Click here to switch to the map view.

The map label for this gene is hybO [H]

Identifier: 83310749

GI number: 83310749

Start: 1787615

End: 1788745

Strand: Reverse

Name: hybO [H]

Synonym: amb1650

Alternate gene names: 83310749

Gene position: 1788745-1787615 (Counterclockwise)

Preceding gene: 83310751

Following gene: 83310748

Centisome position: 36.01

GC content: 66.49

Gene sequence:

>1131_bases
ATGTACGACGATACCGAAATTTCCCGCGAGATCGCGAACCGGTTCAATCTTGAACGCCGCGACTTCCTGAAGTTCTGCGC
CGCCATGGCCGCGACCATGGGCCTGCCCAAAGGGGCGGAGGCCGAGATCGCCCAGGCCATCACCGCCAAGGAGCGCCCCA
GCGTGATCTGGCTGCACTTCCAGGAGTGTACCGGCTGCACCGAATCCATGCTCCGCGCCGAGCATCCGACCATCGAGAAG
CTGATCCTGGAGATCATCTCCCTCGATTATCACGAGACGCTGTTCGCCGCCGCCGGCCATCAGGTCGAGGCCGCCCGCGA
AGCCGCCATGAAGAAGAACTGGGGCAAGTACGTCCTGGTCATCGAAGGCGCCATCCCGGTCAAGGACGGCGGCATCTACT
GCAAGGTGGGCGGCAAGACCGCGCTGCAGCTGACCAAGGAATGCGCCGAGGGCGCGGCGGCCGTGATCGCCATCGGCTCC
TGCGCCTCGTGGGGCGGCATGCCGTCCACGCCGCCGAACCCCACGGGCTCGACCGGCGTGCCGGAGATCCTGGCGCCCAA
GCCGGTGGTGACCATCCCCGGCTGCCCGCCCAACCCCTATAACTTCCTGTCCACCGTGGTCCACTTCCTGACCTTCGGCA
GCCTGCCGGCGGTGGACGACAAGGGCCGCCCGAAATTCGCCTATTCCCGTCTCATCCACGAGAATTGCGAGCGCCGCGCC
CATTTCGACGCCGGCCGCTTCGCGCTGGAATTCGGCGACGAGGGCCATCGCAAGGGCTATTGCCTGTACAAGCTGGGCTG
CAAGGGTCCCGAGACCTACGCCAATTGCCCGGCCATCCTGTTCAACGACGTGGGTTCGGGCTCGTGGCCGGTGGGCACCG
GGCATCCCTGCATCGGCTGCACGGAAAAGGGCATCGGCTTCCACAAGCCCATCCACGCCCTGGCCGAGTTGCAGAACCAG
CGTCCGCCCGTGGGCTATCCCCGCGTGGTCGAGGAGCAGGGCGTCGGAGCCTCCATCGGCGCCATCGCCACGGTGGCGGC
GGTCGGTGGCCTGGCCATCGGTGCCGGCGCCAAGCTGGTGGGCAATCTGGGCAAGAGTGAAAACGGCCAGGATTCCGGGA
AACAGGGGTAA

Upstream 100 bases:

>100_bases
GACTGAGCGGCAGGGGCATGACCCCCAGGCCCGCGCATGGCTCCGCGATCACCCCTCCCGGACTTTCGACAGCCCGACGC
GCCAGCCAAGGTGGGGGACA

Downstream 100 bases:

>100_bases
TCCGCCATGACCATCACCAGACGCAATTTCCTGGCGACGGCGGCGGCGGGCGGAACGGCGGCAGCGGCCGCCTGCGCCAC
CCTGGCCCCCGCCGGCGCCG

Product: Ni,Fe-hydrogenase I small subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MYDDTEISREIANRFNLERRDFLKFCAAMAATMGLPKGAEAEIAQAITAKERPSVIWLHFQECTGCTESMLRAEHPTIEK
LILEIISLDYHETLFAAAGHQVEAAREAAMKKNWGKYVLVIEGAIPVKDGGIYCKVGGKTALQLTKECAEGAAAVIAIGS
CASWGGMPSTPPNPTGSTGVPEILAPKPVVTIPGCPPNPYNFLSTVVHFLTFGSLPAVDDKGRPKFAYSRLIHENCERRA
HFDAGRFALEFGDEGHRKGYCLYKLGCKGPETYANCPAILFNDVGSGSWPVGTGHPCIGCTEKGIGFHKPIHALAELQNQ
RPPVGYPRVVEEQGVGASIGAIATVAAVGGLAIGAGAKLVGNLGKSENGQDSGKQG

Sequences:

>Translated_376_residues
MYDDTEISREIANRFNLERRDFLKFCAAMAATMGLPKGAEAEIAQAITAKERPSVIWLHFQECTGCTESMLRAEHPTIEK
LILEIISLDYHETLFAAAGHQVEAAREAAMKKNWGKYVLVIEGAIPVKDGGIYCKVGGKTALQLTKECAEGAAAVIAIGS
CASWGGMPSTPPNPTGSTGVPEILAPKPVVTIPGCPPNPYNFLSTVVHFLTFGSLPAVDDKGRPKFAYSRLIHENCERRA
HFDAGRFALEFGDEGHRKGYCLYKLGCKGPETYANCPAILFNDVGSGSWPVGTGHPCIGCTEKGIGFHKPIHALAELQNQ
RPPVGYPRVVEEQGVGASIGAIATVAAVGGLAIGAGAKLVGNLGKSENGQDSGKQG
>Mature_376_residues
MYDDTEISREIANRFNLERRDFLKFCAAMAATMGLPKGAEAEIAQAITAKERPSVIWLHFQECTGCTESMLRAEHPTIEK
LILEIISLDYHETLFAAAGHQVEAAREAAMKKNWGKYVLVIEGAIPVKDGGIYCKVGGKTALQLTKECAEGAAAVIAIGS
CASWGGMPSTPPNPTGSTGVPEILAPKPVVTIPGCPPNPYNFLSTVVHFLTFGSLPAVDDKGRPKFAYSRLIHENCERRA
HFDAGRFALEFGDEGHRKGYCLYKLGCKGPETYANCPAILFNDVGSGSWPVGTGHPCIGCTEKGIGFHKPIHALAELQNQ
RPPVGYPRVVEEQGVGASIGAIATVAAVGGLAIGAGAKLVGNLGKSENGQDSGKQG

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side. Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=360, Percent_Identity=57.2222222222222, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1787206, Length=303, Percent_Identity=43.5643564356436, Blast_Score=265, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 39934; Mature: 39934

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDDTEISREIANRFNLERRDFLKFCAAMAATMGLPKGAEAEIAQAITAKERPSVIWLHF
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEE
QECTGCTESMLRAEHPTIEKLILEIISLDYHETLFAAAGHQVEAAREAAMKKNWGKYVLV
HHHCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEE
IEGAIPVKDGGIYCKVGGKTALQLTKECAEGAAAVIAIGSCASWGGMPSTPPNPTGSTGV
EECCEEEECCCEEEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
PEILAPKPVVTIPGCPPNPYNFLSTVVHFLTFGSLPAVDDKGRPKFAYSRLIHENCERRA
CHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HFDAGRFALEFGDEGHRKGYCLYKLGCKGPETYANCPAILFNDVGSGSWPVGTGHPCIGC
CCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHCCCEEEEECCCCCCCCCCCCCCCCCC
TEKGIGFHKPIHALAELQNQRPPVGYPRVVEEQGVGASIGAIATVAAVGGLAIGAGAKLV
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHH
GNLGKSENGQDSGKQG
HCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYDDTEISREIANRFNLERRDFLKFCAAMAATMGLPKGAEAEIAQAITAKERPSVIWLHF
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEE
QECTGCTESMLRAEHPTIEKLILEIISLDYHETLFAAAGHQVEAAREAAMKKNWGKYVLV
HHHCCHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEE
IEGAIPVKDGGIYCKVGGKTALQLTKECAEGAAAVIAIGSCASWGGMPSTPPNPTGSTGV
EECCEEEECCCEEEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCC
PEILAPKPVVTIPGCPPNPYNFLSTVVHFLTFGSLPAVDDKGRPKFAYSRLIHENCERRA
CHHCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
HFDAGRFALEFGDEGHRKGYCLYKLGCKGPETYANCPAILFNDVGSGSWPVGTGHPCIGC
CCCCCCEEEEECCCCCCCCEEEEEECCCCCCCHHCCCEEEEECCCCCCCCCCCCCCCCCC
TEKGIGFHKPIHALAELQNQRPPVGYPRVVEEQGVGASIGAIATVAAVGGLAIGAGAKLV
CCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHH
GNLGKSENGQDSGKQG
HCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]