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The map label for this gene is ygfA [C]

Identifier: 83312323

GI number: 83312323

Start: 3487481

End: 3487921

Strand: Reverse

Name: ygfA [C]

Synonym: amb3224

Alternate gene names: 83312323

Gene position: 3487921-3487481 (Counterclockwise)

Preceding gene: 83312324

Following gene: 83312322

Centisome position: 70.22

GC content: 68.71

Gene sequence:

>441_bases
ATGGTGGTAGCGGGGTATTGGCCGCTGGGCGACGAGATTGACCCGCGGCCCCTGATGGATGCGCTGGCGGCGCGCGGCCA
TGTACTGGCCCTGCCCACCGTGACCCAAAGCGGCGGCATCCTGGAATTCCGCCCGTGGAGCCCCGGAGAGGCGCTGGAGC
CCGGCCCCCACGGCACATGGCACCCCGTCGCCCGCCCCCCCGTCGGTCCGCAAGTCCTGCTGGTGCCGCTACTGGCCTTC
GATACAAGGGGGTTCCGTCTGGGCTATGGCGGCGGCTACTATGACCGGACCTTGGGCCAGTTGCGCCGGGATGGCGCGGT
GTGCGCCATCGGGCTGGCCTTCGCCGCCCAGGAGGTGGACACTGTTCCCACCGACCCCTGGGATATCGCTTTGGATCTCA
TAGCCACGGAGCATGGCGTGATCGTGACGGCCGCTAAATGA

Upstream 100 bases:

>100_bases
ACGGATCGCCCGCCACACGCGGCGTGAGGCGGCGGTGCGCGGCCCGGCCGCGGCCCGGGCCCTGGCGGGGCTGGCCGATG
CCCTGGGACTGGCCCCTGCA

Downstream 100 bases:

>100_bases
GACTTCTGTACATGGGTGACGTGGTCGGCCGTTCGGGCCGCGACGTGATCGTCGGGCGCATGGCCGAGATCAAGAACCGC
CTTTCCCCCGACTTCATCGT

Product: 5-formyltetrahydrofolate cyclo-ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MVVAGYWPLGDEIDPRPLMDALAARGHVLALPTVTQSGGILEFRPWSPGEALEPGPHGTWHPVARPPVGPQVLLVPLLAF
DTRGFRLGYGGGYYDRTLGQLRRDGAVCAIGLAFAAQEVDTVPTDPWDIALDLIATEHGVIVTAAK

Sequences:

>Translated_146_residues
MVVAGYWPLGDEIDPRPLMDALAARGHVLALPTVTQSGGILEFRPWSPGEALEPGPHGTWHPVARPPVGPQVLLVPLLAF
DTRGFRLGYGGGYYDRTLGQLRRDGAVCAIGLAFAAQEVDTVPTDPWDIALDLIATEHGVIVTAAK
>Mature_146_residues
MVVAGYWPLGDEIDPRPLMDALAARGHVLALPTVTQSGGILEFRPWSPGEALEPGPHGTWHPVARPPVGPQVLLVPLLAF
DTRGFRLGYGGGYYDRTLGQLRRDGAVCAIGLAFAAQEVDTVPTDPWDIALDLIATEHGVIVTAAK

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Escherichia coli, GI1789278, Length=144, Percent_Identity=34.0277777777778, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: NA

Molecular weight: Translated: 15558; Mature: 15558

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVAGYWPLGDEIDPRPLMDALAARGHVLALPTVTQSGGILEFRPWSPGEALEPGPHGTW
CEEECCCCCCCCCCCHHHHHHHHHCCCEEEECEECCCCCEEEECCCCCCCCCCCCCCCCC
HPVARPPVGPQVLLVPLLAFDTRGFRLGYGGGYYDRTLGQLRRDGAVCAIGLAFAAQEVD
CCCCCCCCCCHHEEEEHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEHHHHHHHCC
TVPTDPWDIALDLIATEHGVIVTAAK
CCCCCCHHEEEEEEECCCCEEEEECC
>Mature Secondary Structure
MVVAGYWPLGDEIDPRPLMDALAARGHVLALPTVTQSGGILEFRPWSPGEALEPGPHGTW
CEEECCCCCCCCCCCHHHHHHHHHCCCEEEECEECCCCCEEEECCCCCCCCCCCCCCCCC
HPVARPPVGPQVLLVPLLAFDTRGFRLGYGGGYYDRTLGQLRRDGAVCAIGLAFAAQEVD
CCCCCCCCCCHHEEEEHHHHCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEHHHHHHHCC
TVPTDPWDIALDLIATEHGVIVTAAK
CCCCCCHHEEEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]