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The map label for this gene is braE [H]

Identifier: 83311127

GI number: 83311127

Start: 2205501

End: 2206490

Strand: Reverse

Name: braE [H]

Synonym: amb2028

Alternate gene names: 83311127

Gene position: 2206490-2205501 (Counterclockwise)

Preceding gene: 83311128

Following gene: 83311126

Centisome position: 44.42

GC content: 65.96

Gene sequence:

>990_bases
ATGAGCGCGCTGCTGCAAGACCGCCGTGCCGTCCTGCTGCTGGGCCTGCTGGCGATTCTCGCCCTGGCGCCGCTGGCGGC
GGGCGACTATGTGCTGTCCATCCTGATCACCGTGCTGCTCCAGGCCTATCTCGGCATGAGCTGGAACGTGATGATGGGCT
TTGCCGGCCAGTTCTCGCTGGGCCACGCCCTCTATGTGGGGCTGGGGGCCTATGCCGGCGGCGCCCTGTTCGTCCATTAC
GGCACGCCGCCCTGGATCGGTGCCCTGGCCGGCATGGCGGTCTCGGCCGCGGTGGGTGCCGTGGTCGGCACACTCGGCTT
CCGCTTCAAGGTGATGGGCGTCTATTTCGCCCTGCTGACCATCGCCTTCGCCGAGTTCACCCGGGTGCTGTTCGACCATT
GGGGCTGGGTGGGGGCCTCGTCGGGCCTGTTCCTGCCCCCCGCCCCCAACAACAGCCCGGACAATCTGTGGCTGCTGCGC
GGCTCGACCACCATGTTCTACTACGTGGCGCTGGCCATGGCGGCGGGGGCCTTCTGGTTCAGCCGCGCCCTGCTGATGCG
CCGCATCGGCTTTTACTGGCAGGCCATCCGCGAGGATCAGGACGCCGCCGACGCGCTGGGCATTGATGTCTTCAAGAACA
AGATGATCGCCGTGCTGATCTCGGCCTCCATGACCAGCCTGGGCGGCGTGTTCCTGGCCTTCTTCAACAACAACCTCTAC
CCCGAGAGCATTTTTTCCATGCATCGCTCCATCGAGTTGATGCTGGGCACCATCATCGGGGGAATCGGCACCCTGTTCGG
GCCGATCCTGGGCGCCGTGGTGTTGACCGCCCTGGGCGAGGGCCTGACCATCCTGGGCGCCCATATCGGCATCGATGGCG
TCAAGCAGATCGCCTACGGCATCTGTCTGATGCTTATCCTGGTCCTGAAGCCGGGCGGGCTGTGGCCCTGGCTGCGCAAG
GCCTTCCGCCTGGAGGGAGACGGACGATGA

Upstream 100 bases:

>100_bases
CGGGCGTGCTGATCGCTCCGTCGCTCAAAAGCATGTTCAGCTTCGGCCTGCTGATCCTGGTCCTGCTGCTGCGGCCGCAA
GGCATCCTGGGGAAGAAGCC

Downstream 100 bases:

>100_bases
ATACCGCCACTCCCATGCTTTCGGTCGAGAACCTGGGCAAGAGCTTCCGCGGCCTGCGCGCCGTCAACCAGGTGGCCTTC
GAGGTGCCGCGCGGCGACAT

Product: ABC-type branched-chain amino acid transport system

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSALLQDRRAVLLLGLLAILALAPLAAGDYVLSILITVLLQAYLGMSWNVMMGFAGQFSLGHALYVGLGAYAGGALFVHY
GTPPWIGALAGMAVSAAVGAVVGTLGFRFKVMGVYFALLTIAFAEFTRVLFDHWGWVGASSGLFLPPAPNNSPDNLWLLR
GSTTMFYYVALAMAAGAFWFSRALLMRRIGFYWQAIREDQDAADALGIDVFKNKMIAVLISASMTSLGGVFLAFFNNNLY
PESIFSMHRSIELMLGTIIGGIGTLFGPILGAVVLTALGEGLTILGAHIGIDGVKQIAYGICLMLILVLKPGGLWPWLRK
AFRLEGDGR

Sequences:

>Translated_329_residues
MSALLQDRRAVLLLGLLAILALAPLAAGDYVLSILITVLLQAYLGMSWNVMMGFAGQFSLGHALYVGLGAYAGGALFVHY
GTPPWIGALAGMAVSAAVGAVVGTLGFRFKVMGVYFALLTIAFAEFTRVLFDHWGWVGASSGLFLPPAPNNSPDNLWLLR
GSTTMFYYVALAMAAGAFWFSRALLMRRIGFYWQAIREDQDAADALGIDVFKNKMIAVLISASMTSLGGVFLAFFNNNLY
PESIFSMHRSIELMLGTIIGGIGTLFGPILGAVVLTALGEGLTILGAHIGIDGVKQIAYGICLMLILVLKPGGLWPWLRK
AFRLEGDGR
>Mature_328_residues
SALLQDRRAVLLLGLLAILALAPLAAGDYVLSILITVLLQAYLGMSWNVMMGFAGQFSLGHALYVGLGAYAGGALFVHYG
TPPWIGALAGMAVSAAVGAVVGTLGFRFKVMGVYFALLTIAFAEFTRVLFDHWGWVGASSGLFLPPAPNNSPDNLWLLRG
STTMFYYVALAMAAGAFWFSRALLMRRIGFYWQAIREDQDAADALGIDVFKNKMIAVLISASMTSLGGVFLAFFNNNLYP
ESIFSMHRSIELMLGTIIGGIGTLFGPILGAVVLTALGEGLTILGAHIGIDGVKQIAYGICLMLILVLKPGGLWPWLRKA
FRLEGDGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=322, Percent_Identity=24.5341614906832, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35223; Mature: 35092

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLQDRRAVLLLGLLAILALAPLAAGDYVLSILITVLLQAYLGMSWNVMMGFAGQFSL
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHH
GHALYVGLGAYAGGALFVHYGTPPWIGALAGMAVSAAVGAVVGTLGFRFKVMGVYFALLT
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFAEFTRVLFDHWGWVGASSGLFLPPAPNNSPDNLWLLRGSTTMFYYVALAMAAGAFWF
HHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
SRALLMRRIGFYWQAIREDQDAADALGIDVFKNKMIAVLISASMTSLGGVFLAFFNNNLY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PESIFSMHRSIELMLGTIIGGIGTLFGPILGAVVLTALGEGLTILGAHIGIDGVKQIAYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCHHHHHHHHHH
ICLMLILVLKPGGLWPWLRKAFRLEGDGR
HHHHHHHHHCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SALLQDRRAVLLLGLLAILALAPLAAGDYVLSILITVLLQAYLGMSWNVMMGFAGQFSL
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHH
GHALYVGLGAYAGGALFVHYGTPPWIGALAGMAVSAAVGAVVGTLGFRFKVMGVYFALLT
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAFAEFTRVLFDHWGWVGASSGLFLPPAPNNSPDNLWLLRGSTTMFYYVALAMAAGAFWF
HHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
SRALLMRRIGFYWQAIREDQDAADALGIDVFKNKMIAVLISASMTSLGGVFLAFFNNNLY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PESIFSMHRSIELMLGTIIGGIGTLFGPILGAVVLTALGEGLTILGAHIGIDGVKQIAYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHCHHHHHHHHHH
ICLMLILVLKPGGLWPWLRKAFRLEGDGR
HHHHHHHHHCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]