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The map label for this gene is fumC

Identifier: 83312749

GI number: 83312749

Start: 4011898

End: 4013280

Strand: Reverse

Name: fumC

Synonym: amb3650

Alternate gene names: 83312749

Gene position: 4013280-4011898 (Counterclockwise)

Preceding gene: 83312751

Following gene: 83312748

Centisome position: 80.8

GC content: 64.21

Gene sequence:

>1383_bases
ATGAACGCCACTCGGCCGGAAAAGGATTCCTTCGGCATCATCCAGGTGCCCGAACCTCATATATGGGGAGCCCAGACCCA
ACGGTCGCTGGAATATTTCGCCATATCCGGCGAACGCATGCCGATGGAACTGATATCCGCCCTGGCGCGGGTGAAGGGAG
GCTGCGCCCGGGCCAATGCCGAACTCGGCCTGCTGGCCGCCGGCAAGGCCGATGCCATCATCGCTGCGGCGCAGGAGGTG
CTGTCCGGCCGACATGGAGAGGAATTTCCCCTGTCGGTCTGGCAGACCGGCTCGGGGACCCAGACCAACATGAACATGAA
CGAGGTTCTGGCCAACCGCGCCTCCGAAATCCTGGGGGGTGAGCGCGGACTGAACCGCTGTGTCCACGCCAATGACGACG
TCAATCTGGGGCAATCCTCCAACGACGTCTTTCCCACCGCCATGCATGTGGCGGCAGCCGTCACGGTGGTGACGGGCCTG
CTGCCGTCACTCGCCCACCTGCGAAAGACCCTGGTGGGGCTGACGGAAGAGTTCGCTGCTATCGTCAAGATCGGCCGCAC
CCATCTTCAGGACGCCACGCCGCTCACCTTAGGACAGGAATTCTCCGGCTACGCGGCGCAGTTGGATCATGCCGAGGCGG
TGCTGCTCGCCACACTGCCGTCCTTGTATCCCCTGGCCATCGGCGGCACCGCAGTGGGGACCGGCCTGAATACCCACCCG
GAATTCGGCGCCCGTGTGGCGGCGCAATTGGCTGCGGATCTTGGCCTGCCGTTCGTTTCCGCCACCAATAAATTCGCCGC
CCTGGCGGCCCATGACGGGATGGTTGCCATGCACGGCGCCGTCAAGACCCTGGCGGCTGCACTGATGAAGATCGCCAACG
ACATCCGCTGGCTGGCTTCGGGGCCGCGCTGCGGATTGGGCGAGATCAGCCTGCCGGAAAACGAACCGGGCAGTTCGATC
ATGCCGGGCAAGGTCAATCCAACCCAGTGCGAGGCTCTGACCATGGCCTGTTGCCAAGTGATGGGCAACGATGTGGCCAT
CACAATGGGCGGCGCCTCGGGAAATTTCGAGCTGAACGTCTTCAAACCGCTGATCGCCCACAATTTCCTTCAGAGCGTCC
GCCTCATGGCCGACGGCATGGCGAGCTTCGAACGCCATTGCGTGCGCGGCATCACCGCCAACCGGCAGCGCATCGATCAA
TTGCTGGAGCAGTCGCTGATGCTGGTGACGGCGTTGGCGCCCCATATCGGCTATGACCGCGCCGCCGAGATCGCCAAGTC
CGCCCATGCCGGGGGGGCGACCCTGCGCCAAGCCGCCCTGGCGACGGGCTACGTGACGGCGGAGCAATTCGACGTCTGGG
TGGACCCGACGATCATGGTCTGA

Upstream 100 bases:

>100_bases
GGGAAAGCCTTTCATGGCCGAGCGAAAGATCGCGCAACGCGCCGAACGGATCTGGGAGCATGATGATCTCCAACGAGGTC
AGCCTGGAGAGGAGCCGCGC

Downstream 100 bases:

>100_bases
CGCGGCTCGCAGCCTGCCGTCAACGTCATCAGGATTTCGGTATACCGCCCCCCTAATTTGCCGTGATACCGACCGAGTTG
GGCTTGGCTCAGGTCATGCG

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNATRPEKDSFGIIQVPEPHIWGAQTQRSLEYFAISGERMPMELISALARVKGGCARANAELGLLAAGKADAIIAAAQEV
LSGRHGEEFPLSVWQTGSGTQTNMNMNEVLANRASEILGGERGLNRCVHANDDVNLGQSSNDVFPTAMHVAAAVTVVTGL
LPSLAHLRKTLVGLTEEFAAIVKIGRTHLQDATPLTLGQEFSGYAAQLDHAEAVLLATLPSLYPLAIGGTAVGTGLNTHP
EFGARVAAQLAADLGLPFVSATNKFAALAAHDGMVAMHGAVKTLAAALMKIANDIRWLASGPRCGLGEISLPENEPGSSI
MPGKVNPTQCEALTMACCQVMGNDVAITMGGASGNFELNVFKPLIAHNFLQSVRLMADGMASFERHCVRGITANRQRIDQ
LLEQSLMLVTALAPHIGYDRAAEIAKSAHAGGATLRQAALATGYVTAEQFDVWVDPTIMV

Sequences:

>Translated_460_residues
MNATRPEKDSFGIIQVPEPHIWGAQTQRSLEYFAISGERMPMELISALARVKGGCARANAELGLLAAGKADAIIAAAQEV
LSGRHGEEFPLSVWQTGSGTQTNMNMNEVLANRASEILGGERGLNRCVHANDDVNLGQSSNDVFPTAMHVAAAVTVVTGL
LPSLAHLRKTLVGLTEEFAAIVKIGRTHLQDATPLTLGQEFSGYAAQLDHAEAVLLATLPSLYPLAIGGTAVGTGLNTHP
EFGARVAAQLAADLGLPFVSATNKFAALAAHDGMVAMHGAVKTLAAALMKIANDIRWLASGPRCGLGEISLPENEPGSSI
MPGKVNPTQCEALTMACCQVMGNDVAITMGGASGNFELNVFKPLIAHNFLQSVRLMADGMASFERHCVRGITANRQRIDQ
LLEQSLMLVTALAPHIGYDRAAEIAKSAHAGGATLRQAALATGYVTAEQFDVWVDPTIMV
>Mature_460_residues
MNATRPEKDSFGIIQVPEPHIWGAQTQRSLEYFAISGERMPMELISALARVKGGCARANAELGLLAAGKADAIIAAAQEV
LSGRHGEEFPLSVWQTGSGTQTNMNMNEVLANRASEILGGERGLNRCVHANDDVNLGQSSNDVFPTAMHVAAAVTVVTGL
LPSLAHLRKTLVGLTEEFAAIVKIGRTHLQDATPLTLGQEFSGYAAQLDHAEAVLLATLPSLYPLAIGGTAVGTGLNTHP
EFGARVAAQLAADLGLPFVSATNKFAALAAHDGMVAMHGAVKTLAAALMKIANDIRWLASGPRCGLGEISLPENEPGSSI
MPGKVNPTQCEALTMACCQVMGNDVAITMGGASGNFELNVFKPLIAHNFLQSVRLMADGMASFERHCVRGITANRQRIDQ
LLEQSLMLVTALAPHIGYDRAAEIAKSAHAGGATLRQAALATGYVTAEQFDVWVDPTIMV

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=59.4360086767896, Blast_Score=532, Evalue=1e-151,
Organism=Homo sapiens, GI266458395, Length=63, Percent_Identity=55.5555555555556, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1787896, Length=460, Percent_Identity=67.8260869565217, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI87082375, Length=453, Percent_Identity=38.1898454746137, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=59.95670995671, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32565146, Length=325, Percent_Identity=63.0769230769231, Blast_Score=423, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6324993, Length=463, Percent_Identity=56.1555075593952, Blast_Score=511, Evalue=1e-145,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=57.0498915401301, Blast_Score=538, Evalue=1e-153,
Organism=Drosophila melanogaster, GI24640179, Length=459, Percent_Identity=57.0806100217865, Blast_Score=535, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24640177, Length=459, Percent_Identity=57.0806100217865, Blast_Score=535, Evalue=1e-152,
Organism=Drosophila melanogaster, GI24662684, Length=461, Percent_Identity=55.3145336225597, Blast_Score=506, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24583245, Length=461, Percent_Identity=50.9761388286334, Blast_Score=459, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 48429; Mature: 48429

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATRPEKDSFGIIQVPEPHIWGAQTQRSLEYFAISGERMPMELISALARVKGGCARANA
CCCCCCCCCCCCEEECCCCCCCCCHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
ELGLLAAGKADAIIAAAQEVLSGRHGEEFPLSVWQTGSGTQTNMNMNEVLANRASEILGG
CEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCC
ERGLNRCVHANDDVNLGQSSNDVFPTAMHVAAAVTVVTGLLPSLAHLRKTLVGLTEEFAA
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVKIGRTHLQDATPLTLGQEFSGYAAQLDHAEAVLLATLPSLYPLAIGGTAVGTGLNTHP
HHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHCCHHHEEECCHHHCCCCCCCC
EFGARVAAQLAADLGLPFVSATNKFAALAAHDGMVAMHGAVKTLAAALMKIANDIRWLAS
HHHHHHHHHHHHHHCCCCHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GPRCGLGEISLPENEPGSSIMPGKVNPTQCEALTMACCQVMGNDVAITMGGASGNFELNV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEH
FKPLIAHNFLQSVRLMADGMASFERHCVRGITANRQRIDQLLEQSLMLVTALAPHIGYDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
AAEIAKSAHAGGATLRQAALATGYVTAEQFDVWVDPTIMV
HHHHHHHHCCCCHHHHHHHHHHCCEEHHHEEEEECCEEEC
>Mature Secondary Structure
MNATRPEKDSFGIIQVPEPHIWGAQTQRSLEYFAISGERMPMELISALARVKGGCARANA
CCCCCCCCCCCCEEECCCCCCCCCHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCC
ELGLLAAGKADAIIAAAQEVLSGRHGEEFPLSVWQTGSGTQTNMNMNEVLANRASEILGG
CEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCC
ERGLNRCVHANDDVNLGQSSNDVFPTAMHVAAAVTVVTGLLPSLAHLRKTLVGLTEEFAA
HHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVKIGRTHLQDATPLTLGQEFSGYAAQLDHAEAVLLATLPSLYPLAIGGTAVGTGLNTHP
HHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHHHHHHHHCCHHHEEECCHHHCCCCCCCC
EFGARVAAQLAADLGLPFVSATNKFAALAAHDGMVAMHGAVKTLAAALMKIANDIRWLAS
HHHHHHHHHHHHHHCCCCHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GPRCGLGEISLPENEPGSSIMPGKVNPTQCEALTMACCQVMGNDVAITMGGASGNFELNV
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEH
FKPLIAHNFLQSVRLMADGMASFERHCVRGITANRQRIDQLLEQSLMLVTALAPHIGYDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHH
AAEIAKSAHAGGATLRQAALATGYVTAEQFDVWVDPTIMV
HHHHHHHHCCCCHHHHHHHHHHCCEEHHHEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]