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The map label for this gene is ydfH [H]

Identifier: 83312684

GI number: 83312684

Start: 3936810

End: 3937469

Strand: Reverse

Name: ydfH [H]

Synonym: amb3585

Alternate gene names: 83312684

Gene position: 3937469-3936810 (Counterclockwise)

Preceding gene: 83312695

Following gene: 83312683

Centisome position: 79.27

GC content: 65.15

Gene sequence:

>660_bases
ATGAACGAGCAAAAAAAGCCGGCGACCCGGGCCGACGAGATCCGTCAGGTGATCGAACGGGAGATCTTCCAGGGTCTTTT
GCGCCCCGGCACCCGCCTGGACGAGGAGAGCCTGGCCACCCGCTTCGGCCTGTCGCGCACTCCGGTGCGCGAAGCCATCC
TGCAACTGGTCTATTCCGGCCTGGTGGTGAAGAAGCCGCGTCAAGGCGCCGTGGTGGCGCCCCTGGATCTGCACCGCATG
GTGCAGATGTTCGAGGTGATGAGCGACATCGAGGGGCTGTGCGCCCGCTATGCCGCGCGCCGCATGTCGCCGGAGGAAAA
GTCCAGGCTTTCCGCCATGGTGGCCGAGGCCGGAACCCTGTGCGAGGCCCGCGACCTGGACGGCTATTACGCCCTGAACC
GCGCTTTCCACGACGCGGTTTACGACGGCAGCCATAACGAGGTGTTGAAGGAGATGGCGCGGTCCCTCTATGCCCGCCTC
GCCCCCTATCGCCGTTATCAGCTCAACCATCCCGGCCGCATCGACGGCTCGCTGGCCGAGCATGCCGAGGTGGTGGCGGC
CATCGAATCGGCCGATCCCGACCGCGCCCAGGCGGTGATGCGCAAGCATGTCACCATCCAGGCCGACATCTTTTCGGAAT
TCGTGTCCATCATGAGCTAG

Upstream 100 bases:

>100_bases
CGTGATACACAGATTTTGTGTCCGAAGAGCGGATTGCCCTTAGACAGTGTCGGCGGGAGATGTATACTCGGCACCGCCAC
ATCAGGGGTTTGGAGGCCCG

Downstream 100 bases:

>100_bases
TGTTGTATCCGAGAAAGGCATTTCTTGGATACAAAATGGTGGCGCGGGGCATGCCCATATGGTATCGCCGTAATCAGGCT
TTGATGACAAAGGGGCGCGC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNEQKKPATRADEIRQVIEREIFQGLLRPGTRLDEESLATRFGLSRTPVREAILQLVYSGLVVKKPRQGAVVAPLDLHRM
VQMFEVMSDIEGLCARYAARRMSPEEKSRLSAMVAEAGTLCEARDLDGYYALNRAFHDAVYDGSHNEVLKEMARSLYARL
APYRRYQLNHPGRIDGSLAEHAEVVAAIESADPDRAQAVMRKHVTIQADIFSEFVSIMS

Sequences:

>Translated_219_residues
MNEQKKPATRADEIRQVIEREIFQGLLRPGTRLDEESLATRFGLSRTPVREAILQLVYSGLVVKKPRQGAVVAPLDLHRM
VQMFEVMSDIEGLCARYAARRMSPEEKSRLSAMVAEAGTLCEARDLDGYYALNRAFHDAVYDGSHNEVLKEMARSLYARL
APYRRYQLNHPGRIDGSLAEHAEVVAAIESADPDRAQAVMRKHVTIQADIFSEFVSIMS
>Mature_219_residues
MNEQKKPATRADEIRQVIEREIFQGLLRPGTRLDEESLATRFGLSRTPVREAILQLVYSGLVVKKPRQGAVVAPLDLHRM
VQMFEVMSDIEGLCARYAARRMSPEEKSRLSAMVAEAGTLCEARDLDGYYALNRAFHDAVYDGSHNEVLKEMARSLYARL
APYRRYQLNHPGRIDGSLAEHAEVVAAIESADPDRAQAVMRKHVTIQADIFSEFVSIMS

Specific function: Unknown

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787821, Length=192, Percent_Identity=25, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI87081914, Length=196, Percent_Identity=24.4897959183673, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI48994961, Length=208, Percent_Identity=30.2884615384615, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082149, Length=195, Percent_Identity=26.1538461538462, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1786303, Length=225, Percent_Identity=28, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24677; Mature: 24677

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQKKPATRADEIRQVIEREIFQGLLRPGTRLDEESLATRFGLSRTPVREAILQLVYSG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHC
LVVKKPRQGAVVAPLDLHRMVQMFEVMSDIEGLCARYAARRMSPEEKSRLSAMVAEAGTL
HHEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
CEARDLDGYYALNRAFHDAVYDGSHNEVLKEMARSLYARLAPYRRYQLNHPGRIDGSLAE
HHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
HAEVVAAIESADPDRAQAVMRKHVTIQADIFSEFVSIMS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNEQKKPATRADEIRQVIEREIFQGLLRPGTRLDEESLATRFGLSRTPVREAILQLVYSG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHC
LVVKKPRQGAVVAPLDLHRMVQMFEVMSDIEGLCARYAARRMSPEEKSRLSAMVAEAGTL
HHEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
CEARDLDGYYALNRAFHDAVYDGSHNEVLKEMARSLYARLAPYRRYQLNHPGRIDGSLAE
HHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
HAEVVAAIESADPDRAQAVMRKHVTIQADIFSEFVSIMS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]