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The map label for this gene is nifE [H]

Identifier: 83310668

GI number: 83310668

Start: 1697214

End: 1698596

Strand: Reverse

Name: nifE [H]

Synonym: amb1569

Alternate gene names: 83310668

Gene position: 1698596-1697214 (Counterclockwise)

Preceding gene: 83310669

Following gene: 83310667

Centisome position: 34.2

GC content: 67.1

Gene sequence:

>1383_bases
ATGCTGAAGGCCAAGATCGCCGAACTGATGCACGAACCCGGATGCGCCACCAACCAGGCGAAATCCGAAGGTGAGCGCAA
GAAGGGCTGCGGCAAGTCGCTCACCCCCGGCGCCGCCGCCGGCGGCTGCGCCTTCGACGGTGCCAAGATCGCGCTCCAGC
CCATCACCGACGTGGTCCATCTCGTCCACGGCCCCATCGCCTGCGAGGGCAATTCCTGGGACGGCCGTAATTCCTGGAGC
AGCGGCTCGGACCTCTACCGCAAGGGCTTCACCACCGATCTGACCAACCTGGACATTATCGCCGGTGGCGAGAAGAAGCT
CTACAAGGCCATCAAGCAGGCCATCGCCCGCCACAACCCGCCCGCCGTCTTCGTCTACCAGACCTGTGTCGGCTCGCTGA
TCGGCGACGACGTGGACGCCGTGTGCCGCGCCGCCACCCAGCGCCTGGGCACCCCGGTGATCCCGATCAATTCGCCGGGC
TTCGTCGGCTCGAAGAACCTGGGCAACCGCTTAGGCGGCGAGGCGCTGTTCGAGCATGTGATCGGCACGGTGGAGCCCGA
CGATGCCGGCCCCTGCGACATCAATATCCTGGGCGAGTACAACGTGGCGGGCGAGCTGGACCAGTTCCGCCCCCTGCTGG
CCCGCCTGGGGATCCGCCTGCGCGCCTCCATCACCGGCGACGCCCGCTATCGCGAGGTGGCGGCCTGCCACACGGCGCGC
GCCAACATGGTGCTGTGCTCCCAGGCCCTGGTGACGCTCGCCCGCAAGATGAAGGAGAAGTGGGGCATCCCCTACTTCGA
AGGCTCGTTCTACGGCATCTCCGACACCTCGGACGCCCTGCGCAACATCGCCCGCATGCTGGTGGAGCGCGGCGCCGATC
CCACCCTGACCGACCGCACCGAGGCTCTGATCGCCGAGGAAGAGGCCAAGGCCTGGGCGCGGCTGGAGCCCTACAAGGCC
CGTCTGGCGGGCCGCAAGGTGCTGCTTTACACCGGCGGCGTCAAAAGCTGGTCGGTGATCCAGGCCCTGCAGGAAGTGGG
CATGGAAGTGGTGGGCTCCTCGGTCCGCAAGGCCACCGAGGCCGACAAGTCCAAGGCGCTGGAACGTCTGGGCGGCGACG
AGGACAAGCTGGCCGACGCCATCACGCCGCGCGACATGTACGCCATGTTCAAGGACGGCAAGGCCGACATGATGCTGTCG
GGCGGACGTTCGCAATTCGTGGCGCTGAAGGCCCGCACCCCCTGGATCGACATCAATCAGGAACGCCACCACGGCTATGC
CGCCTATGACGGCATGGTGGCCCTGGTGCGCGAGATCGATCTTTCCATCAACAGCCCGATCTGGGCGCAGGTCCGCGCCC
CCGCCCCCTGGGAGAATTCGTGA

Upstream 100 bases:

>100_bases
GGCCGGTTGCCGTTTGCGCACCCCCCAGCATTTCCGCCACTTTTACTAATGGCACGGCCCTTGCGAGTCAGGACCCGAAC
CGCTTTACGCGAGGTCCGCC

Downstream 100 bases:

>100_bases
TGGCCCTTATCGTCGACCACAACAAGGCGCTTTCCACCTCTCCGCTCAAGGTCAGCGCGCCGCTGGGCGCGGCGCTGGCC
TTCATGGGGATGAAGGGCTG

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: oxidized ferredoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLKAKIAELMHEPGCATNQAKSEGERKKGCGKSLTPGAAAGGCAFDGAKIALQPITDVVHLVHGPIACEGNSWDGRNSWS
SGSDLYRKGFTTDLTNLDIIAGGEKKLYKAIKQAIARHNPPAVFVYQTCVGSLIGDDVDAVCRAATQRLGTPVIPINSPG
FVGSKNLGNRLGGEALFEHVIGTVEPDDAGPCDINILGEYNVAGELDQFRPLLARLGIRLRASITGDARYREVAACHTAR
ANMVLCSQALVTLARKMKEKWGIPYFEGSFYGISDTSDALRNIARMLVERGADPTLTDRTEALIAEEEAKAWARLEPYKA
RLAGRKVLLYTGGVKSWSVIQALQEVGMEVVGSSVRKATEADKSKALERLGGDEDKLADAITPRDMYAMFKDGKADMMLS
GGRSQFVALKARTPWIDINQERHHGYAAYDGMVALVREIDLSINSPIWAQVRAPAPWENS

Sequences:

>Translated_460_residues
MLKAKIAELMHEPGCATNQAKSEGERKKGCGKSLTPGAAAGGCAFDGAKIALQPITDVVHLVHGPIACEGNSWDGRNSWS
SGSDLYRKGFTTDLTNLDIIAGGEKKLYKAIKQAIARHNPPAVFVYQTCVGSLIGDDVDAVCRAATQRLGTPVIPINSPG
FVGSKNLGNRLGGEALFEHVIGTVEPDDAGPCDINILGEYNVAGELDQFRPLLARLGIRLRASITGDARYREVAACHTAR
ANMVLCSQALVTLARKMKEKWGIPYFEGSFYGISDTSDALRNIARMLVERGADPTLTDRTEALIAEEEAKAWARLEPYKA
RLAGRKVLLYTGGVKSWSVIQALQEVGMEVVGSSVRKATEADKSKALERLGGDEDKLADAITPRDMYAMFKDGKADMMLS
GGRSQFVALKARTPWIDINQERHHGYAAYDGMVALVREIDLSINSPIWAQVRAPAPWENS
>Mature_460_residues
MLKAKIAELMHEPGCATNQAKSEGERKKGCGKSLTPGAAAGGCAFDGAKIALQPITDVVHLVHGPIACEGNSWDGRNSWS
SGSDLYRKGFTTDLTNLDIIAGGEKKLYKAIKQAIARHNPPAVFVYQTCVGSLIGDDVDAVCRAATQRLGTPVIPINSPG
FVGSKNLGNRLGGEALFEHVIGTVEPDDAGPCDINILGEYNVAGELDQFRPLLARLGIRLRASITGDARYREVAACHTAR
ANMVLCSQALVTLARKMKEKWGIPYFEGSFYGISDTSDALRNIARMLVERGADPTLTDRTEALIAEEEAKAWARLEPYKA
RLAGRKVLLYTGGVKSWSVIQALQEVGMEVVGSSVRKATEADKSKALERLGGDEDKLADAITPRDMYAMFKDGKADMMLS
GGRSQFVALKARTPWIDINQERHHGYAAYDGMVALVREIDLSINSPIWAQVRAPAPWENS

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: 1.18.6.1

Molecular weight: Translated: 49745; Mature: 49745

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKAKIAELMHEPGCATNQAKSEGERKKGCGKSLTPGAAAGGCAFDGAKIALQPITDVVH
CCHHHHHHHHHCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHH
LVHGPIACEGNSWDGRNSWSSGSDLYRKGFTTDLTNLDIIAGGEKKLYKAIKQAIARHNP
HHCCCEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCC
PAVFVYQTCVGSLIGDDVDAVCRAATQRLGTPVIPINSPGFVGSKNLGNRLGGEALFEHV
CEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHCCHHHHHHHH
IGTVEPDDAGPCDINILGEYNVAGELDQFRPLLARLGIRLRASITGDARYREVAACHTAR
HCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHH
ANMVLCSQALVTLARKMKEKWGIPYFEGSFYGISDTSDALRNIARMLVERGADPTLTDRT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH
EALIAEEEAKAWARLEPYKARLAGRKVLLYTGGVKSWSVIQALQEVGMEVVGSSVRKATE
HHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ADKSKALERLGGDEDKLADAITPRDMYAMFKDGKADMMLSGGRSQFVALKARTPWIDINQ
HHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCEEEEEEECCCEEECCC
ERHHGYAAYDGMVALVREIDLSINSPIWAQVRAPAPWENS
CCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MLKAKIAELMHEPGCATNQAKSEGERKKGCGKSLTPGAAAGGCAFDGAKIALQPITDVVH
CCHHHHHHHHHCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHH
LVHGPIACEGNSWDGRNSWSSGSDLYRKGFTTDLTNLDIIAGGEKKLYKAIKQAIARHNP
HHCCCEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCC
PAVFVYQTCVGSLIGDDVDAVCRAATQRLGTPVIPINSPGFVGSKNLGNRLGGEALFEHV
CEEEEHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHCCHHHHHHHH
IGTVEPDDAGPCDINILGEYNVAGELDQFRPLLARLGIRLRASITGDARYREVAACHTAR
HCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHH
ANMVLCSQALVTLARKMKEKWGIPYFEGSFYGISDTSDALRNIARMLVERGADPTLTDRT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH
EALIAEEEAKAWARLEPYKARLAGRKVLLYTGGVKSWSVIQALQEVGMEVVGSSVRKATE
HHHHHHHHHHHHHHCCHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ADKSKALERLGGDEDKLADAITPRDMYAMFKDGKADMMLSGGRSQFVALKARTPWIDINQ
HHHHHHHHHHCCCHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCEEEEEEECCCEEECCC
ERHHGYAAYDGMVALVREIDLSINSPIWAQVRAPAPWENS
CCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; H+; N2; ATP

Specific reaction: 3 reduced ferredoxin + 6 H+ + N2 + n ATP = 3 oxidized ferredoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]