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The map label for this gene is gloB [H]

Identifier: 83313291

GI number: 83313291

Start: 4607047

End: 4607859

Strand: Reverse

Name: gloB [H]

Synonym: amb4192

Alternate gene names: 83313291

Gene position: 4607859-4607047 (Counterclockwise)

Preceding gene: 83313293

Following gene: 83313290

Centisome position: 92.77

GC content: 68.76

Gene sequence:

>813_bases
TTGATGCTACATTCGGCCTATCGCTGCGGGTGGATGTCCATGGCCAAGATCATCGTCGAGCAGATTCCGGTATTGTCCGA
CAATTACGTCTATCTGGCGCACGAGCCCCTGGGCGGGGCCACGGCCGCCATCGATCCGGCGGTGTCCGAGCCGGTGCTGG
AACGTCTGGCCGCCCGGGGCTGGACGCTGACCCATATCCTCAACACCCATCATCACGGCGACCACACCGGGGCCAATCTG
GATCTGGCGCGGCGCACGGGCTGCGCCGTGGTCGGAGCGGCCCGCGATTCCGAGCGCATCCCCGGCATCACCCTGGAGGT
GTCCGAGGGCGAGACCTTCATGCTGGGCCATGCCGCCGTCACCGTCCTCGAGGTTCCAGGCCATACCTCGGGGCATGTGG
CCTACTGGCTGGCCGACAGCCATGTGCTGTTTTGCGGCGACACGCTGTTCTCGCTGGGCTGCGGTCGGCTGTTCGAGGGC
ACGGCCGAGGAGATGTGGGCCTCGCTGAAGAAGCTGCGCGACCTGCCGCCGGACACGCTGGCCTATCCCGCCCACGAATA
TACCGCCGGCAACGGCCGCTTCGCCCGGCTGGTGGAGCGCGACAACGGGGCGCTGAAGGCCCGGCTGGACGAGGTGGAGC
GCCTGCGCGCCCACAACCGTCCCACCGTGCCGGTGCGGCTGGCCGACGAGCGTGCCGCCAATCCCTTCCTGCGGGCCGAC
ACCGCCGCGATCGCCCGGGCGGTGGGGCTGGAGCCGGGAACCGATCCCGCTTTGGTCTTCGCCGAATTGCGGCGGCGCAA
GGACGTGTTCTGA

Upstream 100 bases:

>100_bases
AGGCTGCTGCCGTAAAAATCGCGCAGATCGACCACATCGGTCCACATGGCGGGATAGGACAGGTCTCGATTCATGGCGCA
AGGATAGGCGTTTCGGCCGC

Downstream 100 bases:

>100_bases
CCGGAAAGCCGGGGCCGACCGGCCCCTTTGTGAACAACAGGGAGTGAGCCATGGGGCACGGCCGTGTTCTATTCGTCGAC
GACGAGCCCAGGGTGCTCGA

Product: Zn-dependent hydrolase, including glyoxylase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MMLHSAYRCGWMSMAKIIVEQIPVLSDNYVYLAHEPLGGATAAIDPAVSEPVLERLAARGWTLTHILNTHHHGDHTGANL
DLARRTGCAVVGAARDSERIPGITLEVSEGETFMLGHAAVTVLEVPGHTSGHVAYWLADSHVLFCGDTLFSLGCGRLFEG
TAEEMWASLKKLRDLPPDTLAYPAHEYTAGNGRFARLVERDNGALKARLDEVERLRAHNRPTVPVRLADERAANPFLRAD
TAAIARAVGLEPGTDPALVFAELRRRKDVF

Sequences:

>Translated_270_residues
MMLHSAYRCGWMSMAKIIVEQIPVLSDNYVYLAHEPLGGATAAIDPAVSEPVLERLAARGWTLTHILNTHHHGDHTGANL
DLARRTGCAVVGAARDSERIPGITLEVSEGETFMLGHAAVTVLEVPGHTSGHVAYWLADSHVLFCGDTLFSLGCGRLFEG
TAEEMWASLKKLRDLPPDTLAYPAHEYTAGNGRFARLVERDNGALKARLDEVERLRAHNRPTVPVRLADERAANPFLRAD
TAAIARAVGLEPGTDPALVFAELRRRKDVF
>Mature_270_residues
MMLHSAYRCGWMSMAKIIVEQIPVLSDNYVYLAHEPLGGATAAIDPAVSEPVLERLAARGWTLTHILNTHHHGDHTGANL
DLARRTGCAVVGAARDSERIPGITLEVSEGETFMLGHAAVTVLEVPGHTSGHVAYWLADSHVLFCGDTLFSLGCGRLFEG
TAEEMWASLKKLRDLPPDTLAYPAHEYTAGNGRFARLVERDNGALKARLDEVERLRAHNRPTVPVRLADERAANPFLRAD
TAAIARAVGLEPGTDPALVFAELRRRKDVF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=259, Percent_Identity=38.6100386100386, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI94538322, Length=259, Percent_Identity=38.6100386100386, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI116642887, Length=265, Percent_Identity=40, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI21703352, Length=265, Percent_Identity=40, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI14150041, Length=237, Percent_Identity=41.7721518987342, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=43.4343434343434, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI41327741, Length=185, Percent_Identity=34.0540540540541, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786406, Length=254, Percent_Identity=41.7322834645669, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787158, Length=166, Percent_Identity=31.3253012048193, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536925, Length=264, Percent_Identity=35.2272727272727, Blast_Score=138, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324614, Length=261, Percent_Identity=34.4827586206897, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320478, Length=261, Percent_Identity=32.5670498084291, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=37.3076923076923, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=37.3076923076923, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=37.3076923076923, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI221330176, Length=214, Percent_Identity=28.0373831775701, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001018
- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29472; Mature: 29472

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLHSAYRCGWMSMAKIIVEQIPVLSDNYVYLAHEPLGGATAAIDPAVSEPVLERLAARG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHCC
WTLTHILNTHHHGDHTGANLDLARRTGCAVVGAARDSERIPGITLEVSEGETFMLGHAAV
CEEEEHHHCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEEEEE
TVLEVPGHTSGHVAYWLADSHVLFCGDTLFSLGCGRLFEGTAEEMWASLKKLRDLPPDTL
EEEECCCCCCCEEEEEEECCEEEEECHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCHH
AYPAHEYTAGNGRFARLVERDNGALKARLDEVERLRAHNRPTVPVRLADERAANPFLRAD
CCCCHHEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHH
TAAIARAVGLEPGTDPALVFAELRRRKDVF
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMLHSAYRCGWMSMAKIIVEQIPVLSDNYVYLAHEPLGGATAAIDPAVSEPVLERLAARG
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHCC
WTLTHILNTHHHGDHTGANLDLARRTGCAVVGAARDSERIPGITLEVSEGETFMLGHAAV
CEEEEHHHCCCCCCCCCCCCCHHHCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEEEEE
TVLEVPGHTSGHVAYWLADSHVLFCGDTLFSLGCGRLFEGTAEEMWASLKKLRDLPPDTL
EEEECCCCCCCEEEEEEECCEEEEECHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCHH
AYPAHEYTAGNGRFARLVERDNGALKARLDEVERLRAHNRPTVPVRLADERAANPFLRAD
CCCCHHEECCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHH
TAAIARAVGLEPGTDPALVFAELRRRKDVF
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA