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The map label for this gene is ytcI [H]

Identifier: 83312914

GI number: 83312914

Start: 4213800

End: 4215449

Strand: Reverse

Name: ytcI [H]

Synonym: amb3815

Alternate gene names: 83312914

Gene position: 4215449-4213800 (Counterclockwise)

Preceding gene: 83312915

Following gene: 83312913

Centisome position: 84.87

GC content: 73.15

Gene sequence:

>1650_bases
ATGCTGACCGCCTGCCGTTCCTATGAAGAAGCCTGCCGGACCTTTCGCTGGAAGATTCCGGAGCGCTACAACTTGGCCTT
CGACGTCTGCGACCGCCAGACCATGGCCGGGGCCGACGGCCATCGCACCGCCCTGATCGTCGAGGCCGCCGATGGCGGGG
TCGAGCGCCACACCTTCCACATGCTGCGCCTGCTGTCCAACCGGCTGGCCAACGGGCTGGCCGCCATGGGCATCGCTCCC
GGCGACCGGGTAGCCATGGCGCTGCCCGCCGGGCTGGAGGCGGCGGTTACCCTGCTGGCGGTCACAAAGATGGGCGCCGT
GCTGGTGCCCGTGCCGCTGTCCCTGGGCTCGGAGCCCCTGGGCTGGCGGCTGGCCGACAGCGGCGCCAGGGTGGCGGTGG
TCGATCCGGCGGCGCTGCCGGCGCTGGAGCGCGCCCGGTCCCGCCTGCCCGAGCTGGAGCGGGTGCTGGCGGTGGGCGGC
GGCGGCGGTGCCCTGGACCTGTGGTCCGAGCTGGAGCGCAGCTCCGACGCCTTCACCCCCCTGGTGACCCCAGCCGACCA
TCCGGCCTTCATCTTCTATCCCGAAGCCGCCTGCGGGAGGCCGGCCGGGGTGATTCACGCCCATCGCGCCATGGCCGGCG
GCCTGCCGGCGGTGGAGATGTCGCTGACCTTCTTCCCCCAGTTCGGCGACATCGTCTGGACCTCGAGCGAGTGGATGGGA
GCCGAGGCGCTGTTCCGCGCCGTGCTGCCCGCCTGGCATCACGGCGTGCCGGTGGTGGCCTGCCCCGGCCCCTTCGATCC
CGTCCAGGCCCTGGACCTCATGTCGCGCCACGGCGTGCGCACCGCTTATGTGCCTCCCCACCATCTGGGCGCCCTGACCG
AGGCGGCAGCCAGCCGGCCCCACGCCATGCCGCGCGCCATCGCCAGCGGCCCCGAGCCGCTGGACGAGGACCTGCACGAG
CGGGTGATCAAGGTGTTCGGCGTCCATGCCAACGAGGCCTGGGGCGTGCTGGAAAGCGGCGCCGTGGCGGCCAATCTGGC
CGGGCTGATGGAACTGCGCCCGCCCTCGCCCGGCCGGGCGGCGCCGGGGCTGTCCATCGACGCGGTGGACGAGCGCGGCC
GCCCCTTGCGGGCCGGCGATCGCGGCCTGCTGGCGGTGTCGCCCAACGCGCCGGGCAGCTTTCTCGGCTATTGGGGCGAC
GGCGCCGGCGCGGCGCGGCGGGTCAATGGCTGGCTGATCACCGGACGGTCGGGCTTTCGCGATCTCGACCACTATCTGTG
GCCCGATCCGCCATCCCTGCCCGAGGGCGCCGTCCTGGTCGGAGGGTGCCCCGTGCAGCCGGAAGAGGTGGAACTGGCCC
TGGCCGCCCATCCCGACGTGGTCGCGGCGGCGGTACTGGAATGGACGTCGGGCGAGTTGAAGGCCTTCGTGGTGGCGGGG
GGGCGGGCAGCCGATGCCGCCTTCACACGGGAGCTTCAGGCCTGGGTCCGCCTGACCCGCTCGCCGGTGGAGGTTCCGTC
GCGGATGGAGTTCGTCGACGAATTACCCCTGTCCATGGATGGCAGCGTGCGCCGGGCCGACCTGCGCGACCGGCCCATCC
GCGTGGACGCGCCGTCGCCCGACGAGCGTTGGCCGGGCAAGCTGCGCTGA

Upstream 100 bases:

>100_bases
CGGAATCATGGACCTGGGATTCGATGGCGTCATGTTCGACGACGTCGGAACCTATCAGTGGTTCGAGGAACTGATGCCGC
TGGGTGGCTGAGGACGATTC

Downstream 100 bases:

>100_bases
CCGCTGGCGCTTCGGTCTTTGTCCTGTCATGATTCCCCCATTGAGGGAGGGGGATTCCATGGCACGTGCGGCCGACGATA
TCCAGTTGCCGGAAGAGGCG

Product: Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase

Products: AMP; diphosphate; acetyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLTACRSYEEACRTFRWKIPERYNLAFDVCDRQTMAGADGHRTALIVEAADGGVERHTFHMLRLLSNRLANGLAAMGIAP
GDRVAMALPAGLEAAVTLLAVTKMGAVLVPVPLSLGSEPLGWRLADSGARVAVVDPAALPALERARSRLPELERVLAVGG
GGGALDLWSELERSSDAFTPLVTPADHPAFIFYPEAACGRPAGVIHAHRAMAGGLPAVEMSLTFFPQFGDIVWTSSEWMG
AEALFRAVLPAWHHGVPVVACPGPFDPVQALDLMSRHGVRTAYVPPHHLGALTEAAASRPHAMPRAIASGPEPLDEDLHE
RVIKVFGVHANEAWGVLESGAVAANLAGLMELRPPSPGRAAPGLSIDAVDERGRPLRAGDRGLLAVSPNAPGSFLGYWGD
GAGAARRVNGWLITGRSGFRDLDHYLWPDPPSLPEGAVLVGGCPVQPEEVELALAAHPDVVAAAVLEWTSGELKAFVVAG
GRAADAAFTRELQAWVRLTRSPVEVPSRMEFVDELPLSMDGSVRRADLRDRPIRVDAPSPDERWPGKLR

Sequences:

>Translated_549_residues
MLTACRSYEEACRTFRWKIPERYNLAFDVCDRQTMAGADGHRTALIVEAADGGVERHTFHMLRLLSNRLANGLAAMGIAP
GDRVAMALPAGLEAAVTLLAVTKMGAVLVPVPLSLGSEPLGWRLADSGARVAVVDPAALPALERARSRLPELERVLAVGG
GGGALDLWSELERSSDAFTPLVTPADHPAFIFYPEAACGRPAGVIHAHRAMAGGLPAVEMSLTFFPQFGDIVWTSSEWMG
AEALFRAVLPAWHHGVPVVACPGPFDPVQALDLMSRHGVRTAYVPPHHLGALTEAAASRPHAMPRAIASGPEPLDEDLHE
RVIKVFGVHANEAWGVLESGAVAANLAGLMELRPPSPGRAAPGLSIDAVDERGRPLRAGDRGLLAVSPNAPGSFLGYWGD
GAGAARRVNGWLITGRSGFRDLDHYLWPDPPSLPEGAVLVGGCPVQPEEVELALAAHPDVVAAAVLEWTSGELKAFVVAG
GRAADAAFTRELQAWVRLTRSPVEVPSRMEFVDELPLSMDGSVRRADLRDRPIRVDAPSPDERWPGKLR
>Mature_549_residues
MLTACRSYEEACRTFRWKIPERYNLAFDVCDRQTMAGADGHRTALIVEAADGGVERHTFHMLRLLSNRLANGLAAMGIAP
GDRVAMALPAGLEAAVTLLAVTKMGAVLVPVPLSLGSEPLGWRLADSGARVAVVDPAALPALERARSRLPELERVLAVGG
GGGALDLWSELERSSDAFTPLVTPADHPAFIFYPEAACGRPAGVIHAHRAMAGGLPAVEMSLTFFPQFGDIVWTSSEWMG
AEALFRAVLPAWHHGVPVVACPGPFDPVQALDLMSRHGVRTAYVPPHHLGALTEAAASRPHAMPRAIASGPEPLDEDLHE
RVIKVFGVHANEAWGVLESGAVAANLAGLMELRPPSPGRAAPGLSIDAVDERGRPLRAGDRGLLAVSPNAPGSFLGYWGD
GAGAARRVNGWLITGRSGFRDLDHYLWPDPPSLPEGAVLVGGCPVQPEEVELALAAHPDVVAAAVLEWTSGELKAFVVAG
GRAADAAFTRELQAWVRLTRSPVEVPSRMEFVDELPLSMDGSVRRADLRDRPIRVDAPSPDERWPGKLR

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=546, Percent_Identity=27.8388278388278, Blast_Score=186, Evalue=7e-47,
Organism=Homo sapiens, GI38505220, Length=541, Percent_Identity=27.7264325323475, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI122937307, Length=535, Percent_Identity=27.6635514018692, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI157311624, Length=540, Percent_Identity=27.037037037037, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157311622, Length=540, Percent_Identity=27.037037037037, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI58082049, Length=540, Percent_Identity=27.037037037037, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI115511026, Length=554, Percent_Identity=25.6317689530686, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI195947378, Length=428, Percent_Identity=26.1682242990654, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI42544134, Length=378, Percent_Identity=25.1322751322751, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI13375727, Length=585, Percent_Identity=23.0769230769231, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI8923896, Length=577, Percent_Identity=22.3570190641248, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI253970489, Length=590, Percent_Identity=21.864406779661, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI28416953, Length=558, Percent_Identity=23.8351254480287, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790505, Length=561, Percent_Identity=26.3814616755793, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI221142682, Length=533, Percent_Identity=24.577861163227, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788107, Length=565, Percent_Identity=24.6017699115044, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71983001, Length=565, Percent_Identity=23.5398230088496, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=565, Percent_Identity=23.5398230088496, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=551, Percent_Identity=24.6823956442831, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21355225, Length=579, Percent_Identity=22.9706390328152, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24656500, Length=512, Percent_Identity=22.265625, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21356947, Length=419, Percent_Identity=23.8663484486874, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648260, Length=493, Percent_Identity=21.7038539553753, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.1

Molecular weight: Translated: 58497; Mature: 58497

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00616 HIS_ACID_PHOSPHAT_1 ; PS00758 ARGE_DAPE_CPG2_1 ; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTACRSYEEACRTFRWKIPERYNLAFDVCDRQTMAGADGHRTALIVEAADGGVERHTFH
CCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHCCCCCCCEEEEEECCCCCCHHHHHH
MLRLLSNRLANGLAAMGIAPGDRVAMALPAGLEAAVTLLAVTKMGAVLVPVPLSLGSEPL
HHHHHHHHHHCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCC
GWRLADSGARVAVVDPAALPALERARSRLPELERVLAVGGGGGALDLWSELERSSDAFTP
CEEEECCCCEEEEECCHHHHHHHHHHHHCCHHHHHEEECCCCCHHHHHHHHHHCCCCCCC
LVTPADHPAFIFYPEAACGRPAGVIHAHRAMAGGLPAVEMSLTFFPQFGDIVWTSSEWMG
EECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCC
AEALFRAVLPAWHHGVPVVACPGPFDPVQALDLMSRHGVRTAYVPPHHLGALTEAAASRP
HHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCC
HAMPRAIASGPEPLDEDLHERVIKVFGVHANEAWGVLESGAVAANLAGLMELRPPSPGRA
CCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHEECCCCCCCC
APGLSIDAVDERGRPLRAGDRGLLAVSPNAPGSFLGYWGDGAGAARRVNGWLITGRSGFR
CCCCEEECHHCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCEEECCEEEECCCCCH
DLDHYLWPDPPSLPEGAVLVGGCPVQPEEVELALAAHPDVVAAAVLEWTSGELKAFVVAG
HHHHCCCCCCCCCCCCCEEECCCCCCCHHEEEEEECCCHHHHHHHHHCCCCCEEEEEEEC
GRAADAAFTRELQAWVRLTRSPVEVPSRMEFVDELPLSMDGSVRRADLRDRPIRVDAPSP
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCEEECCCCC
DERWPGKLR
CCCCCCCCC
>Mature Secondary Structure
MLTACRSYEEACRTFRWKIPERYNLAFDVCDRQTMAGADGHRTALIVEAADGGVERHTFH
CCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHCCCCCCCEEEEEECCCCCCHHHHHH
MLRLLSNRLANGLAAMGIAPGDRVAMALPAGLEAAVTLLAVTKMGAVLVPVPLSLGSEPL
HHHHHHHHHHCCHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCC
GWRLADSGARVAVVDPAALPALERARSRLPELERVLAVGGGGGALDLWSELERSSDAFTP
CEEEECCCCEEEEECCHHHHHHHHHHHHCCHHHHHEEECCCCCHHHHHHHHHHCCCCCCC
LVTPADHPAFIFYPEAACGRPAGVIHAHRAMAGGLPAVEMSLTFFPQFGDIVWTSSEWMG
EECCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCCCCC
AEALFRAVLPAWHHGVPVVACPGPFDPVQALDLMSRHGVRTAYVPPHHLGALTEAAASRP
HHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCC
HAMPRAIASGPEPLDEDLHERVIKVFGVHANEAWGVLESGAVAANLAGLMELRPPSPGRA
CCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHEECCCCCCCC
APGLSIDAVDERGRPLRAGDRGLLAVSPNAPGSFLGYWGDGAGAARRVNGWLITGRSGFR
CCCCEEECHHCCCCCCCCCCCEEEEECCCCCCCCEEECCCCCCCCEEECCEEEECCCCCH
DLDHYLWPDPPSLPEGAVLVGGCPVQPEEVELALAAHPDVVAAAVLEWTSGELKAFVVAG
HHHHCCCCCCCCCCCCCEEECCCCCCCHHEEEEEECCCHHHHHHHHHCCCCCEEEEEEEC
GRAADAAFTRELQAWVRLTRSPVEVPSRMEFVDELPLSMDGSVRRADLRDRPIRVDAPSP
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCEEECCCCC
DERWPGKLR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; acetate; CoA

Specific reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

General reaction: Acid-thiol ligation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]