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The map label for this gene is insF1 [H]

Identifier: 83311140

GI number: 83311140

Start: 2219115

End: 2219972

Strand: Reverse

Name: insF1 [H]

Synonym: amb2041

Alternate gene names: 83311140

Gene position: 2219972-2219115 (Counterclockwise)

Preceding gene: 83311141

Following gene: 83311135

Centisome position: 44.69

GC content: 62.12

Gene sequence:

>858_bases
GTGAGGCGGACCATGATCGAGCCCAAACACCCGGCGCTGTCGATCGGCGCCCAGTGCCACCTGCTGTCCATTGCGCGATC
ATCGTTCTATTACGAGCCCTTGGGCGAGACGGAGGCAAACCTGGAGCTGATGCGGTTGATCGACCGCCAATTCCTGGAAA
CGCCGTTCTATGGTGTCCGTCAGATGGCTTGGCATCTCCAGAACGAGGGCCACCCGGTCAACGTCAAGCGCGTCCGGCGG
CTGATGCGGCTCATGGGCCTGATGCCGATCTACCAGAAGCCCAACACCAGCAAGCCCGCCAAGGGCCACAAGACCTATCC
CTACCTGCTGCGGGGCCTGCGGATCGACCGGCCCAATCAGGTCTGGTGCGCCGACATCACCTATCTGCCCATGCGCCGGG
GATTCCTTTATCTGATCGCCATCATGGACTGGGCCACCCGGAAAGTGCTGGCCTGGCGCATCTCCAACACGCTCGAGGCC
GACTTCTGCGTCGAGGTGCTCAACGAGGCCATCCTGCGCTTCGGCCCGCCGGAGATCATGAACACCGACCAGGGCAGCCA
GTTTACATCCTTCGCCTGGATCGACCGGCTGAAGCGGGCCGGAACCCGCATCTCCATGGACGGCAAGGGCCGTTGCATCG
ACAACGTCTTTATCGAGCGCCTGTGGCGGTCCCTAAAATACGAATGCGTCTATCTCCATGCCTGGGAAACCGGCTCGCAA
GCAAGGGCCGGGATCGGACCCTGGATCACCTTCTACAACCACAAACGGCCCCACGCCGCCCATGGCGGAAGGACCCCCGC
CGTGGTCTACTGGAACACCATCAACCAAACCGATCAGCAGGCCCGGAGAGTAGCTTAA

Upstream 100 bases:

>100_bases
AGGTGGACGAGGATACGGTGCGATCCCTGCACGCCAAGATCGGGGAGCTGGCCGTGGCCAACGATTTTTTGTCTCGAAAG
CTCAAGCCGTGGACCGGCAA

Downstream 100 bases:

>100_bases
ACTTCCCCGAATTCTGTCCAACCAATGGGGAGTAGCTCAATCTTCACCATCAAGCTTCAGCCTCTGATCGGCGGTGTCTG
GTGCATTCAGGACGGCTTTA

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MRRTMIEPKHPALSIGAQCHLLSIARSSFYYEPLGETEANLELMRLIDRQFLETPFYGVRQMAWHLQNEGHPVNVKRVRR
LMRLMGLMPIYQKPNTSKPAKGHKTYPYLLRGLRIDRPNQVWCADITYLPMRRGFLYLIAIMDWATRKVLAWRISNTLEA
DFCVEVLNEAILRFGPPEIMNTDQGSQFTSFAWIDRLKRAGTRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQ
ARAGIGPWITFYNHKRPHAAHGGRTPAVVYWNTINQTDQQARRVA

Sequences:

>Translated_285_residues
MRRTMIEPKHPALSIGAQCHLLSIARSSFYYEPLGETEANLELMRLIDRQFLETPFYGVRQMAWHLQNEGHPVNVKRVRR
LMRLMGLMPIYQKPNTSKPAKGHKTYPYLLRGLRIDRPNQVWCADITYLPMRRGFLYLIAIMDWATRKVLAWRISNTLEA
DFCVEVLNEAILRFGPPEIMNTDQGSQFTSFAWIDRLKRAGTRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQ
ARAGIGPWITFYNHKRPHAAHGGRTPAVVYWNTINQTDQQARRVA
>Mature_285_residues
MRRTMIEPKHPALSIGAQCHLLSIARSSFYYEPLGETEANLELMRLIDRQFLETPFYGVRQMAWHLQNEGHPVNVKRVRR
LMRLMGLMPIYQKPNTSKPAKGHKTYPYLLRGLRIDRPNQVWCADITYLPMRRGFLYLIAIMDWATRKVLAWRISNTLEA
DFCVEVLNEAILRFGPPEIMNTDQGSQFTSFAWIDRLKRAGTRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQ
ARAGIGPWITFYNHKRPHAAHGGRTPAVVYWNTINQTDQQARRVA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=237, Percent_Identity=29.535864978903, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1788405, Length=279, Percent_Identity=30.4659498207885, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787263, Length=279, Percent_Identity=30.4659498207885, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786753, Length=279, Percent_Identity=30.4659498207885, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786570, Length=279, Percent_Identity=30.4659498207885, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786490, Length=279, Percent_Identity=30.4659498207885, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789981, Length=251, Percent_Identity=29.4820717131474, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33223; Mature: 33223

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTMIEPKHPALSIGAQCHLLSIARSSFYYEPLGETEANLELMRLIDRQFLETPFYGVR
CCCCCCCCCCCCEECCCHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHCCCHHHHH
QMAWHLQNEGHPVNVKRVRRLMRLMGLMPIYQKPNTSKPAKGHKTYPYLLRGLRIDRPNQ
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
VWCADITYLPMRRGFLYLIAIMDWATRKVLAWRISNTLEADFCVEVLNEAILRFGPPEIM
EEEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHC
NTDQGSQFTSFAWIDRLKRAGTRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQ
CCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCC
ARAGIGPWITFYNHKRPHAAHGGRTPAVVYWNTINQTDQQARRVA
CCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCC
>Mature Secondary Structure
MRRTMIEPKHPALSIGAQCHLLSIARSSFYYEPLGETEANLELMRLIDRQFLETPFYGVR
CCCCCCCCCCCCEECCCHHHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHCCCHHHHH
QMAWHLQNEGHPVNVKRVRRLMRLMGLMPIYQKPNTSKPAKGHKTYPYLLRGLRIDRPNQ
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
VWCADITYLPMRRGFLYLIAIMDWATRKVLAWRISNTLEADFCVEVLNEAILRFGPPEIM
EEEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHC
NTDQGSQFTSFAWIDRLKRAGTRISMDGKGRCIDNVFIERLWRSLKYECVYLHAWETGSQ
CCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCC
ARAGIGPWITFYNHKRPHAAHGGRTPAVVYWNTINQTDQQARRVA
CCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]