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The map label for this gene is gatA [H]

Identifier: 83310767

GI number: 83310767

Start: 1805886

End: 1807061

Strand: Reverse

Name: gatA [H]

Synonym: amb1668

Alternate gene names: 83310767

Gene position: 1807061-1805886 (Counterclockwise)

Preceding gene: 83310768

Following gene: 83310764

Centisome position: 36.38

GC content: 70.92

Gene sequence:

>1176_bases
ATGCATGATCCGTTGAACGCGTTCGCTCTGGGTGGTGACGTCTATCTGAACGGGGCCGAGACCGGCCCGCTGGCTGGCCT
CACCTTCGCCGCCAAGGACGTCTTCGACGTCGCCGGCTATGTCACCGGGGCGGGCAATCCCGACTGGCGCCGCCTGGCCG
AGCCGGCCAAGCACACCGCCTGGGCCATCGGCACCTTGCTGGAGTCCGGCGCCCGGCTGGTGGGCAAGACCCATACCGAC
GAGCTGACCCGCGGCATCTTCGGCGACAATTCCCATTACGGCACCCCCGACAACCCCCGCGCCCCCGGCCATGTGCCGGG
CGGTTCCTCCAGCGGATCGGCGGCGGCCGTGGCCGGCGGCCTGTGCTCCATGGCGCTGGGCACCGACACCGCCGGATCGA
CCCGCGTGCCGGCCTCGTTCTGCGGCGTGTTCGGCCTGCGCCCCACGCTCGGCACCATCCCCATGGACGGCGTGCTGAGC
CAGTCCAACACCTTCGACACCGTCGGCCTGCTGGCCGATGACCCCGAGATTCTCGCCAAGATGGGCGAGGCGCTGCTGCG
CAAGAAGATCAAGGACACCCGCCCTGCCCAGGTGGTGGTGCTGGAAGACGCCCTGGAAGCCTCGGACCCGGCCGTCGCCG
CCGCCATCGAGGCCGCCCTGCCCCGCATCAAGGAGGCCGTCGCCCCCATCGTGCGGGGCCGCAAGCTCTCGCCCGTGCCG
CTGGCCGACTGGGTCGAGCACCAGAACGCCATCCAGGGCCGCGAGGCCTGGGAGACCTTCGGCGAGTGGATCAACAATTC
CAATCCCCGTTTCGGCTTCGAGGTAGCGGACAACTTCCTGCGCGGCTCCAAGGTCTCGCAGCGCACCCTCTCGGCGGCCC
GCGGCTTCCGCCTGCGCGCCAAGCGCTGGGTGCAGGAGGCGCTGGAGGGCAATGCCATGCTGGTGCTGCCCACCACGCCG
GTGACCGCGCCGCCGGTGCATTCCCCCCGTTCGGTCATGTGGGAAATCCGCCACCGCATCGTCAGCCTGACCACCATCGC
CGGCATGGCCGGCTGCCCGCAGATCAGCCTGCCGCTGTGCAAGGTCGACGGCCTGCCGGTGGGTCTGTCGCTGATCGGCC
CGCGCGGCTCTGACGCCCTGCTGCTGGCGGCGGCGCAGCGGATCGGCAAGGCCTGA

Upstream 100 bases:

>100_bases
AGCAGCCCGGCAATGCCGCCAAGGGACTGGACCCCGCCCGGTGATCTGGTATTTTCCCTGACAGGCCGCAATCGCGGCGA
CGTTGTAGGGGCCTTCTGTC

Downstream 100 bases:

>100_bases
GCCCTTCGGGGCTCCCGCCCCCCGATCTGGGAAAGATGGCCCCGGTTGGAGGCGATGCCTCCAAACCTCTCTTTTATTTG
CAAATATAAAGGGGGTCCGG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MHDPLNAFALGGDVYLNGAETGPLAGLTFAAKDVFDVAGYVTGAGNPDWRRLAEPAKHTAWAIGTLLESGARLVGKTHTD
ELTRGIFGDNSHYGTPDNPRAPGHVPGGSSSGSAAAVAGGLCSMALGTDTAGSTRVPASFCGVFGLRPTLGTIPMDGVLS
QSNTFDTVGLLADDPEILAKMGEALLRKKIKDTRPAQVVVLEDALEASDPAVAAAIEAALPRIKEAVAPIVRGRKLSPVP
LADWVEHQNAIQGREAWETFGEWINNSNPRFGFEVADNFLRGSKVSQRTLSAARGFRLRAKRWVQEALEGNAMLVLPTTP
VTAPPVHSPRSVMWEIRHRIVSLTTIAGMAGCPQISLPLCKVDGLPVGLSLIGPRGSDALLLAAAQRIGKA

Sequences:

>Translated_391_residues
MHDPLNAFALGGDVYLNGAETGPLAGLTFAAKDVFDVAGYVTGAGNPDWRRLAEPAKHTAWAIGTLLESGARLVGKTHTD
ELTRGIFGDNSHYGTPDNPRAPGHVPGGSSSGSAAAVAGGLCSMALGTDTAGSTRVPASFCGVFGLRPTLGTIPMDGVLS
QSNTFDTVGLLADDPEILAKMGEALLRKKIKDTRPAQVVVLEDALEASDPAVAAAIEAALPRIKEAVAPIVRGRKLSPVP
LADWVEHQNAIQGREAWETFGEWINNSNPRFGFEVADNFLRGSKVSQRTLSAARGFRLRAKRWVQEALEGNAMLVLPTTP
VTAPPVHSPRSVMWEIRHRIVSLTTIAGMAGCPQISLPLCKVDGLPVGLSLIGPRGSDALLLAAAQRIGKA
>Mature_391_residues
MHDPLNAFALGGDVYLNGAETGPLAGLTFAAKDVFDVAGYVTGAGNPDWRRLAEPAKHTAWAIGTLLESGARLVGKTHTD
ELTRGIFGDNSHYGTPDNPRAPGHVPGGSSSGSAAAVAGGLCSMALGTDTAGSTRVPASFCGVFGLRPTLGTIPMDGVLS
QSNTFDTVGLLADDPEILAKMGEALLRKKIKDTRPAQVVVLEDALEASDPAVAAAIEAALPRIKEAVAPIVRGRKLSPVP
LADWVEHQNAIQGREAWETFGEWINNSNPRFGFEVADNFLRGSKVSQRTLSAARGFRLRAKRWVQEALEGNAMLVLPTTP
VTAPPVHSPRSVMWEIRHRIVSLTTIAGMAGCPQISLPLCKVDGLPVGLSLIGPRGSDALLLAAAQRIGKA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=449, Percent_Identity=26.9487750556793, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI195972892, Length=411, Percent_Identity=23.3576642335766, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=24.822695035461, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=416, Percent_Identity=25.7211538461538, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=128, Percent_Identity=35.9375, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=392, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=433, Percent_Identity=25.1732101616628, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356731, Length=403, Percent_Identity=23.5732009925558, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648113, Length=155, Percent_Identity=36.7741935483871, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45550774, Length=154, Percent_Identity=31.8181818181818, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 41049; Mature: 41049

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDPLNAFALGGDVYLNGAETGPLAGLTFAAKDVFDVAGYVTGAGNPDWRRLAEPAKHTA
CCCCCCCEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
WAIGTLLESGARLVGKTHTDELTRGIFGDNSHYGTPDNPRAPGHVPGGSSSGSAAAVAGG
HHHHHHHHHCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LCSMALGTDTAGSTRVPASFCGVFGLRPTLGTIPMDGVLSQSNTFDTVGLLADDPEILAK
HHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
MGEALLRKKIKDTRPAQVVVLEDALEASDPAVAAAIEAALPRIKEAVAPIVRGRKLSPVP
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LADWVEHQNAIQGREAWETFGEWINNSNPRFGFEVADNFLRGSKVSQRTLSAARGFRLRA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHCCCHHHHHHHHHHHCHHHHH
KRWVQEALEGNAMLVLPTTPVTAPPVHSPRSVMWEIRHRIVSLTTIAGMAGCPQISLPLC
HHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
KVDGLPVGLSLIGPRGSDALLLAAAQRIGKA
EECCCCCCEEEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MHDPLNAFALGGDVYLNGAETGPLAGLTFAAKDVFDVAGYVTGAGNPDWRRLAEPAKHTA
CCCCCCCEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
WAIGTLLESGARLVGKTHTDELTRGIFGDNSHYGTPDNPRAPGHVPGGSSSGSAAAVAGG
HHHHHHHHHCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LCSMALGTDTAGSTRVPASFCGVFGLRPTLGTIPMDGVLSQSNTFDTVGLLADDPEILAK
HHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHH
MGEALLRKKIKDTRPAQVVVLEDALEASDPAVAAAIEAALPRIKEAVAPIVRGRKLSPVP
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LADWVEHQNAIQGREAWETFGEWINNSNPRFGFEVADNFLRGSKVSQRTLSAARGFRLRA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHCCCHHHHHHHHHHHCHHHHH
KRWVQEALEGNAMLVLPTTPVTAPPVHSPRSVMWEIRHRIVSLTTIAGMAGCPQISLPLC
HHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
KVDGLPVGLSLIGPRGSDALLLAAAQRIGKA
EECCCCCCEEEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]