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The map label for this gene is tam [H]

Identifier: 83313451

GI number: 83313451

Start: 4756303

End: 4757073

Strand: Reverse

Name: tam [H]

Synonym: amb4352

Alternate gene names: 83313451

Gene position: 4757073-4756303 (Counterclockwise)

Preceding gene: 83313458

Following gene: 83313450

Centisome position: 95.77

GC content: 68.22

Gene sequence:

>771_bases
ATGGCCTGGGATCCCGCCATCTATTCCGCCTTTGCCCAGCCGCGCCTGCGTCCGGCGCTCGACCTGATGGCCCGTATCGA
CCTGGCCGACGCCCGCCGGGTGGTGGACCTGGGATGCGGCACCGGCAACGTGACCCGTATTCTTAAGGAACGCTGGGCGG
ACGCCGACGTCATCGGCATCGATTCCTCGCCCGAGATGCTGATGACCGCGCGCGATCACGGCGGCGCCGTGCGCTATCTC
GAGGGCGATGCCGCCGGCTGGGCGGAAAACGGCGGCGAGGTGGACATCCTGTTCTCCAACGCCGCGCTGCATTGGCTGGA
TGGGCACGACAGTCTGTTTCCCAAGCTGATGGAGCGGGTGAGTTCGGGCGGCGTGCTGGCGGTGCAGATGCCCCACAACC
ACTATGCCGCCTCCCACACCATCATGGCCGAGGCCGCCTCTGCCGGGCCGTGGGCCGAGGTGCTCGGCCCCCTGGCCGCC
CGCTTCCCGGTGGGCGAGCCTTCCTTTTATTATGACCTGCTGGCCGCCCATTCCTCCCATATCGACATCTGGGAGACCGA
GTACCTGCACGTGCTGGAAGGCGACAACCCGGTGGTCACCTGGACCAAGGGCACGGCGCTGCGCCCCTTGATGACGGCGG
TGGACGGCGCCTGGGCCACCGGCTTTCTGGCCGAATACGCCCGGCGCATCCAGGCGGCCTACCCGCCGCGTCCCGACGGC
AAGACCCTGTTCCCCTTCCGCCGGCTGTTCATGGTGGCGAGGAAGGACTGA

Upstream 100 bases:

>100_bases
CCCTCGCCGGCGCTCGGGATGACAGGAATAAGCGCTCGCCGGTTTTAGAACCCAGCCCGAACGGCTACACTGGGGCAATT
TTCAAGAGGAGCCGCCCGAC

Downstream 100 bases:

>100_bases
CGCCGCCGATTGAACCGTTGCCGATCACGCCCAGTGTTGCGCCTCTCACAGATCGGCGCCACCAATCCCTGTAGATAATG
GTCATGGCGCCATTAAAGGG

Product: trans-aconitate methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MAWDPAIYSAFAQPRLRPALDLMARIDLADARRVVDLGCGTGNVTRILKERWADADVIGIDSSPEMLMTARDHGGAVRYL
EGDAAGWAENGGEVDILFSNAALHWLDGHDSLFPKLMERVSSGGVLAVQMPHNHYAASHTIMAEAASAGPWAEVLGPLAA
RFPVGEPSFYYDLLAAHSSHIDIWETEYLHVLEGDNPVVTWTKGTALRPLMTAVDGAWATGFLAEYARRIQAAYPPRPDG
KTLFPFRRLFMVARKD

Sequences:

>Translated_256_residues
MAWDPAIYSAFAQPRLRPALDLMARIDLADARRVVDLGCGTGNVTRILKERWADADVIGIDSSPEMLMTARDHGGAVRYL
EGDAAGWAENGGEVDILFSNAALHWLDGHDSLFPKLMERVSSGGVLAVQMPHNHYAASHTIMAEAASAGPWAEVLGPLAA
RFPVGEPSFYYDLLAAHSSHIDIWETEYLHVLEGDNPVVTWTKGTALRPLMTAVDGAWATGFLAEYARRIQAAYPPRPDG
KTLFPFRRLFMVARKD
>Mature_255_residues
AWDPAIYSAFAQPRLRPALDLMARIDLADARRVVDLGCGTGNVTRILKERWADADVIGIDSSPEMLMTARDHGGAVRYLE
GDAAGWAENGGEVDILFSNAALHWLDGHDSLFPKLMERVSSGGVLAVQMPHNHYAASHTIMAEAASAGPWAEVLGPLAAR
FPVGEPSFYYDLLAAHSSHIDIWETEYLHVLEGDNPVVTWTKGTALRPLMTAVDGAWATGFLAEYARRIQAAYPPRPDGK
TLFPFRRLFMVARKD

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=253, Percent_Identity=37.9446640316205, Blast_Score=184, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 28085; Mature: 27954

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWDPAIYSAFAQPRLRPALDLMARIDLADARRVVDLGCGTGNVTRILKERWADADVIGI
CCCCHHHHHHHCCCCCHHHHHHHHHCCHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEC
DSSPEMLMTARDHGGAVRYLEGDAAGWAENGGEVDILFSNAALHWLDGHDSLFPKLMERV
CCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHH
SSGGVLAVQMPHNHYAASHTIMAEAASAGPWAEVLGPLAARFPVGEPSFYYDLLAAHSSH
CCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
IDIWETEYLHVLEGDNPVVTWTKGTALRPLMTAVDGAWATGFLAEYARRIQAAYPPRPDG
CEEECCCEEEEEECCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
KTLFPFRRLFMVARKD
CEEHHHHHHHHHHCCC
>Mature Secondary Structure 
AWDPAIYSAFAQPRLRPALDLMARIDLADARRVVDLGCGTGNVTRILKERWADADVIGI
CCCHHHHHHHCCCCCHHHHHHHHHCCHHHHHEEEEECCCCCHHHHHHHHHCCCCEEEEC
DSSPEMLMTARDHGGAVRYLEGDAAGWAENGGEVDILFSNAALHWLDGHDSLFPKLMERV
CCCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHH
SSGGVLAVQMPHNHYAASHTIMAEAASAGPWAEVLGPLAARFPVGEPSFYYDLLAAHSSH
CCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
IDIWETEYLHVLEGDNPVVTWTKGTALRPLMTAVDGAWATGFLAEYARRIQAAYPPRPDG
CEEECCCEEEEEECCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCC
KTLFPFRRLFMVARKD
CEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA