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The map label for this gene is rpfG [H]

Identifier: 83313243

GI number: 83313243

Start: 4549506

End: 4550651

Strand: Reverse

Name: rpfG [H]

Synonym: amb4144

Alternate gene names: 83313243

Gene position: 4550651-4549506 (Counterclockwise)

Preceding gene: 83313244

Following gene: 83313241

Centisome position: 91.61

GC content: 64.92

Gene sequence:

>1146_bases
ATGAGCGACGACATCCGCCCGACCATCCTGGTGGTCGACGACACGCCCGACAACCTGAAGCTGATGAGCGGGCTGCTGAA
GGACCTCTACCGGGTCAAGATCGCCAACGGCGGCGAGAAGGCGCTGGCCATCGCGGCGTCCGATGCGCCGCCCGACCTGA
TCCTGCTGGACATCATGATGCCCGAGGTCGACGGCTACGAGGTGTGCAGGCGCCTCAAGGCCAGCAGAGCAACCCGCGAC
ATCCCGGTGATCTTTCTCACCGCCATGAGCGGCAGCGAGGACGAGGAGGTGGGGCTCAAGATGGGGGCGGTGGACTACAT
CACCAAGCCCATCACTCCGGCCGTGGTGCTGGCCCGGGTCGAGACCCACCTGAAGCTCAAGGCCAGCGCCGATTTCCTGC
GCGACAAGACCGCGTATCTGGAGACCGAGGTGACGCGCCGTACCCGGGAACTGGCCGCCATTCAGGACGTGACGATTCTG
GCCATGGCCTCCCTGGCCGAGACGCGGGATTCCGACACCGGCAACCATATCCGGCGGACCCAGTACTATGTCCGTGCCCT
GGCCCTGAAACTCAGGGACCACCCTCGTTTCTCATCCTCGCTTGACGACGGCGTCGTCGACATGCTGTTCAAATCGGCGC
CGCTGCACGACATCGGCAAGGTCGGCATTCCTGACCGCATCCTGCTGAAGCCGGGACGGTTCGAGCCCGCCGAATTCGAG
ATCATGAAGACCCACACCACCCTGGGACGCGATGCCATCGAGCACGCCGAGCAATCCCTGGGCACGCCGGTGGCCTTTTT
GTCCATGGCCAAGGAAATCGCCTATGCCCACCAGGAAAAGTGGGACGGCAGCGGCTACCCCCGGGGGATTGGCGGCGACG
ACATCCCTGTCTCCGCCCGCCTGATGGCGGTGGCCGACGTCTACGACGCCCTGATCAGCCGCCGGGTCTACAAGGAGGGC
ATGCCCCACGCCACGGCGGTGCAGATCATCCGCGAGGGCAGCGGTTCCCATTTCGACCCCGATATCGTCGAGGCCTTTCT
CGCCCTGGAGGACGAGTTCAAGGCCATCGCCGCCCGCTTCGCCGACAGCGACGGCGACATGGAAAAAAAGAAGGAATATC
TCAGCCGGGCGGGGGGCGTTCCGTGA

Upstream 100 bases:

>100_bases
CTGGTCAAGCCGGTCTCCATCGCCGCCGTCAAGAAATCCATCGAGCGCGCCCTGAAGGTGACGCTGCCATGAGCCGCCCG
GCGATTGAGGGGAATGAAGC

Downstream 100 bases:

>100_bases
GGCGGGCCTAACATCGGATGGTCGGCGTATTACCCGTATCGGGACGCGCCGACTCTCCCTCATAACGCCTGACTTCCCCG
GTAATGGCGCTGTCGGCGAT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSDDIRPTILVVDDTPDNLKLMSGLLKDLYRVKIANGGEKALAIAASDAPPDLILLDIMMPEVDGYEVCRRLKASRATRD
IPVIFLTAMSGSEDEEVGLKMGAVDYITKPITPAVVLARVETHLKLKASADFLRDKTAYLETEVTRRTRELAAIQDVTIL
AMASLAETRDSDTGNHIRRTQYYVRALALKLRDHPRFSSSLDDGVVDMLFKSAPLHDIGKVGIPDRILLKPGRFEPAEFE
IMKTHTTLGRDAIEHAEQSLGTPVAFLSMAKEIAYAHQEKWDGSGYPRGIGGDDIPVSARLMAVADVYDALISRRVYKEG
MPHATAVQIIREGSGSHFDPDIVEAFLALEDEFKAIAARFADSDGDMEKKKEYLSRAGGVP

Sequences:

>Translated_381_residues
MSDDIRPTILVVDDTPDNLKLMSGLLKDLYRVKIANGGEKALAIAASDAPPDLILLDIMMPEVDGYEVCRRLKASRATRD
IPVIFLTAMSGSEDEEVGLKMGAVDYITKPITPAVVLARVETHLKLKASADFLRDKTAYLETEVTRRTRELAAIQDVTIL
AMASLAETRDSDTGNHIRRTQYYVRALALKLRDHPRFSSSLDDGVVDMLFKSAPLHDIGKVGIPDRILLKPGRFEPAEFE
IMKTHTTLGRDAIEHAEQSLGTPVAFLSMAKEIAYAHQEKWDGSGYPRGIGGDDIPVSARLMAVADVYDALISRRVYKEG
MPHATAVQIIREGSGSHFDPDIVEAFLALEDEFKAIAARFADSDGDMEKKKEYLSRAGGVP
>Mature_380_residues
SDDIRPTILVVDDTPDNLKLMSGLLKDLYRVKIANGGEKALAIAASDAPPDLILLDIMMPEVDGYEVCRRLKASRATRDI
PVIFLTAMSGSEDEEVGLKMGAVDYITKPITPAVVLARVETHLKLKASADFLRDKTAYLETEVTRRTRELAAIQDVTILA
MASLAETRDSDTGNHIRRTQYYVRALALKLRDHPRFSSSLDDGVVDMLFKSAPLHDIGKVGIPDRILLKPGRFEPAEFEI
MKTHTTLGRDAIEHAEQSLGTPVAFLSMAKEIAYAHQEKWDGSGYPRGIGGDDIPVSARLMAVADVYDALISRRVYKEGM
PHATAVQIIREGSGSHFDPDIVEAFLALEDEFKAIAARFADSDGDMEKKKEYLSRAGGVP

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=124, Percent_Identity=39.5161290322581, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=39.0476190476191, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=37.3983739837398, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786599, Length=84, Percent_Identity=45.2380952380952, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786784, Length=122, Percent_Identity=35.2459016393443, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789809, Length=125, Percent_Identity=34.4, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=34.7457627118644, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=38.4615384615385, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693157, Length=117, Percent_Identity=35.8974358974359, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=105, Percent_Identity=39.0476190476191, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41926; Mature: 41794

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDIRPTILVVDDTPDNLKLMSGLLKDLYRVKIANGGEKALAIAASDAPPDLILLDIMM
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEC
PEVDGYEVCRRLKASRATRDIPVIFLTAMSGSEDEEVGLKMGAVDYITKPITPAVVLARV
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHECCHHHHHHCCCCHHHHHHHH
ETHLKLKASADFLRDKTAYLETEVTRRTRELAAIQDVTILAMASLAETRDSDTGNHIRRT
HHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QYYVRALALKLRDHPRFSSSLDDGVVDMLFKSAPLHDIGKVGIPDRILLKPGRFEPAEFE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCCCEE
IMKTHTTLGRDAIEHAEQSLGTPVAFLSMAKEIAYAHQEKWDGSGYPRGIGGDDIPVSAR
EEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
LMAVADVYDALISRRVYKEGMPHATAVQIIREGSGSHFDPDIVEAFLALEDEFKAIAARF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ADSDGDMEKKKEYLSRAGGVP
CCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDDIRPTILVVDDTPDNLKLMSGLLKDLYRVKIANGGEKALAIAASDAPPDLILLDIMM
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEC
PEVDGYEVCRRLKASRATRDIPVIFLTAMSGSEDEEVGLKMGAVDYITKPITPAVVLARV
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHECCHHHHHHCCCCHHHHHHHH
ETHLKLKASADFLRDKTAYLETEVTRRTRELAAIQDVTILAMASLAETRDSDTGNHIRRT
HHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QYYVRALALKLRDHPRFSSSLDDGVVDMLFKSAPLHDIGKVGIPDRILLKPGRFEPAEFE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCCCCEE
IMKTHTTLGRDAIEHAEQSLGTPVAFLSMAKEIAYAHQEKWDGSGYPRGIGGDDIPVSAR
EEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
LMAVADVYDALISRRVYKEGMPHATAVQIIREGSGSHFDPDIVEAFLALEDEFKAIAARF
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ADSDGDMEKKKEYLSRAGGVP
CCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]