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The map label for this gene is kinA [H]

Identifier: 83311304

GI number: 83311304

Start: 2400688

End: 2402907

Strand: Reverse

Name: kinA [H]

Synonym: amb2205

Alternate gene names: 83311304

Gene position: 2402907-2400688 (Counterclockwise)

Preceding gene: 83311305

Following gene: 83311301

Centisome position: 48.38

GC content: 65.99

Gene sequence:

>2220_bases
ATGGCGCACGGGACCCTCGCGGAAGATGAGCCCGCAGCAAAGCACGGGCGTGCCCCCGGCTCAAGGGGCGGAATTATCCG
GTTGCGCCGTCCCGGGGGCACCGTATCATCGCTTCAAAGCAGGGAGAGGGCGGCCATGTCCAGGGACAGCGACACCAATA
CCGGCAGACGCAGGATTGACCAGGCCGCCAACCTCCTGGTGCGCACCACCGAGGCTTTGCTGCAGGCATCGGACGAACTG
GAACTGCTGGAACAGATCTGCTTGATCGGCATCGAGGCCGGGCACTGCCTCAGCTGGATCGGCTATGCGGGTCGACCTCC
GGAACGGCAGGTGACGGTCATGGCCAGGGCCGGACAGGCGGTGGGCTATCTCGACGACATCGCCATCAGCTGGAACAACG
ACGCCTTTGGCCAGGGTCCCACCGGAACCGCCATCCGCACCGGCCAGCCCTATGTGGTGGCCGACACGAAGACGGACCCG
GCCTTCGCGCCCTGGCGCCACAAGGCCGAGCTTCACGGCTTTCGCTCCGGCGTAGCCCTTCCGCTCGGCGAGGGCGAGCA
GAAGATCGGCGCCTTGATGTACTATGCCACCCAGCCCCAGATGTTCGATGCCCAGGTGGTCGGCATGCTCATGGGCGTGG
CCCGCAATCTGTCCCACGGCATCACCACGCTGCGCATGCGGGCGCGCCACCAGCAGGTGGCCCGGGAACTGGCCGAGAGC
GAGCAAAGGTACCGCTCCCTGGTGGAACTGGCCCCCGACGCCATCGTCGTCCATGGGCATGGCAGCATCCTGTTCGCCAA
TCAGGCGGCCATTCACGCCTTTGGCGCCAGTTCCCGGGCGCCCCTGACCGGAAAGCCGGTCCTGGATCTGGTTCACCCCG
ACAGTCGCGGCGATGCGCTGGAGCGGCTGGAGACCCCGCCTCCCGGCCACATCCTCAATCACTACCGGCTGCTGCGCCTT
GACGGCACGCCTTTCGAGGCCGAGGTGCTGGCCTGCTCCATTTCCTTCAGGGGATTGCCGGCCCGCCTGCTGGTGGTCCG
CGACATCAGCGAGCGCACCCGGGTGCAGGAGACCCTGATGCAGACCGCCAAGCTGGCCACACTGGGTGAAATGGCCGCCG
GGCTGGTCCACGAATTGTCCCAGCCGCTCAACATCATCCGCCTAGCCTCCGAAGGCGCCATGATGCTGATCGAGCGGGAC
AAGGCGACCCAGGAGTTCCAGACCCAGCAATTCGCCTTGATCGCCGAGCAATGCGAACGAACGGCCGAGATCATCGACGA
TATCCGCATCTTTTCGCGCCGTGACACCACGCCCGTCCAGGTCTTCGACGCCGCCCTGGCGGTCCGCTCGGCCATCGAGG
TCCTGGAAGGACAATTCCGCCCCGACGGCATCAGCGTCGACCTTGACCTGCCCATCGGCCCCCTGCCAGTGAGCGGCAGA
CGCATCCAATTGGAGCAGGTCACCATGAACCTGCTGAACAACGCCCATCACGCCCTGCGCGATTCCAAGGATGCCATGCC
GGCCGACTGGATCGGCAAGATCATCGTTCGCGCGAACCGGCAGGGCGATAACGTGGAGATCGTCATCGCCGACAACGGCC
CCGGCATTCCCGATTCCATGAAGGCTCATCTGTTCGAGCCGTTTTTCACCACCAAGGAAGCCGGTCGCGGAACCGGTCTC
GGCCTGTCGGTCAGCCATGGTCTGGTCACCGCCATGAAGGGTGGGCTGATTCTGCGTGAAGGTGGCGAGGGCGCCACCTT
CGTCATCACGCTGCCGCTGGACCTGGAAGCCGCTGGCCCGCAGCCCCTCCCCGCCCTGTCACCCGGCCGGACTCCGGCCG
CCGTATCCGCCGACGCCCATATCCTGGTCGTCGACGACGAAGCCACCGCCGCTGAGACCCTGGCCCGCTACCTGCGCGAG
CTTGGATACAGGGTCAGCATCGCGGGGACGGGCATCGAAGCCTGGGCGCTGTTTTCCGAGGACCCCGCCGATGTGGTCAT
CACCGATTTGCGCATGCCGGCCGGCAATGGCGAGCAATTGGTGGAAAAGCTGCGCGATTTCGACCCGCTGCTGCCCATCG
TCATCGTCACCGGCCACCTGGGCGCCACCGAGAGGGTGGCCGCCAATCTGGAAGACGACCGTTGCGCCGTTCTGAAAAAG
CCCGTTGCCCTGGGGCAGTTGGGCGACCTGGTCACCGTCTTCCTTCAGCCGCCCACGTAG

Upstream 100 bases:

>100_bases
AGGCGGCGGTGTAGGAAGTCTGGACATCCAGGAGATCGAGGGCCGGCTCCGACGCCAGGGCCGCCCCGCCCCATGTGAAC
AAAAACAGCAAGCTGATGAG

Downstream 100 bases:

>100_bases
TTCCCCCGATCGCCCACGTAGTTCCCCCGATCCCTCGAAGACGCTGACGCCATTGTTTGTTCCAAAAAGCTTGTTTATAC
TTTCAGGTAGGTTGGGGATG

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: Stage II sporulation protein F; Stage II sporulation protein J [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MAHGTLAEDEPAAKHGRAPGSRGGIIRLRRPGGTVSSLQSRERAAMSRDSDTNTGRRRIDQAANLLVRTTEALLQASDEL
ELLEQICLIGIEAGHCLSWIGYAGRPPERQVTVMARAGQAVGYLDDIAISWNNDAFGQGPTGTAIRTGQPYVVADTKTDP
AFAPWRHKAELHGFRSGVALPLGEGEQKIGALMYYATQPQMFDAQVVGMLMGVARNLSHGITTLRMRARHQQVARELAES
EQRYRSLVELAPDAIVVHGHGSILFANQAAIHAFGASSRAPLTGKPVLDLVHPDSRGDALERLETPPPGHILNHYRLLRL
DGTPFEAEVLACSISFRGLPARLLVVRDISERTRVQETLMQTAKLATLGEMAAGLVHELSQPLNIIRLASEGAMMLIERD
KATQEFQTQQFALIAEQCERTAEIIDDIRIFSRRDTTPVQVFDAALAVRSAIEVLEGQFRPDGISVDLDLPIGPLPVSGR
RIQLEQVTMNLLNNAHHALRDSKDAMPADWIGKIIVRANRQGDNVEIVIADNGPGIPDSMKAHLFEPFFTTKEAGRGTGL
GLSVSHGLVTAMKGGLILREGGEGATFVITLPLDLEAAGPQPLPALSPGRTPAAVSADAHILVVDDEATAAETLARYLRE
LGYRVSIAGTGIEAWALFSEDPADVVITDLRMPAGNGEQLVEKLRDFDPLLPIVIVTGHLGATERVAANLEDDRCAVLKK
PVALGQLGDLVTVFLQPPT

Sequences:

>Translated_739_residues
MAHGTLAEDEPAAKHGRAPGSRGGIIRLRRPGGTVSSLQSRERAAMSRDSDTNTGRRRIDQAANLLVRTTEALLQASDEL
ELLEQICLIGIEAGHCLSWIGYAGRPPERQVTVMARAGQAVGYLDDIAISWNNDAFGQGPTGTAIRTGQPYVVADTKTDP
AFAPWRHKAELHGFRSGVALPLGEGEQKIGALMYYATQPQMFDAQVVGMLMGVARNLSHGITTLRMRARHQQVARELAES
EQRYRSLVELAPDAIVVHGHGSILFANQAAIHAFGASSRAPLTGKPVLDLVHPDSRGDALERLETPPPGHILNHYRLLRL
DGTPFEAEVLACSISFRGLPARLLVVRDISERTRVQETLMQTAKLATLGEMAAGLVHELSQPLNIIRLASEGAMMLIERD
KATQEFQTQQFALIAEQCERTAEIIDDIRIFSRRDTTPVQVFDAALAVRSAIEVLEGQFRPDGISVDLDLPIGPLPVSGR
RIQLEQVTMNLLNNAHHALRDSKDAMPADWIGKIIVRANRQGDNVEIVIADNGPGIPDSMKAHLFEPFFTTKEAGRGTGL
GLSVSHGLVTAMKGGLILREGGEGATFVITLPLDLEAAGPQPLPALSPGRTPAAVSADAHILVVDDEATAAETLARYLRE
LGYRVSIAGTGIEAWALFSEDPADVVITDLRMPAGNGEQLVEKLRDFDPLLPIVIVTGHLGATERVAANLEDDRCAVLKK
PVALGQLGDLVTVFLQPPT
>Mature_738_residues
AHGTLAEDEPAAKHGRAPGSRGGIIRLRRPGGTVSSLQSRERAAMSRDSDTNTGRRRIDQAANLLVRTTEALLQASDELE
LLEQICLIGIEAGHCLSWIGYAGRPPERQVTVMARAGQAVGYLDDIAISWNNDAFGQGPTGTAIRTGQPYVVADTKTDPA
FAPWRHKAELHGFRSGVALPLGEGEQKIGALMYYATQPQMFDAQVVGMLMGVARNLSHGITTLRMRARHQQVARELAESE
QRYRSLVELAPDAIVVHGHGSILFANQAAIHAFGASSRAPLTGKPVLDLVHPDSRGDALERLETPPPGHILNHYRLLRLD
GTPFEAEVLACSISFRGLPARLLVVRDISERTRVQETLMQTAKLATLGEMAAGLVHELSQPLNIIRLASEGAMMLIERDK
ATQEFQTQQFALIAEQCERTAEIIDDIRIFSRRDTTPVQVFDAALAVRSAIEVLEGQFRPDGISVDLDLPIGPLPVSGRR
IQLEQVTMNLLNNAHHALRDSKDAMPADWIGKIIVRANRQGDNVEIVIADNGPGIPDSMKAHLFEPFFTTKEAGRGTGLG
LSVSHGLVTAMKGGLILREGGEGATFVITLPLDLEAAGPQPLPALSPGRTPAAVSADAHILVVDDEATAAETLARYLREL
GYRVSIAGTGIEAWALFSEDPADVVITDLRMPAGNGEQLVEKLRDFDPLLPIVIVTGHLGATERVAANLEDDRCAVLKKP
VALGQLGDLVTVFLQPPT

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. It also autophosphorylates in the presence of ATP [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=257, Percent_Identity=32.295719844358, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI48994928, Length=417, Percent_Identity=27.3381294964029, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790436, Length=278, Percent_Identity=27.6978417266187, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1788713, Length=229, Percent_Identity=32.7510917030568, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI145693157, Length=380, Percent_Identity=24.7368421052632, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081816, Length=439, Percent_Identity=25.2847380410023, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1786600, Length=381, Percent_Identity=23.0971128608924, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=28.1385281385281, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788393, Length=234, Percent_Identity=26.4957264957265, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI87082128, Length=280, Percent_Identity=27.1428571428571, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR013655
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; PF08447 PAS_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 79766; Mature: 79635

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHGTLAEDEPAAKHGRAPGSRGGIIRLRRPGGTVSSLQSRERAAMSRDSDTNTGRRRID
CCCCCCCCCCCCHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
QAANLLVRTTEALLQASDELELLEQICLIGIEAGHCLSWIGYAGRPPERQVTVMARAGQA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEEECCCC
VGYLDDIAISWNNDAFGQGPTGTAIRTGQPYVVADTKTDPAFAPWRHKAELHGFRSGVAL
CCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCEE
PLGEGEQKIGALMYYATQPQMFDAQVVGMLMGVARNLSHGITTLRMRARHQQVARELAES
ECCCCCHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQRYRSLVELAPDAIVVHGHGSILFANQAAIHAFGASSRAPLTGKPVLDLVHPDSRGDAL
HHHHHHHHHHCCCEEEEECCCCEEEECCCEEEEECCCCCCCCCCCCEEEEECCCCCCCHH
ERLETPPPGHILNHYRLLRLDGTPFEAEVLACSISFRGLPARLLVVRDISERTRVQETLM
HHCCCCCCCCHHCCEEEEEECCCCCEEEEEEEEEECCCCCEEEEEEECHHHHHHHHHHHH
QTAKLATLGEMAAGLVHELSQPLNIIRLASEGAMMLIERDKATQEFQTQQFALIAEQCER
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
TAEIIDDIRIFSRRDTTPVQVFDAALAVRSAIEVLEGQFRPDGISVDLDLPIGPLPVSGR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCC
RIQLEQVTMNLLNNAHHALRDSKDAMPADWIGKIIVRANRQGDNVEIVIADNGPGIPDSM
EEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEHEEECCCCCCEEEEEECCCCCCCCHH
KAHLFEPFFTTKEAGRGTGLGLSVSHGLVTAMKGGLILREGGEGATFVITLPLDLEAAGP
HHHHCCCHHCCCCCCCCCCCCEEHHCCHHHHHCCCEEEEECCCCEEEEEEEEEECCCCCC
QPLPALSPGRTPAAVSADAHILVVDDEATAAETLARYLRELGYRVSIAGTGIEAWALFSE
CCCCCCCCCCCCCEECCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECC
DPADVVITDLRMPAGNGEQLVEKLRDFDPLLPIVIVTGHLGATERVAANLEDDRCAVLKK
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHHHC
PVALGQLGDLVTVFLQPPT
CCHHHCCCCEEEEEEECCC
>Mature Secondary Structure 
AHGTLAEDEPAAKHGRAPGSRGGIIRLRRPGGTVSSLQSRERAAMSRDSDTNTGRRRID
CCCCCCCCCCCHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHH
QAANLLVRTTEALLQASDELELLEQICLIGIEAGHCLSWIGYAGRPPERQVTVMARAGQA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEEECCCC
VGYLDDIAISWNNDAFGQGPTGTAIRTGQPYVVADTKTDPAFAPWRHKAELHGFRSGVAL
CCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHCCCEE
PLGEGEQKIGALMYYATQPQMFDAQVVGMLMGVARNLSHGITTLRMRARHQQVARELAES
ECCCCCHHHCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQRYRSLVELAPDAIVVHGHGSILFANQAAIHAFGASSRAPLTGKPVLDLVHPDSRGDAL
HHHHHHHHHHCCCEEEEECCCCEEEECCCEEEEECCCCCCCCCCCCEEEEECCCCCCCHH
ERLETPPPGHILNHYRLLRLDGTPFEAEVLACSISFRGLPARLLVVRDISERTRVQETLM
HHCCCCCCCCHHCCEEEEEECCCCCEEEEEEEEEECCCCCEEEEEEECHHHHHHHHHHHH
QTAKLATLGEMAAGLVHELSQPLNIIRLASEGAMMLIERDKATQEFQTQQFALIAEQCER
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
TAEIIDDIRIFSRRDTTPVQVFDAALAVRSAIEVLEGQFRPDGISVDLDLPIGPLPVSGR
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCC
RIQLEQVTMNLLNNAHHALRDSKDAMPADWIGKIIVRANRQGDNVEIVIADNGPGIPDSM
EEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEEHEEECCCCCCEEEEEECCCCCCCCHH
KAHLFEPFFTTKEAGRGTGLGLSVSHGLVTAMKGGLILREGGEGATFVITLPLDLEAAGP
HHHHCCCHHCCCCCCCCCCCCEEHHCCHHHHHCCCEEEEECCCCEEEEEEEEEECCCCCC
QPLPALSPGRTPAAVSADAHILVVDDEATAAETLARYLRELGYRVSIAGTGIEAWALFSE
CCCCCCCCCCCCCEECCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECC
DPADVVITDLRMPAGNGEQLVEKLRDFDPLLPIVIVTGHLGATERVAANLEDDRCAVLKK
CCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHHHC
PVALGQLGDLVTVFLQPPT
CCHHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2104615; 2509430; 9384377 [H]