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The map label for this gene is pilR [H]

Identifier: 83311013

GI number: 83311013

Start: 2061688

End: 2063019

Strand: Reverse

Name: pilR [H]

Synonym: amb1914

Alternate gene names: 83311013

Gene position: 2063019-2061688 (Counterclockwise)

Preceding gene: 83311014

Following gene: 83311007

Centisome position: 41.53

GC content: 68.62

Gene sequence:

>1332_bases
ATGAGCAAGCTCCTCCTGGTCGATGACGAGCCCGCCTTTCTTCGCCTCGCTGCCGCTTGGCTGGAAAAGCAGGGGCACAG
TGTGGAGACCGCCGCCGATGGCGCCCGAGCCCGTGAAGCCTTTCTCCGCGAGCGCCCCGATCTGGTGCTGCTCGATCTGG
TGATGCCGCCCGAGCGCACGCCGGAAGCGGGACTGGCGCGGATACCGGACTTCGCCGCCGTACCGGTGGTGGTGCTGACC
GCCCATGCCGATCATGACCTGGCGTTGCGGGCGGTGGCGGCGGGGGCCTGGGATTTCCTGGCCAAGCCGGTGGAACCAGA
CCTGCTGCGTTTCGTGGTCGAGCTGGCCTTGCTCAAGCGCGGCCTGGAGCAGGAAGTGGCGTCCTTGCGCGCCGCCCAGC
CGACCGAGGACGGCATCTTCGGTTCCAGTCCGGTCATCCTGCGCCTCAAGGAGATGATCCGCCGTATCGCTCCCGCCGAC
CTGCCGGTGATGGTGCTGGGACCCAGCGGCACCGGCAAGGAACTGGTGGCCCATGCCCTTCATGCCGCCAGTCCGCGCCG
CTCTGGTCCCTTCGTCGCCGTTCATTGCGGTGCCATTCCGGCGGAACTGCTGGAAAGCGAGTTGTTCGGCCACCTGAAGG
ACAGCTTCACCGGCGCCCATCAGGATCGCCAGGGGCTGGTAGCGGCGGCTCATGGCGGCACCCTGTTCCCCGATGAGGTG
GGCGAGATGCCACCATCCATGCAGGTCAAGCTGCTGCGCTTCCTGCAGGAAGACACCTATAGCCCGGTGGGCGGACGCCA
GCCCCTGACCGCCGATGTGCGGGTGGTCTCGGCCACCCACCGTGATATCGAGGCCATGGCGGTCGAGGGCTCGTTCCGCG
AAGACTTCTATTACCGTCTCAAGGGCCTGATCCTGCGCAGCCCGCCGCTGGCCGAACGCCGCGAGGACATTGCCCCCTTG
GCCGCCCGCTTCCTGGCCGATGCCGCCAAGCGCCGACGGCTGCATCTGACGGCGGAAGCCCTGGCGTGGTTGGCCGAGCA
GGACTGGCCGGGCAATGTCCGTGAACTCAAGGCGGTAGTGACCTCCGCCGCCGCCCTGGCCGAAGGAAGCGTCCTCACGC
TGGCTGATCTCGCCTTCGCCCGCAGCGGCGATCCCGCCACCCTCCCCCAGGCCGCGCCCGCCACCCTGGCCGAGACCGTG
GCCGATCTCGAGCGCCGTCTGATCATCACGGCGCTTGCCGCCACCGGCAACAACCACAGCGCCGCCGCCTGCCGCCTCGG
CCTGTCGCGGGTAGGGTTGCTGAAGATGATGACGCGGCTGGGGTTGCGGTAA

Upstream 100 bases:

>100_bases
GGTTGGCCATCGTCGCCAAGGTGATGGCGGCCCATAATGGTTCGGTGCTCTTGAGCGAACGTCCCAGTTGGTCCACCTGT
TTCAGCTTGAGGTTCCCGGC

Downstream 100 bases:

>100_bases
CTTCGTCCAAAACCATCCCCTAACCATCTGATTTCATTGAGGCGGAAGACGCCGTGTCGCAACGTCTCCAACCGCAACAA
ATTTTGACCACCCCATTGAA

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSKLLLVDDEPAFLRLAAAWLEKQGHSVETAADGARAREAFLRERPDLVLLDLVMPPERTPEAGLARIPDFAAVPVVVLT
AHADHDLALRAVAAGAWDFLAKPVEPDLLRFVVELALLKRGLEQEVASLRAAQPTEDGIFGSSPVILRLKEMIRRIAPAD
LPVMVLGPSGTGKELVAHALHAASPRRSGPFVAVHCGAIPAELLESELFGHLKDSFTGAHQDRQGLVAAAHGGTLFPDEV
GEMPPSMQVKLLRFLQEDTYSPVGGRQPLTADVRVVSATHRDIEAMAVEGSFREDFYYRLKGLILRSPPLAERREDIAPL
AARFLADAAKRRRLHLTAEALAWLAEQDWPGNVRELKAVVTSAAALAEGSVLTLADLAFARSGDPATLPQAAPATLAETV
ADLERRLIITALAATGNNHSAAACRLGLSRVGLLKMMTRLGLR

Sequences:

>Translated_443_residues
MSKLLLVDDEPAFLRLAAAWLEKQGHSVETAADGARAREAFLRERPDLVLLDLVMPPERTPEAGLARIPDFAAVPVVVLT
AHADHDLALRAVAAGAWDFLAKPVEPDLLRFVVELALLKRGLEQEVASLRAAQPTEDGIFGSSPVILRLKEMIRRIAPAD
LPVMVLGPSGTGKELVAHALHAASPRRSGPFVAVHCGAIPAELLESELFGHLKDSFTGAHQDRQGLVAAAHGGTLFPDEV
GEMPPSMQVKLLRFLQEDTYSPVGGRQPLTADVRVVSATHRDIEAMAVEGSFREDFYYRLKGLILRSPPLAERREDIAPL
AARFLADAAKRRRLHLTAEALAWLAEQDWPGNVRELKAVVTSAAALAEGSVLTLADLAFARSGDPATLPQAAPATLAETV
ADLERRLIITALAATGNNHSAAACRLGLSRVGLLKMMTRLGLR
>Mature_442_residues
SKLLLVDDEPAFLRLAAAWLEKQGHSVETAADGARAREAFLRERPDLVLLDLVMPPERTPEAGLARIPDFAAVPVVVLTA
HADHDLALRAVAAGAWDFLAKPVEPDLLRFVVELALLKRGLEQEVASLRAAQPTEDGIFGSSPVILRLKEMIRRIAPADL
PVMVLGPSGTGKELVAHALHAASPRRSGPFVAVHCGAIPAELLESELFGHLKDSFTGAHQDRQGLVAAAHGGTLFPDEVG
EMPPSMQVKLLRFLQEDTYSPVGGRQPLTADVRVVSATHRDIEAMAVEGSFREDFYYRLKGLILRSPPLAERREDIAPLA
ARFLADAAKRRRLHLTAEALAWLAEQDWPGNVRELKAVVTSAAALAEGSVLTLADLAFARSGDPATLPQAAPATLAETVA
DLERRLIITALAATGNNHSAAACRLGLSRVGLLKMMTRLGLR

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=454, Percent_Identity=37.0044052863436, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1788905, Length=440, Percent_Identity=41.3636363636364, Blast_Score=291, Evalue=8e-80,
Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=39.4557823129252, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=37.1794871794872, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1789087, Length=312, Percent_Identity=41.025641025641, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87082117, Length=347, Percent_Identity=39.193083573487, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1789233, Length=234, Percent_Identity=44.4444444444444, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI87082152, Length=317, Percent_Identity=39.4321766561514, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786524, Length=320, Percent_Identity=39.375, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=36.4516129032258, Blast_Score=185, Evalue=7e-48,
Organism=Escherichia coli, GI87081872, Length=309, Percent_Identity=37.8640776699029, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789828, Length=256, Percent_Identity=35.546875, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081858, Length=302, Percent_Identity=29.1390728476821, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 47664; Mature: 47532

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLLLVDDEPAFLRLAAAWLEKQGHSVETAADGARAREAFLRERPDLVLLDLVMPPERT
CCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHCHHHHHHHHHHCCCCEEEEEEECCCCCC
PEAGLARIPDFAAVPVVVLTAHADHDLALRAVAAGAWDFLAKPVEPDLLRFVVELALLKR
CCCCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GLEQEVASLRAAQPTEDGIFGSSPVILRLKEMIRRIAPADLPVMVLGPSGTGKELVAHAL
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
HAASPRRSGPFVAVHCGAIPAELLESELFGHLKDSFTGAHQDRQGLVAAAHGGTLFPDEV
HHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHH
GEMPPSMQVKLLRFLQEDTYSPVGGRQPLTADVRVVSATHRDIEAMAVEGSFREDFYYRL
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCEEEEECCCHHHHHHHHH
KGLILRSPPLAERREDIAPLAARFLADAAKRRRLHLTAEALAWLAEQDWPGNVRELKAVV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCCCHHHHHHHH
TSAAALAEGSVLTLADLAFARSGDPATLPQAAPATLAETVADLERRLIITALAATGNNHS
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCC
AAACRLGLSRVGLLKMMTRLGLR
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKLLLVDDEPAFLRLAAAWLEKQGHSVETAADGARAREAFLRERPDLVLLDLVMPPERT
CCEEEECCCCHHHHHHHHHHHHCCCCCCHHHCHHHHHHHHHHCCCCEEEEEEECCCCCC
PEAGLARIPDFAAVPVVVLTAHADHDLALRAVAAGAWDFLAKPVEPDLLRFVVELALLKR
CCCCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GLEQEVASLRAAQPTEDGIFGSSPVILRLKEMIRRIAPADLPVMVLGPSGTGKELVAHAL
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
HAASPRRSGPFVAVHCGAIPAELLESELFGHLKDSFTGAHQDRQGLVAAAHGGTLFPDEV
HHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHH
GEMPPSMQVKLLRFLQEDTYSPVGGRQPLTADVRVVSATHRDIEAMAVEGSFREDFYYRL
HCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHCCCCEEEEECCCHHHHHHHHH
KGLILRSPPLAERREDIAPLAARFLADAAKRRRLHLTAEALAWLAEQDWPGNVRELKAVV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCCCHHHHHHHH
TSAAALAEGSVLTLADLAFARSGDPATLPQAAPATLAETVADLERRLIITALAATGNNHS
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCC
AAACRLGLSRVGLLKMMTRLGLR
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]