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The map label for this gene is yqhE [C]

Identifier: 83311555

GI number: 83311555

Start: 2632919

End: 2633782

Strand: Reverse

Name: yqhE [C]

Synonym: amb2456

Alternate gene names: 83311555

Gene position: 2633782-2632919 (Counterclockwise)

Preceding gene: 83311556

Following gene: 83311554

Centisome position: 53.02

GC content: 65.28

Gene sequence:

>864_bases
ATGGACGGCGCGGTAGACGGAACCTGGACATCCATTCACGGCGTGGCGATCCCCCGGATTCTCTATGGCACGGCCTGGAA
GAAGGACAGGACCGCCGGGCTGGTCGAACTGGCCCTGGCCACGGGTTTTCGCGGCATCGACACCGCCTGCCAGCCCAAAC
ACTACGACGAGGCCGGCGTGGGCGCCGGGCTGGCCGCCGCATTGGCCAAGGGCTTGCGGCGGGAAGACCTTTATCTGCAG
ACCAAATTCACGCCGCTGGCCGGTCAGGACCCCCAGCGCCTTCCTTATGATCCCCAGGCCCCCCTGGCCCGGCAGGTGGC
GCAATCGTTCGAGCGGTCCCGGGCCAATCTTGGCGTCGAGACCCTGGATGGCCTGGTGCTGCATTCGCCTCTGCCCAATC
CCTCCGATTTCCGGGAAGTGTGGGGAGCCATGGAGGCCCTGGTCGATGGCGGCGGGGTGCGGCAACTGGGGATCAGCAAT
TGCTACGATCCCGAGGTGCTGCGCCACCTGTGCGAGACCTCCCGTATCAAGCCGGCCGTGGTCCAGAATCGCTTCTACGC
AGCCACCGGCTTCGATCGCGTCCTGCGCCTCTACTGCCGCCGGAACGGTATTTTCTATCAGAGCTTTTGGACGCTGACCG
CCAACCCCGACATTCTCGCCCATGACGTGACGAGGTCGCTGATGGCCCGGCACGGGCGGAGCGCGGCGCAGATTTTCTTC
CGCTATCTCAGCCAGCAGGAAATCATCCCGCTAACTGGGACGAGTTCGGCCGTGCACATGCGCGAGGATCTGGAGATCGC
GGACTTCGCCCTCTCGGCCGAGGAATGCCAGGCCCTGGAAGCCTTGTTGGGTGGTGGGCCGTAG

Upstream 100 bases:

>100_bases
GGTGCCCTGCCGCGAAGCGGGGCTTTTCCTCAGCCTGTTAGACTTGAATTCGGCGGCCATCGCCCCGACCTAGTCGAGGC
ATCTGGGCAGGGGGCATTCC

Downstream 100 bases:

>100_bases
TGGCGGATGGGGTAGGATTCGAACCCACGGAGGCTTGCGCCCCTCCGGTTTTCAAGACCGGTGCAATAGACCACTCTGCC
ACCCATCCTCTGGGCCGCAG

Product: aldo/keto reductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MDGAVDGTWTSIHGVAIPRILYGTAWKKDRTAGLVELALATGFRGIDTACQPKHYDEAGVGAGLAAALAKGLRREDLYLQ
TKFTPLAGQDPQRLPYDPQAPLARQVAQSFERSRANLGVETLDGLVLHSPLPNPSDFREVWGAMEALVDGGGVRQLGISN
CYDPEVLRHLCETSRIKPAVVQNRFYAATGFDRVLRLYCRRNGIFYQSFWTLTANPDILAHDVTRSLMARHGRSAAQIFF
RYLSQQEIIPLTGTSSAVHMREDLEIADFALSAEECQALEALLGGGP

Sequences:

>Translated_287_residues
MDGAVDGTWTSIHGVAIPRILYGTAWKKDRTAGLVELALATGFRGIDTACQPKHYDEAGVGAGLAAALAKGLRREDLYLQ
TKFTPLAGQDPQRLPYDPQAPLARQVAQSFERSRANLGVETLDGLVLHSPLPNPSDFREVWGAMEALVDGGGVRQLGISN
CYDPEVLRHLCETSRIKPAVVQNRFYAATGFDRVLRLYCRRNGIFYQSFWTLTANPDILAHDVTRSLMARHGRSAAQIFF
RYLSQQEIIPLTGTSSAVHMREDLEIADFALSAEECQALEALLGGGP
>Mature_287_residues
MDGAVDGTWTSIHGVAIPRILYGTAWKKDRTAGLVELALATGFRGIDTACQPKHYDEAGVGAGLAAALAKGLRREDLYLQ
TKFTPLAGQDPQRLPYDPQAPLARQVAQSFERSRANLGVETLDGLVLHSPLPNPSDFREVWGAMEALVDGGGVRQLGISN
CYDPEVLRHLCETSRIKPAVVQNRFYAATGFDRVLRLYCRRNGIFYQSFWTLTANPDILAHDVTRSLMARHGRSAAQIFF
RYLSQQEIIPLTGTSSAVHMREDLEIADFALSAEECQALEALLGGGP

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=300, Percent_Identity=25.3333333333333, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI5174391, Length=300, Percent_Identity=25.3333333333333, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI223468663, Length=290, Percent_Identity=24.4827586206897, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI291291012, Length=274, Percent_Identity=23.3576642335766, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI5174695, Length=304, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310109920, Length=285, Percent_Identity=23.859649122807, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI45446745, Length=285, Percent_Identity=23.859649122807, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4503285, Length=285, Percent_Identity=23.859649122807, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI310109922, Length=267, Percent_Identity=24.3445692883895, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI24497585, Length=284, Percent_Identity=22.887323943662, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI5453543, Length=285, Percent_Identity=23.5087719298246, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4502049, Length=298, Percent_Identity=22.8187919463087, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI24497583, Length=285, Percent_Identity=22.8070175438596, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI87082198, Length=252, Percent_Identity=23.4126984126984, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786400, Length=281, Percent_Identity=27.4021352313167, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17561298, Length=286, Percent_Identity=27.6223776223776, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17552492, Length=282, Percent_Identity=26.5957446808511, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537079, Length=305, Percent_Identity=24.5901639344262, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538386, Length=284, Percent_Identity=26.056338028169, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17537075, Length=304, Percent_Identity=24.3421052631579, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17564128, Length=304, Percent_Identity=25.9868421052632, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71998625, Length=249, Percent_Identity=28.9156626506024, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17537077, Length=301, Percent_Identity=23.5880398671096, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322556, Length=278, Percent_Identity=26.2589928057554, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320079, Length=300, Percent_Identity=23.3333333333333, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24657054, Length=313, Percent_Identity=26.8370607028754, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24663317, Length=301, Percent_Identity=27.906976744186, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20129731, Length=298, Percent_Identity=29.1946308724832, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356425, Length=275, Percent_Identity=26.5454545454545, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45553081, Length=302, Percent_Identity=28.1456953642384, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24644950, Length=308, Percent_Identity=23.3766233766234, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281366140, Length=272, Percent_Identity=27.5735294117647, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24662789, Length=307, Percent_Identity=24.7557003257329, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24662781, Length=282, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24662785, Length=282, Percent_Identity=23.4042553191489, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31456; Mature: 31456

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGAVDGTWTSIHGVAIPRILYGTAWKKDRTAGLVELALATGFRGIDTACQPKHYDEAGV
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GAGLAAALAKGLRREDLYLQTKFTPLAGQDPQRLPYDPQAPLARQVAQSFERSRANLGVE
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHH
TLDGLVLHSPLPNPSDFREVWGAMEALVDGGGVRQLGISNCYDPEVLRHLCETSRIKPAV
HHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCHHH
VQNRFYAATGFDRVLRLYCRRNGIFYQSFWTLTANPDILAHDVTRSLMARHGRSAAQIFF
HHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHH
RYLSQQEIIPLTGTSSAVHMREDLEIADFALSAEECQALEALLGGGP
HHHCCCCEEEECCCCCHHHHHHCCHHHHHHHCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDGAVDGTWTSIHGVAIPRILYGTAWKKDRTAGLVELALATGFRGIDTACQPKHYDEAGV
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
GAGLAAALAKGLRREDLYLQTKFTPLAGQDPQRLPYDPQAPLARQVAQSFERSRANLGVE
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHH
TLDGLVLHSPLPNPSDFREVWGAMEALVDGGGVRQLGISNCYDPEVLRHLCETSRIKPAV
HHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCHHH
VQNRFYAATGFDRVLRLYCRRNGIFYQSFWTLTANPDILAHDVTRSLMARHGRSAAQIFF
HHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHH
RYLSQQEIIPLTGTSSAVHMREDLEIADFALSAEECQALEALLGGGP
HHHCCCCEEEECCCCCHHHHHHCCHHHHHHHCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA