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The map label for this gene is dnaB [H]

Identifier: 83311196

GI number: 83311196

Start: 2272755

End: 2274218

Strand: Reverse

Name: dnaB [H]

Synonym: amb2097

Alternate gene names: 83311196

Gene position: 2274218-2272755 (Counterclockwise)

Preceding gene: 83311197

Following gene: 83311195

Centisome position: 45.79

GC content: 65.3

Gene sequence:

>1464_bases
GTGCCCCCTCACAATTACGAGGCGGAGCAGGCCCTGCTGGGCGCCATCCTGCTGTCCAACCGTGCCTTCGAGCGCGTCTC
GGAATTCCTCAAGCCCGAACACTTCGCCGACCCGGTGCACGGGCGTATCTTCGCCACCTGCGGCAAGCTGATCGAGCGCG
GCCAGATGGCCAACCCGGTGACGCTGAAGACCTGGTTCGAAAACGACGGCGGGCTGGCCGAAATCGGCGGCACCAATTAC
CTCGCCCAACTGGCCAATGCCGTGGTCTCGGTGATCAATGCCGAGGATTACGGCAAGCTGATCTTCGACCTGCATCTGCG
CCGCTCGCTGATCGGCATGGGCGAGGACATGGTCAACGACGCCTTCGCGCCCGATCTGGACGTGCCGGCCATGGACCAGA
TCGGCAAGGCCGAGGCCAAGCTCTATGATCTGGCCACCACCGGCCAGACCGAAGGCGGATTCGAGGATTTCCGAACCGTC
CTGATCAGCGCCGTGGCCAGCGCCGAGGCCGCGCACAAGCGCCAGGGCAAGCTGTCCGGCGTGCCCACCGGCCTGATCGA
CATGGACGCCAAGCTGGGCGGCCTGCACGATTCCGACCTGATCATCCTGGCCGGGCGTCCTTCCATGGGCAAGACCGCCC
TGGCCACCAACATCGCCTTCAACGCCGCCTATGCCTACAAGGAAGAGGTGGACGCCCTGGGCCGCAAGAAGGGCGTCGAC
GGCGCCATCACCGCCTTCTTCTCCCTGGAAATGTCGTCGGAACAGCTGGCGGCCCGTATCCTGGCCGAGCAGGCCGAGAT
CAACAGCCACAAGATCCGCCAGGGCGAGATGTCCAACGAGGAATTCGAGAAGCTGGTGGTCGCCGCCCAGAACCTGCACC
GCCTGCCGTTGTTCATCGACGACACGCCGGCCCTGTCCATCTCGGCGGTGCGCACCCGGGCGCGCCGTCTGCAGCGCCAA
CACGGCCTGGGCCTGATCGTCATCGACTACCTGCAGCTTCTGCGTGGCTCGTCGTCCAATTCCGAGAACCGCGTGCAGGA
AGTGTCGGAGATCACCCGCGGCCTGAAGGCCCTGGCCAAGGAATTGTCGGTGCCGGTGATCGCCCTTTCCCAGTTGTCGC
GCGCCGTCGAGCAGCGCGAGGACAAGCGCCCCCAACTGGCCGATCTGCGCGAATCCGGCTCCATCGAGCAGGACGCCGAC
GTGGTGATGTTCGTGTTCCGCGAACAGTACTACCTGGAACGCGCCGAGCCGGGCCAGCGCCCGGAAGAGGCCCAGGAAAA
GTTCAACGAACGCCACGCCAAGTGGATGGAGCGCTGCGAAGAGGTCCACAACACCGCCGAGGTGATCATCGCCAAGCAGC
GTCACGGCCCGGTCGGCACCGTACGCCTGGCCTTCCAGGGCGAATACACCAAGTTCGGCAACCTGGCGGCCAGCGACCAT
TACGAAGAGCCGCCGGGATACTGA

Upstream 100 bases:

>100_bases
TAAGCCCGCGCAAGCGCAGCGCTTCTGCTAACATGCCCGGGTCATGACCTCGATGCTCCCAGACCATCCCGCCGCCCCCG
CCGAGGGTATCGGCTTTCGC

Downstream 100 bases:

>100_bases
GAAGGAGGCGCATCGTGCCCGACCTGACGTTTCTGGCGGACCCTTATGCCTTCATCCTCCCCGTCCTGGCCCTGTTCGCC
GTGGGGTACAATGTCATCAA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MPPHNYEAEQALLGAILLSNRAFERVSEFLKPEHFADPVHGRIFATCGKLIERGQMANPVTLKTWFENDGGLAEIGGTNY
LAQLANAVVSVINAEDYGKLIFDLHLRRSLIGMGEDMVNDAFAPDLDVPAMDQIGKAEAKLYDLATTGQTEGGFEDFRTV
LISAVASAEAAHKRQGKLSGVPTGLIDMDAKLGGLHDSDLIILAGRPSMGKTALATNIAFNAAYAYKEEVDALGRKKGVD
GAITAFFSLEMSSEQLAARILAEQAEINSHKIRQGEMSNEEFEKLVVAAQNLHRLPLFIDDTPALSISAVRTRARRLQRQ
HGLGLIVIDYLQLLRGSSSNSENRVQEVSEITRGLKALAKELSVPVIALSQLSRAVEQREDKRPQLADLRESGSIEQDAD
VVMFVFREQYYLERAEPGQRPEEAQEKFNERHAKWMERCEEVHNTAEVIIAKQRHGPVGTVRLAFQGEYTKFGNLAASDH
YEEPPGY

Sequences:

>Translated_487_residues
MPPHNYEAEQALLGAILLSNRAFERVSEFLKPEHFADPVHGRIFATCGKLIERGQMANPVTLKTWFENDGGLAEIGGTNY
LAQLANAVVSVINAEDYGKLIFDLHLRRSLIGMGEDMVNDAFAPDLDVPAMDQIGKAEAKLYDLATTGQTEGGFEDFRTV
LISAVASAEAAHKRQGKLSGVPTGLIDMDAKLGGLHDSDLIILAGRPSMGKTALATNIAFNAAYAYKEEVDALGRKKGVD
GAITAFFSLEMSSEQLAARILAEQAEINSHKIRQGEMSNEEFEKLVVAAQNLHRLPLFIDDTPALSISAVRTRARRLQRQ
HGLGLIVIDYLQLLRGSSSNSENRVQEVSEITRGLKALAKELSVPVIALSQLSRAVEQREDKRPQLADLRESGSIEQDAD
VVMFVFREQYYLERAEPGQRPEEAQEKFNERHAKWMERCEEVHNTAEVIIAKQRHGPVGTVRLAFQGEYTKFGNLAASDH
YEEPPGY
>Mature_486_residues
PPHNYEAEQALLGAILLSNRAFERVSEFLKPEHFADPVHGRIFATCGKLIERGQMANPVTLKTWFENDGGLAEIGGTNYL
AQLANAVVSVINAEDYGKLIFDLHLRRSLIGMGEDMVNDAFAPDLDVPAMDQIGKAEAKLYDLATTGQTEGGFEDFRTVL
ISAVASAEAAHKRQGKLSGVPTGLIDMDAKLGGLHDSDLIILAGRPSMGKTALATNIAFNAAYAYKEEVDALGRKKGVDG
AITAFFSLEMSSEQLAARILAEQAEINSHKIRQGEMSNEEFEKLVVAAQNLHRLPLFIDDTPALSISAVRTRARRLQRQH
GLGLIVIDYLQLLRGSSSNSENRVQEVSEITRGLKALAKELSVPVIALSQLSRAVEQREDKRPQLADLRESGSIEQDADV
VMFVFREQYYLERAEPGQRPEEAQEKFNERHAKWMERCEEVHNTAEVIIAKQRHGPVGTVRLAFQGEYTKFGNLAASDHY
EEPPGY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=487, Percent_Identity=37.782340862423, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 53770; Mature: 53638

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPHNYEAEQALLGAILLSNRAFERVSEFLKPEHFADPVHGRIFATCGKLIERGQMANPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCCCCE
TLKTWFENDGGLAEIGGTNYLAQLANAVVSVINAEDYGKLIFDLHLRRSLIGMGEDMVND
EEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHH
AFAPDLDVPAMDQIGKAEAKLYDLATTGQTEGGFEDFRTVLISAVASAEAAHKRQGKLSG
CCCCCCCCCCHHHHCHHHHHHEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPTGLIDMDAKLGGLHDSDLIILAGRPSMGKTALATNIAFNAAYAYKEEVDALGRKKGVD
CCCCCEECCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GAITAFFSLEMSSEQLAARILAEQAEINSHKIRQGEMSNEEFEKLVVAAQNLHRLPLFID
HHEEHHEEECCCHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
DTPALSISAVRTRARRLQRQHGLGLIVIDYLQLLRGSSSNSENRVQEVSEITRGLKALAK
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ELSVPVIALSQLSRAVEQREDKRPQLADLRESGSIEQDADVVMFVFREQYYLERAEPGQR
HHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
PEEAQEKFNERHAKWMERCEEVHNTAEVIIAKQRHGPVGTVRLAFQGEYTKFGNLAASDH
HHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCEEEEEEEECCCCHHCCCCCCCC
YEEPPGY
CCCCCCC
>Mature Secondary Structure 
PPHNYEAEQALLGAILLSNRAFERVSEFLKPEHFADPVHGRIFATCGKLIERGQMANPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHCCCCCCCE
TLKTWFENDGGLAEIGGTNYLAQLANAVVSVINAEDYGKLIFDLHLRRSLIGMGEDMVND
EEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHH
AFAPDLDVPAMDQIGKAEAKLYDLATTGQTEGGFEDFRTVLISAVASAEAAHKRQGKLSG
CCCCCCCCCCHHHHCHHHHHHEEHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPTGLIDMDAKLGGLHDSDLIILAGRPSMGKTALATNIAFNAAYAYKEEVDALGRKKGVD
CCCCCEECCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GAITAFFSLEMSSEQLAARILAEQAEINSHKIRQGEMSNEEFEKLVVAAQNLHRLPLFID
HHEEHHEEECCCHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
DTPALSISAVRTRARRLQRQHGLGLIVIDYLQLLRGSSSNSENRVQEVSEITRGLKALAK
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
ELSVPVIALSQLSRAVEQREDKRPQLADLRESGSIEQDADVVMFVFREQYYLERAEPGQR
HHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
PEEAQEKFNERHAKWMERCEEVHNTAEVIIAKQRHGPVGTVRLAFQGEYTKFGNLAASDH
HHHHHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCEEEEEEEECCCCHHCCCCCCCC
YEEPPGY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA