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The map label for this gene is tuf1

Identifier: 83312231

GI number: 83312231

Start: 3377433

End: 3378623

Strand: Reverse

Name: tuf1

Synonym: amb3132

Alternate gene names: 83312231

Gene position: 3378623-3377433 (Counterclockwise)

Preceding gene: 83312232

Following gene: 162319785

Centisome position: 68.02

GC content: 65.07

Gene sequence:

>1191_bases
ATGGCTAAGGCTAAATTCGAGCGCAACAAGCCTCACTGCAACATCGGGACCATCGGTCACGTTGACCATGGCAAGACCTC
GCTGACCGCGGCGATCACCAAGATCCTGGCCGAGACCGGCGGCGCCACCTTCACCGCCTACGACCAGATCGACAAGGCGC
CGGAAGAGAAGGCCCGTGGCATCACCATCTCGACCGCCCACGTCGAGTACGAGACCAGCAACCGCCACTACGCCCACGTG
GACTGCCCCGGTCACGCCGACTATGTGAAGAACATGATCACCGGCGCGGCGCAGATGGACGGCGCCATCCTGGTGGTGTC
GGCCGCCGACGGCCCCATGCCCCAGACCCGCGAGCACATCCTGCTGGCCCGTCAGGTCGGCGTGCCGGCCCTGGTGGTGT
TCATGAACAAGTGCGACATGGTCGACGATCCCGAGCTGCTGGATCTGGTCGAGCTGGAAGTGCGCGAGCTGCTGAGCTCG
TATGACTTCCCCGGCGACGACATTCCGATCGTGCGCGGTTCGGCCCTGTGTGCCCTGGAAGACAAGCAGCCGGAGATCGG
CCGCGACGCCATCCTGAAGCTGATGGCCGAAGTGGACGCCTACATCCCGCAGCCGGAGCGTCCGAAGGACAAGCCGTTCC
TGATGCCGATCGAAGACGTGTTCTCCATCTCGGGCCGTGGCACCGTGGTGACCGGCCGCGTCGAGCGTGGCGTGGTCAAG
GTGGGCGAGGAAGTCGAGATCGTCGGCATCAAGAACACCGTCAAGACCACCTGCACCGGCGTGGAAATGTTCCGCAAGCT
GCTCGATCAGGGTGAGGCCGGCGACAACATCGGCGCCCTGCTGCGCGGCACCAAGCGCGAGGACGTGGAGCGCGGCCAGG
TTCTGGCGGCTCCGGGCTCCATCACGCCCCACACCGACTTCGAGGCCGAAGCCTACATCCTGAACAAGGAAGAGGGCGGC
CGTCACACCCCGTTCTTCACCAACTACCGTCCGCAGTTCTACTTCCGCACCACCGACGTGACCGGTGTGGTTGCTCTGCC
GGAAGGCACCGAGATGGTGATGCCGGGCGACAACGTGAAGATGATCGTGACCCTGATCGCCCCCATCGCCATGGACCAGG
GCCTGCGCTTCGCCATCCGCGAAGGCGGCCGGACCGTCGGCGCCGGCGTGGTGGCCAAGATCATCAAGTAG

Upstream 100 bases:

>100_bases
CTTTTGACCACTATTCGGAAGTGCCCCAGAACGTGTCGGACGAGATCCGCGCGAAGCTGGCCGGCTAATCTGACACCTAT
CGAGGACGGAGTAAGAAACG

Downstream 100 bases:

>100_bases
TCTTCGGACTTCTGTTTCGGGAAGGGCGTCCGGGTAAAACCGGGCGCCCTTTCCTCTTTTTGGGGAGGCCGGAATGAATC
TCGGCCCGCCCCGCCAAACG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETSNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKCDMVDDPELLDLVELEVRELLSS
YDFPGDDIPIVRGSALCALEDKQPEIGRDAILKLMAEVDAYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGVVK
VGEEVEIVGIKNTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGSITPHTDFEAEAYILNKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVALPEGTEMVMPGDNVKMIVTLIAPIAMDQGLRFAIREGGRTVGAGVVAKIIK

Sequences:

>Translated_396_residues
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETSNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKCDMVDDPELLDLVELEVRELLSS
YDFPGDDIPIVRGSALCALEDKQPEIGRDAILKLMAEVDAYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGVVK
VGEEVEIVGIKNTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGSITPHTDFEAEAYILNKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVALPEGTEMVMPGDNVKMIVTLIAPIAMDQGLRFAIREGGRTVGAGVVAKIIK
>Mature_395_residues
AKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETSNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFMNKCDMVDDPELLDLVELEVRELLSSY
DFPGDDIPIVRGSALCALEDKQPEIGRDAILKLMAEVDAYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGVVKV
GEEVEIVGIKNTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGSITPHTDFEAEAYILNKEEGGR
HTPFFTNYRPQFYFRTTDVTGVVALPEGTEMVMPGDNVKMIVTLIAPIAMDQGLRFAIREGGRTVGAGVVAKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.6142131979695, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=29.1111111111111, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI4503475, Length=349, Percent_Identity=31.8051575931232, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=28.4424379232506, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=28.4424379232506, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI194018522, Length=436, Percent_Identity=27.0642201834862, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194097354, Length=436, Percent_Identity=27.0642201834862, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI194018520, Length=436, Percent_Identity=27.0642201834862, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI46094014, Length=328, Percent_Identity=29.2682926829268, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI54607086, Length=319, Percent_Identity=26.9592476489028, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI4503507, Length=364, Percent_Identity=27.7472527472527, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI157426893, Length=288, Percent_Identity=25.6944444444444, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI53729339, Length=238, Percent_Identity=29.8319327731092, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI53729337, Length=238, Percent_Identity=29.8319327731092, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=78.2828282828283, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=78.2828282828283, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=30.6068601583113, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI48994988, Length=273, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789108, Length=330, Percent_Identity=28.4848484848485, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.3834586466165, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=43.7017994858612, Blast_Score=334, Evalue=6e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=446, Percent_Identity=29.372197309417, Blast_Score=157, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=446, Percent_Identity=29.372197309417, Blast_Score=157, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=29.2343387470998, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=440, Percent_Identity=29.0909090909091, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=427, Percent_Identity=28.8056206088993, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=31.021897810219, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=355, Percent_Identity=28.169014084507, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=231, Percent_Identity=32.034632034632, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=452, Percent_Identity=24.5575221238938, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=26.0683760683761, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=67.3366834170854, Blast_Score=550, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=29.8876404494382, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=29.8876404494382, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=346, Percent_Identity=26.0115606936416, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=28.4057971014493, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=357, Percent_Identity=27.7310924369748, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=27.3109243697479, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=142, Percent_Identity=33.8028169014084, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=60.3053435114504, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=60.3053435114504, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=50.259067357513, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=29.7777777777778, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=29.7777777777778, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=29.7777777777778, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=29.7777777777778, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=29.5045045045045, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=29.5045045045045, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=30.4093567251462, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.0457516339869, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45550900, Length=335, Percent_Identity=27.4626865671642, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24647046, Length=369, Percent_Identity=25.2032520325203, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24647048, Length=369, Percent_Identity=25.2032520325203, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_MAGSA (Q2W2H3)

Other databases:

- EMBL:   AP007255
- EMBL:   AP007255
- RefSeq:   YP_422495.1
- RefSeq:   YP_422511.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q2W2H3
- SMR:   Q2W2H3
- STRING:   Q2W2H3
- GeneID:   3804789
- GeneID:   3804890
- GenomeReviews:   AP007255_GR
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb3132
- KEGG:   mag:amb3148
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q2W2H3
- BioCyc:   MMAG342108:AMB3132-MONOMER
- BioCyc:   MMAG342108:AMB3148-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43054; Mature: 42923

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETSNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFMNKCDMVDDPELLDLVELEVRELLSSYDFPGDDIPIVRGSALCALE
HHHHHCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
DKQPEIGRDAILKLMAEVDAYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGVVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEECCHHCCCEE
VGEEVEIVGIKNTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCEEEECCCC
ITPHTDFEAEAYILNKEEGGRHTPFFTNYRPQFYFRTTDVTGVVALPEGTEMVMPGDNVK
CCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MIVTLIAPIAMDQGLRFAIREGGRTVGAGVVAKIIK
EHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETSNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFMNKCDMVDDPELLDLVELEVRELLSSYDFPGDDIPIVRGSALCALE
HHHHHCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEC
DKQPEIGRDAILKLMAEVDAYIPQPERPKDKPFLMPIEDVFSISGRGTVVTGRVERGVVK
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHCCCCCCEEEECCHHCCCEE
VGEEVEIVGIKNTVKTTCTGVEMFRKLLDQGEAGDNIGALLRGTKREDVERGQVLAAPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCEEEECCCC
ITPHTDFEAEAYILNKEEGGRHTPFFTNYRPQFYFRTTDVTGVVALPEGTEMVMPGDNVK
CCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MIVTLIAPIAMDQGLRFAIREGGRTVGAGVVAKIIK
EHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA