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The map label for this gene is nirT [H]

Identifier: 83311785

GI number: 83311785

Start: 2878350

End: 2878991

Strand: Reverse

Name: nirT [H]

Synonym: amb2686

Alternate gene names: 83311785

Gene position: 2878991-2878350 (Counterclockwise)

Preceding gene: 83311786

Following gene: 83311781

Centisome position: 57.96

GC content: 62.93

Gene sequence:

>642_bases
ATGAGCACTGAGAATCCGGCCAAGCAAGGCGTGTTGAGCGGATGGTGGGCGACGTTGCGCAGGCCCAGCAGCCGCTGGTC
CCTGGGGCTGTTGCTGGTGGTGGGCTTTGTCGGTGGCGTCATGTTCTGGGGCGGCTTCAATACCGTCCTGGAACTGACCA
ATACCGAAGCCTTCTGCCTCTCCTGCCATGAGATGGAGCAGAACGTTTATCGCGAATATCGCGGCACCATCCATGATTCC
AACCGTTCCGGCGTGCGGGCCACCTGCCCGGACTGCCACGTGCCGCGTCCGTGGATCTACAAGATCCAGCGCAAGATCCA
GGCCTCCAACGAGGTGTGGCACAAGATCCTGGGCTCTATCGACACGCGTGAGAAGTTCGAGGCCAAGCGTATCGAACTGG
CCAAGCACGAGTGGGCGCGCATGAAGTCCACGGATTCCCGCGAATGCCGCAACTGCCATAATTTCGGGGCGTTCGACCTG
ACCCGGCAGCAGGCGCGGGCGCGTAACCGTCACTCCGAGGCGGAACAGCAGGGCAAGACCTGCATCGACTGCCACAAGGG
TATCGCCCACCAGCTTCCCGCCGGCGCCTTCAGGGCCGAGCGTGAGCTGAACGAAAGCGTGGGCGGGGCGGTCAAGCCGT
AG

Upstream 100 bases:

>100_bases
GCCATGTGCCGCAGAATGACGCCAAGCCGCTGGTCAACAACAAGTTCCAGGCCGCCACGACCAACACCAATATCCGCTAG
GCAGGGGGAAGGATCGGGCC

Downstream 100 bases:

>100_bases
GGACCACCGCTTGACCCCAATAGCATGGGCCTCCCGAGACTCCCCGGGAGGCCCTTTTTTTGGGCTATTCCCGCGCCATT
TCCTCGTACCAGTCGCGGGG

Product: denitrification system component nirT

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSTENPAKQGVLSGWWATLRRPSSRWSLGLLLVVGFVGGVMFWGGFNTVLELTNTEAFCLSCHEMEQNVYREYRGTIHDS
NRSGVRATCPDCHVPRPWIYKIQRKIQASNEVWHKILGSIDTREKFEAKRIELAKHEWARMKSTDSRECRNCHNFGAFDL
TRQQARARNRHSEAEQQGKTCIDCHKGIAHQLPAGAFRAERELNESVGGAVKP

Sequences:

>Translated_213_residues
MSTENPAKQGVLSGWWATLRRPSSRWSLGLLLVVGFVGGVMFWGGFNTVLELTNTEAFCLSCHEMEQNVYREYRGTIHDS
NRSGVRATCPDCHVPRPWIYKIQRKIQASNEVWHKILGSIDTREKFEAKRIELAKHEWARMKSTDSRECRNCHNFGAFDL
TRQQARARNRHSEAEQQGKTCIDCHKGIAHQLPAGAFRAERELNESVGGAVKP
>Mature_212_residues
STENPAKQGVLSGWWATLRRPSSRWSLGLLLVVGFVGGVMFWGGFNTVLELTNTEAFCLSCHEMEQNVYREYRGTIHDSN
RSGVRATCPDCHVPRPWIYKIQRKIQASNEVWHKILGSIDTREKFEAKRIELAKHEWARMKSTDSRECRNCHNFGAFDLT
RQQARARNRHSEAEQQGKTCIDCHKGIAHQLPAGAFRAERELNESVGGAVKP

Specific function: Mediates Electron Flow From Quinones To The Napab Complex. [C]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788530, Length=186, Percent_Identity=56.989247311828, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1787230, Length=191, Percent_Identity=46.0732984293194, Blast_Score=183, Evalue=7e-48,
Organism=Escherichia coli, GI1788181, Length=168, Percent_Identity=44.047619047619, Blast_Score=152, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005126
- InterPro:   IPR011885 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 24268; Mature: 24137

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTENPAKQGVLSGWWATLRRPSSRWSLGLLLVVGFVGGVMFWGGFNTVLELTNTEAFCL
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHH
SCHEMEQNVYREYRGTIHDSNRSGVRATCPDCHVPRPWIYKIQRKIQASNEVWHKILGSI
HHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
DTREKFEAKRIELAKHEWARMKSTDSRECRNCHNFGAFDLTRQQARARNRHSEAEQQGKT
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCH
CIDCHKGIAHQLPAGAFRAERELNESVGGAVKP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STENPAKQGVLSGWWATLRRPSSRWSLGLLLVVGFVGGVMFWGGFNTVLELTNTEAFCL
CCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHH
SCHEMEQNVYREYRGTIHDSNRSGVRATCPDCHVPRPWIYKIQRKIQASNEVWHKILGSI
HHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
DTREKFEAKRIELAKHEWARMKSTDSRECRNCHNFGAFDLTRQQARARNRHSEAEQQGKT
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCH
CIDCHKGIAHQLPAGAFRAERELNESVGGAVKP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2001732 [H]