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The map label for this gene is pyrB [H]

Identifier: 83312714

GI number: 83312714

Start: 3968468

End: 3969193

Strand: Reverse

Name: pyrB [H]

Synonym: amb3615

Alternate gene names: 83312714

Gene position: 3969193-3968468 (Counterclockwise)

Preceding gene: 83312719

Following gene: 83312713

Centisome position: 79.91

GC content: 65.7

Gene sequence:

>726_bases
GTGATCAACATGCAGTCGGCGGGCTCGTCGGTGCAGAAGGGCGAGACCCTGATCGACACCGCCATGACGCTCAACGCCAT
GCATCTGGACGTGCTGGTGGTGCGCCACCCGGATTCGGGCGCGGTCAAGCTGCTGAGCGAAAAGGTTAATTGCGCCGTGA
TCAACGGCGGCGACGGCAGCCACGAGCACCCCACCCAGGCCTTGCTCGACGCCCTGACCATCCGGCGCCGCAAGGGCAAA
CTGTCGGGCCTCAACGTCGCCATCTGCGGCGACGTGCGCCATTCCCGCGTGGCCCGCTCCAACATCTACCTGCTCAACGC
CATGGGCGCCCATGTGCGCCTGATCGGCCCGCGCACCCTGTTGCCCTCGGGCCTCGACCGCATGGGCGTCGAGGTCTTCC
ACGACATGCGCAAGGGTCTGGCCGACGTGGACGTCATCATGATGCTGCGCATCCAGAACGAGCGCATGAGCGGCAATTTC
ATCCCCAGCATCCGCGAGTACTTCCACTTCTTCGGCCTGGACCGCGAGAAGCTGGGCATCGCCAAGCCCGATGCGGTGAT
CATGCATCCCGGTCCCATGAACCGCGGCGTCGAGATCGATTCCGACGTGGCCGACGACGTGGAACGCTCGCTGATCCGTT
CGCAGGTGGAGATGGGCGTGGCGGTGCGCATGGCCTGCCTGGACATGCTGACCCGCGACCTGACCCGCTCGGAAGGAGCC
GTCTGA

Upstream 100 bases:

>100_bases
GAATCTCCTGCGCGGCCGCACGGTCATCAACCTGTTCTACGAGAACTCGACGCGCACGCGCACCAGCTTCGAGCTGGCGG
GCAAGCGTCTGGGCGCCGAT

Downstream 100 bases:

>100_bases
TGGCCGCCCGCAATTCCTCGGGCCTGGTGGCCTATGTCAACGCCCGCCTGCTGGACCCGGCCACCGGGCTGGATACCCGG
GGCGCCCTGCTGACCAATGG

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MINMQSAGSSVQKGETLIDTAMTLNAMHLDVLVVRHPDSGAVKLLSEKVNCAVINGGDGSHEHPTQALLDALTIRRRKGK
LSGLNVAICGDVRHSRVARSNIYLLNAMGAHVRLIGPRTLLPSGLDRMGVEVFHDMRKGLADVDVIMMLRIQNERMSGNF
IPSIREYFHFFGLDREKLGIAKPDAVIMHPGPMNRGVEIDSDVADDVERSLIRSQVEMGVAVRMACLDMLTRDLTRSEGA
V

Sequences:

>Translated_241_residues
MINMQSAGSSVQKGETLIDTAMTLNAMHLDVLVVRHPDSGAVKLLSEKVNCAVINGGDGSHEHPTQALLDALTIRRRKGK
LSGLNVAICGDVRHSRVARSNIYLLNAMGAHVRLIGPRTLLPSGLDRMGVEVFHDMRKGLADVDVIMMLRIQNERMSGNF
IPSIREYFHFFGLDREKLGIAKPDAVIMHPGPMNRGVEIDSDVADDVERSLIRSQVEMGVAVRMACLDMLTRDLTRSEGA
V
>Mature_241_residues
MINMQSAGSSVQKGETLIDTAMTLNAMHLDVLVVRHPDSGAVKLLSEKVNCAVINGGDGSHEHPTQALLDALTIRRRKGK
LSGLNVAICGDVRHSRVARSNIYLLNAMGAHVRLIGPRTLLPSGLDRMGVEVFHDMRKGLADVDVIMMLRIQNERMSGNF
IPSIREYFHFFGLDREKLGIAKPDAVIMHPGPMNRGVEIDSDVADDVERSLIRSQVEMGVAVRMACLDMLTRDLTRSEGA
V

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=239, Percent_Identity=38.4937238493724, Blast_Score=137, Evalue=9e-33,
Organism=Escherichia coli, GI2367364, Length=240, Percent_Identity=35, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI193204318, Length=247, Percent_Identity=33.1983805668016, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322331, Length=241, Percent_Identity=36.5145228215768, Blast_Score=123, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24642586, Length=240, Percent_Identity=35, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 26434; Mature: 26434

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINMQSAGSSVQKGETLIDTAMTLNAMHLDVLVVRHPDSGAVKLLSEKVNCAVINGGDGS
CCCCCCCCCCHHCCHHHHHHHHHHCEEEEEEEEEECCCCCHHHHHHCCCCEEEEECCCCC
HEHPTQALLDALTIRRRKGKLSGLNVAICGDVRHSRVARSNIYLLNAMGAHVRLIGPRTL
CCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCEEEEEECCCEEEEECCHHH
LPSGLDRMGVEVFHDMRKGLADVDVIMMLRIQNERMSGNFIPSIREYFHFFGLDREKLGI
CCCCHHHHHHHHHHHHHHCHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHCCC
AKPDAVIMHPGPMNRGVEIDSDVADDVERSLIRSQVEMGVAVRMACLDMLTRDLTRSEGA
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
V
C
>Mature Secondary Structure
MINMQSAGSSVQKGETLIDTAMTLNAMHLDVLVVRHPDSGAVKLLSEKVNCAVINGGDGS
CCCCCCCCCCHHCCHHHHHHHHHHCEEEEEEEEEECCCCCHHHHHHCCCCEEEEECCCCC
HEHPTQALLDALTIRRRKGKLSGLNVAICGDVRHSRVARSNIYLLNAMGAHVRLIGPRTL
CCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCEEEEEECCCEEEEECCHHH
LPSGLDRMGVEVFHDMRKGLADVDVIMMLRIQNERMSGNFIPSIREYFHFFGLDREKLGI
CCCCHHHHHHHHHHHHHHCHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHCCC
AKPDAVIMHPGPMNRGVEIDSDVADDVERSLIRSQVEMGVAVRMACLDMLTRDLTRSEGA
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA