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The map label for this gene is rne [H]

Identifier: 83312436

GI number: 83312436

Start: 3629272

End: 3630306

Strand: Reverse

Name: rne [H]

Synonym: amb3337

Alternate gene names: 83312436

Gene position: 3630306-3629272 (Counterclockwise)

Preceding gene: 83312437

Following gene: 83312435

Centisome position: 73.09

GC content: 74.11

Gene sequence:

>1035_bases
GTGAGCGGGCGAGTCGAGATCCTCATCTCCGCCGGCCCGGGCGAGGCCCGTTATCTGGTGCTGGCCGACGGCCAGCCGCT
GGAGCTGGTTATCGACCGTCCCTCCCTCCTGCTCGACAGCGTGTTCCGTGGCCGGGTCACCGCCCTGGACAAGGGGCTGG
ACGCCGCCTTCGTGGATCTGGGGACGGGGCCGCGCCCCGGCTTCCTGCCCGGGGCCAGGGCGCTGGGCCTGTCCGAGGGT
TCGGCCGTGGTGGCGAGGGTGCGGGCCGAAGGCCGGGGCGGCAAGGGGCCGCTGCTGACCCCCGCCGGGGATTTCCCGGC
AACGGGCGAGGCTCCGGCCCTATTGCGCCGTCCCGACCCGCTGGACCGTCTGCGCGCCGCCTTTTCCGAGGCCGAAATCG
TCACTGATGCCCACCACGAGGTGGACGAGGTGCTGCAGGCGGTTCTCGACCCCGTGGTGCCGCTGAAAGGCGGCGGGCGC
CTGGTCATCGAGCCGGCCGCCGCCCTGACCGCCATCGACGTGGATTCGGCCGGCGCCCGCCCGGCCGAGGCCAATGGGGC
GGCGGTGACGGAAATCGCCCGCCAGCTTCGCCTCAGGGGCATCGGCGGACAGGTGGTGGTGGATTTCGTCTCGGGCCGCG
ACCGCAAGCCGTTGTTCCGGCTGGCCGAGGCGCTGAAGCAGGCGGTGGCGGCCGACTCGACGCCCACCCATGTATTCGGG
GTGTCGCCCCTGGGGCTGGTGGAACTGACCCGCGAGCGTCGTGGACCCGCCCTGGCCGAGCTGCTGTGCCGCCGCACCCA
GGCCGCCACGCCCGAGACCCTGGCCCTGGCGGCCTTGCGCGCCCTGCTGGCCGAGAGCCTCACCCGGCCGGGCAAGATTC
TGGCCCTGCGTGCCGCCCCCCCGGTGGCCGCCGCCCTAGCCGCCCTGGCCGCCGAGCGTGCGGAAGCCGAGCGCCGCCTG
GGCCACCCCCTGACCATCCGCGAAGACGCCTCCCGCGCCTCCGAGGACGTGCTGATCGAGGACGTGATCCGATGA

Upstream 100 bases:

>100_bases
CGGCGCGCTACGTGCGCGATATCCAGGGCTCCTATTCCAATATCGTCGGCCTGTCGCTGTTCGAGACGGCGCAGTTGCTG
GACGGGTTCGGCTTCGCCCC

Downstream 100 bases:

>100_bases
CCGATGAAACGGCGCCCGCCTGCGCCATCTGCGGCAAGCCCGCCCAGCCCAAATACAAGCCTTTCTGCTCGGCTCGCTGC
GCCGATGTGGACCTGCACCG

Product: ribonuclease G and E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSGRVEILISAGPGEARYLVLADGQPLELVIDRPSLLLDSVFRGRVTALDKGLDAAFVDLGTGPRPGFLPGARALGLSEG
SAVVARVRAEGRGGKGPLLTPAGDFPATGEAPALLRRPDPLDRLRAAFSEAEIVTDAHHEVDEVLQAVLDPVVPLKGGGR
LVIEPAAALTAIDVDSAGARPAEANGAAVTEIARQLRLRGIGGQVVVDFVSGRDRKPLFRLAEALKQAVAADSTPTHVFG
VSPLGLVELTRERRGPALAELLCRRTQAATPETLALAALRALLAESLTRPGKILALRAAPPVAAALAALAAERAEAERRL
GHPLTIREDASRASEDVLIEDVIR

Sequences:

>Translated_344_residues
MSGRVEILISAGPGEARYLVLADGQPLELVIDRPSLLLDSVFRGRVTALDKGLDAAFVDLGTGPRPGFLPGARALGLSEG
SAVVARVRAEGRGGKGPLLTPAGDFPATGEAPALLRRPDPLDRLRAAFSEAEIVTDAHHEVDEVLQAVLDPVVPLKGGGR
LVIEPAAALTAIDVDSAGARPAEANGAAVTEIARQLRLRGIGGQVVVDFVSGRDRKPLFRLAEALKQAVAADSTPTHVFG
VSPLGLVELTRERRGPALAELLCRRTQAATPETLALAALRALLAESLTRPGKILALRAAPPVAAALAALAAERAEAERRL
GHPLTIREDASRASEDVLIEDVIR
>Mature_343_residues
SGRVEILISAGPGEARYLVLADGQPLELVIDRPSLLLDSVFRGRVTALDKGLDAAFVDLGTGPRPGFLPGARALGLSEGS
AVVARVRAEGRGGKGPLLTPAGDFPATGEAPALLRRPDPLDRLRAAFSEAEIVTDAHHEVDEVLQAVLDPVVPLKGGGRL
VIEPAAALTAIDVDSAGARPAEANGAAVTEIARQLRLRGIGGQVVVDFVSGRDRKPLFRLAEALKQAVAADSTPTHVFGV
SPLGLVELTRERRGPALAELLCRRTQAATPETLALAALRALLAESLTRPGKILALRAAPPVAAALAALAAERAEAERRLG
HPLTIREDASRASEDVLIEDVIR

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=209, Percent_Identity=30.622009569378, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87082235, Length=182, Percent_Identity=32.4175824175824, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 35907; Mature: 35776

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRVEILISAGPGEARYLVLADGQPLELVIDRPSLLLDSVFRGRVTALDKGLDAAFVDL
CCCEEEEEEECCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHCHHHHHHCCCCEEEEEC
GTGPRPGFLPGARALGLSEGSAVVARVRAEGRGGKGPLLTPAGDFPATGEAPALLRRPDP
CCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCH
LDRLRAAFSEAEIVTDAHHEVDEVLQAVLDPVVPLKGGGRLVIEPAAALTAIDVDSAGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCCC
PAEANGAAVTEIARQLRLRGIGGQVVVDFVSGRDRKPLFRLAEALKQAVAADSTPTHVFG
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VSPLGLVELTRERRGPALAELLCRRTQAATPETLALAALRALLAESLTRPGKILALRAAP
CCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
PVAAALAALAAERAEAERRLGHPLTIREDASRASEDVLIEDVIR
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGRVEILISAGPGEARYLVLADGQPLELVIDRPSLLLDSVFRGRVTALDKGLDAAFVDL
CCEEEEEEECCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHCHHHHHHCCCCEEEEEC
GTGPRPGFLPGARALGLSEGSAVVARVRAEGRGGKGPLLTPAGDFPATGEAPALLRRPDP
CCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCH
LDRLRAAFSEAEIVTDAHHEVDEVLQAVLDPVVPLKGGGRLVIEPAAALTAIDVDSAGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCCC
PAEANGAAVTEIARQLRLRGIGGQVVVDFVSGRDRKPLFRLAEALKQAVAADSTPTHVFG
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEE
VSPLGLVELTRERRGPALAELLCRRTQAATPETLALAALRALLAESLTRPGKILALRAAP
CCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
PVAAALAALAAERAEAERRLGHPLTIREDASRASEDVLIEDVIR
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]