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The map label for this gene is nadB [H]

Identifier: 83312983

GI number: 83312983

Start: 4291235

End: 4292854

Strand: Reverse

Name: nadB [H]

Synonym: amb3884

Alternate gene names: 83312983

Gene position: 4292854-4291235 (Counterclockwise)

Preceding gene: 83312984

Following gene: 83312982

Centisome position: 86.42

GC content: 68.15

Gene sequence:

>1620_bases
ATGAGCAAGGCGGCGAATCCTTACGATCTGCTGGTCATCGGCTCGGGGGCCGCCGGCCTCTCGGTGGCGCTGGGCGTGCT
GGCGGCCAATCCCAAGGCCCGGGTGGGGGTGCTGGCCAAGACCGTCCTGTCCGAGGGCAGCACCCGCTATGCCCAGGGCG
GCATCGCCGCGGTGCTGGACGCCGCCGATTCCACCGAATCCCACATCGCCGACACCCTGGTGGCCGGGGCGGGCATCTGC
GTCGAATCGGCGGTGCGGGCCGTGGTGGAAGGCGCCAGGGAGGCCATCGAATGGCTGGTGGCCAATGGAGTGGCCTTTGA
CCGCGAGGCGGGCGAGCTTCACCTTACCCGCGAGGGCGGCCACTCCCACCGTCGCATCGTCCATTCCGCCGACGCCACCG
GCAAGGCGGTGGAAGACGCGCTGGAAAGCCGCATCCGGGAAAGCGGCGCCGATCTGTTCGAGCATCACAATGCCGTCGAT
CTGATCCGCGAGAAGCTGCCCCAGGGGCGCCTGGGGCGCTGCATCGGCGCCTACGCCCTGGACCGCCGCGACGGTCGGGT
CCATCGCTTCGCCGCCCGCAATGTGGTGCTGGCCACCGGCGGCGCAAGCCGCGTCTATCTCTATTCCTCCAATCCCGACG
GCTCCACGGGCGACGGCATCGCCATGGCCTGGCGCGCCGGCTGCCGTGTCGCCAACATGGAATTCAGCCAGTTTCACCCC
ACCTGCCTGTTTCACCCCAAGGCCAAGTCCTTCCTGGTGACCGAGGCGGTGCGGGGCGAGGGCGGCCTGCTGCTGCGTCC
CGACGGCACCCGCTTCATGGACGATTACGATCCCAGGGGTGAACTGGCGCCGCGCGACATCGTGGCGCGGGCCATCGACG
ACGTGATGAAGCGCCTGGGCTGCGAGTGCGTCTATCTCGACATCAGCCACAAGCCGGCCGAGTTCGTGAAGGCCCACTTT
CCCACGGTCCATGCCGAGGCGCTGAAGTTCGGCATCGACATGACCAAGGAGCCGCTGCCGGTGGTGCCGGCCGCCCATTA
CACCTGTGGCGGCGTGCTCACCGATCTGGCCGGGCGCACCGACGTGGACAATCTTTACGCGGTGGGCGAGGTGGCCTTCA
CCGGCCTGCACGGCGCCAACCGCATGGCCTCCAATTCCCTGCTGGAATGCCTGGTGCTGGGCGCCGCGGCGGCCCGCGAC
ATCGCGGCCCGAATCGGCACCCTGTCGGATATTCCCGAGATCGCGCCCTGGGACGAGACCTGGGTCACCGATTCCGACGA
AGAGGTGGTGGTCGCCCATAACTGGGACGAGTTGCGCCGCTTCATGTGGGACTATGTGGGCATCGTGCGCTCCACCAAGC
GCCTGGAGCGGGCGCGGCACCGGGTCCGGCTGCTGAAGGACGAAATCGACGAGTATTACGGCAGCTTCCGCGTCACCTCC
GACCTGCTGGAGCTGCGCAACCTGTCCATGGTGGCCGATCTGATCATCCGTTCGGCCCTGGCCCGGCGCGAAAGCCGGGG
GCTGCACTTCACCATCGATTTCCCGGCGCCCGACACCATCGCGCCGCCCCAGAACACCATCCTGGTGCCCAAGAACTTCT
CCATCCGCCGCGTCGACTGA

Upstream 100 bases:

>100_bases
ACCATCCGCGCCATCGCCGAGACCGGAGTCGACTTCGTCTCGGTGGGACGCATCACCCAATCGGCCCCGGCGGTCGATAT
CGGGCTGGACTGGAGCTGAG

Downstream 100 bases:

>100_bases
AACGCCAGAGGCCGCCCATGAACAACTCCGCCCGCCGCGTCGTTTTCCCCAAGGGCGCCACCGTCTTCGCCGAGGGCGAG
CCGGGCACCACCGCCTATGT

Product: aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSKAANPYDLLVIGSGAAGLSVALGVLAANPKARVGVLAKTVLSEGSTRYAQGGIAAVLDAADSTESHIADTLVAGAGIC
VESAVRAVVEGAREAIEWLVANGVAFDREAGELHLTREGGHSHRRIVHSADATGKAVEDALESRIRESGADLFEHHNAVD
LIREKLPQGRLGRCIGAYALDRRDGRVHRFAARNVVLATGGASRVYLYSSNPDGSTGDGIAMAWRAGCRVANMEFSQFHP
TCLFHPKAKSFLVTEAVRGEGGLLLRPDGTRFMDDYDPRGELAPRDIVARAIDDVMKRLGCECVYLDISHKPAEFVKAHF
PTVHAEALKFGIDMTKEPLPVVPAAHYTCGGVLTDLAGRTDVDNLYAVGEVAFTGLHGANRMASNSLLECLVLGAAAARD
IAARIGTLSDIPEIAPWDETWVTDSDEEVVVAHNWDELRRFMWDYVGIVRSTKRLERARHRVRLLKDEIDEYYGSFRVTS
DLLELRNLSMVADLIIRSALARRESRGLHFTIDFPAPDTIAPPQNTILVPKNFSIRRVD

Sequences:

>Translated_539_residues
MSKAANPYDLLVIGSGAAGLSVALGVLAANPKARVGVLAKTVLSEGSTRYAQGGIAAVLDAADSTESHIADTLVAGAGIC
VESAVRAVVEGAREAIEWLVANGVAFDREAGELHLTREGGHSHRRIVHSADATGKAVEDALESRIRESGADLFEHHNAVD
LIREKLPQGRLGRCIGAYALDRRDGRVHRFAARNVVLATGGASRVYLYSSNPDGSTGDGIAMAWRAGCRVANMEFSQFHP
TCLFHPKAKSFLVTEAVRGEGGLLLRPDGTRFMDDYDPRGELAPRDIVARAIDDVMKRLGCECVYLDISHKPAEFVKAHF
PTVHAEALKFGIDMTKEPLPVVPAAHYTCGGVLTDLAGRTDVDNLYAVGEVAFTGLHGANRMASNSLLECLVLGAAAARD
IAARIGTLSDIPEIAPWDETWVTDSDEEVVVAHNWDELRRFMWDYVGIVRSTKRLERARHRVRLLKDEIDEYYGSFRVTS
DLLELRNLSMVADLIIRSALARRESRGLHFTIDFPAPDTIAPPQNTILVPKNFSIRRVD
>Mature_538_residues
SKAANPYDLLVIGSGAAGLSVALGVLAANPKARVGVLAKTVLSEGSTRYAQGGIAAVLDAADSTESHIADTLVAGAGICV
ESAVRAVVEGAREAIEWLVANGVAFDREAGELHLTREGGHSHRRIVHSADATGKAVEDALESRIRESGADLFEHHNAVDL
IREKLPQGRLGRCIGAYALDRRDGRVHRFAARNVVLATGGASRVYLYSSNPDGSTGDGIAMAWRAGCRVANMEFSQFHPT
CLFHPKAKSFLVTEAVRGEGGLLLRPDGTRFMDDYDPRGELAPRDIVARAIDDVMKRLGCECVYLDISHKPAEFVKAHFP
TVHAEALKFGIDMTKEPLPVVPAAHYTCGGVLTDLAGRTDVDNLYAVGEVAFTGLHGANRMASNSLLECLVLGAAAARDI
AARIGTLSDIPEIAPWDETWVTDSDEEVVVAHNWDELRRFMWDYVGIVRSTKRLERARHRVRLLKDEIDEYYGSFRVTSD
LLELRNLSMVADLIIRSALARRESRGLHFTIDFPAPDTIAPPQNTILVPKNFSIRRVD

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=527, Percent_Identity=33.0170777988615, Blast_Score=225, Evalue=9e-59,
Organism=Escherichia coli, GI1788928, Length=538, Percent_Identity=60.4089219330855, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=519, Percent_Identity=31.5992292870906, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786942, Length=549, Percent_Identity=32.0582877959927, Blast_Score=199, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17550100, Length=524, Percent_Identity=33.0152671755725, Blast_Score=229, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17505833, Length=532, Percent_Identity=32.7067669172932, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71986328, Length=432, Percent_Identity=27.5462962962963, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322701, Length=553, Percent_Identity=30.0180831826401, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6322416, Length=549, Percent_Identity=31.1475409836066, Blast_Score=210, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6320788, Length=484, Percent_Identity=28.099173553719, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322511, Length=473, Percent_Identity=24.5243128964059, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17137288, Length=526, Percent_Identity=32.5095057034221, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24655642, Length=526, Percent_Identity=32.5095057034221, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24655647, Length=526, Percent_Identity=32.5095057034221, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI24663005, Length=524, Percent_Identity=31.8702290076336, Blast_Score=215, Evalue=7e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 58573; Mature: 58442

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAANPYDLLVIGSGAAGLSVALGVLAANPKARVGVLAKTVLSEGSTRYAQGGIAAVLD
CCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCEEEEE
AADSTESHIADTLVAGAGICVESAVRAVVEGAREAIEWLVANGVAFDREAGELHLTREGG
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCC
HSHRRIVHSADATGKAVEDALESRIRESGADLFEHHNAVDLIREKLPQGRLGRCIGAYAL
CCHHHEEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHH
DRRDGRVHRFAARNVVLATGGASRVYLYSSNPDGSTGDGIAMAWRAGCRVANMEFSQFHP
CCCCCCEEEEECCEEEEEECCCCEEEEEECCCCCCCCCCEEHHHHCCCEEECCCHHHCCC
TCLFHPKAKSFLVTEAVRGEGGLLLRPDGTRFMDDYDPRGELAPRDIVARAIDDVMKRLG
EEEECCCCCCEEEEHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
CECVYLDISHKPAEFVKAHFPTVHAEALKFGIDMTKEPLPVVPAAHYTCGGVLTDLAGRT
CEEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
DVDNLYAVGEVAFTGLHGANRMASNSLLECLVLGAAAARDIAARIGTLSDIPEIAPWDET
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCC
WVTDSDEEVVVAHNWDELRRFMWDYVGIVRSTKRLERARHRVRLLKDEIDEYYGSFRVTS
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
DLLELRNLSMVADLIIRSALARRESRGLHFTIDFPAPDTIAPPQNTILVPKNFSIRRVD
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEECC
>Mature Secondary Structure 
SKAANPYDLLVIGSGAAGLSVALGVLAANPKARVGVLAKTVLSEGSTRYAQGGIAAVLD
CCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCEEEEE
AADSTESHIADTLVAGAGICVESAVRAVVEGAREAIEWLVANGVAFDREAGELHLTREGG
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCC
HSHRRIVHSADATGKAVEDALESRIRESGADLFEHHNAVDLIREKLPQGRLGRCIGAYAL
CCHHHEEECCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHH
DRRDGRVHRFAARNVVLATGGASRVYLYSSNPDGSTGDGIAMAWRAGCRVANMEFSQFHP
CCCCCCEEEEECCEEEEEECCCCEEEEEECCCCCCCCCCEEHHHHCCCEEECCCHHHCCC
TCLFHPKAKSFLVTEAVRGEGGLLLRPDGTRFMDDYDPRGELAPRDIVARAIDDVMKRLG
EEEECCCCCCEEEEHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
CECVYLDISHKPAEFVKAHFPTVHAEALKFGIDMTKEPLPVVPAAHYTCGGVLTDLAGRT
CEEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
DVDNLYAVGEVAFTGLHGANRMASNSLLECLVLGAAAARDIAARIGTLSDIPEIAPWDET
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCC
WVTDSDEEVVVAHNWDELRRFMWDYVGIVRSTKRLERARHRVRLLKDEIDEYYGSFRVTS
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
DLLELRNLSMVADLIIRSALARRESRGLHFTIDFPAPDTIAPPQNTILVPKNFSIRRVD
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]