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The map label for this gene is ybfF [H]

Identifier: 83312540

GI number: 83312540

Start: 3774610

End: 3775380

Strand: Reverse

Name: ybfF [H]

Synonym: amb3441

Alternate gene names: 83312540

Gene position: 3775380-3774610 (Counterclockwise)

Preceding gene: 83312541

Following gene: 83312539

Centisome position: 76.01

GC content: 68.61

Gene sequence:

>771_bases
ATGCGGCTTCACGCCATCACGGCCGGTCACGGCGCTCCCCATGGGGTGCCGCTGCTGATCCTGCACGGCCTGTTGGGCTC
GGCCCGCAATTGGGGGGCGGTGGTCAAGACCCTGGGTGAGACCCGGCGGGTTCTGGCTCTCGACCTGCCCAATCACGGCG
CCAGCCCGTGGACCGAGATCATGGATTACCCTTTCATGGCCCGCGAACTGGCGGCCGTGATCGATCACCTGGGCGGCAGG
GCGGCGGTCATGGGCCATTCCATGGGCGGCAAGGCGGCCATGACCCTGGCCCTGACCCGCCCCGACATGGTGGAGCGGCT
GGTGGTAGTGGACATCGCCCCGGTCTCCTACAGCCATACCTTCGCCCCCTATATCAAGGCCATGCGCGGCGTGCCCCTGG
CCGAGATCTCGTCGCGGGGAGAGGTGGAGGCCGCCCTGGCCGCCGCCATTCCCGACAAGGGCGTGCGCGCCTTCCTGATG
CAGAATCTGGAGGGCGGCGCCGGCGGTTATCGCTGGCGGCCCAATCTGGCGGTGCTGGGCGCCCATATGGACGACATCCT
GGCCTTTCCGCCCTTTCCCGACGGCGCCTGCTATGAGGGGCCGACCCTGTTCGTGGCCGGAGAAACCTCGGATTACATCC
GGCCGGCGCACGAGGACGTGATCGCCCAGTTCTTCCCCCGCGCCGAGACCGTGGAAGTCCCGGGCGCGGGCCATTGGGTC
CATGCCGACAACCCGTCCGGCTTCATGGCGGCGATCGGGTCTTTCCTGTAG

Upstream 100 bases:

>100_bases
GGCGGTGGAGTTCGTCCGTCCCACCTTCTCGCTGATGAAGGTGGTGGGGCGCCATATCGGCCGGCTGCTGGTCGGCCGGT
CCCATCTGTTCAGGCACTAG

Downstream 100 bases:

>100_bases
TCATTGCGTTTTCCGGCCGTGATTTGATAGAAGTCAAATCACGAGGTGGAGGTCGGTTCGAGTGCAGGTCATCGACGCTG
TCAAACTGCAGCGCAATATC

Product: hydrolase or acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRLHAITAGHGAPHGVPLLILHGLLGSARNWGAVVKTLGETRRVLALDLPNHGASPWTEIMDYPFMARELAAVIDHLGGR
AAVMGHSMGGKAAMTLALTRPDMVERLVVVDIAPVSYSHTFAPYIKAMRGVPLAEISSRGEVEAALAAAIPDKGVRAFLM
QNLEGGAGGYRWRPNLAVLGAHMDDILAFPPFPDGACYEGPTLFVAGETSDYIRPAHEDVIAQFFPRAETVEVPGAGHWV
HADNPSGFMAAIGSFL

Sequences:

>Translated_256_residues
MRLHAITAGHGAPHGVPLLILHGLLGSARNWGAVVKTLGETRRVLALDLPNHGASPWTEIMDYPFMARELAAVIDHLGGR
AAVMGHSMGGKAAMTLALTRPDMVERLVVVDIAPVSYSHTFAPYIKAMRGVPLAEISSRGEVEAALAAAIPDKGVRAFLM
QNLEGGAGGYRWRPNLAVLGAHMDDILAFPPFPDGACYEGPTLFVAGETSDYIRPAHEDVIAQFFPRAETVEVPGAGHWV
HADNPSGFMAAIGSFL
>Mature_256_residues
MRLHAITAGHGAPHGVPLLILHGLLGSARNWGAVVKTLGETRRVLALDLPNHGASPWTEIMDYPFMARELAAVIDHLGGR
AAVMGHSMGGKAAMTLALTRPDMVERLVVVDIAPVSYSHTFAPYIKAMRGVPLAEISSRGEVEAALAAAIPDKGVRAFLM
QNLEGGAGGYRWRPNLAVLGAHMDDILAFPPFPDGACYEGPTLFVAGETSDYIRPAHEDVIAQFFPRAETVEVPGAGHWV
HADNPSGFMAAIGSFL

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=249, Percent_Identity=44.578313253012, Blast_Score=197, Evalue=6e-51,
Organism=Homo sapiens, GI23200008, Length=249, Percent_Identity=44.578313253012, Blast_Score=197, Evalue=6e-51,
Organism=Homo sapiens, GI223941839, Length=242, Percent_Identity=35.5371900826446, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1786902, Length=242, Percent_Identity=33.0578512396694, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17508535, Length=255, Percent_Identity=38.4313725490196, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17507063, Length=269, Percent_Identity=30.8550185873606, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321468, Length=273, Percent_Identity=31.8681318681319, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640348, Length=268, Percent_Identity=32.8358208955224, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24646055, Length=255, Percent_Identity=27.0588235294118, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 27230; Mature: 27230

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLHAITAGHGAPHGVPLLILHGLLGSARNWGAVVKTLGETRRVLALDLPNHGASPWTEI
CEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
MDYPFMARELAAVIDHLGGRAAVMGHSMGGKAAMTLALTRPDMVERLVVVDIAPVSYSHT
HCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCHHHHHEEEEEEECCCCCCCH
FAPYIKAMRGVPLAEISSRGEVEAALAAAIPDKGVRAFLMQNLEGGAGGYRWRPNLAVLG
HHHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEECCCEEEEE
AHMDDILAFPPFPDGACYEGPTLFVAGETSDYIRPAHEDVIAQFFPRAETVEVPGAGHWV
CCHHHHEECCCCCCCCCCCCCEEEEECCCCHHCCCHHHHHHHHHCCCCCEEECCCCCCEE
HADNPSGFMAAIGSFL
ECCCCCHHHHHHHHCC
>Mature Secondary Structure
MRLHAITAGHGAPHGVPLLILHGLLGSARNWGAVVKTLGETRRVLALDLPNHGASPWTEI
CEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
MDYPFMARELAAVIDHLGGRAAVMGHSMGGKAAMTLALTRPDMVERLVVVDIAPVSYSHT
HCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCHHHHHEEEEEEECCCCCCCH
FAPYIKAMRGVPLAEISSRGEVEAALAAAIPDKGVRAFLMQNLEGGAGGYRWRPNLAVLG
HHHHHHHHCCCCHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEECCCEEEEE
AHMDDILAFPPFPDGACYEGPTLFVAGETSDYIRPAHEDVIAQFFPRAETVEVPGAGHWV
CCHHHHEECCCCCCCCCCCCCEEEEECCCCHHCCCHHHHHHHHHCCCCCEEECCCCCCEE
HADNPSGFMAAIGSFL
ECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]