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The map label for this gene is luxQ [H]

Identifier: 83311055

GI number: 83311055

Start: 2109003

End: 2110547

Strand: Reverse

Name: luxQ [H]

Synonym: amb1956

Alternate gene names: 83311055

Gene position: 2110547-2109003 (Counterclockwise)

Preceding gene: 83311057

Following gene: 83311053

Centisome position: 42.49

GC content: 67.12

Gene sequence:

>1545_bases
ATGCATCACGGGGCAGGGTCTGCGGAGTTCAAGTGGCGCCACCTCATCGAGGCGGTGGAAGAGTTCGGTCAAGGCTTTAC
CGTATTCGACCGCGACCTGAATCTGATCTTGTGCAACAACCGCTTCCTGGAAATGCTGGACTTCCCCCCCAGCCTGATTC
ACCCGGGCACGGTCTTCGCCGACTTCATGCGCTATAACGCCATGCGGGGCGAGTACGGCCCCGGCGACGTGGAGGCCCTG
GTGGCCGAGCGGGTGGAGCGGGCCCGCAATTTCGCCCCCCATTGCTTCGAGCGCGAACGCCCCGACGGCACGGTGGTCGA
AGTGCGCGGCACACCGCTTCCCGGTGGCGGCTTCGTCACCACCTACACCGACATCACCGACCGCAAGCGTGCCGAGCGCA
TGCTGATCCACGCCAAGGACAAGGCGGAGGAGACCGCCCGGCTCAAGGCCAACTTCCTGGCCACCATGAGCCACGAGATC
CGCACGCCCATGAACGGGGTGCTGGGCATGCTGCACCTGCTGACCGGCTCGCCCCTGACGCCAGATCAGCGCGACCACCT
GGAGACGGCCCAATCCTCGGCCCGCGCCCTGCTGGCCATCATCAACGACATCCTGGACTTCTCCAAGCTGGAGGCCGGGC
AGATGAAGCTGGAGGCCACCAAATTCGACCTGCATCGCCTGCTGGAGGAGATGGTGGCGTTGCTGAAGGGGGCGGCGCGC
GACAAGGGGCTGACGCTGAGCCTGCGCATCGCCGACACCGTGCCGCGCTATCAGGCGGGCGACCCGACCCGCCTGCGCCA
GGTGCTGACCAATCTGATGGGCAACGCCGTCAAGTTTACCGAGAAGGGGGATGTGGCCGTCTCGGTCGAGCCGGAGGAAT
CCGACTCCTCCCGCCTGCGCTTCGAAGTGGCCGATACCGGCATCGGCATCGAACCCGCCGCCGCCCCCGCCCTGTTCTCC
GAGTTCGTCCAGGCGGATTCCTCCATCACCCGGCGCTTTGGCGGCACCGGCCTGGGGCTGTCCATCTGTAAGAGGCTGGT
GGAGATGATGGGGGGCGAGATCGGCTTCGACAGCATCCCCGGCGAAGGAACCACCTTCCATTTCACCCTACCGCTGAGCG
CCGCCCTGCCCGACCAGGACTCAGGGGCGGCGGAGGATGCCGGCCCCGACCTGCCGCCCATGGCGGTTCTGCTGGCCGAG
GACAACCCGGTCAACCAGAAGCTGACCACCACATTGCTGGATCGCTGGGGCCAAAGGGTCACCCTGGCCCGCAACGGTGC
CGAAGCCATCGCCGCAGTGGCCCGCGATCATTTCGACCTGGTGCTGATGGACGTCCACATGCCCGGCATCGACGGTCTGG
AGGCGACGCGGCGCATCCGGGCCATGAGCGGCCCGGTGGCCAAAATCCCCATCATCGCCATGACGGCCGACGTGATGGAT
GGCGATGCCGCCCTCTGCCTGGACGCCGGCATGGACGACTACGTGGCCAAGCCGGTGGAGCCCACCCGCCTGCTGGCGGC
GCTGCACCGCGCCATCAGGCGCTGA

Upstream 100 bases:

>100_bases
TGGTTACAAAGGTATAGATCCTTCCACCGGCGAGCACGCCGCTTCCGCCCTGGCGGAATTTATCATATGCTACATTTGCG
CGAGCGGAGGGGACCGGGGC

Downstream 100 bases:

>100_bases
CAGGCGGAGGAAAATCCGCTTGCGCGCAGTGCTACCGCCCTGACCCGTTCGGAGGCACAGCCTCCGAACCACCAGTTTTT
TTATTTCAATGAGTTAAAAA

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MHHGAGSAEFKWRHLIEAVEEFGQGFTVFDRDLNLILCNNRFLEMLDFPPSLIHPGTVFADFMRYNAMRGEYGPGDVEAL
VAERVERARNFAPHCFERERPDGTVVEVRGTPLPGGGFVTTYTDITDRKRAERMLIHAKDKAEETARLKANFLATMSHEI
RTPMNGVLGMLHLLTGSPLTPDQRDHLETAQSSARALLAIINDILDFSKLEAGQMKLEATKFDLHRLLEEMVALLKGAAR
DKGLTLSLRIADTVPRYQAGDPTRLRQVLTNLMGNAVKFTEKGDVAVSVEPEESDSSRLRFEVADTGIGIEPAAAPALFS
EFVQADSSITRRFGGTGLGLSICKRLVEMMGGEIGFDSIPGEGTTFHFTLPLSAALPDQDSGAAEDAGPDLPPMAVLLAE
DNPVNQKLTTTLLDRWGQRVTLARNGAEAIAAVARDHFDLVLMDVHMPGIDGLEATRRIRAMSGPVAKIPIIAMTADVMD
GDAALCLDAGMDDYVAKPVEPTRLLAALHRAIRR

Sequences:

>Translated_514_residues
MHHGAGSAEFKWRHLIEAVEEFGQGFTVFDRDLNLILCNNRFLEMLDFPPSLIHPGTVFADFMRYNAMRGEYGPGDVEAL
VAERVERARNFAPHCFERERPDGTVVEVRGTPLPGGGFVTTYTDITDRKRAERMLIHAKDKAEETARLKANFLATMSHEI
RTPMNGVLGMLHLLTGSPLTPDQRDHLETAQSSARALLAIINDILDFSKLEAGQMKLEATKFDLHRLLEEMVALLKGAAR
DKGLTLSLRIADTVPRYQAGDPTRLRQVLTNLMGNAVKFTEKGDVAVSVEPEESDSSRLRFEVADTGIGIEPAAAPALFS
EFVQADSSITRRFGGTGLGLSICKRLVEMMGGEIGFDSIPGEGTTFHFTLPLSAALPDQDSGAAEDAGPDLPPMAVLLAE
DNPVNQKLTTTLLDRWGQRVTLARNGAEAIAAVARDHFDLVLMDVHMPGIDGLEATRRIRAMSGPVAKIPIIAMTADVMD
GDAALCLDAGMDDYVAKPVEPTRLLAALHRAIRR
>Mature_514_residues
MHHGAGSAEFKWRHLIEAVEEFGQGFTVFDRDLNLILCNNRFLEMLDFPPSLIHPGTVFADFMRYNAMRGEYGPGDVEAL
VAERVERARNFAPHCFERERPDGTVVEVRGTPLPGGGFVTTYTDITDRKRAERMLIHAKDKAEETARLKANFLATMSHEI
RTPMNGVLGMLHLLTGSPLTPDQRDHLETAQSSARALLAIINDILDFSKLEAGQMKLEATKFDLHRLLEEMVALLKGAAR
DKGLTLSLRIADTVPRYQAGDPTRLRQVLTNLMGNAVKFTEKGDVAVSVEPEESDSSRLRFEVADTGIGIEPAAAPALFS
EFVQADSSITRRFGGTGLGLSICKRLVEMMGGEIGFDSIPGEGTTFHFTLPLSAALPDQDSGAAEDAGPDLPPMAVLLAE
DNPVNQKLTTTLLDRWGQRVTLARNGAEAIAAVARDHFDLVLMDVHMPGIDGLEATRRIRAMSGPVAKIPIIAMTADVMD
GDAALCLDAGMDDYVAKPVEPTRLLAALHRAIRR

Specific function: At low cell density, in absence of AI-2 (autoinducer 2), luxQ has a kinase activity and autophosphorylates on a histidine residue. The phosphoryl group is then transferred to an aspartate residue in the response regulator domain. The phosphoryl group is t

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=375, Percent_Identity=41.0666666666667, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI48994928, Length=450, Percent_Identity=34.6666666666667, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1788713, Length=403, Percent_Identity=33.0024813895782, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87081816, Length=350, Percent_Identity=36.8571428571429, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI145693157, Length=266, Percent_Identity=39.8496240601504, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=30.3571428571429, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788549, Length=258, Percent_Identity=26.3565891472868, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788393, Length=236, Percent_Identity=27.5423728813559, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790436, Length=247, Percent_Identity=29.1497975708502, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786783, Length=236, Percent_Identity=28.3898305084746, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=25.9842519685039, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082128, Length=229, Percent_Identity=28.3842794759825, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787894, Length=241, Percent_Identity=29.4605809128631, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790300, Length=278, Percent_Identity=26.6187050359712, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=33.6134453781513, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788394, Length=119, Percent_Identity=34.453781512605, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790346, Length=227, Percent_Identity=27.3127753303965, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790551, Length=212, Percent_Identity=30.188679245283, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=34.1880341880342, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789808, Length=252, Percent_Identity=25.7936507936508, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788545, Length=187, Percent_Identity=26.7379679144385, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=28.9256198347107, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=75, Percent_Identity=49.3333333333333, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322000, Length=153, Percent_Identity=34.640522875817, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR015387
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789
- ProDom:   PD142495 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF09308 LuxQ-periplasm; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56146; Mature: 56146

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHGAGSAEFKWRHLIEAVEEFGQGFTVFDRDLNLILCNNRFLEMLDFPPSLIHPGTVFA
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCHHHHHCCCHHHCCCHHHHH
DFMRYNAMRGEYGPGDVEALVAERVERARNFAPHCFERERPDGTVVEVRGTPLPGGGFVT
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCEEE
TYTDITDRKRAERMLIHAKDKAEETARLKANFLATMSHEIRTPMNGVLGMLHLLTGSPLT
EECCHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PDQRDHLETAQSSARALLAIINDILDFSKLEAGQMKLEATKFDLHRLLEEMVALLKGAAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
DKGLTLSLRIADTVPRYQAGDPTRLRQVLTNLMGNAVKFTEKGDVAVSVEPEESDSSRLR
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCEEE
FEVADTGIGIEPAAAPALFSEFVQADSSITRRFGGTGLGLSICKRLVEMMGGEIGFDSIP
EEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCC
GEGTTFHFTLPLSAALPDQDSGAAEDAGPDLPPMAVLLAEDNPVNQKLTTTLLDRWGQRV
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEE
TLARNGAEAIAAVARDHFDLVLMDVHMPGIDGLEATRRIRAMSGPVAKIPIIAMTADVMD
EEECCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCC
GDAALCLDAGMDDYVAKPVEPTRLLAALHRAIRR
CCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MHHGAGSAEFKWRHLIEAVEEFGQGFTVFDRDLNLILCNNRFLEMLDFPPSLIHPGTVFA
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCHHHHHCCCHHHCCCHHHHH
DFMRYNAMRGEYGPGDVEALVAERVERARNFAPHCFERERPDGTVVEVRGTPLPGGGFVT
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCEEE
TYTDITDRKRAERMLIHAKDKAEETARLKANFLATMSHEIRTPMNGVLGMLHLLTGSPLT
EECCHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PDQRDHLETAQSSARALLAIINDILDFSKLEAGQMKLEATKFDLHRLLEEMVALLKGAAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
DKGLTLSLRIADTVPRYQAGDPTRLRQVLTNLMGNAVKFTEKGDVAVSVEPEESDSSRLR
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCEEE
FEVADTGIGIEPAAAPALFSEFVQADSSITRRFGGTGLGLSICKRLVEMMGGEIGFDSIP
EEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCC
GEGTTFHFTLPLSAALPDQDSGAAEDAGPDLPPMAVLLAEDNPVNQKLTTTLLDRWGQRV
CCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEE
TLARNGAEAIAAVARDHFDLVLMDVHMPGIDGLEATRRIRAMSGPVAKIPIIAMTADVMD
EEECCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCC
GDAALCLDAGMDDYVAKPVEPTRLLAALHRAIRR
CCCEEEEECCCHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301; 12176318 [H]