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The map label for this gene is purA

Identifier: 83312289

GI number: 83312289

Start: 3455514

End: 3456803

Strand: Reverse

Name: purA

Synonym: amb3190

Alternate gene names: 83312289

Gene position: 3456803-3455514 (Counterclockwise)

Preceding gene: 83312290

Following gene: 83312288

Centisome position: 69.59

GC content: 67.98

Gene sequence:

>1290_bases
ATGGCGAACGTGGCTGTGGTCGGCGCCCAGTGGGGCGACGAGGGCAAGGGCAAGGTCGTCGATTGGCTCTCCGAGCGCGC
CGACGTGGTGGTGCGCTTCCAGGGCGGCCATAATGCCGGCCATACCCTGGTCATCAACGGGGTGACCTACAAGCTGTCGC
TGCTGCCTTCGGGCGTGGTGCGCGGCAAGCTGTCCATCATCGGCAACGGCGTGGTGATCGATCCCTGGGCCTTGCTGGCC
GAGATCGAGCGCATCCGCGCCCAGGGGGTGGAGATCACCCCCGAGGTGCTGAAGATCGCCGAGAACGCCTGTCTGATCCT
GCCGCTGCATGCCCATCTGGACAAGGCGCGGGAAGAGGCCAGCGGCACCGCCAAGATCGGCACCACCGGCCGCGGCATCG
GCCCGGCCTACGAGGACCGCGTGGGGCGTCGCGCCATCCGCGTCTGCGATCTGGCCGACGAGGCCGTGCTGAAGGCCAAG
ATCGCCACCCTGCTGCGCCATCACAACGCCCTGCTGCGCGGCCTGGGCGCCGCCGAGGTGGACGGGGCCGAGCTGCTGGC
CAAGCTGCTGGACGTGGCGCCGAAGATCCTGCCCTTCGCCGACGTGGTCTGGCAGCATCTGGATGAGGTGCGCCACACCA
GGAAGCGCGTCCTGTTCGAGGGCGCCCAGGGCGCCATGCTCGACGTCGACCACGGCACCTACCCTTATGTCACCTCGTCC
AACACCGTGTCGGGCAACGCCGGCACCGGCTCGGGCGTCGGGCCGGGGCAGGTGGGCTATGTCCTGGGCATCTGCAAGGC
CTACACCACCCGCGTGGGCGAGGGGCCGTTCCCCACCGAGCTGTTCGACGAGATCGGCAAGAGCATCGGCGAGCGCGGCC
ATGAATTCGGCACCGTCACCGGCCGTCCGCGCCGCTGCGGCTGGTTCGACGCCGTCATGGTCCGCCAGGCGGTCAAGGTG
GGCGGCATCACCGGCATCGCCCTGACCAAGCTGGACGTGCTGGACGGCTTCAAGGAACTGAAGATCTGCACCGGCTACAA
GCTGGATGGCAAGGTGATCAACCATTTCCCGGCCTCCATGACCGGCCAGGCCAATGTGGAGCCCATCTACGAGGTCATCG
AGGGCTGGTCCCAGAGCACCCGTGGCGTGCGGTCGTGGAAGGATCTGCCCGCCACCGCCATCAAGTACATCCGTCGCATC
GAGGAACTGATCGAGGCGCCGGTCGCCCTGCTGTCCACCAGCCCCGAGCGCGAGGACAGCATCCTGGTGCACGATCCCTT
TGCGACTTGA

Upstream 100 bases:

>100_bases
CGCCGCCGACCCGCAAGTGGAAGCAAAGCATCAGGGCTGCAGGCATCGCCTCGGTCCCGATGGTATCGTCGAAGTCGAAT
AGGTTAGGGGACTTAAAGGT

Downstream 100 bases:

>100_bases
TTGTAGGACGGCCTTTCCGCCTTTAGACTGAGAACAGTCCGGCCCCCCACGGGGTCGGCCCATGGAAGGGGCAAATGTCG
GAAATCGACGAGGCCGAGAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MANVAVVGAQWGDEGKGKVVDWLSERADVVVRFQGGHNAGHTLVINGVTYKLSLLPSGVVRGKLSIIGNGVVIDPWALLA
EIERIRAQGVEITPEVLKIAENACLILPLHAHLDKAREEASGTAKIGTTGRGIGPAYEDRVGRRAIRVCDLADEAVLKAK
IATLLRHHNALLRGLGAAEVDGAELLAKLLDVAPKILPFADVVWQHLDEVRHTRKRVLFEGAQGAMLDVDHGTYPYVTSS
NTVSGNAGTGSGVGPGQVGYVLGICKAYTTRVGEGPFPTELFDEIGKSIGERGHEFGTVTGRPRRCGWFDAVMVRQAVKV
GGITGIALTKLDVLDGFKELKICTGYKLDGKVINHFPASMTGQANVEPIYEVIEGWSQSTRGVRSWKDLPATAIKYIRRI
EELIEAPVALLSTSPEREDSILVHDPFAT

Sequences:

>Translated_429_residues
MANVAVVGAQWGDEGKGKVVDWLSERADVVVRFQGGHNAGHTLVINGVTYKLSLLPSGVVRGKLSIIGNGVVIDPWALLA
EIERIRAQGVEITPEVLKIAENACLILPLHAHLDKAREEASGTAKIGTTGRGIGPAYEDRVGRRAIRVCDLADEAVLKAK
IATLLRHHNALLRGLGAAEVDGAELLAKLLDVAPKILPFADVVWQHLDEVRHTRKRVLFEGAQGAMLDVDHGTYPYVTSS
NTVSGNAGTGSGVGPGQVGYVLGICKAYTTRVGEGPFPTELFDEIGKSIGERGHEFGTVTGRPRRCGWFDAVMVRQAVKV
GGITGIALTKLDVLDGFKELKICTGYKLDGKVINHFPASMTGQANVEPIYEVIEGWSQSTRGVRSWKDLPATAIKYIRRI
EELIEAPVALLSTSPEREDSILVHDPFAT
>Mature_428_residues
ANVAVVGAQWGDEGKGKVVDWLSERADVVVRFQGGHNAGHTLVINGVTYKLSLLPSGVVRGKLSIIGNGVVIDPWALLAE
IERIRAQGVEITPEVLKIAENACLILPLHAHLDKAREEASGTAKIGTTGRGIGPAYEDRVGRRAIRVCDLADEAVLKAKI
ATLLRHHNALLRGLGAAEVDGAELLAKLLDVAPKILPFADVVWQHLDEVRHTRKRVLFEGAQGAMLDVDHGTYPYVTSSN
TVSGNAGTGSGVGPGQVGYVLGICKAYTTRVGEGPFPTELFDEIGKSIGERGHEFGTVTGRPRRCGWFDAVMVRQAVKVG
GITGIALTKLDVLDGFKELKICTGYKLDGKVINHFPASMTGQANVEPIYEVIEGWSQSTRGVRSWKDLPATAIKYIRRIE
ELIEAPVALLSTSPEREDSILVHDPFAT

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=43.8388625592417, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.8235294117647, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=41.8781725888325, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=52.5700934579439, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=43.0913348946136, Blast_Score=331, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=43.0913348946136, Blast_Score=331, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=41.5313225058005, Blast_Score=322, Evalue=1e-88,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=43.8388625592417, Blast_Score=337, Evalue=7e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_MAGSA (Q2W2D1)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422553.1
- ProteinModelPortal:   Q2W2D1
- SMR:   Q2W2D1
- STRING:   Q2W2D1
- GeneID:   3806315
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3190
- NMPDR:   fig|342108.5.peg.2861
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   ERDDTIV
- PhylomeDB:   Q2W2D1
- ProtClustDB:   PRK01117
- BioCyc:   MMAG342108:AMB3190-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46136; Mature: 46004

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVAVVGAQWGDEGKGKVVDWLSERADVVVRFQGGHNAGHTLVINGVTYKLSLLPSGVV
CCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEEECCEEEEEECCCCHH
RGKLSIIGNGVVIDPWALLAEIERIRAQGVEITPEVLKIAENACLILPLHAHLDKAREEA
HEEEEEEECCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHC
SGTAKIGTTGRGIGPAYEDRVGRRAIRVCDLADEAVLKAKIATLLRHHNALLRGLGAAEV
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGAELLAKLLDVAPKILPFADVVWQHLDEVRHTRKRVLFEGAQGAMLDVDHGTYPYVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEECC
NTVSGNAGTGSGVGPGQVGYVLGICKAYTTRVGEGPFPTELFDEIGKSIGERGHEFGTVT
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRPRRCGWFDAVMVRQAVKVGGITGIALTKLDVLDGFKELKICTGYKLDGKVINHFPASM
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHEECCCCCCCHHHHCCCCCC
TGQANVEPIYEVIEGWSQSTRGVRSWKDLPATAIKYIRRIEELIEAPVALLSTSPEREDS
CCCCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ILVHDPFAT
EEEECCCCC
>Mature Secondary Structure 
ANVAVVGAQWGDEGKGKVVDWLSERADVVVRFQGGHNAGHTLVINGVTYKLSLLPSGVV
CCEEEEECCCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCEEEEECCEEEEEECCCCHH
RGKLSIIGNGVVIDPWALLAEIERIRAQGVEITPEVLKIAENACLILPLHAHLDKAREEA
HEEEEEEECCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHC
SGTAKIGTTGRGIGPAYEDRVGRRAIRVCDLADEAVLKAKIATLLRHHNALLRGLGAAEV
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGAELLAKLLDVAPKILPFADVVWQHLDEVRHTRKRVLFEGAQGAMLDVDHGTYPYVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEECC
NTVSGNAGTGSGVGPGQVGYVLGICKAYTTRVGEGPFPTELFDEIGKSIGERGHEFGTVT
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRPRRCGWFDAVMVRQAVKVGGITGIALTKLDVLDGFKELKICTGYKLDGKVINHFPASM
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHEECCCCCCCHHHHCCCCCC
TGQANVEPIYEVIEGWSQSTRGVRSWKDLPATAIKYIRRIEELIEAPVALLSTSPEREDS
CCCCCCHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ILVHDPFAT
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA