACCGTGGACGT
GGATTTCCTGGCCGAACGCCTGCGCGCCGCTCTGGCGCTCCGCGACACGTTGTTTCCCACCCCCCATTACCGCCTCGTCC
ATTCCGAGGCCGATCGCCTGCCCGGCCTGATCGTCGACCGCTACGGCGACGTGCTGGCGGTGCAGACTAACACCGCCGGC
ATGGAGCGCCTGCTTCCCGTTCTGCTCGACGCCTTGCAGATGGTTCTCGCCCCCCGCGCCGTGGTGCTGGTCAACGACAG
CCCGGTGCGCAAGCTGGAAGGGCTCGAGCCCCATCACGACGTGGCCTTCGGGCAGGTGGACGGCCCGGTGGAGCTGGAGG
AGAACGGGTGCCGCTTCGTCGCCGATCTGACCGGCGGACAGAAAACCGGCTGGTTCTTCGACCAGCGCGACAACCGCGCC
TTCGTGGCCCGTCTGGCCAAGGGGCGGCGCGTGCTGGACCTCTATACCTATGCCGGCGGCTTCGCGGTGCAGGCGGCCAA
GGCCGGCGCCACCCAAACCATCGCCGTGGACCGGTCGGAACATTCCCTGGCCCTGGCCGATCAGGCGGCACGGCTCAACG
GGGTCGAGGTCCAGACGGTGCGGGCCGAGGCCTTCGCCGAGATGGAGCGCCTGGCCCTGGCGGGAGAGCGTTTCGGGCTG
GTGGTGGCCGATCCGCCGGCCTTCGTCAAGTCGCGCAAGGATCTGGGCGCCGGGGCCAAGGGCTACCGCAAGATGGCCCG
CTTGGCCGCCGCCCTGGTCGAGCCGGGCGGCTTCCTGCTGTGCGCGTCTTGCTCCCACCACATGCCGGTGGAAACCTTCG
CCGAGGAAATCGGCCATGGGCTGCATCTGGCGGGACGGTCCGGCCGCATCCTGCGCAGTGCCGGCGCCGCGTCGGACCAC
CCGGTCCATCCCTGGCTGCCCGAGAGTGCCTATCTTAAAGCCCAAGTTTTCCAATTGGATTGA

Upstream 100 bases:

>100_bases
CCCACAGCTTGGGAACCAGGTAGTACAGAGCGCCGAAGGAAACGAAGGCGACCCAGCCCAGAGCACCGGAGTGCACGTGG
CCAATACCCCAGTCGGTGTA

Downstream 100 bases:

>100_bases
TTTCAAGTCTTCCTGTTGGATTGAATTCCAGCGACCCAAAGCAAATAGCCGACAGACCTAACCTTAAGGCATACAGTAAC
CGTATCAATCGAAATCGGGC

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 820; Mature: 820

Protein sequence:

>820_residues
MRQRVHRLDRHHRTFEGRHAVERHRGDQETHDRIGPQLVPGTRQGHQAVDHAAPRGHPQHHREGHAQGLRPIGQSGVVQV
VRTGPDVQEDQRPEVHDRQTVGVHRTLGPLRHEVVHDAEEAGGQEEAHRIVAVPPLGHGVLHATEHVVALHPAAAEQGGR
NRDVVAEVQHGDGQDHGEEEPVGHIDVGFLTLHDGAGEGDQVGHPNHGEPQVDPPFRLRVFLALAEAQNIGQSGDDDQRL
EIPENETTKAGTAEQAGTAGPLHDVQRGGEKNVTAERKDHRGSMNGTQAPERSPLEIEVQNGKGQLQGDEVARQEAHDAP
ENRGDRELLDHVVAIGRGFSVDAHAELLFRPEKGPPEASGRIHGDTMPPGPMAAGWFFASSVRTMPTEARAAIDIYQSMA
IFRRFRPLAIMIPRRKVPAMTQTHPDQPAARPVIRLAKGRSKRLRAGHPWVFSNEIEMGAEAKALPPGSLVTLMDAGDER
LGVATFNPHSLIAARVLSRRAADTVDVDFLAERLRAALALRDTLFPTPHYRLVHSEADRLPGLIVDRYGDVLAVQTNTAG
MERLLPVLLDALQMVLAPRAVVLVNDSPVRKLEGLEPHHDVAFGQVDGPVELEENGCRFVADLTGGQKTGWFFDQRDNRA
FVARLAKGRRVLDLYTYAGGFAVQAAKAGATQTIAVDRSEHSLALADQAARLNGVEVQTVRAEAFAEMERLALAGERFGL
VVADPPAFVKSRKDLGAGAKGYRKMARLAAALVEPGGFLLCASCSHHMPVETFAEEIGHGLHLAGRSGRILRSAGAASDH
PVHPWLPESAYLKAQVFQLD

Sequences:

>Translated_820_residues
MRQRVHRLDRHHRTFEGRHAVERHRGDQETHDRIGPQLVPGTRQGHQAVDHAAPRGHPQHHREGHAQGLRPIGQSGVVQV
VRTGPDVQEDQRPEVHDRQTVGVHRTLGPLRHEVVHDAEEAGGQEEAHRIVAVPPLGHGVLHATEHVVALHPAAAEQGGR
NRDVVAEVQHGDGQDHGEEEPVGHIDVGFLTLHDGAGEGDQVGHPNHGEPQVDPPFRLRVFLALAEAQNIGQSGDDDQRL
EIPENETTKAGTAEQAGTAGPLHDVQRGGEKNVTAERKDHRGSMNGTQAPERSPLEIEVQNGKGQLQGDEVARQEAHDAP
ENRGDRELLDHVVAIGRGFSVDAHAELLFRPEKGPPEASGRIHGDTMPPGPMAAGWFFASSVRTMPTEARAAIDIYQSMA
IFRRFRPLAIMIPRRKVPAMTQTHPDQPAARPVIRLAKGRSKRLRAGHPWVFSNEIEMGAEAKALPPGSLVTLMDAGDER
LGVATFNPHSLIAARVLSRRAADTVDVDFLAERLRAALALRDTLFPTPHYRLVHSEADRLPGLIVDRYGDVLAVQTNTAG
MERLLPVLLDALQMVLAPRAVVLVNDSPVRKLEGLEPHHDVAFGQVDGPVELEENGCRFVADLTGGQKTGWFFDQRDNRA
FVARLAKGRRVLDLYTYAGGFAVQAAKAGATQTIAVDRSEHSLALADQAARLNGVEVQTVRAEAFAEMERLALAGERFGL
VVADPPAFVKSRKDLGAGAKGYRKMARLAAALVEPGGFLLCASCSHHMPVETFAEEIGHGLHLAGRSGRILRSAGAASDH
PVHPWLPESAYLKAQVFQLD
>Mature_820_residues
MRQRVHRLDRHHRTFEGRHAVERHRGDQETHDRIGPQLVPGTRQGHQAVDHAAPRGHPQHHREGHAQGLRPIGQSGVVQV
VRTGPDVQEDQRPEVHDRQTVGVHRTLGPLRHEVVHDAEEAGGQEEAHRIVAVPPLGHGVLHATEHVVALHPAAAEQGGR
NRDVVAEVQHGDGQDHGEEEPVGHIDVGFLTLHDGAGEGDQVGHPNHGEPQVDPPFRLRVFLALAEAQNIGQSGDDDQRL
EIPENETTKAGTAEQAGTAGPLHDVQRGGEKNVTAERKDHRGSMNGTQAPERSPLEIEVQNGKGQLQGDEVARQEAHDAP
ENRGDRELLDHVVAIGRGFSVDAHAELLFRPEKGPPEASGRIHGDTMPPGPMAAGWFFASSVRTMPTEARAAIDIYQSMA
IFRRFRPLAIMIPRRKVPAMTQTHPDQPAARPVIRLAKGRSKRLRAGHPWVFSNEIEMGAEAKALPPGSLVTLMDAGDER
LGVATFNPHSLIAARVLSRRAADTVDVDFLAERLRAALALRDTLFPTPHYRLVHSEADRLPGLIVDRYGDVLAVQTNTAG
MERLLPVLLDALQMVLAPRAVVLVNDSPVRKLEGLEPHHDVAFGQVDGPVELEENGCRFVADLTGGQKTGWFFDQRDNRA
FVARLAKGRRVLDLYTYAGGFAVQAAKAGATQTIAVDRSEHSLALADQAARLNGVEVQTVRAEAFAEMERLALAGERFGL
VVADPPAFVKSRKDLGAGAKGYRKMARLAAALVEPGGFLLCASCSHHMPVETFAEEIGHGLHLAGRSGRILRSAGAASDH
PVHPWLPESAYLKAQVFQLD

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=385, Percent_Identity=35.3246753246753, Blast_Score=226, Evalue=5e-60,
Organism=Escherichia coli, GI1787181, Length=218, Percent_Identity=29.3577981651376, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 89196; Mature: 89196

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50890 PUA ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRVHRLDRHHRTFEGRHAVERHRGDQETHDRIGPQLVPGTRQGHQAVDHAAPRGHPQH
CCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCEECCCCCCHHHHHHHCCCCCCCHH
HREGHAQGLRPIGQSGVVQVVRTGPDVQEDQRPEVHDRQTVGVHRTLGPLRHEVVHDAEE
HHCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AGGQEEAHRIVAVPPLGHGVLHATEHVVALHPAAAEQGGRNRDVVAEVQHGDGQDHGEEE
CCCHHHCCEEEEECCCCCCHHHHCCCEEEECCHHHHCCCCCCCEEEEEECCCCCCCCCCC
PVGHIDVGFLTLHDGAGEGDQVGHPNHGEPQVDPPFRLRVFLALAEAQNIGQSGDDDQRL
CCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEE
EIPENETTKAGTAEQAGTAGPLHDVQRGGEKNVTAERKDHRGSMNGTQAPERSPLEIEVQ
ECCCCCCCCCCCHHHCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEE
NGKGQLQGDEVARQEAHDAPENRGDRELLDHVVAIGRGFSVDAHAELLFRPEKGPPEASG
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCC
RIHGDTMPPGPMAAGWFFASSVRTMPTEARAAIDIYQSMAIFRRFRPLAIMIPRRKVPAM
EEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
TQTHPDQPAARPVIRLAKGRSKRLRAGHPWVFSNEIEMGAEAKALPPGSLVTLMDAGDER
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCE
LGVATFNPHSLIAARVLSRRAADTVDVDFLAERLRAALALRDTLFPTPHYRLVHSEADRL
EEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHC
PGLIVDRYGDVLAVQTNTAGMERLLPVLLDALQMVLAPRAVVLVNDSPVRKLEGLEPHHD
CCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCC
VAFGQVDGPVELEENGCRFVADLTGGQKTGWFFDQRDNRAFVARLAKGRRVLDLYTYAGG
EEECCCCCCEEECCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHCCCEEEEEEECCCC
FAVQAAKAGATQTIAVDRSEHSLALADQAARLNGVEVQTVRAEAFAEMERLALAGERFGL
EEEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEE
VVADPPAFVKSRKDLGAGAKGYRKMARLAAALVEPGGFLLCASCSHHMPVETFAEEIGHG
EEECCHHHHHCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCC
LHLAGRSGRILRSAGAASDHPVHPWLPESAYLKAQVFQLD
EEEECCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRQRVHRLDRHHRTFEGRHAVERHRGDQETHDRIGPQLVPGTRQGHQAVDHAAPRGHPQH
CCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCEECCCCCCHHHHHHHCCCCCCCHH
HREGHAQGLRPIGQSGVVQVVRTGPDVQEDQRPEVHDRQTVGVHRTLGPLRHEVVHDAEE
HHCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AGGQEEAHRIVAVPPLGHGVLHATEHVVALHPAAAEQGGRNRDVVAEVQHGDGQDHGEEE
CCCHHHCCEEEEECCCCCCHHHHCCCEEEECCHHHHCCCCCCCEEEEEECCCCCCCCCCC
PVGHIDVGFLTLHDGAGEGDQVGHPNHGEPQVDPPFRLRVFLALAEAQNIGQSGDDDQRL
CCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEE
EIPENETTKAGTAEQAGTAGPLHDVQRGGEKNVTAERKDHRGSMNGTQAPERSPLEIEVQ
ECCCCCCCCCCCHHHCCCCCCHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEE
NGKGQLQGDEVARQEAHDAPENRGDRELLDHVVAIGRGFSVDAHAELLFRPEKGPPEASG
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCC
RIHGDTMPPGPMAAGWFFASSVRTMPTEARAAIDIYQSMAIFRRFRPLAIMIPRRKVPAM
EEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCC
TQTHPDQPAARPVIRLAKGRSKRLRAGHPWVFSNEIEMGAEAKALPPGSLVTLMDAGDER
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEECCCCE
LGVATFNPHSLIAARVLSRRAADTVDVDFLAERLRAALALRDTLFPTPHYRLVHSEADRL
EEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHC
PGLIVDRYGDVLAVQTNTAGMERLLPVLLDALQMVLAPRAVVLVNDSPVRKLEGLEPHHD
CCEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCC
VAFGQVDGPVELEENGCRFVADLTGGQKTGWFFDQRDNRAFVARLAKGRRVLDLYTYAGG
EEECCCCCCEEECCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHCCCEEEEEEECCCC
FAVQAAKAGATQTIAVDRSEHSLALADQAARLNGVEVQTVRAEAFAEMERLALAGERFGL
EEEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEE
VVADPPAFVKSRKDLGAGAKGYRKMARLAAALVEPGGFLLCASCSHHMPVETFAEEIGHG
EEECCHHHHHCHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCC
LHLAGRSGRILRSAGAASDHPVHPWLPESAYLKAQVFQLD
EEEECCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA