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The map label for this gene is hypE [H]

Identifier: 83310735

GI number: 83310735

Start: 1776475

End: 1777524

Strand: Reverse

Name: hypE [H]

Synonym: amb1636

Alternate gene names: 83310735

Gene position: 1777524-1776475 (Counterclockwise)

Preceding gene: 83310736

Following gene: 83310734

Centisome position: 35.79

GC content: 67.43

Gene sequence:

>1050_bases
ATGACCCTGCCCCGCCGCGCCCATCCCCGCCCGCTGGACATCGCCTCCGGCCGCGTCGACCTCACCCATGGCGGCGGCGG
CCGCGCCATGGCCCAACTGGTCGAGGAATTGTTCATCGCCGCCTTCGACAACGAGGCGCTGAACCAGCGCAATGACCAGG
CCGCCTTCGACGTTGCCGGCGGCAGGATGGTGATGACCACCGACGGCTACGTCATCAGCCCGCTGTTCTTTCCCGGCGGC
GACATCGGGTCGCTGGCCGTGCACGGCACCGTCAACGACGTGGCCATGGCCGGCGCCCGGCCCATCTCGCTGTCGGCGGG
CTTCATCATCGAGGAGGGTTTTCCCCTCTCGGACCTGAAGCGCATCGTCGAGTCCATGGCCAAGGCCAGCAAGGAGGCCG
GCGTGCCGGTGGTCACCGGCGACACCAAGGTGGTTGAAAAGGGCAAGGGCGACGGCATCTTCATCACCACGGCGGGCATC
GGCATGGTGCCGCCCGGCGTGGAGATTTCCGGCGACAAGGCGCGGTCCGGCGACGCCATCCTGGTGTCTGGCACCATGGG
CGACCACGGCGTGGCGGTGATGAGCCAGCGGGAAAGCTTGGGATTCGAGACCGAGATCCTGTCCGATTCCGCCGCATTGC
ATACAATGGTCGCCGAGATGGTCGCAGCGGTCCCGGGTATCCGGGTGCTTCGCGATCCCACAAGGGGCGGCCTTGCCGCC
ACCTTGAACGAGATCGCCAGCCAGTCGGGAATCGGCATGCTGATGGAAGAGGCTTTGGTGCCGGTCAAGCCCCAGGTGCT
GGGCGCCTGCGAGCTGCTGGGTCTGGACCCCCTCTATGTCGCCAACGAGGGCAAGCTGATCTGTATCTGCGCCGAAGAGG
ACGCGGCCCGCCTGCTCGACATCATGCGCGCCCATCCCTTGGGCGCCGACGCGGCGCGCATCGGGGCGTGCCGGGAAGAT
TCCCACCACTTCGTCCAGATGAAGACCCGCATGGGCGGCAACCGGGTGGTGGACTGGCTGGCCGGCGAACAGTTGCCGAG
GATCTGCTGA

Upstream 100 bases:

>100_bases
TCCCGAACTGGGAACCATCTGCTGGCCCAACGGCGCCGACATTTGCCCCGACGTCCTTTATTCCCGCGTCACAGGCGCCC
CCCTTCCCGGAACACACTGA

Downstream 100 bases:

>100_bases
TGCACGTCTTGCTATTGGCCCATTCCTTCAACTCGCTGACCCAGCGCCTGTGGGTGGAACTGTCCGAGTTGGGCCACGAC
GTCACCTGCGAATTCGACGT

Product: hydrogenase expression/formation protein hypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTLPRRAHPRPLDIASGRVDLTHGGGGRAMAQLVEELFIAAFDNEALNQRNDQAAFDVAGGRMVMTTDGYVISPLFFPGG
DIGSLAVHGTVNDVAMAGARPISLSAGFIIEEGFPLSDLKRIVESMAKASKEAGVPVVTGDTKVVEKGKGDGIFITTAGI
GMVPPGVEISGDKARSGDAILVSGTMGDHGVAVMSQRESLGFETEILSDSAALHTMVAEMVAAVPGIRVLRDPTRGGLAA
TLNEIASQSGIGMLMEEALVPVKPQVLGACELLGLDPLYVANEGKLICICAEEDAARLLDIMRAHPLGADAARIGACRED
SHHFVQMKTRMGGNRVVDWLAGEQLPRIC

Sequences:

>Translated_349_residues
MTLPRRAHPRPLDIASGRVDLTHGGGGRAMAQLVEELFIAAFDNEALNQRNDQAAFDVAGGRMVMTTDGYVISPLFFPGG
DIGSLAVHGTVNDVAMAGARPISLSAGFIIEEGFPLSDLKRIVESMAKASKEAGVPVVTGDTKVVEKGKGDGIFITTAGI
GMVPPGVEISGDKARSGDAILVSGTMGDHGVAVMSQRESLGFETEILSDSAALHTMVAEMVAAVPGIRVLRDPTRGGLAA
TLNEIASQSGIGMLMEEALVPVKPQVLGACELLGLDPLYVANEGKLICICAEEDAARLLDIMRAHPLGADAARIGACRED
SHHFVQMKTRMGGNRVVDWLAGEQLPRIC
>Mature_348_residues
TLPRRAHPRPLDIASGRVDLTHGGGGRAMAQLVEELFIAAFDNEALNQRNDQAAFDVAGGRMVMTTDGYVISPLFFPGGD
IGSLAVHGTVNDVAMAGARPISLSAGFIIEEGFPLSDLKRIVESMAKASKEAGVPVVTGDTKVVEKGKGDGIFITTAGIG
MVPPGVEISGDKARSGDAILVSGTMGDHGVAVMSQRESLGFETEILSDSAALHTMVAEMVAAVPGIRVLRDPTRGGLAAT
LNEIASQSGIGMLMEEALVPVKPQVLGACELLGLDPLYVANEGKLICICAEEDAARLLDIMRAHPLGADAARIGACREDS
HHFVQMKTRMGGNRVVDWLAGEQLPRIC

Specific function: Unknown

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=337, Percent_Identity=47.1810089020772, Blast_Score=305, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 36561; Mature: 36429

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRRAHPRPLDIASGRVDLTHGGGGRAMAQLVEELFIAAFDNEALNQRNDQAAFDVAG
CCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEECC
GRMVMTTDGYVISPLFFPGGDIGSLAVHGTVNDVAMAGARPISLSAGFIIEEGFPLSDLK
CEEEEEECCEEEEEEECCCCCCCCEEEECCHHHHHHCCCCCEEEECCEEEECCCCHHHHH
RIVESMAKASKEAGVPVVTGDTKVVEKGKGDGIFITTAGIGMVPPGVEISGDKARSGDAI
HHHHHHHHHHHHCCCEEEECCHHEEECCCCCEEEEEECCCCCCCCCCEECCCCCCCCCEE
LVSGTMGDHGVAVMSQRESLGFETEILSDSAALHTMVAEMVAAVPGIRVLRDPTRGGLAA
EEECCCCCCCEEEEEHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
TLNEIASQSGIGMLMEEALVPVKPQVLGACELLGLDPLYVANEGKLICICAEEDAARLLD
HHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEECCCHHHHHHH
IMRAHPLGADAARIGACREDSHHFVQMKTRMGGNRVVDWLAGEQLPRIC
HHHCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCCEEHHHHCCCCCCCC
>Mature Secondary Structure 
TLPRRAHPRPLDIASGRVDLTHGGGGRAMAQLVEELFIAAFDNEALNQRNDQAAFDVAG
CCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEECC
GRMVMTTDGYVISPLFFPGGDIGSLAVHGTVNDVAMAGARPISLSAGFIIEEGFPLSDLK
CEEEEEECCEEEEEEECCCCCCCCEEEECCHHHHHHCCCCCEEEECCEEEECCCCHHHHH
RIVESMAKASKEAGVPVVTGDTKVVEKGKGDGIFITTAGIGMVPPGVEISGDKARSGDAI
HHHHHHHHHHHHCCCEEEECCHHEEECCCCCEEEEEECCCCCCCCCCEECCCCCCCCCEE
LVSGTMGDHGVAVMSQRESLGFETEILSDSAALHTMVAEMVAAVPGIRVLRDPTRGGLAA
EEECCCCCCCEEEEEHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
TLNEIASQSGIGMLMEEALVPVKPQVLGACELLGLDPLYVANEGKLICICAEEDAARLLD
HHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEECCCHHHHHHH
IMRAHPLGADAARIGACREDSHHFVQMKTRMGGNRVVDWLAGEQLPRIC
HHHCCCCCCCHHHHCCCCCCCCEEEEEEECCCCCCEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8352644; 12948488; 8021224 [H]