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The map label for this gene is cysW [H]

Identifier: 83312664

GI number: 83312664

Start: 3919793

End: 3920665

Strand: Reverse

Name: cysW [H]

Synonym: amb3565

Alternate gene names: 83312664

Gene position: 3920665-3919793 (Counterclockwise)

Preceding gene: 83312665

Following gene: 83312662

Centisome position: 78.93

GC content: 68.61

Gene sequence:

>873_bases
ATGATTCCCGCCATGGCCGTTCACCACGTTCCCCGCCGTCGTCCCGGCGGTGCCCGGCGCGCCCTGCTGATCGGGCTGGC
CGGGCTGCTGGCCCTGGGCTTCCTGATGGTGCCCATGGCGGTGATCTTCGCCCAGGCCCTGTCCAAGGGCTGGGCGGCGT
GGTGGGCGGCGGTCAGCCATCCCGAGACCCTGCACGCCATCGGCCTGTCCCTGGTGGTGGTGGCGGTGGTGGTGCCCGTC
AACGCCGTCTACGGGCTGGCGGTGGCCTGGGCAGTGACCAAGTTCCGCTTCAAGGGCAAGAAATGGCTGGTGGCCCTGGT
GGAGGCGCCGTTCTCGGTGTCGCCCATCGTCGCCGGCGTCGCCGCCCTGATGGTCTATGGCGGCTCCGGCCTGCTGGGAC
CCTGGCTGGAGGAGCACGACATCAAGGTGATGTTCGCGCTGCCGGGCATCGTCATCGCCACCCTGTTCGTCACCAGCCCG
TTCATCGCCCGCGAGCTTTTGCCGCTGATGCAGCTGCAGGGCACCGATGACGAGGAAGCCGCCCTGACCCTGGGGGCGGG
GGGGCTGTCCATCTTCCTGCGGGTGACGCTGCCCAACGTCAAATGGGCGCTGTTTTACGGCATCATCCTTTGCGCCGCCC
GTTCGCTGGGCGAATTCGGCGCCGTCTCGGTGGTGTCGGGCCAGGTGCGGGGCGAGACCATGACCCTGCCGCTGCAGATC
GATCTGCTGTATCACGACTATGACAGTTCGGGGGCCTTCGCCGCGGCGTCGGTGCTGACCCTGCTGGCCCTGGTGACCCT
GGGGCTGAAGCTGCTGGTCGAGCGCTTCTACGACCGCGAGATCGAGGACACCCTGGCCGAGCGCCAGGGATGA

Upstream 100 bases:

>100_bases
GCCATCGCCACCGTGGTGCTGGCCTTCGCCTTCGTCACCATGCTGCTGACCAACGGCATCCAGGCCTGGCAGCAGCGCTA
CCTGGCAAAGGGGTGAGACG

Downstream 100 bases:

>100_bases
TCAGGCCTGGCCCCCGGGCAAGGTGAAGCGGAACCTCGATCCCTCTCCGGGCACTGATTCCACCCAGATTCGGCCGCCGT
GATGCTCGACGATCTTGCGG

Product: ABC-type sulfate transport system, permease component

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MIPAMAVHHVPRRRPGGARRALLIGLAGLLALGFLMVPMAVIFAQALSKGWAAWWAAVSHPETLHAIGLSLVVVAVVVPV
NAVYGLAVAWAVTKFRFKGKKWLVALVEAPFSVSPIVAGVAALMVYGGSGLLGPWLEEHDIKVMFALPGIVIATLFVTSP
FIARELLPLMQLQGTDDEEAALTLGAGGLSIFLRVTLPNVKWALFYGIILCAARSLGEFGAVSVVSGQVRGETMTLPLQI
DLLYHDYDSSGAFAAASVLTLLALVTLGLKLLVERFYDREIEDTLAERQG

Sequences:

>Translated_290_residues
MIPAMAVHHVPRRRPGGARRALLIGLAGLLALGFLMVPMAVIFAQALSKGWAAWWAAVSHPETLHAIGLSLVVVAVVVPV
NAVYGLAVAWAVTKFRFKGKKWLVALVEAPFSVSPIVAGVAALMVYGGSGLLGPWLEEHDIKVMFALPGIVIATLFVTSP
FIARELLPLMQLQGTDDEEAALTLGAGGLSIFLRVTLPNVKWALFYGIILCAARSLGEFGAVSVVSGQVRGETMTLPLQI
DLLYHDYDSSGAFAAASVLTLLALVTLGLKLLVERFYDREIEDTLAERQG
>Mature_290_residues
MIPAMAVHHVPRRRPGGARRALLIGLAGLLALGFLMVPMAVIFAQALSKGWAAWWAAVSHPETLHAIGLSLVVVAVVVPV
NAVYGLAVAWAVTKFRFKGKKWLVALVEAPFSVSPIVAGVAALMVYGGSGLLGPWLEEHDIKVMFALPGIVIATLFVTSP
FIARELLPLMQLQGTDDEEAALTLGAGGLSIFLRVTLPNVKWALFYGIILCAARSLGEFGAVSVVSGQVRGETMTLPLQI
DLLYHDYDSSGAFAAASVLTLLALVTLGLKLLVERFYDREIEDTLAERQG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=268, Percent_Identity=50, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788764, Length=215, Percent_Identity=36.2790697674419, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786980, Length=225, Percent_Identity=30.6666666666667, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30965; Mature: 30965

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPAMAVHHVPRRRPGGARRALLIGLAGLLALGFLMVPMAVIFAQALSKGWAAWWAAVSH
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PETLHAIGLSLVVVAVVVPVNAVYGLAVAWAVTKFRFKGKKWLVALVEAPFSVSPIVAGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCHHHHHHH
AALMVYGGSGLLGPWLEEHDIKVMFALPGIVIATLFVTSPFIARELLPLMQLQGTDDEEA
HHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHH
ALTLGAGGLSIFLRVTLPNVKWALFYGIILCAARSLGEFGAVSVVSGQVRGETMTLPLQI
EEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEE
DLLYHDYDSSGAFAAASVLTLLALVTLGLKLLVERFYDREIEDTLAERQG
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MIPAMAVHHVPRRRPGGARRALLIGLAGLLALGFLMVPMAVIFAQALSKGWAAWWAAVSH
CCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PETLHAIGLSLVVVAVVVPVNAVYGLAVAWAVTKFRFKGKKWLVALVEAPFSVSPIVAGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCHHHHHHH
AALMVYGGSGLLGPWLEEHDIKVMFALPGIVIATLFVTSPFIARELLPLMQLQGTDDEEA
HHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHH
ALTLGAGGLSIFLRVTLPNVKWALFYGIILCAARSLGEFGAVSVVSGQVRGETMTLPLQI
EEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEE
DLLYHDYDSSGAFAAASVLTLLALVTLGLKLLVERFYDREIEDTLAERQG
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]